scholarly journals Optical Properties andIn VitroBiological Studies of Oligonucleotide-Modified Quantum Dots

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Valérie A. Gérard ◽  
Mark Freeley ◽  
Eric Defrancq ◽  
Anatoly V. Fedorov ◽  
Yurii K. Gun’ko

Water-soluble semiconducting nanocrystals or quantum dots (QDs) have attracted much interest in recent years due to their tuneable emission and potential applications in photonics and biological imaging. Fluorescence resonance energy transfer (FRET) processes are very important for elucidating biochemical mechanismsin vitro, and QDs constitute an excellent substrate for this purpose. In this work, new oligonucleotide-functionalised CdTe-based QDs were prepared, characterised and biologically tested. These QDs demonstrated interesting optical properties as well as remarkablein vitrobehaviour and potential for a range of biological applications.

2020 ◽  
Vol 39 (1) ◽  
pp. 209-221
Author(s):  
Jiafeng Wan ◽  
Xiaoyuan Zhang ◽  
Kai Zhang ◽  
Zhiqiang Su

Abstract In recent years, nanomaterials have attracted lots of attention from researchers due to their unique properties. Nanometer fluorescent materials, such as organic dyes, semiconductor quantum dots (QDs), metal nano-clusters (MNCs), carbon dots (CDs), etc., are widely used in biological imaging due to their high sensitivity, short response time, and excellent accuracy. Nanometer fluorescent probes can not only perform in vitro imaging of organisms but also achieve in vivo imaging. This provides medical staff with great convenience in cancer treatment. Combined with contemporary medical methods, faster and more effective treatment of cancer is achievable. This article explains the response mechanism of three-nanometer fluorescent probes: the principle of induced electron transfer (PET), the principle of fluorescence resonance energy transfer (FRET), and the principle of intramolecular charge transfer (ICT), showing the semiconductor QDs, precious MNCs, and CDs. The excellent performance of the three kinds of nano fluorescent materials in biological imaging is highlighted, and the application of these three kinds of nano fluorescent probes in targeted biological imaging is also introduced. Nanometer fluorescent materials will show their significance in the field of biomedicine.


2017 ◽  
Vol 19 (2) ◽  
Author(s):  
Anne S. Schulze ◽  
Isabella Tavernaro ◽  
Friederike Machka ◽  
Olga Dakischew ◽  
Katrin S. Lips ◽  
...  

2004 ◽  
Vol 03 (03) ◽  
pp. 273-280
Author(s):  
QI-DAN CHEN ◽  
ZHANG-BI LIN ◽  
XING-GUANG SU ◽  
HAO ZHANG ◽  
XIAO-HONG HE ◽  
...  

3-Mercaptopropyl acid-capped quantum dots (QDs) synthesized in aqueous solution were coupled to avidin-sulforhodamine, also named avidin-Texas red (ATR), via electrostatic attraction. An intensity reduction in the fluorescence emission spectrum of QDs and an enhanced fluorescence intensity of the dye were observed on account of fluorescence resonance energy transfer from the QD donors to the dye acceptors. In addition, the fluorescence characteristics of the QD-ATR conjugates were strongly-related to the quantity of ATR, pH value and ionic strength.


Author(s):  
Viet Ha Chu ◽  
Thi Ha Lien Nghiem ◽  
Tien Ha Le ◽  
Dinh Lam Vu ◽  
Hong Nhung Tran ◽  
...  

2017 ◽  
Vol 8 (5) ◽  
pp. 3879-3884 ◽  
Author(s):  
Gayathri Devatha ◽  
Soumendu Roy ◽  
Anish Rao ◽  
Abhik Mallick ◽  
Sudipta Basu ◽  
...  

InP QDs join the family of cationic nanoparticles as a practical alternative to toxic metal ion based QDs for biological applications.


2008 ◽  
Vol 8 (3) ◽  
pp. 1019-1051 ◽  
Author(s):  
Wei Chen

Fluorescence is widely used in biological detection and imaging. The emerging luminescent nanoparticles or quantum dots provide a new type of biological agents that can improve these applications. The advantages of luminescent nanoparticles for biological applications include their high quantum yield, color availability, good photo-stability, large surface-to-volume ratio, surface functionality, and small size. In this review article, we first introduce quantum size confinement, photoluminescence and upconversion luminescence of nanoparticles, then describe the preparation and conjugation of water soluble nanoparticles and introduce the applications of luminescence nanoparticles for in vitro and in vivo imaging, fluorescence resonance energy based detection, and the applications of luminescence nanoparticles for photodynamic activation.


2013 ◽  
Vol 4 ◽  
pp. 895-902 ◽  
Author(s):  
Irina V Martynenko ◽  
Anna O Orlova ◽  
Vladimir G Maslov ◽  
Alexander V Baranov ◽  
Anatoly V Fedorov ◽  
...  

The photoexcitation energy transfer is found and investigated in complexes of CdSe/ZnS cationic quantum dots and chlorin e6 molecules formed by covalent bonding and electrostatic interaction in aqueous solution and in porous track membranes. The quantum dots and chlorin e6 molecules form stable complexes that exhibit Förster resonance energy transfer (FRET) from quantum dots to chlorin e6 regardless of complex formation conditions. Competitive channels of photoexcitation energy dissipation in the complexes, which hamper the FRET process, were found and discussed.


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