scholarly journals Characterization of Thermo- and Detergent Stable Antigenic Glycosylated Cysteine Protease ofEuphorbia nivuliaBuch.-Ham. and Evaluation of Its Ecofriendly Applications

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Shamkant B. Badgujar ◽  
Raghunath T. Mahajan

An antigenic glycosylated cysteine protease has been purified from the latex ofEuphorbia nivuliaBuch.-Ham. It exhibits remarkable protease activity in the presence of metal ions, oxidizing agents, organic solvents, and detergents. This enzyme showed potential role in leather processing industry due to its dehairing activity for animal hide without hydrolyzing fibrous proteins, producing, by this way, a better quality product. The enzyme can also be used for silver recovering from X-ray plates. In addition, the stability (temperature and surfactants) and hydrolysis of blood stain data also revealed its application in detergent industries. Agriculturally, this protease finds application in biocontrol process against the infectious management of root knot nematode,Meloidogyne incognita. Biologically, it shows noticeable wound healing, haemostatic and antibacterial activity.

Polymers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1979
Author(s):  
Ho In Lee ◽  
Yun Heo ◽  
Seung-Woon Baek ◽  
Da-Seul Kim ◽  
Duck Hyun Song ◽  
...  

Poly(L-lactic acid) (PLLA) has been used as a biodegradable vascular scaffold (BVS) material due to high mechanical property, biodegradability, and biocompatibility. However, acidic byproducts from hydrolysis of PLLA reduce the pH after the surrounding implanted area and cause inflammatory responses. As a result, severe inflammation, thrombosis, and in-stent restenosis can occur after implantation by using BVS. Additionally, polymers such as PLLA could not find on X-ray computed tomography (CT) because of low radiopacity. To this end, here, we fabricated PLLA films as the surface of BVS and divided PLLA films into two coating layers. At the first layer, PLLA film was coated by 2,3,5-triiodobenzoic acid (TIBA) and magnesium hydroxide (MH) with poly(D,L-lactic acid) (PDLLA) for radiopaque and neutralization of acidic environment, respectively. The second layer of coated PLLA films is composed of polydopamine (PDA) and then cystamine (Cys) for the generation of nitric oxide (NO) release, which is needed for suppression of smooth muscle cells (SMCs) and proliferation of endothelial cells (ECs). The characterization of the film surface was conducted via various analyses. Through the surface modification of PLLA films, they have multifunctional abilities to overcome problems of BVS effectively such as X-ray penetrability, inflammation, thrombosis, and neointimal hyperplasia. These results suggest that the modification of biodegradable PLLA using TIBA, MH, PDA, and Cys will have important potential in implant applications.


2020 ◽  
Vol 100 (1) ◽  
pp. 28
Author(s):  
Anupam ◽  
Narpinderjeet Kaur Dhillon ◽  
Sukhjeet Kaur ◽  
Salesh Kumar Jindal ◽  
Harwinder Singh Buttar

BMC Genomics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Clémence Medina ◽  
Martine da Rocha ◽  
Marc Magliano ◽  
Alizée Raptopoulo ◽  
Nathalie Marteu ◽  
...  

2014 ◽  
Vol 30 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Yunhee Seo ◽  
Jiyeong Park ◽  
Yong Su Kim ◽  
Yong Park ◽  
Young Ho Kim

2020 ◽  
Vol 14 (4) ◽  
pp. 453-460
Author(s):  
Ruyi Sha ◽  
Zhan Yu ◽  
Zhenzhen Wang ◽  
Edwin Menledy Gbor ◽  
Ligang Jiang ◽  
...  

The lignin present in lignocellulose seriously affects the efficiency of cellulose enzymatic hydrolysis. In addition, lignin adsorbs high-cost cellulase, causing greater economic losses. Lignin can also disturb the site of action of cellulase and reduce the efficiency of hydrolysis. Therefore, if lignin is removed or surface modified before cellulose enzymatic hydrolysis, the enzymatic hydrolysis efficiency of lignocellulosic biomass will be greatly improved. In this paper, the cellulose enzymatic properties of bamboo biomass being treated with dilute acid and alkaline under the intervention of biosurfactant rhamnolipid were evaluated. The effects of rhamnolipids on the adsorption characterization of cellulose on pretreated bamboo were studied. Besides, the inter-communication between rhamnolipids and cellulose was investigated by fluorescence probe. The results showed that rhamnolipids could have a positive effect on the enzymatic hydrolysis of bamboo biomass by reducing the non-productive adsorption of cellulase on the surface of lignocellulose. The outcome illustrated that cellulase could be combined with rhamnolipids micelles, participating in the formation of rhamnolipids micelles, thereby increasing the internal hydrophobicity of the micelles, but could not change the properties of rhamnolipids micelles higher than one CMC (Critical Micelle Concentration). It can be seen that the interaction between rhamnolipids and cellulase is beneficial to enhance the stability and enzymatic activity of cellulase, thereby improving the enzymatic hydrolysis efficiency of cellulose in biomass. Based on these results, a theoretical knowledge about the mechanism of enhancing the enzymatic hydrolysis efficiency of lignocellulose by biosurfactants rhamnolipids is provided.


1995 ◽  
Vol 385 ◽  
Author(s):  
Barry J. Bauer ◽  
Catheryn L. Jackson ◽  
Da-Wei Liu

ABSTRACTInterpenetrating polymer networks have been synthesized by performing sol-gel chemistry and conventional organic polymerizations in mixtures of the monomers. The organic polymers were acrylates, and the inorganic phase was SiO2 formed by hydrolysis of orthosilicates. Polymerizations were conducted at a variety of relative rates, and the chemistry was designed to allow different amounts of grafting between the components. The morphology was characterized by transmission electron microscopy and small angle neutron and x-ray scattering. Wide variations in morphology were observed depending on the polymerization conditions, ranging from grossly phase separated to dendritic to finely divided structures (at a 100Å size scale). The phases ranged from mixtures of the two components to relatively pure phases. The interface between the phases ranged from very narrow to relatively broad.


Science ◽  
2017 ◽  
Vol 355 (6323) ◽  
pp. 374-376 ◽  
Author(s):  
Chong Zhang ◽  
Chengguo Sun ◽  
Bingcheng Hu ◽  
Chuanming Yu ◽  
Ming Lu

Pentazole (HN5), an unstable molecular ring comprising five nitrogen atoms, has been of great interest to researchers for the better part of a century. We report the synthesis and characterization of the pentazolate anion stabilized in a (N5)6(H3O)3(NH4)4Cl salt. The anion was generated by direct cleavage of the C–N bond in a multisubstituted arylpentazole using m-chloroperbenzoic acid and ferrous bisglycinate. The structure was confirmed by single-crystal x-ray diffraction analysis, which highlighted stabilization of the cyclo-N5ˉ ring by chloride, ammonium, and hydronium. Thermal analysis indicated the stability of the salt below 117°C on the basis of thermogravimetry-measured onset decomposition temperature.


The Ruddlesden-Popper structural family is constructed from ordered intergrowths of rocksalt type (AX) layers with perovskite (ABX 3 ) blocks of varying widths, yielding phases with the general formula n ABX 3 AX where 1 < n < ∞ In this article, the characterization of (Ca x Sr 1-x ) n+1 Ti n O 3n+1 layer perovskites by powder X-ray and neutron diffraction and high resolution electron microscopy is described. These phases undergo a phase transition from tetragonal to orthorhombic symmetry at x = 0.65. Structure solutions of neutron diffraction data are presented for the end-member phases Sr 3 Ti 2 O 7 (I4/mmm), Ca 3 Ti 2 O 7 (Ccm2 1 ) and Ca 4 Ti 3 O 10 (Pcab). Refinement of the mixed alkaline earth preparation (Ca 0.85 Sr 0.15 ) 4 Ti 3 O 10 showed that Sr partitioned preferentially to the perovskite blocks rather than the rocksalt layers. Ordered and disordered intergrowths of rocksalt layers were found on the{101} orth = {100} tet perovskite planes with extensive disorder most prevalent in samples annealed for short periods (less than 24 hours). Evidence is presented for the existence of V"A and V"," point defects in the intermediate structures that precede the formation of ordered layer sequences. These data are discussed in terms of existing thermodynamic and structural information. For generalized Ruddlesden Popper phases the relation between chemistry and symmetry is reviewed and factors contributing to the stability of different n -members enunciated. For completeness, the (Ca x Sr 1-x ) n+1 Ti n O 3n+1 perovskites are considered as the structural prototypes of the oxide superconductors being a subset of an extended n ABX 3 mAX Ruddlesden-Popper family.


2008 ◽  
Vol 2008 ◽  
pp. 1-6 ◽  
Author(s):  
Guido Ennas ◽  
Maria F. Casula ◽  
Sergio Marras ◽  
Gabriele Navarra ◽  
Alessandra Scano ◽  
...  

A nanocomposite with an FeOOH/SiO2ratio equal to 17.7 wt% and the pertinent matrix, obtained by etching away the nanoparticles through reaction with hydrochloric acid, were investigated by XRD, TGA-DTA, heliostereopicnometry, BET, and TEM techniques. The study shows the presence in the nanocomposite of ferrihydrite nanoparticles phase with average dimensions around 4 nm. The FeOOH nanoparticles structure was analyzed by synchrotron X-ray diffraction data using the distribution difference curve method. The porous structure of the matrix resulting by etching away the nanoparticles differs significantly from that of a pureSiO2sample obtained by hydrolysis of TEOS under the same operative conditions followed in the nanocomposite preparation.


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