Multiple Sclerosis: Evaluation of Purine Nucleotide Metabolism in Central Nervous System in Association with Serum Levels of Selected Fat-Soluble Antioxidants

In the pathogenesis of demyelinating diseases including multiple sclerosis (MS) an important role is played by oxidative stress. Increased energy requirements during remyelination of axons and mitochondria failure is one of the causes of axonal degeneration and disability in MS. In this context, we analyzed to what extent the increase in purine catabolism is associated with selected blood lipophilic antioxidants and if there is any association with alterations in serum levels of coenzyme Q10. Blood serum and cerebrospinal fluid (CSF) samples from 42 patients with diagnosed MS and 34 noninflammatory neurologic patients (control group) were analyzed. Compared to control group, MS patients had significantly elevated values of all purine nucleotide metabolites, except adenosine. Serum lipophilic antioxidantsγ-tocopherol,β-carotene, and coenzyme Q10for the vast majority of MS patients were deficient or moved within the border of lower physiological values. Serum levels of TBARS, marker of lipid peroxidation, were increased by 81% in the MS patients. The results indicate that the deficit of lipophilic antioxidants in blood of MS patients may have a negative impact on bioenergetics of reparative remyelinating processes and promote neurodegeneration.

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PRPP and Purine Nucleotide Metabolism in Human Lymphoblasts with both PRPP Synthetase Superactivity and HGPRT Deficiency

Advances in Experimental Medicine and Biology - Purine and Pyrimidine Metabolism in Man VI ◽

10.1007/978-1-4684-5676-9_3 ◽

1989 ◽

pp. 13-20 ◽

Cited By ~ 2

Author(s):

Michael A. Becker ◽

Mitchel Kim ◽

Kishwar Husain

Keyword(s):

Nucleotide Metabolism ◽

Purine Nucleotide ◽

Purine Nucleotide Metabolism

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Investigation Into Effect Of Crude Mixture Of Abrus Precatorius Seed On Hypothalamopituitary Gonadal Axis And Development Of Antifertility In Male Rats

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v36i1.9234 ◽

1970 ◽

Vol 36 (1) ◽

pp. 103-109 ◽

Cited By ~ 1

Author(s):

S Talukder ◽

MA Hossain ◽

S Sarker ◽

MAH Khan

Keyword(s):

Negative Impact ◽

Sperm Count ◽

Sharp Decrease ◽

Serum Levels ◽

Serum Biochemistry ◽

Male Rats ◽

Male Rat ◽

Control Group ◽

Abrus Precatorius ◽

Crude Mixture

To evaluate the antifertility effect of crude mixture of A. precatorius seeds at the dose level of 50 mg/kg body weight in adult male rats, after oral administration to male rats for 40 days, the rats were sacrificed and hormonal profiles, serum biochemistry, sperm count and histological changes were recorded. A sharp decrease in the serum levels of testosterone (0.70 ± 0.17 ng/ml), FSH (0.70 ± 0.22 lU/L), and LH (0.87 ± 0.35 IU/L) was detected compared to control (FSH, LH and testosterone levels 0.93 ± 0.15 ng/ml, 0.76 ± 0.28 IU/L, 1.44 ± .011 IU/L, respectively). A significant reduction of epididymal sperm count (2.34 million/mL) was noted in treated rats as compared to control group (7.87 million/mL). Histology of testes showed marked atrophy of the testes, which was characterized by disruption of the seminiferous epithelium and atrophy of the Leydig cells. Crude mixture of A. precatorius seed has a negative impact on male reproductive functions. It might be suggested that crude mixture of A. precatorius seeds might have antifertility property for male rats. Keywords: Abrus precatorius; antifertility; male rat; testosterone. DOI: http://dx.doi.org/10.3329/bjar.v36i1.9234 BJAR 2011; 36(1): 103-109

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Clinical presentation and course of multiple sclerosis in patients with herpesvirus infection

Neurology neuropsychiatry Psychosomatics ◽

10.14412/2074-2711-2021-1s-21-26 ◽

2021 ◽

Vol 13 (1S) ◽

pp. 21-26

Author(s):

M. S. Gris ◽

N. S. Baranova ◽

N. N. Spirin ◽

D. S. Kasatkin ◽

D. V. Kiselev ◽

...

Keyword(s):

Multiple Sclerosis ◽

Clinical Presentation ◽

Serum Levels ◽

Main Group ◽

Control Group ◽

Herpesvirus Infection ◽

Varicella Zoster ◽

Barr Virus ◽

Ebv Infection ◽

Hsv 1

To date, the features of clinical presentation, course, and the effectiveness of therapy for multiple sclerosis (MS) in the presence of persistent herpesvirus infection (PHVI) remain poorly understood.Objective: to evaluate the features of clinical presentation and course of MS in patients with PHVI to optimize patient management.Patients and methods. We examined 122 patients with a clinically definite diagnosis of MS according to McDonald criteria (2010) (82 women and 40 men, age: 18–50 years, mean age – 37.74±11.04 years). MS duration at the time of examination was from 6 months to 20 years (mean – 8.53±7.47 years), mean Expanded Disability Status Scale (EDSS) score – 2.91±1.67. 86% of patients had relapsing-remitting MS; 14% – secondary progressive MS. 98 (80%) patients received disease modifying therapies (DMTs). All patients underwent a comprehensive clinical and neurological examination, magnetic resonance imaging (MRI). 30 healthy donors (20 women and 10 men, age: 19–62 years, mean age: 39.1±12.1 years) were included in the control group. Serum levels of type-specific IgM and IgG antibodies to herpes simplex virus (HSV) 1, 2, 6, Varicella zoster virus (VVZ), Epstein–Barr virus (EBV), cytomegalovirus (CMV) were detected, in some patients – blood and cerebrospinal fluid (CSF) polymerase chain reaction, serum and CSF oligoclonal IgG.Results and discussion. We identified two sub-groups of MS patients: with PHVI reactivation (main group, n=29) and without it (comparison group, n=93). There were a significantly higher VZV (72%) and EBV infection rate (100%) in MS patients compared to the control group (50% and 83%, respectively). Mixed herpesvirus infection prevailed over mono-infection in MS patients. In contrast to controls, the most common viral pattern in MS group was a combination of 4 herpes viruses: HSV 1, 2 + VZV + EBV + CMV (χ2=3.9; p<0.05). Patients in the main group had an unfavorable disease course: earlier MS onset, predominantly polysymptomatic onset, significantly higher relapse rate, faster disease progression, and higher EDSS and Functional Systems Scale (FSS) scores (p <0.05). MRI activity was also associated with EBV infection: new T1Gd+ and T2 foci were associated with an increase in VCA-IgM to EBV level. We also observed decreased DMTs effectiveness (χ2=4,6; p=0,033) and worse DMTs tolerability (χ2=5,3; p=0,022) in the main group.Conclusion. MS patients with PHVI reactivation, have a more unfavorable course of the demyelinating process and, therefore, a greater degree of disability, compared with age-adjusted patients without a viral infection and the same disease duration.

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Effect of COVID-19 Lockdown on Patients with Multiple Sclerosis: an Italian Survey

10.47363/jnrrr/2021(3)138 ◽

2021 ◽

pp. 1-7

Author(s):

Marta Altieri ◽

◽

Mariangela Fratino ◽

Flavia Pauri ◽

Antonella Conte ◽

...

Keyword(s):

Multiple Sclerosis ◽

Family Caregiving ◽

Social Life ◽

Negative Impact ◽

Self Report ◽

Psychological Status ◽

Control Group ◽

Clinical Effects ◽

Future Expectations ◽

The Impact

Background: Italy was one of the first affected countries by coronavirus disease 2019 (COVID-19) pandemic. Public health measures like quarantine or national lockdown were adopted, with negative psychological and clinical effects on patients with chronic diseases. To investigate the impact of lockdown on patients with multiple sclerosis (MS), we developed a 36 items self-report questionnaire. Methods: Questionnaires were emailed to 120 patients with MS and 100 with chronic migraine (CM) as a control group, matched for age, sex, and education. The questionnaire was divided into five domains, evaluating concerns about 1) MS and therapy during COVID-19 pandemic, 2) personal and family caregiving, 3) working activities, 4) general and disease-related emotions during the lockdown, 5) future expectations concerning health status, social life, and working activity. Results: patients with MS had higher scores than those with CM in domains 4 and 5, investigating respectively general and disease-related emotions and future expectations (p= 0.05 and 0.02 respectively). About half of the patients with MS expressed some concern about the need to continue their therapy during the pandemic compared to people with CM (p= 0.0002). Conclusions: Covid-19 pandemic had a more negative impact on psychological status of patients with MS compared with those with CM

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Some Aspects of Purine Nucleotide Metabolism in Lymphocytes of B-CLL

Tumori Journal ◽

10.1177/030089169107700204 ◽

1991 ◽

Vol 77 (2) ◽

pp. 112-117 ◽

Cited By ~ 3

Author(s):

Antonella Tabucchi ◽

Roberto Leoncini ◽

Roberto Pagani ◽

Maria Pizzichini ◽

Lucia Terzuoli ◽

...

Keyword(s):

Nucleotide Metabolism ◽

Purine Nucleotide ◽

Purine Nucleotide Metabolism

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Interleukin-10 and brain-derived neurotrophic factor responses to the Mat Pilates training in women with multiple sclerosis

Scientia Medica ◽

10.15448/1980-6108.2018.4.31668 ◽

2018 ◽

Vol 28 (4) ◽

pp. 31668

Author(s):

Elham Eftekhari ◽

Masoud Etemadifar

Keyword(s):

Multiple Sclerosis ◽

Neurotrophic Factor ◽

Training Group ◽

Serum Levels ◽

Brain Derived Neurotrophic Factor ◽

Interleukin 10 ◽

Analysis Of Covariance ◽

Control Group ◽

P Value ◽

Before And After

AIMS: To determine the effect of Mat Pilates on serum levels of interleukin-10 and brain-derived neurotrophic factor in women with multiple sclerosis.METHODS: Thirty women with multiple sclerosis with mild to moderate disability were recruited and randomly divided into equal Pilates training and Control groups. Patients in the training group accomplished a Pilates program three times a week for eight weeks. The Control group maintained their routine lifestyle. The serum level of interleukin-10 and brain-derived neurotrophic factor were measured before and after the protocol. The differences between groups were assessed by using analysis of covariance test to compare post-tests by considering covariate pre-tests (assuming a p-value <0.05 as significant).RESULTS: There were no significant changes in interleukin-10 (13.09±5.36 ng/ml in the Pilates training group compared to 13.21±4.76 ng/ml in the Control group, p= 0.81), whereas an increase in brain-derived neurotrophic factor was observed after eight-week Pilates training (11550.14±2619.60 ng/ml in the Pilates training group compared to 9664.35±3161.66 ng/ml in the Control group, p= 0.03).CONCLUSIONS: The results suggest that the intensity and duration of this protocol was not related to significant changes in interleukin-10, but was followed by an increase in brain-derived neurotrophic factor in these patients. Based on this finding, physical activity according to the individual’s ability is recommended for patients with multiple sclerosis, in parallel with drug therapy.

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