scholarly journals The Development of Biomimetic Spherical Hydroxyapatite/Polyamide 66 Biocomposites as Bone Repair Materials

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Xuesong Zhang ◽  
Ming Lu ◽  
Yan Wang ◽  
Xiaojing Su ◽  
Xuelian Zhang
Keyword(s):  
Bone Repair ◽  
Chemical Bonds ◽  
Polyamide 66 ◽  
Biomedical Material ◽  
Natural Bone ◽  
Repair Materials ◽  
Proliferation And Differentiation ◽  
Molecule Interactions ◽  
Bone Repair Materials

A novel biomedical material composed of spherical hydroxyapatite (s-HA) and polyamide 66 (PA) biocomposite (s-HA/PA) was prepared, and its composition, mechanical properties, and cytocompatibility were characterized and evaluated. The results showed that HA distributed uniformly in the s-HA/PA matrix. Strong molecule interactions and chemical bonds were presented between the s-HA and PA in the composites confirmed by IR and XRD. The composite had excellent compressive strength in the range between 95 and 132 MPa, close to that of natural bone.In vitroexperiments showed the s-HA/PA composite could improve cell growth, proliferation, and differentiation. Therefore, the developed s-HA/PA composites in this study might be used for tissue engineering and bone repair.

scholarly journals Strontium-substituted sub-micron bioactive glasses inhibit ostoclastogenesis through suppression of RANKL-induced signaling pathway

2020 ◽  
Vol 7 (3) ◽  
pp. 303-311 ◽  
Author(s):  
Deqiu Huang ◽  
Fujian Zhao ◽  
Wendong Gao ◽  
Xiaofeng Chen ◽  
Zhouyi Guo ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Signaling Pathway ◽  
Bone Repair ◽  
Superior Performance ◽  
Bioactive Glasses ◽  
Repair Materials ◽  
Potential Impact ◽  
Bone Repair Materials

Abstract Strontium-substituted bioactive glass (Sr-BG) has shown superior performance in bone regeneration. Sr-BG-induced osteogenesis has been extensively studied; however, Sr-BG-mediated osteoclastogenesis and the underlying molecular mechanism remain unclear. It is recognized that the balance of osteogenesis and osteoclastogenesis is closely related to bone repair, and the receptor activators of nuclear factor kappaB ligand (RANKL) signaling pathway plays a key role of in the regulation of osteoclastogenesis. Herein, we studied the potential impact and underling mechanism of strontium-substituted sub-micron bioactive glass (Sr-SBG) on RANKL-induced osteoclast activation and differentiation in vitro. As expected, Sr-SBG inhibited RANKL-mediated osteoclastogenesis significantly with the experimental performance of decreased mature osteoclasts formation and downregulation of osteoclastogenesis-related gene expression. Furthermore, it was found that Sr-SBG might suppress osteoclastogenesis by the combined effect of strontium and silicon released through inhibition of RANKL-induced activation of p38 and NF-κB pathway. These results elaborated the effect of Sr-SBG-based materials on osteoclastogenesis through RANKL-induced downstream pathway and might represent a significant guidance for designing better bone repair materials.

scholarly journals The Preparation andIn VitroEvaluations of a Nanoscaled Injectable Bone Repair Material

2015 ◽  
Vol 2015 ◽  
pp. 1-8
Author(s):  
Xinhui Liu ◽  
Chao Zhu ◽  
Yijiong Li ◽  
Yueling Yan ◽  
Chuanyong Hou ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Bone Repair ◽  
Cell Attachment ◽  
Repair Material ◽  
Cellular Functions ◽  
Repair Materials ◽  
Proliferation And Differentiation ◽  
Negative Effect ◽  
Bone Repair Material

There are usually two forms of bone repair materials, block and granular, for common clinical use. This paper describes a novel injectable material, nano-HA/collagen/alginate (nHAC/Alg) composite biomaterial, including its preparation and evaluationsin vitro. Based on the idea of bionics and the study of collagen/calcium phosphate salt composite materials, the injectable bone repair material was developed. Then, human bone marrow stem cells (hBMSCs) were cultured on the nHAC/Alg material. The cell attachment, proliferation, and differentiation were evaluated with inverted microscope, scanning electron microscope, laser scanning confocal microscope, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) analysis, and alkaline phosphatase (ALP) test. The results showed that nHAC/Alg not only had no negative effect on cellular functions but also promotes cell proliferation and differentiation into osteogenic cells, which suggests that the nanoscaled injectable bone repair material has good clinical application prospects for bone repair.

In vitro evaluation of freeze-drying chitosan-mineralized collagen/Mg–Ca alloy composites for osteogenesis

2022 ◽  
pp. 088532822110492
Author(s):  
Zhenbao Zhang ◽  
Xirao Sun ◽  
Jingxin Yang ◽  
Chengyue Wang
Keyword(s):  
Corrosion Resistance ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Matrix Mineralization ◽  
Repair Materials ◽  
Micro Arc Oxidation ◽  
Umbilical Vein Endothelial Cells ◽  
Endothelial Growth Factor ◽  
Mineralized Collagen

Magnesium (Mg) alloy with good mechanical properties and biodegradability is considered as one of the ideal bone repair materials. However, the rapid corrosion of Mg-based metals can pose harm to the function of an implant in clinical applications. In this study, micro-arc oxidation coating was prepared on the surface of the Mg–Ca matrix, then the chitosan and mineralized collagen (nano-hydroxyapatite/collagen; nHAC) were immobilized on the surface of the MAO/Mg–Ca matrix to construct the CS-nHAC/Mg–Ca composites of different component proportions (the ratio of CS to nHAC is 2:1, 1:1, and 1:2, respectively). The corrosion resistance, osteogenic activity, and angiogenic ability were extensively investigated. The results indicated that the CS-nHAC reinforcement materials can improve the corrosion resistance of the Mg matrix significantly and promote the proliferation and adhesion of mouse embryo osteoblast precursor cells (MC3T3-E1) and human umbilical vein endothelial cells (HUVECs). In addition, the CS-nHAC/Mg–Ca composites can not only promote the alkaline phosphatase (ALP) activity and extracellular matrix mineralization of MC3T3-E1 cells but also enhance the migration motility and vascular endothelial growth factor (VEGF) expression of HUVECs. Meanwhile, the 2CS-1nHAC/Mg–Ca composite exhibited the optimum function characteristics compared with other samples. Therefore, considering the improvement of corrosion resistance and biocompatibility, the CS-nHAC/Mg–Ca composites are expected to be a promising orthopedic implant.

Peptide‐modified bone repair materials: Factors influencing osteogenic activity

2019 ◽  
Vol 107 (7) ◽  
pp. 1491-1512 ◽  
Author(s):  
Jie Liao ◽  
Shuai Wu ◽  
Kun Li ◽  
Yubo Fan ◽  
Nicholas Dunne ◽  
...  
Keyword(s):  
Bone Repair ◽  
Osteogenic Activity ◽  
Factors Influencing ◽  
Repair Materials ◽  
Bone Repair Materials

Self-assembly of pifithrin-α-loaded layered double hydroxide/chitosan nanohybrid composites as a drug delivery system for bone repair materials

2017 ◽  
Vol 5 (12) ◽  
pp. 2245-2253 ◽  
Author(s):  
Yi-Xuan Chen ◽  
Rong Zhu ◽  
Zheng-liang Xu ◽  
Qin-Fei Ke ◽  
Chang-Qing Zhang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Layered Double Hydroxide ◽  
Delivery System ◽  
Self Assembly ◽  
Bone Repair ◽  
Repair Materials ◽  
Double Hydroxide ◽  
First Time ◽  
Bone Repair Materials

The self-assembly of pifithrin-α-loaded layered double hydroxide/chitosan nanohybrid composites as a drug delivery system was demonstrated for the first time to improve the cytocompatibility and enhance the osteoinductivity for the treatment of bone defects.

Effect of Strontium Enhanced Calcium Phosphate Coating on In Vitro Behavior of Human Mesenchymal Stem Cell (hMSC)

2014 ◽  
Vol 21 ◽  
pp. 35-44
Author(s):  
Samuel C. Uzoechi ◽  
Goddy C. Okoye ◽  
Kennedy O. Ejeta ◽  
Benjamin I. Nkem ◽  
Gideon I. Ndubuka
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Mineral ◽  
Bone Repair ◽  
Human Mesenchymal Stem Cell ◽  
Calcium Phosphate Coating ◽  
Proliferation And Differentiation ◽  
Biomimetic Coating ◽  
The Fourier Transform

Calcium phosphate is a widely used material as coating for metallic implants. This research describes a biomimetic coating techniques based on deposition of calcium phosphate films on a Ti6Al4V plates that was used to study the effect of strontium additive on the behavior of hMSCs. In this study, strontium additive was homogenously deposited onto calcium phosphate films on a Ti6AlV plates by using a biomimetic techniques. Strontium affected composition and morphology of calcium phosphate deposited on a Ti6Al4V plates to a varying degree, according to concentration of solutions used. The effect of strontium additive on proliferation and differentiation of hMSCs depended on the solution and concentration tested. In general, all individual three coatings showed decreased hMSCs proliferation. Strontium additive demonstrated a significant increase in differentiation into osteogenic lineage when compared with the control and calcium phosphate films without strontium additive. However, no cytotoxic effect of strontium additive in the concentrations tested was detected. The Fourier transform infrared spectra showed that this new coating closely resembles bone mineral. The techniques illustrated in this study mimics bone mineral containing strontium additive, making it constructive for studying basic processes of in vitro bone formation. The results showed in this study can be used for changing bone graft substitutes by addition of strontium additive on implants in order to affect their performance in bone repair and regeneration. Also, the system can aid rapid bone formation around the implant, reducing therewith the patient’s recovery time after surgery.

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