Changes in tracheal cross-sectional area during Mueller and Valsalva maneuvers in humans

1986 ◽  
Vol 60 (6) ◽  
pp. 1865-1870 ◽  
Author(s):  
I. G. Brown ◽  
P. M. Webster ◽  
N. Zamel ◽  
V. Hoffstein
Keyword(s):  
Transmural Pressure ◽  
Positive Pressure ◽  
Pressure Gradients ◽  
Cross Sectional ◽  
Thoracic Inlet ◽  
Acoustic Reflection ◽  
Pressure Area ◽  
The Difference ◽  
Cervical Trachea

Pressure-area behavior of the excised trachea is well documented, but little is known of tracheal compliance in vivo. Extratracheal tissue pressures are not directly measurable, but transmural pressure for the intrathoracic trachea is inferred from intra-airway and pleural pressure differences. Extramural pressure of the cervical trachea is assumed to be atmospheric. The difference in transmural pressure between the intra- and extrathoracic tracheal segments should be exaggerated during Mueller and Valsalva maneuvers. We used the acoustic reflection technique to measure tracheal areas above and below the thoracic inlet during these isovolume-pressure maneuvers. We found that 10 cmH2O positive pressure increased tracheal area in the extrathoracic segment by 34 +/- 16% (mean +/- SD) and in the intrathoracic segment by 35 +/- 15%. There was a reduction in area of 27 +/- 16 and 24 +/- 14%, respectively, for the extra- and intrathoracic segments with 10 cmH2O negative pressure. We conclude that the effective transmural pressure gradients do not vary significantly between intra- and extrathoracic tracheal segments.

In vivo estimation of tracheal distensibility and hysteresis in normal adults

1987 ◽  
Vol 63 (6) ◽  
pp. 2482-2489 ◽  
Author(s):  
V. Hoffstein ◽  
R. G. Castile ◽  
C. R. O'Donnell ◽  
G. M. Glass ◽  
D. J. Strieder ◽  
...  
Keyword(s):  
Lung Volume ◽  
Transpulmonary Pressure ◽  
Lung Parenchyma ◽  
Esophageal Pressure ◽  
Transmural Pressure ◽  
Variable Degree ◽  
Cross Sectional ◽  
Acoustic Reflection ◽  
Direct Measurements

We used the acoustic reflection technique to measure the cross-sectional area of tracheal and bronchial airway segments of eight healthy adults. We measured airway area during a slow continuous expiration from total lung capacity (TLC) to residual volume (RV) and during inspiration back to TLC. Lung volume and esophageal pressure were monitored continuously during this quasi-static, double vital capacity maneuver. We found that 1) the area of tracheal and bronchial segments increases with increasing lung volume and transpulmonary pressure, 2) the trachea and bronchi exhibit a variable degree of hysteresis, which may be greater or less than that of the lung parenchyma, 3) extrathoracic and intrathoracic tracheal segments behaved as if they were subjected to similar transmural pressure and had similar elastic properties, and 4) specific compliance (means +/- SE) for the intrathoracic and bronchial segments, calculated with the assumption that transmural pressure is equal to the transpulmonary pressure, was significantly (P less than 0.05) smaller for the intrathoracic segment than for the bronchial segment: (2.1 +/- 2.0) X 10(-3) cmH2O-–1 vs. (9.1 +/- 2.1) X 10(-3) cmH2O–1. Direct measurements of airway area using acoustic reflections are in good agreement with previous estimates of airway distensibility in vivo, obtained by radiography or endoscopy.

Noninvasive Estimate of Work of Breathing Due to the Endotracheal Tube

1996 ◽  
Vol 85 (6) ◽  
pp. 1324-1333. ◽  
Author(s):  
Laurent Heyer ◽  
Bruno Louis ◽  
Daniel Isabey ◽  
Frederic Lofaso ◽  
Laurent Brochard ◽  
...  
Keyword(s):  
Pressure Drop ◽  
Endotracheal Tube ◽  
Pressure Support ◽  
Work Of Breathing ◽  
Reference Value ◽  
Cross Sectional ◽  
Acoustic Reflection ◽  
The Difference ◽  
Drop Flow

Background Although evidence suggests that secretions lining the inner wall of the endotracheal tube (ETT) often reduce its cross-sectional area, no data are available on the work of breathing as affected by the ETT. A noninvasive method is proposed for estimating the additional work of breathing necessitated by the ETT in patients whose lungs are mechanically ventilated. This method (the acoustic-Blasius method) involves (1) determining the inner geometry of the ETT using the acoustic reflection method and (2) using these geometric data to solve the Blasius equation that characterizes the ETT pressure drop-flow relation. Methods To evaluate the acoustic-Blasius method in vivo, the authors computed the work of breathing due to the ETT in four healthy persons breathing through an ETT connected to a pressure-support device and in five tracheally intubated patients receiving mechanical assistance in the pressure-support mode. For the tracheally intubated patients, the reference value was the work calculated from the ETT pressure drop measured between the two ends of the ETT using a pressure catheter. Results In the healthy participants and the tracheally intubated patients, there was close agreement between inspiratory work per cycle values estimated by directly measuring the ETT pressure drop and calculated using the acoustic-Blasius method: The difference was consistently less than 0.08 joules (< 10% of the reference value). Conclusions The data show that the acoustic-Blasius method allows noninvasive quantification of the ETT-related work of breathing in situ.

scholarly journals The structure and dynamics of patch-clamped membranes: a study using differential interference contrast light microscopy.

1990 ◽  
Vol 111 (2) ◽  
pp. 599-606 ◽  
Author(s):  
M Sokabe ◽  
F Sachs
Keyword(s):  
Cell Surface ◽  
Transmural Pressure ◽  
Positive Pressure ◽  
Radius Of Curvature ◽  
Pressure Gradients ◽  
Structure And Motion ◽  
Excised Patches ◽  
Glass Interface ◽  
Pipette Tip ◽  
Flow Through

We have developed techniques for micromanipulation under high power video microscopy. We have used these to study the structure and motion of patch-clamped membranes when driven by pressure steps. Patch-clamped membranes do not consist of just a membrane, but rather a plug of membrane-covered cytoplasm. There are organelles and vesicles within the cytoplasm in the pipette tip of both cell-attached and excised patches. The cytoplasm is capable of active contraction normal to the plane of the membrane. With suction applied before seal formation, vesicles may be swept from the cell surface by shear stress generated from the flow of saline over the cell surface. In this case, patch recordings are made from membrane that was not originally present under the tip. The vesicles may break, or fuse and break, to form the gigasealed patch. Patch membranes adhere strongly to the wall of the pipette so that at zero transmural pressure the membranes tend to be normal to the wall. With transmural pressure gradients, the membranes generally become spherical; the radius of curvature decreasing with increasing pressure. Some patches have nonuniform curvature demonstrating that forces normal to the membrane may be significant. Membranes often do not respond quickly to changes in pipette pressure, probably because viscoelastic cytoplasm reduces the rate of flow through the tip of the pipette. Inside-out patches may be peeled from the walls of the pipette, and even everted (with positive pressure), without losing the seal. This suggests that the gigaseal is a distributed property of the membrane-glass interface.

Oil Flow in a Full Journal Bearing

1961 ◽  
Vol 83 (2) ◽  
pp. 312-314
Author(s):  
Donald F. Hays
Keyword(s):  
Flow Rate ◽  
Journal Bearing ◽  
Hydrodynamic Pressure ◽  
Leakage Rate ◽  
Positive Pressure ◽  
Pressure Gradients ◽  
Oil Film ◽  
Oil Flow ◽  
Pressure Area ◽  
Side Flow

An analysis was made of the oil flows occurring in a full journal bearing with a continuous oil film. The flow rate into the bearing was determined at the section of greatest clearance and the rate of outflow was determined at the section of least clearance. The rate of side flow or leakage rate was determined by considering the flow across the boundary of the positive pressure area only and is the flow resulting from the hydrodynamic pressure gradients. It does not include the effects of any specific oil feed mechanism.

Modulation of bat wing venule contraction by transmural pressure changes

1992 ◽  
Vol 262 (3) ◽  
pp. H625-H634 ◽  
Author(s):  
M. J. Davis ◽  
X. Shi ◽  
P. J. Sikes
Keyword(s):  
Venous Pressure ◽  
Transmural Pressure ◽  
The Body ◽  
Intraluminal Pressure ◽  
Positive Pressure ◽  
Cross Sectional ◽  
Series Of Experiments ◽  
Pressure Changes ◽  
Bat Wing

We tested the hypothesis that the frequency and amplitude of spontaneous venular contractions in the bat wing could be modulated by changes in transmural pressure. In one series of experiments, venous pressure in the wing was elevated by pressurizing a box containing the body of the animal while the wing was exposed to atmospheric pressure. During this time, venular diameters were continuously recorded using intravital microscopic techniques while venular pressures were measured through servo-null micropipettes. In another series of experiments, single venular segments were dissected from the wing, cannulated, and pressurized in vitro. The results from both experimental protocols were qualitatively similar; alterations in venous pressure over a narrow range (+/- 5 cmH2O from control) produced substantial changes in contraction frequency and amplitude. The product of frequency and cross-sectional area was maximal over the venous pressure range between 10 and 15 cmH2O. Venules demonstrated a rate-sensitive component in their reaction to rapid pressure changes, because contraction bursts occurred immediately after positive pressure steps and quiescent periods often occurred after negative pressure steps. We conclude that venular vasomotion in the bat wing is modulated by intraluminal pressure and involves a bidirectional, rate-sensitive mechanism. In addition, comparisons with arteriolar vasomotion studies suggest that venules are more sensitive to luminal pressure changes than arterioles.

Novel method for measurement of medium size arterial lumen area with an impedance catheter: in vivo validation

2005 ◽  
Vol 288 (4) ◽  
pp. H2014-H2020 ◽  
Author(s):  
Ghassan S. Kassab ◽  
Eugen R. Lontis ◽  
Arne Hørlyck ◽  
Hans Gregersen
Keyword(s):  
Surrounding Tissue ◽  
Medium Size ◽  
Conductance Catheter ◽  
Algebraic Equations ◽  
Cross Sectional ◽  
Arterial Lumen ◽  
The Mean ◽  
The Difference ◽  
Parallel Conductance

There is no doubt that the transformation of a cardiac catheter into a conductance catheter that allows reliable and accurate assessment of lumen cross-sectional area (CSA) will provide a powerful diagnostic and treatment tool for the invasive cardiologist. The objective of this study was to develop a method based on the impedance catheter that allows accurate and reproducible measurements of CSA for medium size vessels (e.g., coronary, femoral, and carotid arteries). Two solutions of NaCl (0.5% and 1.5%) with known conductivities were injected directly into the lumen of the artery in eight swine. We showed that the CSA can be determined analytically from two Ohm's law-type algebraic equations that account for the parallel conductance of the current into the surrounding tissue. Excellent agreement was found between the conductance catheter with the proposed two-injection method and B-mode ultrasound (US). The root mean square error for the impedance measurements was 4.8% of the mean US diameter. The repeatability of the technique was assessed with duplicate measurements. The mean of the difference between the two measurements was nearly zero, and the repeatability coefficient was within 2.4% of the mean of the two measurements. The validated method was used to assess the degree of acute vasodilatation of the vessel in response to flow overload.

Asymmetric reflex responses of the nasal and tracheal vasculatures of the dog

1993 ◽  
Vol 75 (5) ◽  
pp. 2157-2161 ◽  
Author(s):  
K. Dylewska ◽  
G. Sahin ◽  
J. G. Widdicombe
Keyword(s):  
Vagus Nerve ◽  
Vascular Resistance ◽  
Mechanical Stimulation ◽  
Superior Laryngeal Nerve ◽  
Laryngeal Nerve ◽  
Vascular Responses ◽  
The Difference ◽  
Cervical Trachea ◽  
Stimulation Of

Both sides of the nasal vasculature of the dog in vivo were perfused separately, with measurement of vascular resistance responses to stimulation of various nerves. Stimulation of the central end of a cut superior laryngeal nerve caused an ipsilateral vasodilation (-4.98%) and a contralateral vasoconstriction (+3.96%), the difference being statistically significant (P < 0.01). Stimulation of a glossopharyngeal nerve caused vasodilation on both sides, the ipsilateral (-17.52%) being greater than the contralateral (-6.33%) response (P < 0.05). Mechanical stimulation of the nasal mucosa caused little ipsilateral change (+0.47%) and a weak contralateral vasoconstriction (+3.78%; P < 0.01). Stimulation of the central end of a cervical vagus nerve caused vasodilations on both sides, the ipsilateral (-9.75%) being greater than the contralateral (-5.73%) change (P < 0.05). With bilateral perfusions of the cervical tracheal arteries, stimulation of a superior laryngeal nerve caused vasodilation on both sides, the ipsilateral (-10.1%) being greater than the contralateral (-7.4%) response (P < 0.05). Stimulation of the central end of a vagus nerve caused vasoconstrictions on both the sides, the ipsilateral (+37.4%) being greater than the contralateral (+10.8%) change (P < 0.05). Thus various nervous inputs from the nose, pharynx, larynx, and vagal distribution cause asymmetric vascular responses both in the nose and in the cervical trachea.

Echocardiographic assessment of aortic elastic properties with automated border detection in an ICU: in vivo application of the arctangent Langewouters model

2005 ◽  
Vol 288 (5) ◽  
pp. H2504-H2511 ◽  
Author(s):  
Jan R. Heerman ◽  
Patrick Segers ◽  
Carl D. Roosens ◽  
Frank Gasthuys ◽  
Pascal R. Verdonck ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Arterial Compliance ◽  
Ascending Aorta ◽  
Border Detection ◽  
Cross Sectional ◽  
Ultrasound Monitoring ◽  
Total Arterial Compliance ◽  
Pressure Area ◽  
Echocardiographic Assessment

We studied whether combined pressure and transesophageal ultrasound monitoring is feasible in the intensive care unit (ICU) setting for global cardiovascular hemodynamic monitoring [systemic vascular resistance (SVR) and total arterial compliance (CPPM)] and direct estimation of local ascending and descending aortic mechanical properties, i.e., distensibility and compliance coefficients (DC and CC). Pressure-area data were fitted to the arctangent Langewouters model, with aortic cross-sectional area obtained via automated border detection. Data were measured in 19 subjects at baseline, during infusion of sodium nitroprusside (SNP), and after washout. SNP infusion lowered SVR from 1.15 ± 0.40 to 0.80 ± 0.32 mmHg·ml−1·s ( P < 0.05), whereas CPPM increased from 0.87 ± 0.46 to 1.02 ± 0.42 ml/mmHg ( P < 0.05). DC and CC increased from 0.0018 ± 0.0007 to 0.0025 ± 0.0009 l/mmHg ( P < 0.05) and from 0.0066 ± 0.0028 to 0.0083 ± 0.0026 cm2/mmHg ( P < 0.05), respectively, at the descending, but not ascending, aorta. The Langewouters model fitted the descending aorta data reasonably well. Assessment of local mechanical properties of the human ascending aorta in a clinical setting by automated border detection remains technically challenging.

Pressure-flow behavior of a bronchopleural fistula during mechanical ventilation with positive pressure

1989 ◽  
Vol 66 (4) ◽  
pp. 1789-1799 ◽  
Author(s):  
J. J. Perez Fontan ◽  
A. O. Ray
Keyword(s):  
Gas Flow ◽  
Flow Behavior ◽  
Bronchopleural Fistula ◽  
Transmural Pressure ◽  
Positive Pressure ◽  
Inflation Pressure ◽  
Tracheal Pressure ◽  
Flow Through ◽  
The Difference ◽  
The Relationship

We examined the mechanical behavior of a bronchopleural fistula created by sectioning a small subpleural bronchus in seven anesthetized lambs. The pressure across the fistula was measured as the difference between the pressure recorded by a retrograde bronchial catheter inserted in the vicinity of the fistula and the outflow pressure at the fistula exit. The effective resistance of the fistula (Rf) was computed by dividing this pressure difference by the gas flow through the fistula measured at the outlet of an intrapleural tube adjacent to the fistula. Rf increased by 114 +/- 25% (SE) when we inflated the lungs in a stepwise manner from a tracheal pressure of 2–20 cmH2O. Rf also increased when inflation pressure varied continuously; this increase, however, was less evident when we decreased the inflation time from 1.0 to 0.2 s. The relationship between Rf and lung volume was similar during the stepwise inflations and deflations but showed marked hysteresis during the continuous inflation-deflation maneuvers, when Rf was greater during deflation than inflation. Our results suggest that the fistula behaves as a compliant pathway whose relevant transmural pressure is the transmural pressure at or near the fistula's exit. We attribute the increase in Rf during inflation to decreases in transmural pressure caused by convective and dissipative losses inside the fistula and by the stress applied by the chest wall on the outer surface of the fistula.

Hair cell changes after cationic stimulation of corti's organ

1989 ◽  
Vol 47 ◽  
pp. 798-799
Author(s):  
Cesar D. Fermin ◽  
Hans-Peter Zenner
Keyword(s):  
Organ Of Corti ◽  
Cross Sectional ◽  
Surface Areas ◽  
High K ◽  
Anatomical Arrangement ◽  
Cell Cytosol ◽  
High Concentrations ◽  
K Concentration ◽  
Cell Changes

Contraction of outer and inner hair cells (OHC&IHC) in the Organ of Corti (OC) of the inner ear is necessary for sound transduction. Getting at HC in vivo preparations is difficult. Thus, isolated HCs have been used to study OHC properties. Even though viability has been shown in isolated (iOHC) preparations by good responses to current and cationic stimulation, the contribution of adjoining cells can not be explained with iOHC preparations. This study was undertaken to examine changes in the OHC after expossure of the OHC to high concentrations of potassium (K) and sodium (Na), by carefully immersing the OC in either artifical endolymph or perilymph. After K and Na exposure, OCs were fixed with 3% glutaraldehyde, post-fixed in osmium, separated into base, middle and apex and embedded in Araldite™. One μm thick sections were prepared for analysis with the light and E.M. Cross sectional areas were measured with Bioquant™ software.Potassium and sodium both cause isolated guinea pig OHC to contract. In vivo high K concentration may cause uncontrolled and sustained contractions that could contribute to Meniere's disease. The behavior of OHC in the vivo setting might be very different from that of iOHC. We show here changes of the cell cytosol and cisterns caused by K and Na to OHC in situs. The table below shows results from cross sectional area measurements of OHC from OC that were exposed to either K or Na. As one would expect, from the anatomical arrangement of the OC, OHC#l that are supported by rigid tissue would probably be displaced (move) less than those OHC located away from the pillar. Surprisingly, cells in the middle turn of the cochlea changed their surface areas more than those at either end of the cochlea. Moreover, changes in surface area do not seem to differ between K and Na treated OCs.

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