The Efficacy of Molecular Markers Analysis with Integration of Sensory Methods in Detection of Aroma in Rice

Allele Specific Amplification with four primers (External Antisense Primer, External Sense Primer, Internal Nonfragrant Sense Primer, and Internal Fragrant Antisense Primer) and sensory evaluation with leaves and grains were executed to identify aromatic rice genotypes and their F1individuals derived from different crosses of 2 Malaysian varieties with 4 popular land races and 3 advance lines. Homozygous aromatic (fgr/fgr) F1individuals demonstrated better aroma scores compared to both heterozygous nonaromatic (FGR/fgr) and homozygous nonaromatic (FGR/FGR) individuals, while, some F1individuals expressed aroma in both leaf and grain aromatic tests without possessing thefgrallele. Genotypic analysis of F1individuals for thefgrgene represented homozygous aromatic, heterozygous nonaromatic and homozygous nonaromatic genotypes in the ratio 20 : 19 : 3. Genotypic and phenotypic analysis revealed that aroma in F1individuals was successfully inherited from the parents, but either molecular analysis or sensory evaluation alone could not determine aromatic condition completely. The integration of molecular analysis with sensory methods was observed as rapid and reliable for the screening of aromatic genotypes because molecular analysis could distinguish aromatic homozygous, nonaromatic homozygous and nonaromatic heterozygous individuals, whilst the sensory method facilitated the evaluation of aroma emitted from leaf and grain during flowering to maturity stages.

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Physico-chemical and Genetic Analysis of Aromatic Rice (Oryza sativa L.) Germplasm

The Agriculturists ◽

10.3329/agric.v9i1-2.9482 ◽

1970 ◽

Vol 9 (1-2) ◽

pp. 82-88 ◽

Cited By ~ 5

Author(s):

ZA Jewel ◽

AK Patwary ◽

S Maniruzzaman ◽

R Barua ◽

SN Begum

Keyword(s):

Grain Quality ◽

Rice Variety ◽

Negative Association ◽

Width Ratio ◽

Aromatic Rice ◽

Yield Attributes ◽

Sensory Test ◽

Grain Length ◽

Genotypic Analysis ◽

Rice Genotypes

For selection of aromatic rice lines, twenty six (26) rice genotypes were evaluated for agronomic characteristics and aroma detection through sensory test and genotypic analysis using SSR markers. Grain quality and yield attributes were analyzed after harvesting the grain. Aroma was detected by 1.7% KOH as a sensory test. Based on sensory test, six genotypes were detected for having strong aroma; 7 for moderate aroma; 10 for slight aroma and 3 for no aroma. Aroma had significant and positive association with grain length-width ratio; significant and negative association with grain width, significant and negative association with gelatinization temperature, and no significant association with grain length. Three SSR primers viz; RM223, RM515 and RM342 were used for identifying fgr gene locus in 26 rice genotypes. The primer RM223 identified the fgr locus as homozygous condition for 6 as strong aromatic, 7 moderate aromatic, 10 slight aromatic and the rest 3 as non aromatic. The primer RM515 detected 4 as strong aromatic, 6 as moderate aromatic, and 16 as slight to non aromatic. The primer RM342 detected 3 as strong aromatic and four as moderate aromatic, 19 as slight to non aromatic. Compared among the three markers, RM223 detected the highest number of fgr locus in aromatic rice genotypes. Among the three primers, RM223 responded best in all the 26 rice genotypes, because RM223 primer could be able to identify aromatic and nonaromatic genes having higher yield with good agronomic performance and other grain quality traits. These elite lines could be readily used in breeding programme for release aromatic rice variety with considerable yield. Keywords: Microsatellite markers; aromatic rice DOI: http://dx.doi.org/10.3329/agric.v9i1-2.9482 The Agriculturists 2011; 9(1&2): 82-88

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Rh E/e genotyping by allele-specific primer amplification

Blood ◽

10.1182/blood.v85.3.829.bloodjournal853829 ◽

1995 ◽

Vol 85 (3) ◽

pp. 829-832 ◽

Cited By ~ 29

Author(s):

BH Faas ◽

S Simsek ◽

PM Bleeker ◽

MA Overbeeke ◽

HT Cuijpers ◽

...

Keyword(s):

Nucleotide Position ◽

Blood Group Antigens ◽

Specific Primer ◽

Nucleotide Difference ◽

Homologous Genes ◽

Allele Specific ◽

Antisense Primer ◽

Polymerase Chain ◽

Sense Primer ◽

Rh Blood Group

It has been shown that the Rhesus (Rh) blood group antigens are encoded by two homologous genes: the Rh D gene and the Rh CcEe gene. The Rh CcEe gene encodes different peptides: the Rh C, c, E, and e polypeptides. Only one nucleotide difference has been found between the alleles encoding the Rh E and the Rh e antigen polypeptides. It is a C-- >G transition at nucleotide position 676, which leads to an amino acid substitution from proline to alanine in the Rh e-carrying polypeptide. Here we present an allele-specific primer amplification (ASPA) method to determine the Rh E and Rh e genotypes. In one polymerase chain reaction, the sense primer had a 3′-end nucleotide specific for the cytosine at position 676 of the Rh E allele. In another reaction, a sense primer was used with a 3′-end nucleotide specific for the guanine at position 676 of the Rh e allele and the Rh D gene, whereas the antisense primer had a 3′-end nucleotide specific for the adenine at position 787 of the Rh CcEe gene. We tested DNA samples from 158 normal donors (including non-Caucasian donors and donors with rare Rh phenotypes) in these assays. There was full concordance with the results of serologic Rh E/e phenotyping. Thus, we may conclude that the ASPA approach leads to a simple and reliable method to determine the Rh E/e genotype. This can be useful in Rh E/e genotyping of fetuses and/or in cases in which no red blood cells are available for serotyping. Moreover, our results confirm the proposed association between the cytosine/guanine polymorphism at position 676 and the Rh E/e phenotype.

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Screening of aromatic rice lines by phenotypic and molecular markers

Bangladesh Journal of Botany ◽

10.3329/bjb.v37i2.1720 ◽

1970 ◽

Vol 37 (2) ◽

pp. 141-147 ◽

Cited By ~ 10

Author(s):

K Kibria ◽

MM Islam ◽

SN Begum

Keyword(s):

Ssr Markers ◽

Grain Quality ◽

Negative Association ◽

Yield Potential ◽

Rice Grain ◽

Agronomic Performance ◽

Aromatic Rice ◽

Genotypic Analysis ◽

Rice Genotypes ◽

Segregating Population

To improve the yield potential of local aromatic variety Kalizira, a segregating population was developed from a cross between Y-1281 (high yielding mutant variety) and Kalizira. Thirty two F7 rice lines were used to evaluate agronomic characteristics, aroma detection through sensory test and genotypic analysis using microsatellite markers. Highly significant negative association was found between aroma and grain yield. Nine, 12 and 17 pedigree lines (PLs) having fragrance gene (fgr) locus were found using three SSR markers RM223, RM342A and RM515, respectively as homozygous condition in 32 rice lines. The marker RM515 detected highest number of fgr locus in PLs. Fourteen promising lines were identified with aroma genes having higher yield with good agronomic performance and other grain quality traits. These SSR markers could be utilized in marker-assisted selection (MAS) and would have a great impact on identifying fgr locus in rice genotypes. Key words: Aromatic rice, Grain quality, Microsatellite marker doi:10.3329/bjb.v37i2.1720 Bangladesh J. Bot. 37(2): 141-147, 2008 (December)

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Simple and Rapid Detection of Factor V Leiden by Allele-specific PCR Amplification

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650362 ◽

1996 ◽

Vol 75 (05) ◽

pp. 757-759 ◽

Cited By ~ 11

Author(s):

Rainer Blasczyk ◽

Markus Ritter ◽

Christian Thiede ◽

Jenny Wehling ◽

Günter Hintz ◽

...

Keyword(s):

Direct Sequencing ◽

Activated Protein C ◽

Factor V Leiden ◽

Pcr Amplification ◽

Factor V ◽

Specific Pcr ◽

Pcr Rflp ◽

Allele Specific ◽

Specific Amplification ◽

Allele Specific Pcr

SummaryResistance to activated protein C is the most common hereditary cause for thrombosis and significantly linked to factor V Leiden. In this study, primers were designed to identify the factor V mutation by allele-specific PCR amplification. 126 patients with thromboembolic events were analysed using this technique, PCR-RFLP and direct sequencing. The concordance between these techniques was 100%. In 27 patients a heterozygous factor VGln506 mutation was detected, whereas one patient with recurrent thromboembolism was homozygous for the point mutation. Due to its time- and cost-saving features allele-specific amplification should be considered for screening of factor VGln506.

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