Effect of Treatment of Sprague Dawley Rats with AVE7688, Enalapril, or Candoxatril on Diet-Induced Obesity

The objective of this study was to determine the effect of AVE7688, a drug that inhibits both angiotensin converting enzyme (ACE) and neutral endopeptidase (NEP) activity, on neural and vascular defects caused by diet induced obesity (DIO). Rats at 12 weeks of age were fed a standard or high fat diet with or without AVE7688 for 24 weeks. DIO rats had impaired glucose tolerance and developed sensory neuropathy. Vascular relaxation to acetylcholine and calcitonin gene-related peptide was decreased in epineurial arterioles of DIO rats. Rats fed a high fat diet containing AVE7688 did not become obese and vascular and sensory nerve dysfunction and impaired glucose tolerance were improved. DIO is associated with increased expression of NEP in epineurial arterioles. NEP degrades vasoactive peptides which may explain the decrease in neurovascular function in DIO.

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Protection from high-fat-diet-induced impaired glucose tolerance in female Sprague-Dawley rats

Gynecological Endocrinology ◽

10.1080/09513590902770107 ◽

2009 ◽

Vol 25 (7) ◽

pp. 464-471 ◽

Cited By ~ 6

Author(s):

Zhongyan Lu ◽

Zhe Wang ◽

Xiaodong Wang ◽

Bo Diao ◽

Xinyan Feng ◽

...

Keyword(s):

Glucose Tolerance ◽

Impaired Glucose Tolerance ◽

High Fat Diet ◽

Sprague Dawley ◽

High Fat ◽

Sprague Dawley Rats

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Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats

BioMed Research International ◽

10.1155/2018/5479057 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6 ◽

Cited By ~ 5

Author(s):

O. Merino ◽

R. Sánchez ◽

B. M. Gregorio ◽

F. J. Sampaio ◽

J. Risopatrón

Keyword(s):

Vitamin D ◽

Dna Fragmentation ◽

High Fat Diet ◽

Control Diet ◽

Sperm Function ◽

Sprague Dawley ◽

High Fat ◽

Diet Induced Obesity ◽

Sprague Dawley Rats ◽

The Impact

Obesity has adverse effects on male fertility and usually is diagnosed with a prevalence of vitamin D deficiency (VD-). Discussion on the impact of obesity/VD- on sperm function has been limited. This study analyzed the effects of diet-induced obesity/VD- on viability and plasma membrane integrity (PMI), superoxide anion (O2-) level, and DNA fragmentation (DNAfrag) in sperm Sprague-Dawley rats. The males were randomized into four groups and fed for a period of 12 weeks: G1: control diet with vitamin D (C/VD+), G2: control diet without vitamin D (C/VD-), G3: high-fat diet with vitamin D (HF/VD+), and G4: high-fat diet without vitamin D (HF/VD-). Sperm function parameters were analyzed by flow cytometry. PMI percentages and O2- levels were not affected by any of the diets. DNA fragmentation was increasing significantly (p<0.05) in the spermatozoa of animals with diets vitamin D deficient (G2) and diet-induced obesity (G4). Our results allow us to point out that diet-induced obesity and VD- produce greater damage in DNA sperm of rats. The use of nutraceuticals containing vitamin D could be reducing the risk of fragmentation of DNA in spermatozoa.

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Lactobacillus paracasei subsp. paracasei NTU 101 ameliorates impaired glucose tolerance induced by a high-fat, high-fructose diet in Sprague-Dawley rats

Journal of Functional Foods ◽

10.1016/j.jff.2016.04.033 ◽

2016 ◽

Vol 24 ◽

pp. 472-481 ◽

Cited By ~ 8

Author(s):

Szu-Chi Hung ◽

Wei-Ting Tseng ◽

Tzu-Ming Pan

Keyword(s):

Glucose Tolerance ◽

Impaired Glucose Tolerance ◽

Lactobacillus Paracasei ◽

Sprague Dawley ◽

High Fat ◽

High Fructose Diet ◽

Sprague Dawley Rats ◽

High Fructose

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Atypical cannabinoid ligands O-1602 and O-1918 administered chronically in diet-induced obesity

Endocrine Connections ◽

10.1530/ec-18-0535 ◽

2019 ◽

Vol 8 (3) ◽

pp. 203-216 ◽

Cited By ~ 3

Author(s):

Anna C Simcocks ◽

Kayte A Jenkin ◽

Lannie O’Keefe ◽

Chrishan S Samuel ◽

Michael L Mathai ◽

...

Keyword(s):

Body Weight ◽

G Protein ◽

High Fat Diet ◽

Chow Diet ◽

Sprague Dawley ◽

High Fat ◽

G Protein Coupled Receptor ◽

Diet Induced Obesity ◽

Sprague Dawley Rats ◽

G Protein Coupled

Atypical cannabinoid compounds O-1602 and O-1918 are ligands for the putative cannabinoid receptors G protein-coupled receptor 55 and G protein-coupled receptor 18. The role of O-1602 and O-1918 in attenuating obesity and obesity-related pathologies is unknown. Therefore, we aimed to determine the role that either compound had on body weight and body composition, renal and hepatic function in diet-induced obesity. Male Sprague–Dawley rats were fed a high-fat diet (40% digestible energy from lipids) or a standard chow diet for 10 weeks. In a separate cohort, male Sprague–Dawley rats were fed a high-fat diet for 9 weeks and then injected daily with 5 mg/kg O-1602, 1 mg/kg O-1918 or vehicle (0.9% saline/0.75% Tween 80) for a further 6 weeks. Our data demonstrated that high-fat feeding upregulates whole kidney G protein receptor 55 expression. In diet-induced obesity, we also demonstrated O-1602 reduces body weight, body fat and improves albuminuria. Despite this, treatment with O-1602 resulted in gross morphological changes in the liver and kidney. Treatment with O-1918 improved albuminuria, but did not alter body weight or fat composition. In addition, treatment with O-1918 also upregulated circulation of pro-inflammatory cytokines including IL-1α, IL-2, IL-17α, IL-18 and RANTES as well as plasma AST. Thus O-1602 and O-1918 appear not to be suitable treatments for obesity and related comorbidities, due to their effects on organ morphology and pro-inflammatory signaling in obesity.

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The Effects of Duodenojejunal Omega Switch in Combination with High-Fat Diet and Control Diet on Incretins, Body Weight, and Glucose Tolerance in Sprague-Dawley Rats

Obesity Surgery ◽

10.1007/s11695-017-2883-3 ◽

2017 ◽

Vol 28 (3) ◽

pp. 748-759 ◽

Cited By ~ 10

Author(s):

Dominika Stygar ◽

Tomasz Sawczyn ◽

Bronisława Skrzep-Poloczek ◽

Aleksander J. Owczarek ◽

Natalia Matysiak ◽

...

Keyword(s):

Body Weight ◽

Glucose Tolerance ◽

High Fat Diet ◽

Control Diet ◽

Sprague Dawley ◽

High Fat ◽

Sprague Dawley Rats ◽

And Control

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The influence of high fat diet on plasma incretins and insulin concentrations in Sprague-Dawley rats with diet-induced obesity and glucose intolerance undergoing ileal transposition

Peptides ◽

10.1016/j.peptides.2019.04.001 ◽

2019 ◽

Vol 115 ◽

pp. 75-84

Author(s):

Tomasz Sawczyn ◽

Dominika Stygar ◽

Katarzyna Nabrdalik ◽

Michał Kukla ◽

Bronisława Skrzep-Poloczek ◽

...

Keyword(s):

Glucose Intolerance ◽

High Fat Diet ◽

Ileal Transposition ◽

Sprague Dawley ◽

High Fat ◽

Diet Induced Obesity ◽

Sprague Dawley Rats

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Potential of Hibiscus sabdariffa linn. in managing Fgf21 resistance in diet-induced-obesity rats via miR-34a regulation

10.21203/rs.3.rs-112182/v1 ◽

2020 ◽

Author(s):

Neng Tine Kartinah ◽

Nisa Komara ◽

Nuraini Diah Noviati ◽

Syarifah Dewi ◽

Sophie Yolanda ◽

...

Keyword(s):

Adipose Tissue ◽

High Fat Diet ◽

Receptor Expression ◽

Hibiscus Sabdariffa ◽

Sprague Dawley ◽

High Fat ◽

Diet Induced Obesity ◽

Sprague Dawley Rats ◽

Equivalent Expression ◽

Potential Activity

Abstract Background Obesity is a cause of Fgf21 resistance, which affects the browning and thermogenesis process of the adipose tissue. Decreased receptor expression is influenced by microRNA 34a (miR-34a), whose expression is increased in obesity. While Fgf21-based therapies have been widely investigated, the potential activity of Hibiscus sabdariffa Linn extract (HSE) against Fgf21 resistance is unknown. This study aims to determine the effects of HSE on the expression of miR-34a and Fgf21 receptors in white adipose tissue. Methods This experimental study used 24 male Sprague-Dawley rats and divided into four groups: Control (N); diet-induced-obesity rats (DIO); DIO rats with HSE 200 mg/kgBW/day and DIO rats with HSE 400 mg/kgBW/day. Rats were fed a high-fat diet for 17 weeks. HSE was administered daily for five weeks. The administration of HSE 400 mg/kg BW/day resulted in the equivalent expression of miR-34a to that of the control (p > 0.05). Results Fgfr1 receptor expression was also similar to controls (p > 0.05). Beta-klotho expression was significantly lower than that of Control (p < 0.05) but equivalent to that of DIO rats (p < 0.05). Conclusions H. sabdariffa has the potential to reduce Fgf21 resistance in DIO rats through the suppression of miR-34a expression and an increase in the number of Fgfr1 and beta-klotho receptors in adipose tissue.

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