scholarly journals Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method

1992 ◽  
Vol 3 (suppl b) ◽  
pp. 101-105
Author(s):  
Michel Pagé ◽  
Denis Thibeault ◽  
Hélène Tremblay ◽  
Christiane Noël ◽  
Marie-Josée Perron
Keyword(s):  
Cell Lines ◽  
Pharmacological Activity ◽  
Tumour Cell ◽  
Immunological Specificity ◽  
Coupling Procedure ◽  
Novel Method ◽  
Tumour Cell Lines

Most of the reported methods for coupling anthracycline drugs to antibody result in either a loss of pharmacological activity or yield antibodies which have lost much of their immunological specificity. The use of glutaraldehyde for coupling saves both the pharmacological and immunological activities but it causes some polymerization of the antibody or of the macromolecular carrier which is undesirable for in vivo use. A new coupling procedure is reported which uses an activated derivative of daunorubicin added to monoclonal antihuman sarcoma antibody. This coupling procedure has not resulted in significant polymerization of the antibody or Joss of pharmacological activity determined by testing normal and tumour cell Lines in vitro.

scholarly journals Masitinib Combined with Standard Gemcitabine Chemotherapy: In Vitro and In Vivo Studies in Human Pancreatic Tumour Cell Lines and Ectopic Mouse Model

PLoS ONE ◽  
2010 ◽  
Vol 5 (3) ◽  
pp. e9430 ◽  
Author(s):  
Martine Humbert ◽  
Nathalie Castéran ◽  
Sébastien Letard ◽  
Katia Hanssens ◽  
Juan Iovanna ◽  
...  
Keyword(s):  
Mouse Model ◽  
Cell Lines ◽  
Tumour Cell ◽  
In Vivo Studies ◽  
Pancreatic Tumour ◽  
Tumour Cell Lines

scholarly journals Effect of aspirin on tumour cell colony formation and evolution

2017 ◽  
Vol 14 (134) ◽  
pp. 20170374 ◽  
Author(s):  
Dominik Wodarz ◽  
Ajay Goel ◽  
C. Richard Boland ◽  
Natalia L. Komarova
Keyword(s):  
Cell Lines ◽  
Cell Population ◽  
Tumour Cell ◽  
Evolutionary Potential ◽  
A Cell ◽  
Tumour Cell Lines ◽  
Underlying Mechanisms ◽  
Cell Colony

Aspirin is known to reduce the risk of colorectal cancer (CRC) incidence, but the underlying mechanisms are not fully understood. In a previous study, we quantified the in vitro growth kinetics of different CRC tumour cell lines treated with varying doses of aspirin, measuring the rate of cell division and cell death. Here, we use these measured parameters to calculate the chances of successful clonal expansion and to determine the evolutionary potential of the tumour cell lines in the presence and absence of aspirin. The calculations indicate that aspirin increases the probability that a single tumour cell fails to clonally expand. Further, calculations suggest that aspirin increases the evolutionary potential of an expanding tumour cell colony. An aspirin-treated tumour cell population is predicted to result in the accumulation of more mutations (and is thus more virulent and more difficult to treat) than a cell population of the same size that grew without aspirin. This indicates a potential trade-off between delaying the onset of cancer and increasing its evolutionary potential through chemoprevention. Further work needs to investigate to what extent these findings apply to in vivo settings, and to what degree they contribute to the epidemiologically documented aspirin-mediated protection.

Immunophenotype of Mammary Tumour Cell Lines for In-Vitro Analysis Using Three Different Techniques

2020 ◽  
Vol 174 ◽  
pp. 184
Author(s):  
F. Torrigiani ◽  
A. Sammarco ◽  
M.E. Gelain ◽  
F. Bonsembiante ◽  
R. Zanetti ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Mammary Tumour ◽  
Tumour Cell Lines ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Novel O-methyl goniofufurone and 7-epi-goniofufurone derivatives: synthesis, in vitro cytotoxicity and SAR analysis

MedChemComm ◽  
2018 ◽  
Vol 9 (12) ◽  
pp. 2017-2027
Author(s):  
Jovana Francuz ◽  
Mirjana Popsavin ◽  
Sanja Djokić ◽  
Vesna Kojić ◽  
Tatjana Srdić-Rajić ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Methoxy Group ◽  
Antitumour Activity ◽  
In Vitro Cytotoxicity ◽  
Human Tumour ◽  
Tumour Cell Lines ◽  
Sar Analysis ◽  
Human Tumour Cell Lines

Novel goniofufurone (1) and 7-epi-goniofufurone (2) derivatives bearing a methoxy group at the C-5 and/or C-7 positions were prepared and their in vitro antitumour activity against some human tumour cell lines was evaluated.

scholarly journals Synthesis, Characterization and High In Vitro Antitumour Activity of Novel Triphenyltin Carboxylates

1994 ◽  
Vol 1 (4) ◽  
pp. 305-309 ◽  
Author(s):  
Marcel Gielen ◽  
Abdelaziz El Khloufi ◽  
Monique Biesemans ◽  
Abdeslam Bouhdid ◽  
Dick de Vos ◽  
...  
Keyword(s):  
Colon Carcinoma ◽  
Cell Lines ◽  
Tumour Cell ◽  
Antitumour Activity ◽  
Human Tumour ◽  
Tumour Cell Lines ◽  
Human Tumour Cell Lines ◽  
Mcf 7

The synthesis and spectral characterization of six novel triphenyltin compounds are described. The in vitro antitumour activity of three of these compounds against two human tumour cell lines, MCF-7, a mammary tumour, and WiDr, a colon carcinoma, was determined. All three compounds are more active than cis-platin, etoposide and doxorubicin against both tumour cell lines. They are as active as mitomycin C against WiDr, but less active against MCF-7.

In-vitro anti-proliferative effects of some anti-tumour drugs on feline mammary tumour cell lines

1999 ◽  
Vol 66 (3) ◽  
pp. 169-174 ◽  
Author(s):  
J.S MULEYA ◽  
M NAKAICHI ◽  
Y TAURA ◽  
R YAMAGUCHI ◽  
S NAKAMA
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Mammary Tumour ◽  
Tumour Cell Lines

scholarly journals Di-n-Butyl-, Tri-n-Butyl- and Triphenyltin dl-Terebates: Synthesis, Characterization and In Vitro Antitumour Activity

1997 ◽  
Vol 4 (4) ◽  
pp. 193-197 ◽  
Author(s):  
Marcel Gielen ◽  
Huairang Ma ◽  
Abdeslam Bouhdid ◽  
Hassan Dalil ◽  
Monique Biesemans ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Antitumour Activity ◽  
Human Tumour ◽  
Tumour Cell Lines ◽  
Human Tumour Cell Lines

Di-n-butyltin, tri-n-butyltin and triphenyltin terebates were screened against several human tumour cell lines and found comparably or more active than carboplatin, cis-platin, 5-fluorouracil, methotrexate and doxorubicin, some reference compounds used clinically.

l-Asparaginyl-Trna Synthetase and l-Asparagine Synthetase Activities of l-Asparaginase-Sensitive and -Resistant Forms of the Mouse Gardner Lymphoma 6C3HED

1979 ◽  
Vol 56 (6) ◽  
pp. 539-545 ◽  
Author(s):  
M. R. Davies ◽  
Lucy P. Lambert ◽  
R. D. Marshall
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Trna Synthetase ◽  
Asparagine Synthetase ◽  
Ascites Fluid ◽  
Synthetase Activity ◽  
Early Passage ◽  
Tumour Cell Lines ◽  
Cells Cultured

1. The mouse Gardner lymphoma 6C3HED was grown in ascites fluid in a form sensitive to the action of l-asparaginase (line 1), in another form which was resistant to l-asparaginase (line 2) and in a third form with partial sensitivity to l-asparaginase (line 3). 2. The l-asparaginyl-tRNA synthetase activities of extracts of the tumour cells, cultured both in the mouse and in vitro, were determined. Two of the lines, 1 and 3, in early passage numbers, showed a derepression mechanism involving l-asparagine. Mutation occurred with these lines resulting in the l-asparaginyl-tRNA synthetase activity of all the tumour cell lines being the same. 3. Cells of line 1 had low l-asparagine synthetase activity, which was unchanged by altering the supply of l-asparagine in vitro. Cells of lines 2 and 3 exhibited l-asparagine synthetase activities, which changed with the supply of l-asparagine. 4. It is not certain that l-asparagine synthetase activity of l-asparaginase-sensitive cells is controlled by l-asparaginyl-tRNA acting as a corepressor.

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