Measurement of tissue oxygen with a fluorescent probe

1976 ◽  
Vol 41 (4) ◽  
pp. 598-602 ◽  
Author(s):  
I. S. Longmuir ◽  
J. A. Knopp
Keyword(s):  
Oxygen Consumption ◽  
Fluorescence Quenching ◽  
Fluorescent Probe ◽  
Spatial Resolution ◽  
Tissue Oxygen ◽  
Quenching Of Fluorescence ◽  
Oxygen Gradients ◽  
Geometrical Factors

Evidence is presented that pyrenebutyric acid is nontoxic at the concentrations required for the measurement of fluorescence quenching by physiological concentrations of oxygen. It appears that this molecule can be used tomeasure tissue oxygen since it does not interfere with oxygen consumption. Equally, tissue components do not interfere with the quenching of fluorescence to such an extent as to invalidate the method. The spatial resolution of the technique is limited by some tissue geometrical factors which do not,however, appear to be great enough to prevent its use to measure intercapillary oxygen gradients.

An acidity scale, [H+]hv, for proton quenching of excited states

1989 ◽  
Vol 67 (2) ◽  
pp. 227-238 ◽  
Author(s):  
J. A. Pincock ◽  
P. R. Redden
Keyword(s):  
Excited State ◽  
Fluorescence Quenching ◽  
Excited States ◽  
Benzyl Alcohols ◽  
Fluorescent Indicator ◽  
Quenching Of Fluorescence ◽  
Acidity Scale

An acidity scale for excited state protonation kinetics in 20% ethanol:80% aqueous sulfuric has been developed using 1-cyano-naphthalene as a fluorescent indicator. The utility of this new scale is demonstrated using the proton quenching of fluorescence of a variety of chromophores. These include 1- and 2-cyanonaphthalenes, 1- and 2-methoxynaphthalenes, benzyl alcohols, toluenes, and 2-vinylnaphthalene. Keywords: acidity scale, fluorescence quenching, excited state basicity.

Measurement of tissue oxygen consumption in patients with mitochondrial myopathy by noninvasive tissue oximetry

Neurology ◽  
1997 ◽  
Vol 49 (3) ◽  
pp. 837-841 ◽  
Author(s):  
K. Abe ◽  
Y. Matsuo ◽  
J. Kadekawa ◽  
S. Inoue ◽  
T. Yanagihara
Keyword(s):  
Oxygen Consumption ◽  
Mitochondrial Myopathy ◽  
Tissue Oxygen ◽  
Tissue Oximetry ◽  
Tissue Oxygen Consumption

Local blood flow, oxygen tension, and oxygen consumption in the rat spinal cord

1983 ◽  
Vol 58 (4) ◽  
pp. 526-530 ◽  
Author(s):  
Nariyuki Hayashi ◽  
Barth A. Green ◽  
Mayra Gonzalez-Carvajal ◽  
Joseph Mora ◽  
Richard P. Veraa
Keyword(s):  
Spinal Cord ◽  
Blood Flow ◽  
Oxygen Consumption ◽  
Oxygen Tension ◽  
Tissue Oxygen ◽  
Local Blood Flow ◽  
Rat Spinal Cord ◽  
Spinal Cord Blood Flow ◽  
Content Type ◽  
Tissue Oxygen Consumption

✓ Using a reliable and reproducible microelectrode technique, consistent simultaneous measurements of local spinal cord blood flow (SCBF), tissue oxygen tension, and tissue oxygen consumption were made at cervical, thoracic, and lumbar levels in the rat spinal cord. These observations showed that the metabolic state is maintained constant along the cord, despite significant variations in vasculature. The physiological and anatomical aspects of these findings are discussed.

An “on–off” fluorescent probe based on cucurbit[7]uril for highly sensitive determination of ammonia nitrogen in aquaculture water

2021 ◽  
Author(s):  
Zhen Li ◽  
Tan Wang ◽  
Xianbao Xu ◽  
Cong Wang ◽  
Daoliang Li
Keyword(s):  
Fluorescence Quenching ◽  
Inclusion Complex ◽  
Fluorescent Probe ◽  
Ammonia Nitrogen ◽  
Highly Sensitive ◽  
Sensitive Determination ◽  
Aquaculture Water

A novel “on–off” fluorescent probe for the determination of ammonia nitrogen has been synthesized. URO can replace PAL into the cavity of CB[7] to form a stable inclusion complex, eventually forming the fluorescence quenching system of URO@CB[7].

Mitochondrial respiratory control can compensate for intracellular O2 gradients in cardiomyocytes at low Po 2

2002 ◽  
Vol 283 (3) ◽  
pp. H871-H878 ◽  
Author(s):  
Eiji Takahashi ◽  
Koji Asano
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Diffusion ◽  
Respiratory Control ◽  
Oxygen Gradients ◽  
Nadh Fluorescence ◽  
Carbonyl Cyanide ◽  
Intrinsic Regulation ◽  
Electrical Pacing ◽  
Mitochondrial Oxidative Metabolism ◽  
Mitochondrial Respiratory

In isolated single cardiomyocytes with moderately elevated mitochondrial respiration, direct evidence for intracellular radial gradients of oxygen concentration was obtained by subcellular spectrophotometry of myoglobin (Mb). When oxygen consumption was increased by carbonyl cyanide m-chlorophenylhydrazone (CCCP) during superfusion of cells with 4% oxygen, Po 2 at the cell core dropped to 2.3 mmHg, whereas Mb near the plasma membrane was almost fully saturated with oxygen. Subcellular NADH fluorometry demonstrated corresponding intracellular heterogeneities of NADH, indicating suppression of mitochondrial oxidative metabolism due to relatively slow intracellular oxygen diffusion. When oxygen consumption was increased by electrical pacing in 2% oxygen, radial oxygen gradients of similar magnitude were demonstrated (cell core Po 2 = 2.6 mmHg). However, an increase in NADH fluorescence at the cell core was not detected. Because CCCP abolished mitochondrial respiratory control while it was intact in electrically paced cardiomyocytes, we conclude that mitochondria with intact respiratory control can sustain electron transfer with reduced oxygen supply. Thus mitochondrial intrinsic regulation can compensate for relatively slow oxygen diffusion within cardiomyocytes.

Sensing of tryptophan by a non-toxic cobalt(ii) complex

RSC Advances ◽  
2016 ◽  
Vol 6 (98) ◽  
pp. 95888-95896 ◽  
Author(s):  
Swapan Kumar Jana ◽  
Amit Kumar Mandal ◽  
Anoop Kumar ◽  
Horst Puschmann ◽  
Maidul Hossain ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Fluorescence Quenching ◽  
Fluorescent Probe ◽  
First Report

The first report of a cobalt(ii) based non-toxic, hemocompatible, fluorescent probe that sense Trp and BSA by reducing internal fluorescence quenching of Trp in aqueous solution.

Effect of erythrocyte storage and oxyhemoglobin affinity changes on cardiac function

1985 ◽  
Vol 248 (4) ◽  
pp. H508-H515 ◽  
Author(s):  
C. S. Apstein ◽  
R. C. Dennis ◽  
L. Briggs ◽  
W. M. Vogel ◽  
J. Frazer ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Coronary Flow ◽  
Myocardial Oxygen Consumption ◽  
Myocardial Function ◽  
Contractile Function ◽  
Storage Conditions ◽  
Tissue Oxygen ◽  
Biochemical Treatment ◽  
Stress States ◽  
Stored Blood

Storage of blood can depress erythrocyte 2,3-diphosphoglycerate (DPG) levels and thereby increase oxyhemoglobin affinity and potentially decrease capillary-to-tissue oxygen transport. We measured myocardial function and metabolism in isolated rabbit hearts with fixed coronary flow under basal conditions and during isoproterenol stress at 37 and 30 degrees C, comparing high and low oxyhemoglobin affinity (OHA) erythrocytes. The high OHA state resulted from standard storage conditions, which caused depressed values of DPG and P50 (the oxygen tension at which hemoglobin is 50% saturated). The low OHA erythrocytes were initially stored and then underwent biochemical treatment to restore the DPG and P50 values to normal. The low OHA cells released more oxygen, and myocardial oxygen consumption and contractile function were increased relative to the high OHA cells during both the basal and stress states at both 37 and 30 degrees C. These observations may be relevant for patients with limited coronary flow when such patients receive large transfusions of stored blood.

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