scholarly journals Modulation of rabbit muscle sarcoplasmic reticulum Ca2+-ATPase activity by novel quercetin derivatives

2013 ◽  
Vol 6 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Dušan Blaškovič ◽  
Petronela Žižková ◽  
Filip Držík ◽  
Jana Viskupičová ◽  
Miroslav Veverka ◽  
...  
Keyword(s):  
Heart Failure ◽  
Free Radicals ◽  
Free Radical ◽  
Sarcoplasmic Reticulum ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rabbit Muscle ◽  
Scavenging Activity ◽  
Quercetin Derivatives

Abstract Sarcoplasmic reticulum Ca2+-ATPase (SERCA) is the pump crucial for calcium homeostasis and its impairment results in pathologies such as myopathy, heart failure or diabetes. Modulation of SERCA activity may represent an approach to the therapy of diseases with SERCA impairment involvment. Quercetin is flavonoid known to modulate SERCA activity. We examined the effect of nine novel quercetin derivatives on the activity of the pump. We found that 5-morpholinohydroxypoxyquercetin, di(prenylferuoyl)quercetin, di(diacetylcaffeoyl)-mono-(monoacetylcaffeoyl)quercetin and monoacetylferuloylquercetin stimulated the activity of SERCA. On the contrary, monochloropivaloylquercetin, tri(chloropivaloyl)quercetin, pentaacetylquercetin, tri(trimethylgalloyl)quercetin and diquercetin inhibited the activity of the pump. To identify compounds with a potential to protect SERCA against free radicals, we assessed the free radical scavenging activity of quercetin derivatives. We also related lipophilicity, an index of the ability to incorporate into the membrane of sarcoplasmic reticulum, to the modulatury effect of quercetin derivatives on SERCA activity. In addition to its ability to stimulate SERCA, di(prenylferuloyl)quercetin showed excellent radical scavenging activity.

Free radicals scavenging aptitude of Spilanthes acmella

2021 ◽  
pp. 2631-2634
Author(s):  
K. Gouri Sankar ◽  
B. S. Venkateswarlu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Bioactive Molecules ◽  
Scavenging Activity ◽  
Spilanthes Acmella

Now a days, oxidative stress is one of the major disease around the world. The excess production of oxidants in the body are key source for development of different chronic diseases and are leading to damage the multiple organs' function, finally to cellular mortality. The natural remedy is the most important medicine to control the overproduction of oxidants and to obtain more antioxidants. Natural product have been using to treat diseases and as food supplements as nutrients. In this point of view, the current study was carried to evaluate the antioxidant activity of Spilanthes acmella root parts. The free radical scavenging activity was studied against 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide and hydroxyl free radicals using standard methods. The Spilanthes acmella root part extracts showed concentration dependent antioxidant activity on tested free radicals. The extracts showed modest effect on free radicals compared to the standard drug ascorbic acid and hydro-alcoholic extract possesses more activity than other two extracts. The end results of current study clearly state that Spilanthes acmella root possess free radical scavenging activity and also confirms its' traditional value in traditional medicine. The further research is worthful on isolation of different bioactive molecules and different biological activities evaluation on Spilanthes acmella.

scholarly journals Study of in vitro and in vivo free radical scavenging activity for radioprotection of cerium oxide nanoparticles

2018 ◽  
Vol 15 (35) ◽  
pp. 40-47
Author(s):  
Asia H. Al-Mashhadani
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Scavenging Activity

Nanoceria have shown numerous unique characteristics, such as biocompatibility and are excellent agents for biological applications. The aim of this study is to investigate cerium oxide nanoparticles for 2, 2- diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging activity and their ability to offer protection against ionizing radiation. In vitro antioxidant activity study of nanoceria particles has shown good free radical scavenging activity for DPPH radical assayed within a concentration range of 0.01 to 0.05 g/l, at higher concentrations of nanoparticles showed reverse trend in absorbance and inhibition indicating this finite rang of concentration is suitable for scavenging free radicals, also nanoparticles were found to have significant antioxidant capacity and thus can be used as potential radical scavenger against deleterious damages caused by the free radicals. The results of histopathological examination showed effectiveness of CeO2 nanoparticles in radioprotection of skin cells in animal models during radiation exposure.

scholarly journals Inhibition of free radical activity by dual PPAR α and PPAR γ agonist using analytical assay methods

2017 ◽  
Vol 6 (7) ◽  
pp. 1670
Author(s):  
Vijaya Kumar A. E. ◽  
Vinay M. ◽  
Seethalakshami S.
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Peroxisome Proliferator ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Ppar Γ

Background: Overproduction of free radicals involved in the pathology of a wide variety of clinical disorders. Poor glycaemic control in diabetic people leads to free radical production responsible for diabetic related complications. Antioxidants produces resistance against the oxidative stress by scavenging free radicals may useful in treating diabetic related complications. Saroglitazar is a newer antidiabetic drug act on dual Peroxisome Proliferator Receptor Agonist α (PPAR α) and PPAR γ agonist with protection effect on Diabetes mellitus induced lipid dystrophy. Our study was done to evaluate the in vitro antioxidant effect Saroglitazar by 1, 1 Diphenyl 2 Picryl hydrazide (DPPH) and Nitric Oxide (NO) method.Methods: In this study, we demonstrated invitro antioxidant activity by using 10 mg/dl stock solutions of Saroglitazar. DPPH and NO free radical scavenging test were done for different concentration of Saroglitazar.Results: Saroglitazar showed concentration dependent free radical scavenging activity in DPPH assay. In DPPH assay at higher concentration 1000ug concentration showed 49.18% free radical scavenging activity. At lower concentration 10ug showed 17.18% free radical scavenging activity. NO scavenging activity at lower concentration 100ug showed 55.15% activity. But the higher concentration (1000ug) only slight increase in 60.15% activity.Conclusions: Thus Saroglitazar invitro antioxidant analysis proved that it is a potent antioxidant.

scholarly journals Free Radical Scavenging Activity and Antioxidants of Hydrocotyle vulgaris L. (Pennywort): Baseline Study in Developing Biocosmetic-Antidote for Pathological Aging

2018 ◽  
Vol 10 ◽  
pp. 1-12
Author(s):  
Romnick Ureta ◽  
Siegfred Mejico ◽  
Yvonne Maranan
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Pathological Aging ◽  
Vis Spectroscopy

Pathological aging due to harmful free radicals and oxidative stress has been a serious threat to human health (Kensella & He, 2008). Thus, the study aimed to evaluate the free radical scavenging activity, antioxidants, essential vitamins, and physicochemical properties of Hydrocotyle vulgaris L. (Pennywort) which could serve as basis in developing a biocosmetic antidote for pathological aging. H.vulgaris L. plant was harvested, air dried and then extracted via soxhlet. UV-vis spectroscopy was used for scavenging activity and antioxidants of the plant while High Performance Liquid Chromatography (HPLC) was applied for the identification of essential vitamins. Phytochemicals, pH and spreadability values of the plant were also tested. Results revealed that H.vulgaris L. has an abundant presence of flavonoid and alkaloid while traced presence of tannin. Good trend for scavenging activity was also observed exhibiting low absorbance of possible free radicals with IC50 of 29.75 and antioxidant activity of 158.13 (Total Phenolics as gallic acid in %w/w). Meanwhile, β-carotene (10.4 mg/kg), Riboflavin (4.08mg/kg), and Vitamins C (70.2mg/kg) and E (26.9mg/kg) were also found in the plant. Non-irritating pH levels of 5.7 and 7.14 for 50% and 75% concentrations of the extracts with good spreadability value of 17.51 g∙cm/sec (for 75% concentration) were also obtained. Based on the findings of the study, Pennywort has a very promising therapeutic characteristics with its good scavenging activity and antioxidants. Thus, the development of a biocosmetic product that could serve as an antidote for pathological aging from Pennywort appeared to be very apparent.

Assessment of free radical scavenging activity and polyphenolic content in leaves of Neurocalyx calycinus (R. Br. ex Benn.) Rob. - a medicinal plant used by Cholanaickan tribe

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Ajayakumar Lalitha Aneeshkumar
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Dried Extract

Neurocalyx calycinus (R. Br. ex Benn.) Rob. is an endemic species of the family Rubiaceae, found in Western Ghats of India and Sri Lanka. The leaf paste of N. calycinus has been used by Cholanaickan tribe as medicine to heal wounds, burns, inflammation and pain. Many of the human diseases are manifested due to the accumulation of free radicals. Plant derived antioxidants have the power to scavenge these free radicals without any internal complications. Aqueous, n-hexane, chloroform, ethyl acetate and ethanol extracts of the leaves of N. calycinus were selected for qualitative phytochemical screening. DPPH, nitric oxide, superoxide and hydroxyl radical scavenging assays were carried out. Total phenolic, flavonoid and antioxidant content of all the extract was measured. Phytochemical screening showed that alkaloids, steroids, phenolic compounds, glycosides and saponins were found in the ethanolic extract. Ethanol extract showed better antioxidant activity than the other solvent extracts. The reducing power of all the extracts was increased dose dependently. Highest amount of phenolic (56.27 mg of gallic acid equivalent /gm of dried extract), flavonoid (36.92 mg of quercetin equivalent/gm of dried extract) and antioxidant (85.68 mg of ascorbic acid equivalent/gm of dried extract) content was found in the ethanolic extract. A positive correlation (R2) was found between the total phenolic content and free radical scavenging activity of the leaf extract of N. calycinus. The results confirmed that the phenolic compounds in the ethanolic extract of the leaves of N. calycinus are potent source of natural antioxidants.

scholarly journals Free Radical Scavenging Activity, Determination of Phenolic Compounds and HPLC-DAD/ESIMS Profile of Campomanesia Adamantium Leaves

2011 ◽  
Vol 6 (7) ◽  
pp. 1934578X1100600
Author(s):  
Aislan C.R.F. Pascoal ◽  
Carlos Augusto Ehrenfried ◽  
Marcos N. Eberlin ◽  
Maria Élida Alves Stefanello ◽  
Marcos José Salvador
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Numerous diseases are induced by free radicals via lipid peroxidation, protein peroxidation and DNA damage. It has been known that a variety of plant extracts have antioxidant activity to scavenge free radicals. Campomanesia adamantium (Myrtaceae) is a small tree with edible fruit, commonly known as “guavira” or “guabiroba-branca” that has been used in popular medicine as depurative anti-diarrhoeic, anti-inflammatory, anti-rheumatic and to liver diseases. In this study, the antiradical activities of ethanol crude extract of the leaves from C. adamantium and the ethyl acetate and butanol fractions obtained by partition, were determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl radical) and ORAC-FL (Oxygen Radical Absorbance Capacity) assays. The total phenol content in the samples was estimated by Folin Ciocalteau method (FCR). In an initial evaluation the ethanolic extract and the fractions ethyl acetate and butanol have shown levels of phenolic compounds between 15- 74 mg GAE/g in FCR assay, showed DPPH free-radical scavenging activity with SC50 in the range of 7.77-13.35 μg/mL and demonstrated antioxidant capacity between 2648-3502 μmol TE/g of extract and fractions in the ORAC-FL assay. HPLC-DAD and ESIMS analysis revealed were that the extract of the leaves of C. adamantium studied appears to contain flavonoids as major constituents, including isoquercetrin and quercetin that exhibit proven antioxidant activity.

scholarly journals Free Radical Scavenging Activity of Infusions of Different Medicinal Plants for Use in Obstetrics

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2016
Author(s):  
Sylwia Jarco ◽  
Barbara Pilawa ◽  
Paweł Ramos
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Plant Materials ◽  
Eupatorium Cannabinum ◽  
Uva Radiation

An X-band (9.3 GHz) electron paramagnetic resonance (EPR) spectroscopy was used to examine the free radical scavenging activity of the following infusions, which were nonirradiated and exposed to UVA: root of Asparagus racemosus and herbs of Mitchella repens, Cnicus benedictus L., Galega officinalis L., and Eupatorium cannabinum L. The plant materials for obstetrics applications were chosen for analysis. The aims of these studies were to compare the free radical scavenging ability of the tested infusions and to determine the influence of UVA irradiation of the plant materials on interactions of these infusions with free radicals. Both the magnitude and kinetics of the interactions of the infusions with the model DPPH free radicals were examined. The ability to quench the free radicals for the examined plant infusions increases in the following order: Asparagus racemosus (root) < Mitchella repens (herb) < Cnicus benedictus L. (herb) < Galega officinalis L. (herb) < Eupatorium cannabinum L. (herb). The analyzed infusions differ in the kinetics of the interactions with free radicals. The fastest interactions with free radicals characterize the infusions of Galega officinalis L. herb and Eupatorium cannabinum L. herb. The infusion of Mitchella repens herb interacts with free radicals in the slowest way. UVA radiation reduces the antioxidant interactions of all tested infusions, especially the infusion of Eupatorium cannabinum L. herb, which should be protected against UVA radiation during storage. The weakest decrease of free radical scavenging activity was observed for the infusion of the root of Asparagus racemosus exposed to UVA radiation. UVA radiation affected the speed of the free radical interactions of the infusions, depending on the type of plant materials. EPR spectroscopy is useful to examine the free radical scavenging activity of plant infusions, which is helpful to find effective antioxidants for applications in obstetrics and their optimal storage conditions.

Sign in / Sign up

Export Citation Format

Share Document