scholarly journals Tandem Mass Spectrometry Detection of Quorum Sensing Activity in Multidrug Resistant Clinical IsolateAcinetobacter baumannii

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Kok-Gan Chan ◽  
Huey Jia Cheng ◽  
Jian Woon Chen ◽  
Wai-Fong Yin ◽  
Yun Fong Ngeow
Keyword(s):  
Mass Spectrometry ◽  
Quorum Sensing ◽  
Tandem Mass Spectrometry ◽  
Homoserine Lactone ◽  
Multidrug Resistant ◽  
Side Chain ◽  
Tandem Mass ◽  
Mass Spectrometry Detection ◽  
Liquid Chromatography Tandem Mass ◽  
Chain Lengths

ManyProteobacteriacommunicate via production followed by response of quorum sensing molecules, namely,N-acyl homoserine lactones (AHLs). These molecules consist of a lactone moiety withN-acyl side chain with various chain lengths and degrees of saturation at C-3 position. AHL-dependent QS is often associated with regulation of diverse bacterial phenotypes including the expression of virulence factors. With the use of biosensor and high resolution liquid chromatography tandem mass spectrometry, the AHL production of clinical isolateA. baumannii4KT was studied. Production of short chain AHL, namely,N-hexanoyl-homoserine lactone (C6-HSL) andN-octanoyl-homoserine lactone (C8-HSL), was detected.

scholarly journals Identification of Quorum-Sensing Molecules of N-Acyl-Homoserine Lactone in Gluconacetobacter Strains by Liquid Chromatography-Tandem Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2694
Author(s):  
Ling-Pu Liu ◽  
Long-Hui Huang ◽  
Xiao-Tong Ding ◽  
Lin Yan ◽  
Shi-Ru Jia ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Quorum Sensing ◽  
Tandem Mass Spectrometry ◽  
Homoserine Lactone ◽  
Tandem Mass ◽  
Gram Negative ◽  
Sensing Systems ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Many Gram-negative bacteria can regulate gene expression in a cell density-dependent manner via quorum-sensing systems using N-acyl-homoserine lactones (AHLs), which are typical quorum-sensing signaling molecules, and thus modulate physiological characteristics. N-acyl-homoserine lactones are small chemical molecules produced at low concentrations by bacteria and are, therefore, difficult to detect. Here, a biosensor system method and liquid chromatography-tandem mass spectrometry were combined to detect and assay AHL production. As demonstrated by liquid chromatography-tandem mass spectrometry, Gluconacetobacter xylinus CGMCC No. 2955, a Gram-negative acetic acid-producing bacterium and a typical bacterial cellulose (BC) biosynthesis strain, produces six different AHLs, including N-acetyl-homoserine lactone, N-butanoyl-homoserine lactone, N-hexanoyl-homoserine lactone, N-3-oxo-decanoyl-homoserine lactone, N-dodecanoyl-homoserine lactone, and N-tetradecanoyl-homoserine lactone. Gluconacetobacter sp. strain SX-1, another Gram-negative acetic acid-producing bacterium, which can synthesize BC, produces seven different AHLs including N-acetyl-homoserine lactone, N-butanoyl-homoserine lactone, N-hexanoyl-homoserine lactone, N-3-oxo-octanoyl-homoserine lactone, N-decanoyl-homoserine lactone, N-dodecanoyl-homoserine lactone, and N-tetradecanoyl-homoserine lactone. These results lay the foundation for investigating the relationship between BC biosynthesis and quorum-sensing systems.

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