scholarly journals Origin of Microcells in the Human Sarcoma Cell Line HT-1080

1999 ◽  
Vol 18 (2) ◽  
pp. 73-85 ◽  
Author(s):  
Indulis Buiķis ◽  
Līga Harju ◽  
Tālivaldis Freivalds
Keyword(s):  
Cell Line ◽  
Apoptotic Cell ◽  
Sarcoma Cell ◽  
Electron Microscopic ◽  
Sarcoma Cell Line ◽  
Lung Resistance Protein ◽  
Coiled Bodies ◽  
Lung Resistance ◽  
Resistance Protein ◽  
Human Sarcoma

The aim of this study was to investigate the development of microcells in the human sarcoma cell line HT‐1080 after interference with thiophosphamidum. We found that damaged interphase macrocells located at the projection of the nucleolus may form one or several microcells. The micronuclei of the microcells intensively incorporate the thymidine analogue 5‐bromo‐2'‐deoxyuridine and strongly express argyrophilic nucleolar organiser region proteins. At an early phase of the development, the micronuclei contain fragmented DNA, but in subsequent phases, the micronuclei accumulate polymeric DNA, simultaneously with an increase in their size. After desintegration of the damaged macrocell, the microcells appear in the intercellular space. The microcells can enter mitosis and they strongly express the lung resistance protein. Electron microscopic observations suggest that coiled bodies are involved in the development of the microcells. Since the observed path of microcell formation differs from apoptotic cell fragmentation into apoptotic bodies, we propose a new term for this microcell development:sporosis. We suggest that self‐renewal of the tumour stem cells is likely based on sporosis.

scholarly journals Prognosis and Immune Infiltration of Chromobox Family Genes in Sarcoma

2021 ◽  
Vol 11 ◽  
Author(s):  
Jian Zhou ◽  
Ziyuan Chen ◽  
Ming Zou ◽  
Rongjun Wan ◽  
Tong Wu ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Line ◽  
Prognostic Value ◽  
Disease Free Survival ◽  
Sarcoma Cell ◽  
Free Survival ◽  
Sarcoma Cell Line ◽  
Expression Levels ◽  
Immune Infiltration ◽  
Human Sarcoma

BackgroundChromobox family genes (CBXs) are known to play roles in numerous modifications of the chromatin in order to inhibit the transcription of target genes. CBXs have been shown to be expressed at high levels in many types of cancer and can also serve as a target gene for therapeutic purposes. However, little is known about the expression and prognostic value of CBXs in human sarcomas.MethodsThe transcription level of CBXs was analyzed using the Oncomine dataset, and the differential expression of CBXs in sarcoma was reported by the Gene Expression Profiling Interactive Analysis (GEPIA) dataset. We also used the CCLE dataset to evaluate the expression of CBXs in a sarcoma cell line. The prognostic value of CBXs was analyzed using GEPIA and Kaplan–Meier analysis. In addition, the corrections between CBXs and their co-expressed genes were reported using Oncomine and GEPIA datasets. DAVID was used to perform GO function enrichment analysis for the CBXs and their co-expression genes. Finally, TIMER was used to analyze the immune cell infiltration of CBXs in patients with sarcoma.ResultsHP1-α/β/γ (CBX1/3/5) and CBX4/6/8 were found to be overexpressed in human sarcoma, and CBXs were upregulated in almost all the sarcoma cell line. The expression levels of HP1-α/β/γ (CBX1/3/5) and CBX7 were associated with overall survival (OS) in patients with sarcoma, while high expression levels of CBX7 were related to disease-free survival (DFS). In addition, the expression levels of CBX2/6/7 were related to recurrence-free survival (RFS). We also found that the CBX family was positively correlated with the infiltration of immune cells, including CD8+ T cells, CD4+ T cells, B cells, macrophages, neutrophils, and dendritic cells, in sarcoma.ConclusionsThe results from the present study indicated that CBXs were significantly associated with prognosis and immunological status in sarcoma. These data suggest that CBXs could serve as potential biomarkers for prognosis and immune infiltration in human sarcoma.

Euphorbiasteroid reverses P-glycoprotein-mediated multi-drug resistance in human sarcoma cell line MES-SA/Dx5

2009 ◽  
Vol 24 (7) ◽  
pp. 1042-1046 ◽  
Author(s):  
Jung Sook Choi ◽  
Nam Sook Kang ◽  
Yong Ki Min ◽  
Seong Hwan Kim
Keyword(s):  
Drug Resistance ◽  
Cell Line ◽  
Multi Drug Resistance ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
P Glycoprotein ◽  
Human Sarcoma

scholarly journals Production of cell-associated PDGF-AA by a human sarcoma cell line: evidence for a latent autocrine effect

1996 ◽  
Vol 68 (6) ◽  
pp. 802-809 ◽  
Author(s):  
Mozhgan Afrakhte ◽  
Monica Mistér ◽  
Arne Östman ◽  
Bengt Westermark ◽  
Ylva Paulsson
Keyword(s):  
Cell Line ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Human Sarcoma

scholarly journals Genetic and Epigenetic Modeling of the Origins of Multidrug-Resistant Cells in a Human Sarcoma Cell Line

2005 ◽  
Vol 65 (20) ◽  
pp. 9388-9397 ◽  
Author(s):  
Kevin G. Chen ◽  
Yan C. Wang ◽  
Marci E. Schaner ◽  
Brian Francisco ◽  
George E. Durán ◽  
...  
Keyword(s):  
Cell Line ◽  
Multidrug Resistant ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Human Sarcoma ◽  
Resistant Cells

scholarly journals Apoptotic Cell Death of High Polyploid Cells in a Cultured Sarcoma Cell Line.

1998 ◽  
Vol 23 (4) ◽  
pp. 231-237 ◽  
Author(s):  
Zhi-Ping Zong ◽  
Kohzaburo Fujikawa-Yamamoto ◽  
Takahide Ota ◽  
Manabu Murakami ◽  
Ai-Li Li ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
High Polyploid ◽  
Polyploid Cells

scholarly journals Characterization of a newly derived human sarcoma cell line (HT-1080)

Cancer ◽  
1974 ◽  
Vol 33 (4) ◽  
pp. 1027-1033 ◽  
Author(s):  
Suraiya Rasheed ◽  
Walter A. Nelson-Rees ◽  
Eva M. Toth ◽  
Paul Arnstein ◽  
Murray B. Gardner
Keyword(s):  
Cell Line ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Human Sarcoma

Modulation of glucose transport by parathyroid hormone and insulin in UMR 106–01, a clonal rat osteogenic sarcoma cell line

1995 ◽  
Vol 14 (2) ◽  
pp. 263-275 ◽  
Author(s):  
D M Thomas ◽  
S D Rogers ◽  
M W Sleeman ◽  
G M Pasquini ◽  
F R Bringhurst ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cell Line ◽  
Glucose Transport ◽  
Transport System ◽  
Osteogenic Sarcoma ◽  
Regulatory Subunit ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Glucose Transport System ◽  
Umr 106

ABSTRACT This study characterizes the actions of insulin and parathyroid hormone (PTH) on the glucose transport system in the rat osteogenic sarcoma cell line UMR 106–01, which expresses a number of features of the osteoblast phenotype. Using [1,2-3H]2-deoxyglucose (2-DOG) as a label, UMR 106–01 cells were shown to possess a glucose transport system which was enhanced by insulin. In contrast, PTH influenced glucose transport in a biphasic manner with a stimulatory effect at 1 h and a more potent inhibitory effect at 16 h on basal and insulin-stimulated 2-DOG transport. To explore the mechanism of PTH action, a direct agonist of cAMP-dependent protein kinase (PKA) was tested. 8-Bromo-cAMP had no acute stimulatory effect but inhibited basal and insulin-stimulated 2-DOG transport at 16 h. This result suggested that the prolonged, but not the acute, effect of PTH was mediated by the generation of cAMP. Further studies with the cell line UMR 4–7, a UMR 106–01 clone stably transfected with an inducible mutant inactive regulatory subunit of PKA, confirmed that the inhibitory but not the stimulatory effect of PTH was mediated by the PKA pathway. Northern blot data indicated that the prolonged inhibitory effects of PTH and 8-bromo-cAMP on glucose transport were likely to be mediated in part by reduction in the levels of GLUT1 (HepG2/brain glucose transporter) mRNA.

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