scholarly journals Variations in the Levels of Mulberroside A, Oxyresveratrol, and Resveratrol in Mulberries in Different Seasons and during Growth

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jin Zhou ◽  
Shun-xiang Li ◽  
Wei Wang ◽  
Xiao-yi Guo ◽  
Xiang-yang Lu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Morus Alba ◽  
The Other ◽  
Different Seasons ◽  
Mulberroside A

This study aimed to investigate the composition of three major stilbenes (mulberroside A, oxyresveratrol, and resveratrol) in different portions of mulberries collected in different seasons and their change molds during growth by high-performance liquid chromatography. Mulberroside A levels were the highest in the bark and roots ofMorus atropurpureaRoxb,Morus albaLinn, andMorus latifoliaPoir. Oxyresveratrol levels were the highest in roots and stem. Both of these high levels were in September. The amount of resveratrol was very low in all samples. In the stem,Morus latifoliaPoir contained more mulberroside A than the other two mulberries. Mulberroside A was not detected in the leaves of the three mulberries. InMorus atropurpureaRoxb seedlings, the root tended to contain more of the three stilbenes than leaves. The temporal peaks of resveratrol were always ahead of those for oxyresveratrol. The levels of the stilbenes varied in different portions of the varieties of mulberries collected in different season and in the seedlings ofMorus atropurpureaRoxb.

scholarly journals EFFECT OF USING L-ASPARAGINASE IN REDUCING ACRYLAMIDE PERCENTAGE IN BISICUT

2016 ◽  
Vol 47 (4) ◽  
Author(s):  
Jebur & et al.
Keyword(s):  
High Performance Liquid Chromatography ◽  
Enzyme Activity ◽  
Liquid Chromatography ◽  
High Performance ◽  
Specific Activity ◽  
Control Sample ◽  
The Other ◽  
Enzyme Efficiency ◽  
Negative Effect ◽  
The Stability

This study was aimed to know the efficiency of partially purified L- asparaginase produced from local isolate from Erwinia spp. to reduce the percentage of acrylamide formed in Biscuit. Four types of biscuit from wheat flour were prepared (T1, T2, T3, T4),and T1 as control. High performance liquid chromatography technique was used to estimate acrylamide ratio in biscuit , Effect of enzyme addition  on flour chemical and rheological properties was studied, also dough behavior ,gluten percentage, water absorption and amylase enzyme activity was estimated. The results revealed  that  the  addition of  experimental asparaginase ( specific activity 20.5 unite mg-1 ) with 1% of flour weight lead to reduce in acrylamide formation in Biscuit  to 89 %  compared  to  control sample ( in absence of enzyme ) . Moreover, the addition of Asparagine to flour at 0.1 % of its weight, where L- asparaginase was available caused a negative effect on enzyme efficiency in reducing the acrylamide in biscuit. So the level of acrylamide was reduced to 57.7 %. In the other hand , the percentage of acryl amide in biscuit was increased to   233 % when the asparagine was added to mixture in absence of L- asparaginase .Addition of  the enzyme to flour have no effect on the percentage value of gluten but improved the  stability of dough .The  enzyme  addition also led to increase amylases activities.  Addition of experimental enzyme had no effect on quality and sensory evaluation of biscuit.

ANTHOCYANIN, AGLYCONE AND AGLYCONE-SUGAR CONTENT OF FRUITS OF TEMPERATE NORTH AMERICAN SPECIES OF Gaylussacia (ERICACEAE)

1988 ◽  
Vol 68 (1) ◽  
pp. 247-253 ◽  
Author(s):  
J. R. BALLINGTON ◽  
W. E. BALLINGER ◽  
E. P. MANESS
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
North American ◽  
High Performance ◽  
Hplc Analysis ◽  
Sugar Content ◽  
North American Species ◽  
The Other ◽  
Phylogenetic Implications

HPLC analysis of the true huckleberry species Gaylussacia baccata, G. dumosa, G. frondosa, G. mosieri, and G. ursina identified the 3-monoarabinosides, 3-monogalactosides, and 3-monoglucosides of cyanidin, delphinidin, malvidin, peonidin, and petunidin. Gaylussacia brachycera contained all anthocyanins, except peonidin-3-arabinoside. Gaylussacia brachycera differed from other species in percent delphinidin-3-arabinoside. It was higher than the other species in percent of the aglycone delphinidin and lower in cyanidin, and also higher in percent of the sugar arabinose. There were no detectable differences among the other species for anthocyanins, aglycones, or aglycone-sugars. The phylogenetic implications of the similarities among species of Gaylussacia and Vaccinium in anthocyanins, aglycones, and aglycone-sugars of the fruit were discussed.Key words: High-performance liquid chromatography, huckleberries, blueberries, chemotaxonomy, taxonomy, biosystematics

Isolation and identification of tuliposides D and F from tulip cultivars

2020 ◽  
Vol 75 (1-2) ◽  
pp. 7-12 ◽  
Author(s):  
Taiji Nomura ◽  
Shinjiro Ogita ◽  
Yasuo Kato
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
High Performance ◽  
The Other ◽  
Isolation And Identification ◽  
Tulipa Gesneriana ◽  
Tissue Extracts ◽  
Spectroscopic Analyses ◽  
Hplc Peak

Abstract6-Tuliposides A (6-PosA) and B (6-PosB) are major defensive secondary metabolites in tulip cultivars (Tulipa gesneriana), having an acyl group at the C-6 position of d-glucose. Although some wild tulip species produce 1,6-diacyl-glucose type of Pos (PosD and PosF), as well as 6-PosA/B, they have not yet been isolated from tulip cultivars. Here, aiming at verifying the presence of PosD and PosF in tulip cultivars, tissue extracts of 25 cultivars were analyzed by high-performance liquid chromatography (HPLC). Although no HPLC peaks for PosD nor PosF were detected in most cultivars, we found two cultivars giving a minute HPLC peak for PosD and the other two cultivars giving that for PosF. PosD and PosF were then purified from petals of cultivar ‘Orca’ and from pistils of cultivar ‘Murasakizuisho’, respectively, and their identities were verified by spectroscopic analyses. This is the first report that substantiates the presence of 1,6-diacyl-glucose type of Pos in tulip cultivars.

scholarly journals Quantitative Comparison of the Marker Compounds in Different Medicinal Parts of Morus alba L. Using High-Performance Liquid Chromatography-Diode Array Detector with Chemometric Analysis

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5592
Author(s):  
Jung-Hoon Kim ◽  
Eui-Jeong Doh ◽  
Guemsan Lee
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Principal Component ◽  
Chemometric Analysis ◽  
Morus Alba ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Marker Compounds

It is thought that the therapeutic efficacy of Morus alba L. is determined by its biological compounds. We investigated the chemical differences in the medicinal parts of M. alba by analyzing a total of 57 samples (15 root barks, 11 twigs, 12 fruits, and 19 leaves). Twelve marker compounds, including seven flavonoids, two stilbenoids, two phenolic acids, and a coumarin, were quantitatively analyzed using a high-performance liquid chromatography-diode array detector and chemometric analyses (principal component and heatmap analysis). The results demonstrated that the levels and compositions of the marker compounds varied in each medicinal part. The leaves contained higher levels of six compounds, the root barks contained higher levels of four compounds, and the twigs contained higher levels of two compounds. The results of chemometric analysis showed clustering of the samples according to the medicinal part, with the marker compounds strongly associated with each part: mulberroside A, taxifolin, kuwanon G, and morusin for the root barks; 4-hydroxycinnamic acid and oxyresveratrol for the twigs and skimmin; chlorogenic acid, rutin, isoquercitrin, astragalin, and quercitrin for the leaves. Our approach plays a fundamental role in the quality evaluation and further understanding of biological actions of herbal medicines derived from various medicinal plant parts.

scholarly journals Identificatin and quantiative analysis of amyrins in Humulus lupulus L.

2014 ◽  
Vol 27 (3) ◽  
pp. 142-144 ◽  
Author(s):  
Kamila Rokicka ◽  
Magdalena Wojciak-Kosior
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Plant Material ◽  
Mobile Phase ◽  
High Performance ◽  
Humulus Lupulus ◽  
The Other ◽  
Humulus Lupulus L ◽  
Validation Parameters ◽  
Quantiative Analysis

ABSTRACT In the paper, the identification and quantification of α- and β-amyrin in five varieties of Humulus lupulus is described. The plant samples were extracted with ethyl acetate and analyzed using of high performance liquid chromatography (HPLC) with PAD detection. The separation was achieved on RP 18 column, at 2 mL/min flow rate and at temperature of 35°C. Acetonitrile was used as a mobile phase. The established calibration curves and the other validation parameters: linearity (correlation coefficient r > 0.9988) and precision (RSD values ranged from 0.14 to 1.81%) were found to be satisfactory for the proposed method. The content of α- and β-amyrin strongly depended on varieties of H. lupulus and amounted from 72.7 to 232.5 μg/g and from 77.9 to 176.9 μg/g of dry plant material, respectively.

scholarly journals Simultaneous Quantification of Two Flavonoids in Morus alba by High Performance Liquid Chromatography Coupled with Photodiode Array Detector

2018 ◽  
Vol 13 (11) ◽  
pp. 1934578X1801301
Author(s):  
Chang-Seob Seo ◽  
Hyeun-Kyoo Shin
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Morus Alba ◽  
Array Detector ◽  
Root Bark ◽  
Relative Standard ◽  
Photodiode Array

The root bark of Morus alba L. (Family: Moraceae) is an important medicinal herb in many countries and has long been used as a traditional medicine for the treatment of cough, fever, blood pressure reduction, and respiratory diseases. In the present study, the simultaneous determination of two flavonoids, kuwanon G and morusin, for quality control of M alba was conducted using high-performance liquid chromatography (HPLC) equipped with photodiode array (PDA) detector. The column used for separation of kuwanon G and morusin was a Gemini C18 analytical column maintained at 45°C. The mobile phase for efficient separation of two analytes was flowed 0.1% (v/v) aqueous formic acid-acetonitrile with gradient elution. The detection wavelength for quantification was set at 266 nm. The optimized method showed good linearity with coefficients of determination of 0.9998 within the tested concentration ranges. The limits of detection for the two flavonoids, kuwanon G and morusin, were 0.69 μg/mL and 0.35 μg/mL and the limits of quantification of kuwanon G and morusin, were 2.10 μ/mL and 1.07 μg/mL. The recoveries were 98.40–111.55% and the relative standard deviation (RSD) value was within 3.50%. The RSD values of intra- a g d interday precisions were 0.08–0.70% and 0.06-0.48%, respectively. The amounts of kuwanon G and morusin were 1.94-2.26 mg/g and 1.05–1.12 mg/g. The established HPLC-PDA method will help to improve the quality control of M. alba and related products.

Achievement of anthocyanins production in grape leaves of Carignan (Vitis vinifera L.) exits of one eye cuttings cultivated in laboratory

OENO One ◽  
1999 ◽  
Vol 33 (1) ◽  
pp. 9
Author(s):  
Béchir Ezzili ◽  
Gérard Darné ◽  
M. Bejaoui
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Leaf Surface ◽  
Leaf Growth ◽  
The Other ◽  
Total Darkness ◽  
Free Form ◽  
Total Anthocyanin ◽  
Anthocyanin Pigments

<p style="text-align: justify;">Under some laboratory conditions, it is possible to obtain a production of anthocyanins by the leaves of grape cuttings.</p><p style="text-align: justify;">The objective of this work consists in the comparison between contents in anthocyanin pigments of leaves of stemming Carignan of one eye cuttings cultivated in various conditions of laboratory with those of leaves of the same cultivated grape to the vineyard in the area of El Khenguet UCP Sidi Slama (Tunisia).</p><p style="text-align: justify;">We have measured the growth in length and in diameter of stems of the cuttings processed 30 days in total darkness and compared to cuttings witnesses raised in greenhouse. The same comparison has been undertaken on cuttings having undergone 30 days stay in total darkness and 21 days of maintenance in greenhouse with witness that has undergone 51 days in greenhouse.</p><p style="text-align: justify;">The total darkness during 30 days favors the growth in length and in diameter of shoots, reduced the leaf surface, and blocks the synthesis of anthocyanin as compared to the photoperiod of 12 hours of darkness/12 hours of light. The total darkness during 30 steady days by a photoperiod of 12 hours light/12 hours darkness during 21 days induces a resumption of the leaf growth and a synthesis of anthocyanins in leaves and in stems.</p><p style="text-align: justify;">The analysis of the anthocyanin, undertaken by High Performance Liquid Chromatography (HPLC) allowed to detect the five anthocyanin 3-monoglucosides following: Delphinidin MG3 - Cyanidin MG3 - Petunidin MG3 - Pæonidin MG3 and Malvidin MG3.</p><p style="text-align: justify;">They are in the free form and in the combined form, esterified by acetic, cafeic and coumaric acids in stemming leaves of the vineyard as well as in those developed in the laboratory. The combined anthocyanins are better represented in the cutting cultivated in laboratory.</p><p style="text-align: justify;">The Cyanidin 3-monoglucoside, the Pæonidin 3-monoglucoside and the Malvidin 3-monoglucoside present a maximal content at the period of the fall of leaves. The other anthocyanin pigments have similar contents always weaker than those of Cyanidin MG3 and Pæonidine MG3.</p><p style="text-align: justify;">The output in total anthocyanin obtained of the cuttings of the laboratory is equal to 25 p. cent of that of the vineyard.</p>

scholarly journals Detection of cheese whey in raw milk preserved with bronopol® through high performance liquid chromatography

2011 ◽  
Vol 63 (6) ◽  
pp. 1553-1558 ◽  
Author(s):  
M.M. Lasmar ◽  
M.O. Leite ◽  
L.M. Fonseca ◽  
M.R. Souza ◽  
M.M.O.P. Cerqueira ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Cheese Whey ◽  
Raw Milk ◽  
The Other ◽  
Random Design ◽  
Non Parametric

High performance liquid chromatography was used in order to detect cheese whey in samples of raw milk preserved with Bronopol®. Six samples were collected and divided in 45 aliquots of 40mL. From these, 15 were used as control and stored frozen, 15 were added with Bronopol® and stored at 7ºC, and the other 15 were added with Bronopol® and stored at 30ºC. In all groups, five levels of cheese whey addition (0, 2, 5, 10, and 20%) were tested. The samples were submitted to high performance liquid chromatography on the 2nd, 4th, and 8th days of storage. A completely random design was used, following the factorial scheme (5x3x3) and the results were compared through the non-parametric Kruskal-Wallis test. There was no difference among the treatments (P>0.05), which allows the conclusion that raw milk preserved with Bronopol® may be used for the determination of cheese whey addition in milk through high performance liquid chromatography.

Ovarian and circulating levels of oxytocin and arginine vasopressin during the estrous cycle in the rat

1992 ◽  
Vol 126 (6) ◽  
pp. 530-534 ◽  
Author(s):  
Mei-Ling Ho ◽  
Jau-Nan Lee
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Estrous Cycle ◽  
Arginine Vasopressin ◽  
Synthetic Peptides ◽  
High Performance ◽  
The Other ◽  
Significant Difference ◽  
Long Evans ◽  
Circulating Levels

Ovarian extracts of Long-Evans rats separated using high performance liquid chromatography (HPLC) were measured by radioimmunoassays (RIAs) for the presence of oxytocin and arginine vasopressin (AVP). The results showed that the ovary contains both, and that they are indistinguishable from the respective standard synthetic peptides. During the estrous cycle, the ovarian content of oxytocin was 10-fold higher (p<0.01) in estrus than in the other phases, while AVP was 16- and 25-fold higher (p<0.01) in metestrus than in the other phases. In contrast, the plasma levels of oxytocin showed no significant difference among the various phases of the estrous cycle. However, the plasma level of AVP level was significantly higher (p<0.01) in diestrus than in other phases. The present study thus strongly supports the hypothesis that both oxytocin and AVP can be produced by the ovary itself in the rat. The possible roles of oxytocin and AVP in the reproductive cycle are discussed.

Sign in / Sign up

Export Citation Format

Share Document