scholarly journals Plant Growth Promotion Potential Is Equally Represented in Diverse Grapevine Root-Associated Bacterial Communities from Different Biopedoclimatic Environments

2013 ◽  
Vol 2013 ◽  
pp. 1-17 ◽  
Author(s):  
Ramona Marasco ◽  
Eleonora Rolli ◽  
Marco Fusi ◽  
Ameur Cherif ◽  
Ayman Abou-Hadid ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Plant Growth ◽  
Bacterial Communities ◽  
Growth Promotion ◽  
Rrna Gene ◽  
Similar Distribution ◽  
Bacterial Assemblage ◽  
Pgp Traits ◽  
Associated Bacteria

Plant-associated bacteria provide important services to host plants. Environmental factors such as cultivar type and pedoclimatic conditions contribute to shape their diversity. However, whether these environmental factors may influence the plant growth promoting (PGP) potential of the root-associated bacteria is not widely understood. To address this issue, the diversity and PGP potential of the bacterial assemblage associated with the grapevine root system of different cultivars in three Mediterranean environments along a macrotransect identifying an aridity gradient were assessed by culture-dependent and independent approaches. According to 16S rRNA gene PCR-DGGE, the structure of endosphere and rhizosphere bacterial communities was highly diverse (P=0.03) and was associated with a cultivar/latitudinal/climatic effect. Despite being diverse, the bacterial communities associated with Egyptian grapevines shared a higher similarity with the Tunisian grapevines than those cultivated in North Italy. A similar distribution, according to the cultivar/latitude/aridity gradients, was observed for the cultivable bacteria. Many isolates (23%) presentedin vitromultiple stress resistance capabilities and PGP activities, the most frequent being auxin synthesis (82%), insoluble phosphate solubilisation (61%), and ammonia production (70%). The comparable numbers and types of potential PGP traits among the three different environmental settings indicate a strong functional homeostasis of beneficial bacteria associated with grape root.

scholarly journals Multiple Plant Growth-Promotion Traits in Endophytic Bacteria Retrieved in the Vegetative Stage From Passionflower

2021 ◽  
Vol 11 ◽  
Author(s):  
Luis Gabriel Cueva-Yesquén ◽  
Marcela Cristina Goulart ◽  
Derlene Attili de Angelis ◽  
Marcos Nopper Alves ◽  
Fabiana Fantinatti-Garboggini
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Vegetative Stage ◽  
Rrna Gene ◽  
Culturable Bacteria ◽  
Bacterial Endophytes ◽  
Pgp Traits

Bacteria exhibiting beneficial traits like increasing the bioavailability of essential nutrients and modulating hormone levels in plants are known as plant growth promoting (PGP) bacteria. The occurrence of this specific group of bacteria in the endophytic environment may reflect the decisive role they play in a particular condition. This study aimed to determine the taxonomical diversity of the culturable bacterial endophytes, isolated in the vegetative stage of passionflower (Passiflora incarnata), and assess its potential to promote plant growth by phenotypic and genotypic approaches. The sequencing and phylogenetic analysis of the 16S rRNA gene allowed us to classify 58 bacterial endophytes into nine genera. Bacillus (70.7%) was the most dominant genus, followed by Pseudomonas (8.6%) and Pantoea (6.9%). A few isolates belonged to Rhodococcus and Paenibacillus, whereas the genera Lysinibacillus, Microvirga, Xanthomonas, and Leclercia were represented by only one isolate. The strains were tested for nitrogen fixation, phosphate solubilization, indole-acetic-acid synthesis, and siderophore production. Moreover, PGP related genes (nifH, ipdC, asb, and AcPho) were detected by PCR-based screening. Most of the isolates (94.8%) displayed a potential for at least one of the PGP traits tested by biochemical assays or PCR-based screening. Nine strains were selected based on results from both approaches and were evaluated for boosting the Cape gooseberry (Physalis peruviana) germination and growth. All tested isolates improved germination in vitro, and the majority (78%) increased growth parameters in vivo. The results suggested that most of culturable bacteria inhabiting P. incarnata in the vegetative stage could be used as probiotics for agricultural systems. Besides, their occurrence may be associated with specific physiological needs typical of this development stage.

scholarly journals Isolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L.

2016 ◽  
Vol 68 (2) ◽  
pp. 363-374 ◽  
Author(s):  
Pankaj Kumar ◽  
Ramesh Dubey ◽  
Dinesh Maheshwari ◽  
Yong-Ha Park ◽  
Vivek Bajpai
Keyword(s):  
Phaseolus Vulgaris ◽  
Plant Growth ◽  
Growth Promotion ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Pseudomonas Sp ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

In vitro screening of plant growth-promoting (PGP) traits was carried out using eight Pseudomonas spp., PPR1 to PPR8, isolated from the rhizosphere of Phaseolus vulgaris growing on the Uttarakhand Himalayan range in India. All the isolates were fast growers, positive for catalase, oxidase and urease activities, and utilized lactose and some amino acids. All the isolates were indole acetic acid (IAA) positive, however PPR8 solubilized potassium and zinc along with various other types of inorganic (tricalcium, dicalcium and zinc phosphate) and organic (calcium phytate) phosphates, as well as producing siderophore and ACC deaminase. PPR8 also produced cyanogens, extracellular chitinase, ?-1,3-glucanase, ?-1,4-glucanase and oxalate oxidase. Based on the PGP traits of all isolates, PPR8 was found to be the most potent plant growth-promoting rhizobacteria (PGPR). Further, PPR8 was identified as Pseudomonas sp. PPR8, based on 16S rRNA gene sequencing analysis. Moreover, the PGP activities of PPR8 confirmed it to be a potent biocontrol agent, inhibiting the growth of various plant pathogenic fungi. This study reveals the potential of Pseudomonas sp. PPR8 to be used as a good bioinoculant for growth promotion of common bean and for the protection of important legume crops from various deleterious phytopathogens.

scholarly journals Exploitation of endophytic Pseudomonas sp. for plant growth promotion and colonization in rice

2017 ◽  
Vol 9 (3) ◽  
pp. 1310-1316
Author(s):  
Gurjot Kaur ◽  
Poonam Sharma ◽  
Deepika Chhabra ◽  
Kailash Chand ◽  
Gurjit Singh Mangat
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Pseudomonas Sp ◽  
Bacterial Isolates ◽  
Pgp Traits ◽  
P Solubilization ◽  
Fluorescent Pigment

The present investigation was carried out to exploit bacterial endophytes associated with root and leaf tissue of rice plant for plant growth promotion (PGP) and colonization study in vitro. Total 10 endophytic bacterial isolates (Pseudomonas sp.) were evaluate for PGP traits like P solubilization, production of Indole acetic acid (IAA), siderophore, ACC deaminase, protease, cellulase, fluorescent pigment, urease and denitrification activity. Out of 10 endophytic bacteria 30 %, 60 %, 20 %, 70 %, 10 % and 10 % were positive for siderophore, protease, cellulase, fluorescent pigment, urease and denitrification respectively. Maximum IAA production was recorded with isolate LRBLE7 (18.8 μgml-1) followed by LRBRE4 (16.0 μgml-1) and maximum P-solubilization was recorded with isolate LRBRE4 (5.8 mg 100 ml-1) followed by LRBLE7 (4.4 mg 100 ml-1). ACC deaminase production was recorded with isolate LRBLE6 (O.D=0.352 nm) followed by LRBRE5 (O.D=0.324nm). Three potential isolates (LRBRE4, LRBRE6 and LRBLE7) were selected on the basis of multiple PGP traits and were subjected to colonization study of rice seedling in vitro. Potential bacterial isolates can be exploited for improving growth and productivity in rice under sustainable management system.

scholarly journals Appraising Endophyte–Plant Symbiosis for Improved Growth, Nodulation, Nitrogen Fixation and Abiotic Stress Tolerance: An Experimental Investigation with Chickpea (Cicer arietinum L.)

Agronomy ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 621 ◽  
Author(s):  
Ahmad ◽  
Naseer ◽  
Hussain ◽  
Zahid Mumtaz ◽  
Mustafa ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Root Nodules ◽  
Abiotic Stress Tolerance ◽  
Paenibacillus Polymyxa ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Pgp Traits ◽  
Paenibacillus Sp

Chickpea is an important leguminous crop that improves soil fertility through atmospheric nitrogen fixation with the help of rhizobia present in nodules. Non-rhizobia endophytes are also capable of inducing nodulation and nitrogen fixation in leguminous crops. The aim of the current study was to isolate, characterize and identify the non-rhizobia endophytic bacterial strains from root nodules of chickpea. For this purpose, more than one hundred isolates were isolated from chickpea root nodules under aseptic conditions and were confirmed as endophytes through re-isolating them from root nodules of chickpea after their inoculation. Nineteen confirmed endophytic bacterial strains revealed significant production of indole acetic acid (IAA) both in presence and absence of L-tryptophan and showed their ability to grow under salt, pH and heavy metal stresses. These strains were evaluated for in vitro plant growth promoting (PGP) traits and results revealed that seven strains showed solubilization of P and colloidal chitin along with possessing catalase, oxidase, urease and chitinase activities. Seven P-solubilizing strains were further evaluated in a jar trial to explore their potential for promoting plant growth and induction of nodulation in chickpea roots. Two endophytic strains identified as Paenibacillus polymyxa ANM59 and Paenibacillus sp. ANM76 through partial sequencing of the 16S rRNA gene showed the maximum potential during in vitro PGP activities and improved plant growth and nodulation in chickpea under the jar trial. Use of these endophytic strains as a potential biofertilizer can help to reduce the dependence on chemical fertilizers while improving crop growth and soil health simultaneously.

scholarly journals Diversity, Phylogeny and Plant Growth Promotion Traits of Nodule Associated Bacteria Isolated from Lotus parviflorus

2020 ◽  
Vol 8 (4) ◽  
pp. 499 ◽  
Author(s):  
Ricardo Soares ◽  
Jesús Trejo ◽  
Maria J. Lorite ◽  
Etelvina Figueira ◽  
Juan Sanjuán ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Root Nodules ◽  
Nifh Gene ◽  
Bacterial Strains ◽  
Lytic Enzymes ◽  
Taxonomic Assignment ◽  
Pgp Traits ◽  
Associated Bacteria

Lotus spp. are widely used as a forage to improve pastures, and inoculation with elite rhizobial strains is a common practice in many countries. However, only a few Lotus species have been studied in the context of plant-rhizobia interactions. In this study, forty highly diverse bacterial strains were isolated from root nodules of wild Lotus parviflorus plants growing in two field locations in Portugal. However, only 10% of these isolates could nodulate one or more legume hosts tested, whereas 90% were thought to be opportunistic nodule associated bacteria. Phylogenetic studies place the nodulating isolates within the Bradyrhizobium genus, which is closely related to B. canariense and other Bradyrhizobium sp. strains isolated from genistoid legumes and Ornithopus spp. Symbiotic nodC and nifH gene phylogenies were fully consistent with the taxonomic assignment and host range. The non-nodulating bacteria isolated were alpha- (Rhizobium/Agrobacterium), beta- (Massilia) and gamma-proteobacteria (Pseudomonas, Lysobacter, Luteibacter, Stenotrophomonas and Rahnella), as well as some bacteroidetes from genera Sphingobacterium and Mucilaginibacter. Some of these nodule-associated bacteria expressed plant growth promotion (PGP) traits, such as production of lytic enzymes, antagonistic activity against phytopathogens, phosphate solubilization, or siderophore production. This argues for a potential beneficial role of these L. parviflorus nodule-associated bacteria.

scholarly journals Plant Growth-Promoting Activity of Pseudomonas aeruginosa FG106 and Its Ability to Act as a Biocontrol Agent against Potato, Tomato and Taro Pathogens

Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 140
Author(s):  
Farideh Ghadamgahi ◽  
Saeed Tarighi ◽  
Parissa Taheri ◽  
Ganapathi Varma Saripella ◽  
Alice Anzalone ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Genome Mining ◽  
16S Rrna Gene Sequencing ◽  
Control Agent ◽  
Rrna Gene ◽  
Clavibacter Michiganensis Subsp ◽  
Ability To Act

P. aeruginosa strain FG106 was isolated from the rhizosphere of tomato plants and identified through morphological analysis, 16S rRNA gene sequencing, and whole-genome sequencing. In vitro and in vivo experiments demonstrated that this strain could control several pathogens on tomato, potato, taro, and strawberry. Volatile and non-volatile metabolites produced by the strain are known to adversely affect the tested pathogens. FG106 showed clear antagonism against Alternaria alternata, Botrytis cinerea, Clavibacter michiganensis subsp. michiganensis, Phytophthora colocasiae, P. infestans, Rhizoctonia solani, and Xanthomonas euvesicatoria pv. perforans. FG106 produced proteases and lipases while also inducing high phosphate solubilization, producing siderophores, ammonia, indole acetic acid (IAA), and hydrogen cyanide (HCN) and forming biofilms that promote plant growth and facilitate biocontrol. Genome mining approaches showed that this strain harbors genes related to biocontrol and growth promotion. These results suggest that this bacterial strain provides good protection against pathogens of several agriculturally important plants via direct and indirect modes of action and could thus be a valuable bio-control agent.

scholarly journals Bacteria Isolated from Wastewater Irrigated Agricultural Soils Adapt to Heavy Metal Toxicity While Maintaining Their Plant Growth Promoting Traits

2021 ◽  
Vol 13 (14) ◽  
pp. 7792
Author(s):  
Abdul Wahab Ajmal ◽  
Saleha Saroosh ◽  
Shah Mulk ◽  
Muhammad Nadeem Hassan ◽  
Humaira Yasmin ◽  
...  
Keyword(s):  
Plant Growth ◽  
Agricultural Soils ◽  
Culture Media ◽  
Growth Promotion ◽  
Metal Resistance ◽  
Rrna Gene ◽  
Gene Sequence Analysis ◽  
Plant Growth Promoting ◽  
Growth Promoting

The present study explored the plant growth promotion and bioremediation potential of bacteria inhabiting wastewater irrigated agricultural soils. Thirty out of 75 bacterial isolates (40%), 29/75 (39%) and 28/75 (37%) solubilized Zn, K and PO4 during plate essays respectively. Fifty-six percent of the isolates produced siderophores, while 30% released protease in vitro. Seventy-four percent of bacteria resisted Pb, Ni and Cd at various concentrations added to the culture media plates. Sixteen out of 75 (26%) isolates were able to fix N in Nbf medium. Among these 16 N fixers, N fixing nifH, nifD and nifK genes was detected through PCR in 8, 7 and 1 strain respectively using gene specific primers designed in the study with Enterobacter sp. having all three (nifHKD) genes. Isolated bacteria showed resemblance to diverse genera such as Bacillus, Pseudomonas, Enterobacter, Citrobacter, Acinetobacter, Serratia, Klebsiella and Enterococcus based on 16S rRNA gene sequence analysis. In addition to showing the best mineral solubilization and metal resistance potential, Citrobacter sp. and Enterobacter sp. also removed 87%, 79% and 43% and 86%, 78% and 51% of Ni, Cd and Pb, respectively, from aqueous solution. These potent bacteria may be exploited both for bioremediation and biofertilization of wastewater irrigated soils leading to sustainable agriculture.

scholarly journals Screening of efficient rhizobacteria associated with cauliflower (Brassica oleraceavar. botrytis L.) for plant growth promoting traits

2017 ◽  
Vol 9 (1) ◽  
pp. 167-172
Author(s):  
Sonal Bhardwaj ◽  
Bhawna Dipta ◽  
Shruti Kirti ◽  
Rajesh Kaushal
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Himachal Pradesh ◽  
Pgp Traits ◽  
Antifungal Metabolites ◽  
Stalk Rot ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

In the current study, a total of 25 isolates were isolated from the rhizosphere and roots of cauliflower (Brassica oleraceavar. botrytis L.) from the vicinity of Una district of Himachal Pradesh. The isolates were tested in vitro for their ability to solubilise phosphorous and produce siderophore, indole acetic acid (IAA), hydrogen cyanide (HCN) and antifungal metabolites against the soil borne pathogens. Results revealed that out of 25, only 4 rhizospheric isolates (SB5, SB11, SB8 and SB10) have maximum plant growth promoting attributes. The isolates were identified as Bacillus sp. on the basis of Bergey’s manual of systematic bacteriology. The isolate SB11 recorded highest phosphate solubilizing efficiency in solid medium (109.09%) and in liquid medium (350μg/ml). Maximum production of IAA (51.96μg/ml), siderophore (91.41%) and HCN were also observed for the same isolate. Further-more, the isolate SB11 produced highest antifungal metabolite production against Rhizoctoniasolani(37.11%), Sclerotiniasclerotiorum(41.11%), and Pythium sp. (71.11%) causing root rot, stalk rot and damping off diseases in cauliflower, respectively. The selected isolate (SB11) showed optimum growth at a pH of 7.0, 35°C temperature and 2% NaCl. On the basis of multifarious PGP-traits the SB11 isolate has tremendous potential to be used as a bioferti-lizer/bioprotectant for growth promotion and natural protection of cauliflower under low hill conditions of Himachal Pradesh.

scholarly journals Genomic Analysis of the Endophytic Stenotrophomonas Strain 169 Reveals Features Related to Plant-Growth Promotion and Stress Tolerance

2021 ◽  
Vol 12 ◽  
Author(s):  
Kristina Ulrich ◽  
Michael Kube ◽  
Regina Becker ◽  
Volker Schneck ◽  
Andreas Ulrich
Keyword(s):  
Plant Growth ◽  
Stress Tolerance ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Whole Genome Sequence ◽  
Plant Colonization ◽  
Rrna Gene ◽  
Promote Plant Growth ◽  
Physiological Tests

Plant-associated Stenotrophomonas isolates have great potential for plant growth promotion, especially under stress conditions, due to their ability to promote tolerance to abiotic stresses such as salinity or drought. The endophytic strain Stenotrophomonas sp. 169, isolated from a field-grown poplar, increased the growth of inoculated in vitro plants, with a particular effect on root development, and was able to stimulate the rooting of poplar cuttings in the greenhouse. The strain produced high amounts of the plant growth-stimulating hormone auxin under in vitro conditions. The comparison of the 16S rRNA gene sequences and the phylogenetic analysis of the core genomes showed a close relationship to Stenotrophomonas chelatiphaga and a clear separation from Stenotrophomonas maltophilia. Whole genome sequence analysis revealed functional genes potentially associated with attachment and plant colonization, growth promotion, and stress protection. In detail, an extensive set of genes for twitching motility, chemotaxis, flagella biosynthesis, and the ability to form biofilms, which are connected with host plant colonization, could be identified in the genome of strain 169. The production of indole-3-acetic acid and the presence of genes for auxin biosynthesis pathways and the spermidine pathway could explain the ability to promote plant growth. Furthermore, the genome contained genes encoding for features related to the production of different osmoprotective molecules and enzymes mediating the regulation of stress tolerance and the ability of bacteria to quickly adapt to changing environments. Overall, the results of physiological tests and genome analysis demonstrated the capability of endophytic strain 169 to promote plant growth. In contrast to related species, strain 169 can be considered non-pathogenic and suitable for biotechnology applications.

scholarly journals Unveiling the Microbiota Diversity of the Xerophyte Argania spinosa L. Skeels Root System and Residuesphere

2020 ◽  
Vol 80 (4) ◽  
pp. 822-836 ◽  
Author(s):  
Francesca Mapelli ◽  
Valentina Riva ◽  
Lorenzo Vergani ◽  
Redouane Choukrallah ◽  
Sara Borin
Keyword(s):  
Root System ◽  
Bacterial Communities ◽  
Growth Promotion ◽  
Economic Value ◽  
16S Rrna Gene Sequencing ◽  
Water Shortage ◽  
Rrna Gene ◽  
Alpha And Beta Diversity ◽  
Argania Spinosa

Abstract The microbiota associated to xerophyte is a “black box” that might include microbes involved in plant adaptation to the extreme conditions that characterize their habitat, like water shortage. In this work, we studied the bacterial communities inhabiting the root system of Argania spinosa L. Skeels, a tree of high economic value and ecological relevance in Northern Africa. Illumina 16S rRNA gene sequencing and cultivation techniques were applied to unravel the bacterial microbiota’s structure in environmental niches associated to argan plants (i.e., root endosphere, rhizosphere, root-surrounding soil), not associated to the plant (i.e., bulk soil), and indirectly influenced by the plant being partially composed by its leafy residue and the associated microbes (i.e., residuesphere). Illumina dataset indicated that the root system portions of A. spinosa hosted different bacterial communities according to their degree of association with the plant, enriching for taxa typical of the plant microbiome. Similar alpha- and beta-diversity trends were observed for the total microbiota and its cultivable fraction, which included 371 isolates. In particular, the residuesphere was the niche with the highest bacterial diversity. The Plant Growth Promotion (PGP) potential of 219 isolates was investigated in vitro, assessing several traits related to biofertilization and biocontrol, besides the production of exopolysaccharides. Most of the multivalent isolates showing the higher PGP score were identified in the residuesphere, suggesting it as a habitat that favor their proliferation. We hypothesized that these bacteria can contribute, in partnership with the argan root system, to the litter effect played by this tree in its native arid lands.

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