scholarly journals Assessment of Free Radical Scavenging Potential and Oxidative DNA Damage Preventive Activity ofTrachyspermum ammiL. (Carom) andFoeniculum vulgareMill. (Fennel) Seed Extracts

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Nandini Goswami ◽  
Sreemoyee Chatterjee
Keyword(s):  
Dna Damage ◽  
Free Radicals ◽  
Free Radical ◽  
Oxidative Dna Damage ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Dpph Radical ◽  
Preventive Activity ◽  
Dpph Radical Scavenging ◽  
Seed Extracts

Oxidation of biomolecules such as carbohydrates, proteins, lipids, and nucleic acids results in generation of free radicals in an organism which is the major cause of onset of various degenerative diseases. Antioxidants scavenge these free radicals, thereby protecting the cell from damage. The present study was designed to examine the free radical scavenging potential and oxidative DNA damage preventive activity of traditionally used spicesTrachyspermum ammiL. (carom) andFoeniculum vulgareMill. (fennel). The aqueous, methanolic, and acetonic extracts ofT. ammiandF. vulgareseeds were prepared using soxhlet extraction assembly and subjected to qualitative and quantitative estimation of phytochemical constituents. Free radical scavenging potential was investigated using standard methods, namely, DPPH radical scavenging assay and ferric reducing antioxidant power assay along with the protection against oxidative DNA damage. The results stated that acetonic seed extracts (AAcSE and FAcSE) of both the spices possessed comparatively high amount of total phenolics whereas methanolic seed extracts (AMSE and FMSE) were found to have highest amount of total flavonoids. At 1 mg/mL concentration, highest DPPH radical scavenging activity was shown by FMSE (96.2%), AAcSE was recorded with highest FRAP value (2270.27 ± 0.005 μmol/L), and all the seed extracts have been shown to mitigate the damage induced by Fenton reaction on calf thymus DNA. Therefore, the study suggests thatT. ammiandF. vulgareseed extracts could contribute as a highly significant bioresource of antioxidants to be used in our day-to-day life and in food and pharmaceutical industry.

scholarly journals Comparison of Antioxidant Activity and Free Radical Scavenging Activity of Gardenia Jasminoides with Three Different Regional Sources (P17-005-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Xiangqin Zhou ◽  
Hyun-Sook Kim
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Mass ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Abstract Objectives To compare the antioxidant activity and free radical scavenging activity of three different regional sources of Gardenia Jasminoides Methods Gardenia Jasminoides grown in multiple regions in Asia (mainly in China and Korea) was commonly used as a natural yellow dye, and has been one of the popular traditional Chinese medicines since the discovery of its biological property a few decades ago. In this experiment, three different samples were used; GJ dry mass powder from South Korea (GK), GJ dry mass powder from China (GC), GJ extract powder(extracted with 50% ethanol and ended by dry freeze process) from dry mass powder from South Korea. (GE). To investigate the antioxidant activities, we employed the following parameters: DPPH radical scavenging activity, ABTS radical scavenging activity, Total polyphenol content, and Total flavonoid content. Total polyphenol content was based on gallic acid standard curve and total flavonoid content was measured using quercetin as standard. The DPPH radical scavenging activity was experimented using 1 mM DPPH methanol standard solution (the solution standardized in O.D value of 0.95∼0.99 at 525 nm), and the ABTS scavenging activity was carried out using ABTS standardradical stock solution (the solution standardized in the O.D. value 0.7 ± 0.02 at 734 nm). Results The DPPH radical scavenging activity is highest in ethanol extract powder (88.8%) and lowest in dry mass China powder (65.5%). The ABTS radical scavenging activity is highest in ethanol extract powder (100.2%) and lowest in dry mass China powder (98.4%) with no significant differences among samples. In total polyphenol contents assay, China dry mass powder showed the highest contents of 3.0 g GAE/100 g, and the lowest contents of 1.5 g GAE/100 g dry mass powder(Korea) (GAE: gallic acid equivalent). In total flavonoid contents assay, the highest content is in China dry mass powder with 35.4 g QE/100 g dry mass powder, and the lowest content was observed in domestic South Korea powder of 10.2 g QE/100 g dry mass powder (QE: quercetin equivalent). Conclusions Although China dry mass GJ powder showed the highest polyphenol and flavonoids contents, the free radical scavenging activity was observed highest in ethanol extract powder of GJ. Thus we may suggest that the extraction method would be a powerful way to get improved antioxidant activity and free radical scavenging activity of the raw GJ products according to the purpose of study. Funding Sources Department of Food and Nutrition, Sookmyung Women's University, Republic of Korea.

Free radical scavenging capacity and protective effect ofBacopa monniera L. on DNA damage

2003 ◽  
Vol 17 (8) ◽  
pp. 870-875 ◽  
Author(s):  
Alessandra Russo ◽  
Angelo A. Izzo ◽  
Francesca Borrelli ◽  
Marcella Renis ◽  
Angelo Vanella
Keyword(s):  
Dna Damage ◽  
Free Radical ◽  
Protective Effect ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Analysis of Free-Radical Scavenging of Yerba Mate (Ilex paraguriensis) using Electron Spin Resonance and Radical-Induced DNA Damage

2010 ◽  
Vol 75 (1) ◽  
pp. C14-C20 ◽  
Author(s):  
Stephen S. Leonard ◽  
Vallie J. Hogans ◽  
Zulema Coppes-Petricorena ◽  
Cody J. Peer ◽  
Timothy A. Vining ◽  
...  
Keyword(s):  
Dna Damage ◽  
Electron Spin Resonance ◽  
Free Radical ◽  
Electron Spin ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Yerba Mate ◽  
Spin Resonance

scholarly journals Modulation of rabbit muscle sarcoplasmic reticulum Ca2+-ATPase activity by novel quercetin derivatives

2013 ◽  
Vol 6 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Dušan Blaškovič ◽  
Petronela Žižková ◽  
Filip Držík ◽  
Jana Viskupičová ◽  
Miroslav Veverka ◽  
...  
Keyword(s):  
Heart Failure ◽  
Free Radicals ◽  
Free Radical ◽  
Sarcoplasmic Reticulum ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rabbit Muscle ◽  
Scavenging Activity ◽  
Quercetin Derivatives

Abstract Sarcoplasmic reticulum Ca2+-ATPase (SERCA) is the pump crucial for calcium homeostasis and its impairment results in pathologies such as myopathy, heart failure or diabetes. Modulation of SERCA activity may represent an approach to the therapy of diseases with SERCA impairment involvment. Quercetin is flavonoid known to modulate SERCA activity. We examined the effect of nine novel quercetin derivatives on the activity of the pump. We found that 5-morpholinohydroxypoxyquercetin, di(prenylferuoyl)quercetin, di(diacetylcaffeoyl)-mono-(monoacetylcaffeoyl)quercetin and monoacetylferuloylquercetin stimulated the activity of SERCA. On the contrary, monochloropivaloylquercetin, tri(chloropivaloyl)quercetin, pentaacetylquercetin, tri(trimethylgalloyl)quercetin and diquercetin inhibited the activity of the pump. To identify compounds with a potential to protect SERCA against free radicals, we assessed the free radical scavenging activity of quercetin derivatives. We also related lipophilicity, an index of the ability to incorporate into the membrane of sarcoplasmic reticulum, to the modulatury effect of quercetin derivatives on SERCA activity. In addition to its ability to stimulate SERCA, di(prenylferuloyl)quercetin showed excellent radical scavenging activity.

Melatonin as a major skin protectant: from free radical scavenging to DNA damage repair

2008 ◽  
Vol 17 (9) ◽  
pp. 713-730 ◽  
Author(s):  
Tobias W. Fischer ◽  
Andrzej Slominski ◽  
Michal A. Zmijewski ◽  
Russel J. Reiter ◽  
Ralf Paus
Keyword(s):  
Dna Damage ◽  
Free Radical ◽  
Dna Damage Repair ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Damage Repair

scholarly journals Phytochemical screening, free radical scavenging, antioxidant activity and phenolic content of Dodonaea viscosa

2012 ◽  
Vol 77 (4) ◽  
pp. 423-435 ◽  
Author(s):  
Tauheeda Riaz ◽  
Athar Abbasi ◽  
A Aziz-Ur-Rehman ◽  
Tayyaba Shahzadi ◽  
Muhammad Ajaib ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Antioxidant Activity ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dodonaea Viscosa ◽  
Total Antioxidant

The purpose of this study was to evaluate the antioxidant potential of Dodonaea viscosa Jacq. Methanolic extract of the plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and nbutanol sequentially. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol fraction. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. The results revealed that ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 81.14 ? 1.38% inhibition of DPPH radical at a concentration of 60 ?g/ml. The IC50 of this fraction was found to be 33.95 ? 0.58 ?g/ml, relative to butylated hydroxytoluene (BHT), having IC50 of 12.54 ? 0.89 ?g/mL. It also showed highest FRAP value (380.53 ? 0.74 ?M of trolox equivalents) as well as highest total phenolic contents (208.58 ? 1.83 GAE ?g/g) and highest value of inhibition of lipid peroxidation (58.11 ? 1.49% at concentration of 500 ?g/ml) as compared to the other studied fractions. The chloroform fraction showed highest total antioxidant activity i.e.1.078 ? 0.59 (eq. to BHT).

scholarly journals Evaluation of Free Radical Scavenging Activity in Ethanolic Extract from Promising Accessions of Curcuma aeruginosa RoxB.

Molekul ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. 133 ◽  
Author(s):  
Waras Nurcholis ◽  
Nurul Khumaida ◽  
Muhamad Syukur ◽  
Maria Bintang
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Pharmaceutical Products ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Ic50 Values

This study evaluated the free radical scavenging activity in ethanolic extracts from 20 accessions of Curcuma aeruginosa. The radical scavenging activity of the extract accessions was investigated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. IC50 values for DPPH radical scavenging activity ranged from 89.81 to 505.65 µg mL-1. Based on IC50 values, twenty accessions of C. aeruginosa can be divided into three groups: strong (two accessions); moderate (seventeen accessions); and low (one accession) of DPPH scavenger. Sukoharjo (SH) and Muara Bungo (MB) showed promising accessions for antioxidant potential, thus these accessions important to selection for future breeding program in pharmaceutical products.

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