scholarly journals Circulating microRNAs and Kallikreins before and after Radical Prostatectomy: Are They Really Prostate Cancer Markers?

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Maria Giulia Egidi ◽  
Giovanni Cochetti ◽  
Maria Rita Serva ◽  
Gabriella Guelfi ◽  
Danilo Zampini ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Radical Prostatectomy ◽  
Real Time Pcr ◽  
Serum Levels ◽  
Cancer Markers ◽  
Circulating Micrornas ◽  
Before And After ◽  
Inflammatory Processes ◽  
Postoperative Serum ◽  
Circulating Levels

The aim of our study was to monitor serum levels of two miRNAs (miR-21 and miR-141) and three KLKs (hK3/PSA, hK11, and hK13) before and 1, 5, and 30 days after radical prostatectomy, in order to characterize their fluctuations after surgery. 38 patients with prostate cancer were included. miR-21 and miR-141 were quantified through real-time PCR, while ELISA assays were used to quantify hK3 (PSA), hK11, and hK13. Both miR-21 and miR-141 showed a significant increase at the 5th postoperative day, after which a gradual return to the preoperative levels was recorded. These findings suggest that miR-21 and miR-141 could be involved in postsurgical inflammatory processes and that radical prostatectomy does not seem to alter their circulating levels. Postoperative serum kallikreins showed a significant decrease, highlighting the potential usefulness of kallikreins apart from PSA as potential prostate cancer markers.

Hypergonadotrophic hypogonadism in an XX female subject due to 17,20 steroid desmolase deficiency

1983 ◽  
Vol 103 (3) ◽  
pp. 400-405 ◽  
Author(s):  
Fernando Larrea ◽  
Rubén Lisker ◽  
Rosario Bañuelos ◽  
José A. Bermúdez ◽  
Joaquín Herrera ◽  
...  
Keyword(s):  
Autosomal Recessive ◽  
Elevated Serum ◽  
Serum Levels ◽  
Primary Amenorrhoea ◽  
Serum Lh ◽  
Before And After ◽  
Early Follicular Phase ◽  
Normal Women ◽  
Circulating Levels ◽  
Autosomal Recessive Pattern

Abstract. A 22 year old XX female patient with primary amenorrhoea and sexual infantilism was studied. Persistently elevated serum LH and FSH concentrations and exaggerated LRH pituitary responsiveness indicated deficient ovarian hormonal production. Serum levels of C21 and C19 steroids measured by specific radioimmunoassays before and after appropriate stimulations demonstrated an impairment of adrenal and ovarian steroid biosynthesis. Baseline levels of androstenedione (Δ4-A), testosterone (T), and oestradiol-17β (E2) were persistently below the normal range for healthy women at early follicular phase, whereas progesterone (P) and 17α-OH-progesterone (17-OH-P) serum levels were significantly higher than those observed for normal women. Adrenal and gonadal stimulation with ATCH and hCG, respectively, resulted in a considerable rise in serum P and 17-OH-P without any significant change in circulating levels of Δ4-A, T, and E2. These findings were consistent with the diagnosis of 17,20 steroid desmolase deficiency at both adrenal and ovarian levels. This is the first report of a 17,20 desmolase deficiency in an XX individual, and is in line with previous suggestions that familial occurrence of the disorder would fit an autosomal recessive pattern of inheritance.

Patterns of referral for adjuvant radiotherapy after radical prostatectomy in men with prostate cancer: A population-based analysis.

2012 ◽  
Vol 30 (5_suppl) ◽  
pp. 140-140
Author(s):  
Harvey Quon ◽  
Derek Suderman ◽  
Kimi Guilbert ◽  
Pascal Lambert
Keyword(s):  
Prostate Cancer ◽  
High Risk ◽  
Radical Prostatectomy ◽  
Gleason Score ◽  
Odds Ratio ◽  
Adjuvant Radiotherapy ◽  
Randomized Trials ◽  
Radiation Oncologist ◽  
Before And After ◽  
Margin Positive

140 Background: Randomized trials have shown improved biochemical disease free survival after adjuvant radiotherapy (ART) in patients with pT3 or margin positive disease after radical prostatectomy for prostate cancer. This study examines the rates of referral to a radiation oncologist for patients with high risk pathologic features after prostatectomy. Also, the impact of the presentation of these randomized trials will be examined. Methods: All men diagnosed in the province of Manitoba with prostate adenocarcinoma between 2003 and 2008 who underwent radical prostatectomy were identified through a central cancer registry database. Manual chart review was performed and detailed demographic and clinico-pathologic data were analyzed to determine their influence on referral to a radiation oncologist within 6 months of surgery. Analysis of referral rates before and after the presentation of 2 randomized trials were also examined. Results: A total of 1080 patient records of men undergoing prostatectomy for prostate cancer were reviewed. Of these, 546 (50.6%) men had at least one high risk pathologic feature. This includes pT2 margin positive disease in 298/546 (54.6%), pT3a in 154/546 (28.2%), and pT3b in 94/546 (17.2%). Multivariable logistic regression was performed adjusting for age, distance from cancer centre, Gleason score, T stage, perineural invasion, and margin status. Gleason score 8-10 (p<0.0001), higher pathologic T stage (p<0.0001), and farther distance (p=0.0028) were associated with referral for ART. Age and margin status were not significantly associated. Men with pT3a (odds ratio 3.35) and pT3b disease (odds ratio 5.32) were more likely to be referred than pT2 margin positive disease (p<0.0001). There were 78/546 (14.3%) patients with a high risk factor who were referred for ART within 6 months of surgery. The rates of referral were not significantly different before and after the presentation of randomized trials (p=0.60). Conclusions: Men with higher pathologic stage (pT3) and grade (Gleason 8-10) are more likely to receive ART. However, referral for ART did not increase significantly after presentation of the randomized trials and remains underutilized.

A signature of miRNAs in the blood to help prognosticate prostate cancer at the time of diagnosis.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e16558-e16558
Author(s):  
Ailsa Roberg Sita-Lumsden ◽  
Damien Leach ◽  
Andrea Zivi ◽  
Mathias Winkler ◽  
Jonathan Waxman ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Survival Rates ◽  
Benign Prostatic Hypertrophy ◽  
Serum Levels ◽  
The Cancer Genome Atlas ◽  
Circulating Mirnas ◽  
P Values ◽  
Circulating Micrornas ◽  
Cancer Genome Atlas ◽  
Clinically Significant

e16558 Background: Prostate cancer (PCa) is the most commonly diagnosed cancer in men. Around 80% of PCas are diagnosed as early, localised stage yet a subset of these will metastasise and eventually prove fatal. Management decisions are based on risk stratification systems. However, these systems are not able to clearly distinguish indolent from aggressive PCa’s and as a result many patients with indolent cancers may be over treated. Circulating microRNAs (miRNA) may be an easily accessible, suitable biomarker to distinguish true indolent from clinically significant early PCas thus reducing overtreatments. Methods: Blood samples from 24 men with benign prostatic hypertrophy (BPH, n = 8), localised PCa (n = 8) or metastatic PCa (n = 8) were collected at time of diagnosis. All men had intact prostates and were naïve to any endocrine or other cancer therapy. A platform of circulating miRNAs were analysed in serum using Abcam FireflyTM technology. Data collected were independently verified using real-time qPCR (Exiqon TM ). The miRNAs identified as being significantly different between groups were then analysed in a published dataset. Results: Serum levels of seven of the miRs examined were significantly different in patients with prostate cancer compared to control across both platforms (miR-10b, miR-125b, miR-210, miR-21, miR-378a, miR-483 and miR93 all with P values < 0.005). A further four miRNAs could differentiate between the benign and metastatic cohorts (miR-126 P = 0.008, miR-150 P = 0.05, miR375 P = 0.007). Kaplein-Meier analysis further identified that the serum levels of four miRNAs showed significant association with survival rates (miR-21 P = 0.032, miR-126 P = 0.032, miR-150 P = 0.032, miR-93 P = 0.019). On examination in a cohort of 280 men from The Cancer Genome Atlas (TCGA), four miRs from the cohort had significantly different expression in patients who eventually relapsed (miR-21 P = 0.048, miR-375 P = 0.021, miR-210 P = 0.0003, miR-93 P = 0.008) Conclusions: Our circulating miRNA based signature could be used to stratify men at prostate cancer diagnosis and help identify those who are likely to harbour micro metastases and would benefit more from an early radical treatment. The data is being validated in larger cohorts.

Antagonism of serum tri-iodothyronine changes after injections of prolactin in the domestic fowl before and after hatching

1983 ◽  
Vol 99 (3) ◽  
pp. 401-407 ◽  
Author(s):  
E. R. Kühn ◽  
E. Decuypere ◽  
K. Hemschoote ◽  
L. Berghman ◽  
J. Paulussen
Keyword(s):  
Thyroid Gland ◽  
Rhode Island ◽  
Domestic Fowl ◽  
Chronic Administration ◽  
Serum Levels ◽  
Serum Concentrations ◽  
Before And After ◽  
Thyroid Metabolism ◽  
Ovine Prolactin ◽  
Circulating Levels

The influence of an intravenous injection of ovine prolactin on circulating levels of thyroid hormones was studied in Rhode Island Red embryos and chicks after hatching. In the chick embryo, 2 h after injection of 0·1 μg prolactin (on incubation day 19), serum tri-iodothyronine (T3) increased threefold; after 10 or 100 μg prolactin (on incubation day 18) serum T3 increased 15- to 25-fold. These profound increases were not observed in chicks after hatching. Serum concentrations and thyroid content of thyroxine (T4) in embryos and chicks of all ages studied were not influenced by the prolactin injections. Maximal serum concentrations of reverse T3 (rT3) were found on incubation day 18 (110·25 ± 23·36 pmol/l; 71·66 ± 15·18 pg/ml; n = 8), whereas after hatching no rT3 could be detected. An injection of 10 μg prolactin on day 18 depressed serum rT3 after 2 h to 5·68 ± 3·20 pmol/l (3·69 ± 2·08 pg/ml; n = 8; P<0·001); the effect of 100 μg prolactin was less pronounced. After hatching, chronic administration of prolactin resulted in decreased serum levels of T3, but not of T4, and hypertrophy of the follicles in the thyroid gland. It is concluded that prolactin plays a major role in the maturation of embryonic thyroid metabolism by changing the T4-5-monodeiodination into a T4-5′-monodeiodination. The hypertrophy of the thyroid gland observed after hatching following prolonged prolactin administration may be due to decreased negative feedback of T3 on the hypophysis.

Familial prostate cancer cases before and after radical prostatectomy do not show any aggressiveness compared with sporadic cases

Urology ◽  
2003 ◽  
Vol 61 (6) ◽  
pp. 1193-1197 ◽  
Author(s):  
Abdel-Rahmene Azzouzi ◽  
Antoine Valeri ◽  
Luc Cormier ◽  
Georges Fournier ◽  
Philippe Mangin ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Radical Prostatectomy ◽  
Before And After ◽  
Sporadic Cases
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