Chylomicron triglyceride metabolism in resting and exercising fed dogs

1982 ◽  
Vol 52 (4) ◽  
pp. 815-820 ◽  
Author(s):  
R. L. Terjung ◽  
L. Budohoski ◽  
K. Nazar ◽  
A. Kobryn ◽  
H. Kaciuba-Uscilko
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Corn Oil ◽  
Removal Rate ◽  
Moderate Intensity ◽  
Beta Oxidation ◽  
Fed State ◽  
Acid Pool ◽  
Fatty Acid Pool

The turnover of circulating triglycerides (TG) was determined in dogs during rest, following ingestion of food that included corn oil, and in the final period of a 1-h treadmill exercise of moderate intensity (72–84 m/min). In all cases the loss of [14C]TG from the plasma followed a first-order process. The fractional removal rate constant at rest was 26.5 +/- 1.9% (SE) n = 10) of the circulating pool size per minute, and it was increased slightly to 33.8 +/- 3.6% (n = 7) per minute during exercise. The uptake of plasma TG-derived fatty acids (5 min postinjection) was increased (P less than 0.05) in working muscle, whereas the TG uptake in fat tended to decrease. Further, the percent of TG-derived fatty acids found in the muscle's acylglyceride pool was less (90.0 +/- 3.6 vs. 53.5 +/- 1.8%), while that in the muscle's free fatty acid pool was greater (12.3 +/- 36.1 +/- 4.7%) in working compared with resting muscle. Thus the fourfold greater quantity of plasma TG-derived fatty acids found in the working muscle's free fatty acid pool could account for the entire increased TG uptake caused by exercise. This suggests that, in the fed state, circulating TG could represent a potential source of fatty acids for beta-oxidation in working muscle. However, the importance of plasma TG-derived fatty acids as an energy substrate during muscle use in a postprandial state has yet to be determined quantitatively.

The subcellular distribution of free fatty acids released during post-decapitative ischemia in rat cerebral cortex

1985 ◽  
Vol 63 (11) ◽  
pp. 1183-1188 ◽  
Author(s):  
R. Roy Baker ◽  
Zou Dao Loh
Keyword(s):  
Fatty Acids ◽  
Cerebral Cortex ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Subcellular Distribution ◽  
Phospholipase A ◽  
C Cells ◽  
Acid Pool ◽  
Fatty Acid Pool

After periods of 5 and 30 min following decapitation, rat cerebral cortices were removed and subcellular fractions were prepared. Fractions P1A (large myelin), P1B (nuclei), P1C (cells and debris), P2A (small myelin), P2B (synaptosomes), P2C (mitochondria), and P3 (microsomes) were isolated. Free fatty acid levels of 1.0 and 1.4 μmol/g tissue were found in the homogenates at the early and late times of ischemia. In the 30-min samples, P1A, P1C, and P2A had relatively high specific contents of total free fatty acids in comparison with other subfractions. At this time P2C was relatively enriched in arachidonate, P1A and P2A were enriched in palmitate, and P2B and P3 were enriched in stearate in comparison with the homogenate. P2C had the highest ratio of polyunsaturates/saturates in its free fatty acid pool. Comparing the 5- and 30-min samples, a large increase in the quantity of free fatty acids was found in fractions P1A and P2A, so that at the later time P1A + P2A contained 60 mol% of the free fatty acid in the total subfractions derived from cerebral cortex. In comparison with the homogenate, the lack of accumulation of free fatty acids in certain membranes known to possess phospholipase activities (e.g., phospholipase A2 in P2C) and the buildup of free fatty acids in P1A and P2A led to the hypothesis that free fatty acids may be migrating outwards from intracellular sites of production and accumulating in the multilamellar structure of myelin.

scholarly journals Lipolysis and the Free Fatty Acid Pool in Seedlings

1964 ◽  
Vol 39 (6) ◽  
pp. 880-883 ◽  
Author(s):  
Allen J. St. Angelo ◽  
Aaron M. Altschul
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Acid Pool ◽  
Fatty Acid Pool

scholarly journals Jurnal_TEDC Vol. 11 No. 1, Januari 2017

2019 ◽  
Author(s):  
Lusi Marlina
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Olive Oil ◽  
Corn Oil ◽  
Saturated Fatty Acids ◽  
Palm Fruit ◽  
Cooking Oil ◽  
Omega 6 ◽  
Fruit Oil

IDENTIFIKASI KADAR ASAM LEMAK BEBAS PADA BERBAGAI JENISMINYAK GORENG NABATILusi Marlina1, Imam Ramdan21,2 Teknik Kimia – Politeknik TEDC BandungEmail : [email protected] minyak goreng untuk mengolah makanan sangat banyak dipergunakan oleh masyarakat.Minyak goreng berasal dari bahan baku seperti: kelapa, kelapa sawit, jagung, kedelai, buah zaitun, dan lainlain. Kandungan utama dari minyak goreng secara umum adalah asam lemak yang terdiri dari asam lemakjenuh (saturated fatty acids) misalnya: asam plamitat, asam stearat, dan asam lemak tak jenuh (unsaturatedfatty acids) misalnya: asam oleat (Omega 9) dan asam linoleat (Omega 6). Asam lemak bebas merupakanasam lemak yang tidak terikat sebagai trigliserida yang dapat terbentuk karena adanya reaksi hidrolisis didalam minyak. Asam lemak yang berlebihan di dalam tubuh dapat memicu terjadinya kanker karena bersifatkarsinogen. Penelitian ini bertujuan untuk mengidentifikasi kadar asam lemak bebas pada minyak gorengnabati, dengan menggunakan metode titrasi asam basa. Titrasi asam basa yaitu suatu analisis kuantitatifuntuk menetapkan kadar senyawa-senyawa yang bersifat asam, dengan menggunakan larutan basa sebagaistandar. Analisis fisika meliputi warna, aroma dan massa jenis, sedangkan analisa kimia berdasarkan kadarasam lemak bebas. Hasil dari penelitian diperoleh : kadar asam lemak bebas dari berbagai sampel minyaknabati yang terdiri dari: minyak jagung sebesar 0,22%, minyak sawit sebesar 0,16%, VCO sebesar 0,25%dan minyak zaitun sebesar 0,21%, sedangkan untuk massa jenis minyak yaitu: minyak jagung sebesar1,01gr/ml, minyak sawit sebesar 1,04gr/ml, VCO sebesar 0,97gr/ml, dan minyak zaitun sebesar 0,90gr/ml.Secara umum dapat disimpulkan bahwa minyak goreng nabati yang diteliti tidak melebihi standar SNI 3741-1995 yang ditetapkan sebesar 0,30%.Kata kunci: minyak goreng nabati, asam lemak bebas, hidrolisis, titrasi asam basa.AbstractThe use of cooking oil to proceed food is very common in daily cooking. Vegetable Cooking oil is made ofsubstance like: coconut, pal oil, corn, soybean, sunflower seeds, and others. The prominent content ofcooking oil commonly is fatty acid which consists of saturated fatty acids, as: plamitat acid, stearat acid; andunsaturated fatty acids as: oleat acid (Omega 9) and linoleat acid (Omega 6). Free fatty acid is untied fattyacid as triglyceride that can be formed as a result of hydrolysis reaction in cooking oil. The abundant fattyacid in body can cause cancer because it is carcinogenic. The purpose of the research is to identify free fattyacid levels in vegetable cooking oil, by using alkalimeter method. Alkalimeter is a quantitative analysis todetermine level of acid compounds, using standard alkali solution. Physical analysis includes, smell andweight, meanwhile chemical analysis based on free fatty acid levels. The result of the research is: free fattyacid levels of various vegetable cooking oil samples those are: corn oil about 0,22%, palm fruit oil about0,16%, VCO about 0,25%, and olive oil about 00,21%, whereas for the weights, those are: corn oil about0,01gr/ml, palm fruit oil about 0,04gr/ml, VCO about 0,97gr/ml, and olive oil about 0,90gr/ml. It can generallybe concluded that examined vegetable cooking oil still meet the standard of SNI 3741-1995 that is 0,30%.Keyword: vegetable cooking oil, saturated fatty acid, hydrolysis, alkalimeter.

scholarly journals Flavour development via lipolysis of milkfats: changes in free fatty acid pool

2007 ◽  
Vol 42 (8) ◽  
pp. 961-968 ◽  
Author(s):  
Mafalda A. Regado ◽  
Betina M. Cristóvão ◽  
Carla G. Moutinho ◽  
Victor M. Balcão ◽  
Raquel Aires-Barros ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Acid Pool ◽  
Fatty Acid Pool

Mode of action of choline. II. The fate of intravenously administered labelled fatty acids in choline-deficient rats

1969 ◽  
Vol 47 (2) ◽  
pp. 207-218 ◽  
Author(s):  
A. Chalvardjian
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Total Phospholipid ◽  
Hepatic Triglyceride ◽  
Rat Serum ◽  
Short Period ◽  
Time Periods ◽  
Phospholipid Pool ◽  
Fatty Acid Pool

Two groups of rats (200 g, male) were fed, respectively, a choline-deficient and a choline-supplemented diet for 3 days. On the 3rd day each rat was injected intravenously with rat serum containing 9,10-3H-palmitate and 18-14C-stearate. The fate of the labelled fatty acids was followed in the triglycerides and phospholipids of liver and serum for time periods ranging from 1 min to 6 h. When compared to the choline-supplemented controls, the livers in the choline-deficient rats were fatty and were characterized by: (1) an increased hepatic triglyceride pool, (2) a decrease in turnover of hepatic triglycerides with sequestration of fatty acids and decrease in their recycling, (3) an increase in the hepatic free fatty acid pool and total phospholipid pool, the latter reflecting the net result of synthesis and transport, (4) a decrease in the early and rapid turnover of the phosphatidylcholine pool, (5) a decrease in turnover of the phosphatidylethanolamine pool, (6) a decrease in transport of triglycerides and phospholipids from liver to serum, and consequently, (7) a decrease in triglyceride and phospholipid pools in serum. These results confirm previous observations that, although an impairment of transport of both triglycerides and phospholipids from liver to serum exists in choline-deficient rats, the hepatic accumulation of triglycerides is far greater than that of phospholipids. This effect is due to the presence of a potential triglyceride pool in liver that can accommodate large amounts of triglyceride within a short period of time.

Effect of increased plasma free fatty acid concentrations on muscle metabolism in exercising men

1991 ◽  
Vol 70 (1) ◽  
pp. 194-201 ◽  
Author(s):  
M. Hargreaves ◽  
B. Kiens ◽  
E. A. Richter
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Carbohydrate Metabolism ◽  
Ketone Bodies ◽  
Plasma Concentrations ◽  
Plasma Free Fatty Acid ◽  
Substrate Utilization ◽  
Moderate Intensity

The effect of increasing plasma concentrations of free fatty acids on substrate utilization in muscle during exercise was investigated in 11 healthy young males. One hour of dynamic knee extension at 80% of knee-extensor maximal work capacity was performed first with one leg and then with the other leg during infusion of Intralipid and heparin. Substrate utilization was assessed from arterial and femoral venous blood sampling as well as from muscle biopsies. Intralipid infusion increased mean plasma free fatty acid concentrations from 0.54 +/- 0.08 to 1.12 +/- 0.09 (SE) mM. Thigh glucose uptake during rest, exercise, and recovery was decreased by 64, 33, and 42%, respectively, by Intralipid, whereas muscle glycogen breakdown and release of lactate, pyruvate, and citrate were unaffected. Concentrations of glucose, glucose 6-phosphate, and lactate in muscle before and at termination of exercise were unaffected by Intralipid. During exercise, net leg uptake of plasma free fatty acids was not measurably increased by Intralipid, whereas uptake of ketone bodies was. Local respiratory quotient across the leg was not changed by Intralipid (control 0.87 +/- 0.02, Intralipid 0.86 +/- 0.02). Arterial concentrations of insulin, norepinephrine, and epinephrine were similar in the two trials. It is concluded that at rest and during exercise at a moderate intensity (requiring approximately equal contributions from fat and carbohydrate metabolism), muscle carbohydrate metabolism is affected only with regard to uptake of glucose when plasma concentrations of lipid and lipid metabolites are increased. This effect may be by direct inhibition of glucose transport rather than by the classic glucose-fatty acid cycle.

Sign in / Sign up

Export Citation Format

Share Document