scholarly journals Nanotoxic Profiling of Novel Iron Oxide Nanoparticles Functionalized with Perchloric Acid and SiPEG as a Radiographic Contrast Medium

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Muhamad Idham Mohamed ◽  
Mohd Khairul Amran Mohammad ◽  
Hairil Rashmizal Abdul Razak ◽  
Khairunisak Abdul Razak ◽  
Wan Mazlina Md Saad
Keyword(s):  
Lipid Peroxidation ◽  
Iron Oxide ◽  
Contrast Medium ◽  
Iron Oxide Nanoparticles ◽  
Perchloric Acid ◽  
Metallic Nanoparticles ◽  
Oxide Nanoparticles ◽  
Radiographic Contrast Medium ◽  
Hematological Analysis ◽  
Radiographic Contrast

Emerging syntheses and findings of new metallic nanoparticles (MNPs) have become an important aspect in various fields including diagnostic imaging. To date, iodine has been utilized as a radiographic contrast medium. However, the raise concern of iodine threats on iodine-intolerance patient has led to search of new contrast media with lower toxic level. In this animal modeling study, 14 nm iron oxide nanoparticles (IONPs) with silane-polyethylene glycol (SiPEG) and perchloric acid have been assessed for toxicity level as compared to conventional iodine. The nanotoxicity of IONPs was evaluated in liver biochemistry, reactive oxygen species production (ROS), lipid peroxidation mechanism, and ultrastructural evaluation using transmission electron microscope (TEM). The hematological analysis and liver function test (LFT) revealed that most of the liver enzymes were significantly higher in iodine-administered group as compared to those in normal and IONPs groupsP<0.05. ROS production assay and lipid peroxidation indicator, malondialdehyde (MDA), also showed significant reductions in comparison with iodine groupP<0.05. TEM evaluation yielded the aberration of nucleus structure of iodine-administered group as compared to those in control and IONPs groups. This study has demonstrated the less toxic properties of IONPs and it may postulate that IONPs are safe to be applied as radiographic contrast medium.

Application of Iron Oxide Nanoparticles in Contemporary Experimental Physiology and Cell Biology Research

2018 ◽  
Vol 53 (1) ◽  
pp. 74-78 ◽  
Author(s):  
Dubravka Nikolovski ◽  
Marta Jeremic ◽  
Jovana Paunovic ◽  
Danijela Vucevic ◽  
Tatjana Radosavljevic ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cell Biology ◽  
Metallic Nanoparticles ◽  
Oxide Nanoparticles ◽  
Short Article ◽  
Recent Developments ◽  
The Impact ◽  
Biology Research

Abstract Recent findings have suggested that iron oxide nanoparticles (IONPs) have some exceptional chemical characteristics which make them useful in both experimental physiology and cell biology research. These nanoparticles might be applied as drug delivery systems for anti-cancer and other medications. Also, IONPs might be a valuable part of many novel bioassays in various fundamental medical fields. In recent years, several studies have indicated that IONPs may have certain cytotoxic and genotoxic potential in living systems. During in vitro conditions, IONPs might induce generation of reactive oxygen species and cause oxidative stress in some cell populations. The toxicity of IONPs is not yet fully understood, and additional research is needed to confirm if IONPs have detrimental effects on human health. This short article focuses on the recent developments and trends in the fields of nanomedicine and nanobiology regarding iron oxide nanomaterials and their application in fundamental medical disciplines such as experimental physiology. We discuss our previously published works on structural effects of IONPs and other metallic nanoparticles on cell nucleus in in vitro conditions. We also describe our recent findings regarding the impact of IONPs on certain biophysical properties of subcellular components.

Lipid peroxidation and its control in Anguilla anguilla hepatocytes under silica-coated iron oxide nanoparticles (with or without mercury) exposure

2015 ◽  
Vol 22 (13) ◽  
pp. 9617-9625 ◽  
Author(s):  
Koigoora Srikanth ◽  
Naser A. Anjum ◽  
Tito Trindade ◽  
Armando C. Duarte ◽  
Edurda Pereira ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Anguilla Anguilla ◽  
Mercury Exposure ◽  
Oxide Nanoparticles

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

Ferrozine Assay for Simple and Cheap Iron Analysis of Silica-Coated Iron Oxide Nanoparticles

2018 ◽  
Author(s):  
Hattie Ring ◽  
Zhe Gao ◽  
Nathan D. Klein ◽  
Michael Garwood ◽  
John C. Bischof ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Hydrofluoric Acid ◽  
Inductively Coupled Plasma ◽  
Silica Shell ◽  
Oxide Nanoparticles ◽  
Inductively Coupled ◽  
Digestion Process ◽  
Plasma Methods ◽  
Iron Analysis

The Ferrozinen assay is applied as an accurate and rapid method to quantify the iron content of iron oxide nanoparticles (IONPs) and can be used in biological matrices. The addition of ascorbic aqcid accelerates the digestion process and can penetrate an IONP core within a mesoporous and solid silica shell. This new digestion protocol avoids the need for hydrofluoric acid to digest the surrounding silica shell and provides and accessible alternative to inductively coupled plasma methods. With the updated digestion protocol, the quantitative range of the Ferrozine assay is 1 - 14 ppm. <br>

Ferrozine Assay for Simple and Cheap Iron Analysis of Silica-Coated Iron Oxide Nanoparticles

2018 ◽  
Author(s):  
Hattie Ring ◽  
Zhe Gao ◽  
Nathan D. Klein ◽  
Michael Garwood ◽  
John C. Bischof ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Hydrofluoric Acid ◽  
Inductively Coupled Plasma ◽  
Silica Shell ◽  
Oxide Nanoparticles ◽  
Inductively Coupled ◽  
Digestion Process ◽  
Plasma Methods ◽  
Iron Analysis

The Ferrozinen assay is applied as an accurate and rapid method to quantify the iron content of iron oxide nanoparticles (IONPs) and can be used in biological matrices. The addition of ascorbic aqcid accelerates the digestion process and can penetrate an IONP core within a mesoporous and solid silica shell. This new digestion protocol avoids the need for hydrofluoric acid to digest the surrounding silica shell and provides and accessible alternative to inductively coupled plasma methods. With the updated digestion protocol, the quantitative range of the Ferrozine assay is 1 - 14 ppm. <br>

EB PVD synthesis of iron oxide nanoparticles and their biological activity

2020 ◽  
Vol 2020 (3) ◽  
pp. 54-61
Author(s):  
S.E. Litvin ◽  
◽  
Yu.A. Kurapov ◽  
E.M. Vazhnichaya ◽  
Ya.A. Stel’makh ◽  
...  
Keyword(s):  
Biological Activity ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles

Iron Oxide Nanoparticles: An Insight into their Biomedical Applications

2015 ◽  
Vol 22 (15) ◽  
pp. 1808-1828 ◽  
Author(s):  
Diana Couto ◽  
Marisa Freitas ◽  
Felix Carvalho ◽  
Eduarda Fernandes
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Biomedical Applications ◽  
Oxide Nanoparticles ◽  
Insight Into
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