scholarly journals Inhibitory Effect of a Hot-Water Extract of Leaves of Japanese Big-Leaf Magnolia (Magnolia obovata) on Rotavirus-Induced Diarrhea in Mouse Pups

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Takeshi Kawahara ◽  
Takuma Tomono ◽  
Yasunori Hamauzu ◽  
Katsumi Tanaka ◽  
Hisako Yasui
Keyword(s):  
Water Extract ◽  
Viral Proteins ◽  
Inhibitory Effect ◽  
Reversed Phase ◽  
Hot Water ◽  
Flavonoid Glycosides ◽  
Traditional Foods ◽  
Cytopathic Effects ◽  
Magnolia Obovata ◽  
Hot Water Extract

The leaf of Japanese big-leaf magnolia (Magnolia obovataThunb.) has long been used as a natural packaging material for traditional foods in Japan. However, many of the physiological functions of the leaves against oral infection and resultant illness remain unclear. The aim of the present study was to investigate the effects of a hot-water extract of the leaves ofMagnolia obovataon diarrhea induced by rotavirus (RV), a major cause of acute diarrhea. RV strain SA11 was mixed with theM. obovataleaf extract and inoculated orally to neonatal BALB/c mouse pups. Simultaneous inoculation of SA11 with the extract significantly decreased the incidence of diarrhea. In addition, the extract significantly inhibited cytopathic effects and mRNA expression of viral proteins in SA11-infected MA104 cells. Two flavonoid glycosides, quercitrin and rutin, were strongly suggested to be major anti-RV agents in the extract by serial solvent extraction and reversed-phase HPLC-ESI-MS analysis. Our results suggest that the hot-water extract ofM. obovataleaves can be used as a medicine or food additive to prevent and ameliorate RV-induced diarrhea in individuals that may have difficulty in benefitting from the RV vaccines.

scholarly journals Allelopathic Potential of Papaver Somniferum L. on Seed Germination Against Three Different Varities of Zea Mays

2021 ◽  
Author(s):  
Muntaha Tul Sidra ◽  
SAYEDA SARAH MUBARAK ◽  
FATIMA KHAN SHERWANI ◽  
NAZMA AZEEM
Keyword(s):  
Zea Mays ◽  
Aqueous Extract ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Papaver Somniferum ◽  
Hot Water ◽  
Allelopathic Effect ◽  
Inhibitory Effects ◽  
Allelopathic Potential ◽  
Hot Water Extract

Abstract Aims To evaluate the allelopathic effect of Papaver somniferum against the 3 varieties of Zea mays seeds cv. Azam, Pahari and Iqbal. Methods Aqueous extracts were made by soaking the powder of dried leaves of Papaver somniferum. For the aqueous extract treatment, the extract was soaked in distilled water for 24 and 48 hours separately. For mulching treatment, the seeds were sowed in soil and sprinkled powder of leaves. For litter, the petri dishes were grounded with randomly cut pieces of filter paper wetted with extract. For hot water treatment the water was boiled for 5 minutes. The powder was then mixed in boil water. Results Azam got effected in 24h extract in aqueous extract treatment while Pahari in 48h treatment. In mulching all the three varieties got inhibited by extract. Azam showed resistance toward the inhibitory effect of extract in litter experiments. Iqbal was affected in hot water extract. Conclusion It is concluded that the extracts obtained from Papaver somniferum showed inhibitory effects on all the three varieties except few exceptions of maize. The plumule and radical growth got affected the most.

scholarly journals Antifungal ability and decay resistance of Fokienia hodginsii heartwood extract and its inhibitory effect on Gloeophyllum trabeum

BioResources ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. 2784-2799
Author(s):  
Jian-Fei Wu ◽  
Xue-Feng Zhuang ◽  
Hong-mei Yuan ◽  
Quan Li ◽  
Wei Wang ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Mass Loss Rate ◽  
Water Extract ◽  
Decay Resistance ◽  
Inhibitory Effect ◽  
Hot Water ◽  
Gloeophyllum Trabeum ◽  
Test Pieces ◽  
Hot Water Extract ◽  
Acetone Extracts

The inhibitory ability of Fokienia hodginsii heartwood (FHH) extracts on Trametes versicolor (TV) and Gloeophyllum trabeum (GT) as well as the toxic effect of its heartwood extracts on GT were studied. The growth inhibition ability of the samples was analyzed using the growth rate method. The results showed that in the experiment of inhibiting TV, extracts using hot water had little effect, acetone extracts had the best inhibiting effect, and the lowest value of acetone EC50 was 0.409 g/L. The parameter EC50 is the concentration of the corresponding agent that inhibits the growth of 50% fungi. In the antifungal experiment of GT, methanol extract had the best inhibition effect, and the lowest EC50 value was 0.283 g/L. The antifungal effect of five solvent extracts of FHH was good when the concentration was 10% (w/w), and at this time, the mass loss rate of the test pieces was below 11%, all of them were Class I, indicating a strong antifungal level. After observing the samples of GT with SEM, it was found that the structure of methanol extract treatment was more complete and the antiseptic effect was better than that of the hot water extract treatment.

scholarly journals Synbiotic Effect of Probiotic ( Bifidobacterium sp ) and Prebiotics (Chicory and Inulin ) aganist some pathogenic bacteria

2009 ◽  
Vol 6 (2) ◽  
pp. 354-360
Author(s):  
Baghdad Science Journal
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Hot Water ◽  
Hot Water Extract ◽  
Esherichia Coli ◽  
Synergistic Inhibitory Effect ◽  
Chicory Roots

The Antimicrobial activity of Probiotic Bifidobacterium sp and Prebiotics : chicory roots ( Hot water extract ) and Inulin ( 10 % ) against some Pathogenic bacteria (Esherichia coli , Proteus mirabilis , Klebsiella sp , Pseudomonas aeruginosa , Serratia marcescens was studied . The combination of Probiotic and Prebiotics (Synbiotic) (Bifidobacterium sp + chicory ) and (Bifidobacterium sp + Inulin ) also tested for their antimicrobial activity against Pathogenic bacteria . Results showed that Bifidobacterium sp had good antimicrobial activity against all the Pathogenic bacteria tested , followed by chicory and inulin . The synergistic inhibitory effect of Synbiotic (Bifidobacterium sp + chicory ) and ( Bifidobacterium sp + inulin ) on Pathogenic bacteria was higher than the effect of Bifidobacterium sp alone, chicory alone and inulin alone.

Inhibitory Effect of a Hot Water Extract of Coffee “Silverskin” on Hyaluronidase

2011 ◽  
Vol 75 (6) ◽  
pp. 1205-1207 ◽  
Author(s):  
Mina FURUSAWA ◽  
Yusaku NARITA ◽  
Kazuya IWAI ◽  
Taiji FUKUNAGA ◽  
Osamu NAKAGIRI
Keyword(s):  
Water Extract ◽  
Inhibitory Effect ◽  
Hot Water ◽  
Coffee Silverskin ◽  
Hot Water Extract

scholarly journals Chemical Constituents of the Leaves of Campanula takesimana (Korean Bellflower) and Their Inhibitory Effects on LPS-induced PGE2 Production

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1232
Author(s):  
Yutong Qi ◽  
Se-In Choi ◽  
So-Ri Son ◽  
Hee-Soo Han ◽  
Hye Shin Ahn ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Hot Water ◽  
Pge2 Production ◽  
Raw 264.7 Cells ◽  
Inhibitory Effects ◽  
Spectrometric Data ◽  
Traditional Medicines ◽  
Hot Water Extract

Campanula takesimana Nakai (Campanulaceae; Korean bellflower) is one of the endemic herbs of Korea. The plant has been used as traditional medicines for treating asthma, tonsillitis, and sore throat in Korea. A hot water extract of the leaves of C. takesimana exhibited a significant inhibitory effect on lipopolysaccharide (LPS)-stimulated prostaglandin E2 (PGE2) production. Repetitive chromatographic separation of the hot water extract led to the isolation of three new neolignan glucosides, campanulalignans A–C (1–3), with 15 known compounds (4–18). The structures of new compounds 1–3 were elucidated by analyzing nuclear magnetic resonance (NMR) spectroscopic data, along with high resolution quadrupole time of flight mass (HR-Q-TOF-MS) spectrometric data. Among the isolates, simplidin (7), 5-hydroxyconiferaldehyde (11), icariside F2 (12), benzyl-α-l-arabinopyranosyl-(1″→6′)-β-d-glucopyranoside (13), and kaempferol 3-O-β-d-apiosyl (1→2)-β-d-glucopyranoside (15) were isolated from the Campanulaceae family for the first time. The isolates (1, 2, and 4–18) were assessed for their anti-inflammatory effects on LPS-stimulated PGE2 production on RAW 264.7 cells. 7R,8S-Dihydrodehydrodiconiferyl alcohol (5), 3′,4-O-dimethylcedrusin 9-O-β-glucopyranoside (6), pinoresinol di-O-β-d-glucoside (8), ferulic acid (10), 5-hydroxyconiferaldehyde (11), and quercetin (18) showed significant inhibitory effects on LPS-stimulated PGE2 production.

scholarly journals Anti-Obesity Effect of Hot Water Extract of Barley Sprout through the Inhibition of Adipocyte Differentiation and Growth

Metabolites ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 610
Author(s):  
Myeong-Jin Kim ◽  
Hye-Won Kawk ◽  
Sang-Hyeon Kim ◽  
Hy-Jae Lee ◽  
Ji-Won Seo ◽  
...  
Keyword(s):  
Adipocyte Differentiation ◽  
Morphological Changes ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Hot Water ◽  
Blood Biochemical ◽  
Hot Water Extract ◽  
Model Weight

Barley sprouts are known to have several effective physiological activities. In this study, the anti-obesity effect of a barley sprout hot water extract (BSE) was confirmed. Saponarin was quantitatively analyzed in BSE using HPLC, and the inhibitory effect on 3T3-L1 pre-adipocyte differentiation into adipocytes was confirmed by Oil Red O staining, TG assay, and Western blotting. In addition, the inhibitory effect of BSE on adipocyte growth was confirmed through glucose uptake and lipolysis of adipocytes. C57/BL/6N mice were induced to obesity with a high-fat diet, and BSE was administered to confirm the effect on an animal model. Weight gain, morphological changes in adipose tissue, changes in the food efficiency ratio, and blood biochemical changes were observed, and an improvement effect on fatty liver was confirmed. As a result, the anti-obesity effect of BSE was confirmed in vitro, and it was confirmed that this effect was also effective in vivo and that it could be helpful in the treatment of obesity-related diseases.

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