Effect of “Jian-Pi-Zhi-Dong Decoction” on Gamma-Aminobutyric Acid in a Mouse Model of Tourette Syndrome

The purpose of this study was to explore the positive effects of Jian-Pi-Zhi-Dong Decoction (JPZDD) on Tourette syndrome (TS) by investigating the expression of gamma-aminobutyric acid (GABA) and its type A receptor (GABAAR) in the striatum of a TS mice model. The model was induced by 3,3′-iminodipropionitrile (IDPN) treatment; then mice were divided into 4 groups (n=22, each); control and IDPN groups were gavaged with saline and the remaining 2 groups were gavaged with tiapride and JPZDD. We recorded the stereotypic behaviors of TS mice and measured the content of GABA in striatum by HPLC and GABAAR expression by immunohistochemistry and real-time PCR. Our results showed that JPZDD inhibited the abnormal behaviors of TS model mice and decreased GABA levels and GABAAR protein and mRNA expression in the striatum of TS model mice. In brief, the mechanism by which JPZDD alleviates TS symptoms may be associated with GABAAR expression downregulation in striatum which may regulate GABA metabolism.

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Gamma-aminobutyric acid-producing lactobacilli positively affect metabolism and depressive-like behaviour in a mouse model of metabolic syndrome

Scientific Reports ◽

10.1038/s41598-019-51781-x ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 5

Author(s):

E. Patterson ◽

P. M. Ryan ◽

N. Wiley ◽

I. Carafa ◽

E. Sherwin ◽

...

Keyword(s):

Metabolic Syndrome ◽

Mouse Model ◽

Plasma Cholesterol ◽

Forced Swim Test ◽

Lactobacillus Brevis ◽

Aminobutyric Acid ◽

Metabolic Dysfunction ◽

Gamma Aminobutyric Acid ◽

Gaba Production ◽

Glucose Challenge

Abstract Metabolic and neuroactive metabolite production represents one of the mechanisms through which the gut microbiota can impact health. One such metabolite, gamma-aminobutyric acid (GABA), can modulate glucose homeostasis and alter behavioural patterns in the host. We previously demonstrated that oral administration of GABA-producing Lactobacillus brevis DPC6108 has the potential to increase levels of circulating insulin in healthy rats. Therefore, the objective of this study was to assess the efficacy of endogenous microbial GABA production in improving metabolic and behavioural outcomes in a mouse model of metabolic dysfunction. Diet-induced obese and metabolically dysfunctional mice received one of two GABA-producing strains, L. brevis DPC6108 or L. brevis DSM32386, daily for 12 weeks. After 8 and 10 weeks of intervention, the behavioural and metabolic profiles of the mice were respectively assessed. Intervention with both L. brevis strains attenuated several abnormalities associated with metabolic dysfunction, causing a reduction in the accumulation of mesenteric adipose tissue, increased insulin secretion following glucose challenge, improved plasma cholesterol clearance and reduced despair-like behaviour and basal corticosterone production during the forced swim test. Taken together, this exploratory dataset indicates that intervention with GABA-producing lactobacilli has the potential to improve metabolic and depressive- like behavioural abnormalities associated with metabolic syndrome in mice.

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THE METABOLISM OF GAMMA AMINOBUTYRIC ACID IN THE LOBSTER NERVOUS SYSTEM

The Journal of Cell Biology ◽

10.1083/jcb.46.2.290 ◽

1970 ◽

Vol 46 (2) ◽

pp. 290-299 ◽

Cited By ~ 59

Author(s):

Z. W. Hall ◽

M. D. Bownds ◽

E. A. Kravitz

Keyword(s):

Aminobutyric Acid ◽

Inhibitory Neurons ◽

Decarboxylase Activity ◽

Succinic Semialdehyde ◽

Gamma Aminobutyric Acid ◽

Succinic Semialdehyde Dehydrogenase ◽

Gaba Metabolism ◽

Semialdehyde Dehydrogenase ◽

Electrophoretic Behavior ◽

Inhibitory Transmitter

γ-aminobutyric acid (GABA) is the inhibitory transmitter compound at the lobster neuromuscular junction. This paper presents a comparison of the enzymes of GABA metabolism in single identified inhibitory and excitatory axons from lobster walking legs. Inhibitory axons contain more than 100 times as much glutamic decarboxylase activity as do excitatory axons. GABA-glutamic transaminase is found in both excitatory and inhibitory axons, but about 50% more enzyme is present in inhibitory axons. The kinetic and electrophoretic behavior of the transaminase activity in excitatory and inhibitory axons is similar. Succinic semialdehyde dehydrogenase is found in both axon types, as is an unknown enzyme which converts a contaminant in radioactive glutamic acid to GABA. In lobster inhibitory neurons, therefore, the ability to accumulate GABA ultimately rests on the ability of the neuron to accumulate the enzyme glutamic decarboxylase.

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Synergistic antipruritic effects of gamma aminobutyric acid A and B agonists in a mouse model of atopic dermatitis

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2017.02.001 ◽

2017 ◽

Vol 140 (2) ◽

pp. 454-464.e2 ◽

Cited By ~ 15

Author(s):

Ferda Cevikbas ◽

Joao M. Braz ◽

Xidao Wang ◽

Carlos Solorzano ◽

Mathias Sulk ◽

...

Keyword(s):

Atopic Dermatitis ◽

Mouse Model ◽

Aminobutyric Acid ◽

Gamma Aminobutyric Acid

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Altered behavioral responses to gamma-aminobutyric acid pharmacological agents in a mouse model of Huntington's disease

Movement Disorders ◽

10.1002/mds.27107 ◽

2017 ◽

Vol 32 (11) ◽

pp. 1600-1609 ◽

Cited By ~ 8

Author(s):

Yi-Ting Hsu ◽

Ya-Gin Chang ◽

Ching-Pang Chang ◽

Jian-Jing Siew ◽

Hui-Mei Chen ◽

...

Keyword(s):

Mouse Model ◽

Huntington's Disease ◽

Huntington’S Disease ◽

Behavioral Responses ◽

Aminobutyric Acid ◽

Gamma Aminobutyric Acid ◽

Pharmacological Agents

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Effect of NC-1100 (1-(3,4-dimethoxyphenyl)-2-(4-diphenylmethylpiperazinyl) ethanol dihydrochloride) on .GAMMA.-aminobutyric acid(GABA) metabolism in rat brain: Analysis using stroke-prone spontaneously hypertensive rat.

The Japanese Journal of Pharmacology ◽

10.1254/jjp.50.131 ◽

1989 ◽

Vol 50 (2) ◽

pp. 131-139 ◽

Cited By ~ 3

Author(s):

Tsuneichi HASHIMOTO ◽

Misa KIMORI ◽

Yoshiko NAKAMURA ◽

Kinya KURIYAMA

Keyword(s):

Rat Brain ◽

Spontaneously Hypertensive Rat ◽

Aminobutyric Acid ◽

Gamma Aminobutyric Acid ◽

Spontaneously Hypertensive ◽

Gaba Metabolism ◽

Hypertensive Rat

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Gamma-aminobutyric acid (GABA) increases in vitro germ-tube formation and phospholipase B1 mRNA expression in Candida albicans

Mycoscience ◽

10.1007/s10267-011-0130-7 ◽

2012 ◽

Vol 53 (1) ◽

pp. 36-39 ◽

Cited By ~ 7

Author(s):

María Guadalupe Reyes-García ◽

Fernando García-Tamayo ◽

Francisca Hernández-Hernández

Keyword(s):

Candida Albicans ◽

Mrna Expression ◽

Germ Tube ◽

Tube Formation ◽

Aminobutyric Acid ◽

Gamma Aminobutyric Acid

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Chinese Medicine Formula “Jian-Pi-Zhi-Dong Decoction” Attenuates Tourette Syndrome via Downregulating the Expression of Dopamine Transporter in Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/385685 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

Dao-han Wang ◽

Wei Li ◽

Xiao-fang Liu ◽

Jin-ming Zhang ◽

Su-mei Wang

Keyword(s):

In Situ Hybridization ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Dopamine Transporter ◽

Tourette Syndrome ◽

Stereotyped Behavior ◽

Mice Model ◽

Chinese Medicine Formula ◽

Abnormal Behaviors

Jian-Pi-Zhi-Dong Decoction (JPZDD) is dedicated to the treatment for Tourette syndrome (TS) with the guidance of the theories of Traditional Chinese Medicine (TCM). This study aims to investigate the expression of dopamine transporter (DAT) in the striatum and stereotyped behavior of TS mice model by intervention of JPZDD. Mice were induced by 3,3′-iminodipropionitrile (IDPN, 350 mg kg−1day−1, i.p.) for 7 days and divided into 4 groups (n=20, each): control and IDPN groups were gavaged with saline and the remaining 2 groups with Tiapride (Tia, 50 mg kg−1day−1) and JPZDD (20 g kg−1day−1), respectively. The results showed that the scores of stereotyped behavior in IDPN+JPZDD group were significantly reduced. A noticeably increased11C-β-CFT binding at bilateral striatum was observed after administration of JPZDD versus that of IDPN or Tia. Immunohistochemistry and in situ hybridization studies manifested higher levels of DAT protein and mRNA in IDPN+JPZDD group. These findings not only demonstrated that JPZDD could effectively inhibit the abnormal behaviors of TS mice model, but also increase the level of DAT in striatum. Therefore, JPZDD could be one of potential treatments of patients with TS.

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