Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNA

Electrochemical behavior of the anticancer drug doxorubicin hydrochloride was studied using cyclic voltammetry in aqueous medium using Hepes buffer (pH~7.4). At this pH, doxorubicin hydrochloride undergoes a reversible two-electron reduction withE1/2value −665±5 mV (versus Ag/AgCl, saturated KCl). Depending on scan rates, processes were either quasireversible (at low scan rates) or near perfect reversible (at high scan rates). This difference in behavior of doxorubicin hydrochloride with scan rate studied over the same potential range speaks of differences in electron transfer processes in doxorubicin hydrochloride. Attempt was made to identify and understand the species involved using simulation. The information obtained was used to study the interaction of doxorubicin hydrochloride with calf thymus DNA. Cathodic peak current gradually decreased as more calf thymus DNA was added. The decrease in cathodic peak current was used to estimate the interaction of the drug with calf thymus DNA. Nonlinear curve fit analysis was applied to evaluate the intrinsic binding constant and site size of interaction that was compared with previous results on doxorubicin hydrochloride-DNA interaction monitored by cyclic voltammetry or spectroscopic techniques.

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Interaction of Calf Thymus DNA with the Ni(II) Complex of Sodium 1,4-Dihydroxy-9,10-Anthraquinone-2-Sulphonate: A Novel Method of Analysis Using Cyclic Voltammetry

International Journal of Electrochemistry ◽

10.1155/2012/183745 ◽

2012 ◽

Vol 2012 ◽

pp. 1-10 ◽

Cited By ~ 12

Author(s):

Partha Sarathi Guin ◽

Piyal Das ◽

Saurabh Das ◽

Parikshit Chandra Mandal

Keyword(s):

Cyclic Voltammetry ◽

Metal Ion ◽

Dna Interaction ◽

Calf Thymus Dna ◽

Reduction Peak ◽

Curve Fit ◽

Diffusion Controlled ◽

Binding Data ◽

Calf Thymus ◽

Electron Reduction

Hydroxy-9,10-anthraquinones are cheaper alternatives to anthracycline drugs. They closely resemble anthracycline drugs both from a structural and functional viewpoint. Electrochemical behavior of the Ni(II) complex (Na2[Ni(NaLH)2Cl2]⋅2H2O) of sodium 1,4-dihydroxy-9,10-anthraquinone-2-sulphonate (NaLH2), analogue of the core unit of anthracycline anticancer drugs, was studied at physiological pH using cyclic voltammetry. The Ni(II) complex of sodium 1,4-dihydroxy-9,10-anthraquinone-2-sulphonate undergoes diffusion-controlled one-electron reduction that enables performing an electrochemical study on the interaction of the complex with calf thymus DNA. The complex was titrated with increasing concentrations of DNA, and the reduction peak for the unbound complex helped in evaluating binding parameters. Analysis of binding data using nonlinear curve fit in a cyclic voltammetry experiment is the first such attempt. The paper evaluates site size of interaction that also serves as a means to determine stoichiometry of complex formation, between a metal ion and ligand from a DNA interaction study, probably a first of its kind.

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A steady-state and time-resolved fluorescence study on liposome-calf thymus DNA interaction: probed by an anticancer drug ellipticine

RSC Advances ◽

10.1039/c3ra43037e ◽

2013 ◽

Vol 3 (42) ◽

pp. 19572 ◽

Cited By ~ 8

Author(s):

Anupam Das ◽

Raina Thakur ◽

Anjan Chakraborty

Keyword(s):

Steady State ◽

Anticancer Drug ◽

Dna Interaction ◽

Calf Thymus Dna ◽

Time Resolved Fluorescence ◽

Fluorescence Study ◽

Time Resolved ◽

Calf Thymus

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Studies on free radical scavenging, cancer cell antiproliferation, and calf thymus DNA interaction of Schiff bases

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2017.05.020 ◽

2017 ◽

Vol 172 ◽

pp. 129-138 ◽

Cited By ~ 20

Author(s):

Cleiton M. da Silva ◽

Marina M. Silva ◽

Fabiano S. Reis ◽

Ana Lúcia T.G. Ruiz ◽

João E. de Carvalho ◽

...

Keyword(s):

Free Radical ◽

Schiff Bases ◽

Cancer Cell ◽

Radical Scavenging ◽

Dna Interaction ◽

Free Radical Scavenging ◽

Calf Thymus Dna ◽

Calf Thymus

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Synthesis and Characterisation of Copper(II) Complexes with Tridentate NNO Functionalized Ligand: Density Function Theory Study, DNA Binding Mechanism, Optical Properties, and Biological Application

Bioinorganic Chemistry and Applications ◽

10.1155/2014/104046 ◽

2014 ◽

Vol 2014 ◽

pp. 1-13 ◽

Cited By ~ 15

Author(s):

Madhumita Hazra ◽

Tanushree Dolai ◽

Akhil Pandey ◽

Subrata Kumar Dey ◽

Animesh Patra

Keyword(s):

Density Function ◽

Antimicrobial Agents ◽

Function Theory ◽

Free Ligand ◽

Fluorescence Emission ◽

Building Blocks ◽

Density Function Theory ◽

Calf Thymus Dna ◽

Spectroscopic Techniques ◽

Calf Thymus

The photo physical properties of two mononuclear pentacoordinated copper(II) complexes formulated as [Cu(L)(Cl)(H2O)] (1) and [Cu(L)(Br)(H2O)] (2)HL = (1-[(3-methyl-pyridine-2-ylimino)-methyl]-naphthalen-2-ol) were synthesized and characterized by elemental, physicochemical, and spectroscopic methods. The density function theory calculations are used to investigate the electronic structures and the electronic properties of ligand and complex. The interactions of copper(II) complexes towards calf thymus DNA were examined with the help of absorption, viscosity, and fluorescence spectroscopic techniques at pH 7.40. All spectroscopy's result indicates that complexes show good binding activity to calf thymus DNA through groove binding. The optical absorption and fluorescence emission properties of microwires were characterized by fluorescence microscope. From a spectroscopic viewpoint, all compounds strongly emit green light in the solid state. The microscopy investigation suggested that microwires exhibited optical waveguide behaviour which are applicable as fluorescent nanomaterials and can be used as building blocks for miniaturized photonic devices. Antibacterial study reveals that complexes are better antimicrobial agents than free Schiff base due to bacterial cell penetration by chelation. Moreover, the antioxidant study of the ligand and complexes is evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical assays, which demonstrate that the complexes are of higher antioxidant activity than free ligand.

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