EB PVD synthesis of iron oxide nanoparticles and their biological activity

2020 ◽  
Vol 2020 (3) ◽  
pp. 54-61
Author(s):  
S.E. Litvin ◽  
◽  
Yu.A. Kurapov ◽  
E.M. Vazhnichaya ◽  
Ya.A. Stel’makh ◽  
...  
Keyword(s):  
Biological Activity ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals Selected features of morpho-functional reactions of eukaryotic microorganisms grown in the presence of maghemite iron oxide nanoparticles obtained by laser target evaporation

2018 ◽  
Vol 185 ◽  
pp. 10004 ◽  
Author(s):  
Elena V. Simonova ◽  
Tatyana P. Denisova ◽  
Roman T. Khandukhanov ◽  
Grigory Yu. Melnikov ◽  
Anatoly I. Medvedev ◽  
...  
Keyword(s):  
Biological Activity ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Solid Medium ◽  
Oxide Nanoparticles ◽  
Significant Excess ◽  
Laser Target ◽  
Eukaryotic Microorganisms ◽  
Fe Ions ◽  
Number Of Cells

Biological activity of iron introduced into nutrient medium as a suspension of iron oxide nanoparticles (MNPs) synthesized by the laser target evaporation was investigated. Exophiala nigrum (E. nigrum) eukaryotes were grown either in the presence or in absence of MNPs. De-aggregated suspensions of Fe2.75O4 MNPs were added in concentrations of 1 to 104 maximum permissive dose (MPD, being 0.3 mg/L of Fe ions in water). Cells were exposed for 24 to 96 hours periods and then plated onto a solid medium. The effect of MNPs was evaluated by the change in the number of cells during exposure and the number and morphology of the colonies. For 1-10 MPD yeast showed unaltered characteristics. For 100 or 1000 MPD for 72 hours of exposure and above the number of cells increases up to 30 times in comparison with the control. A pronounced stimulating effect was revealed at 104 MPD of iron. A significant excess of the number of cells was observed for the first day. At exposures of 72 and 96 hours the differences in the number of cells in comparison with the control were 11 and 30 times, respectively. A change in the colonies morphology was observed at 100 MPD concentration.

Effects of 2,3-dimercaptosuccinic acid modified Fe2O3 nanoparticles on microstructure and biological activity of cardiomyocytes

RSC Advances ◽  
2015 ◽  
Vol 5 (25) ◽  
pp. 19493-19501 ◽  
Author(s):  
Yongchao Mou ◽  
Jin Zhou ◽  
Fei Xiong ◽  
Hong Li ◽  
Hongyu Sun ◽  
...  
Keyword(s):  
Biological Activity ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Dimercaptosuccinic Acid ◽  
Oxide Nanoparticles ◽  
Fe2o3 Nanoparticles ◽  
Intracellular Ros

Iron oxide nanoparticles did not interfere with the microstructure, but decreased the intracellular ROS content of cardiomyocytes.

scholarly journals Physical synthesis of iron oxide nanoparticles and their biological activity in vivo

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Yurii A. Kurapov ◽  
Elena M. Vazhnichaya ◽  
Stanislav E. Litvin ◽  
Sergey M. Romanenko ◽  
Gennadii G. Didikin ◽  
...  
Keyword(s):  
Biological Activity ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Physical Synthesis

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

Ferrozine Assay for Simple and Cheap Iron Analysis of Silica-Coated Iron Oxide Nanoparticles

2018 ◽  
Author(s):  
Hattie Ring ◽  
Zhe Gao ◽  
Nathan D. Klein ◽  
Michael Garwood ◽  
John C. Bischof ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Hydrofluoric Acid ◽  
Inductively Coupled Plasma ◽  
Silica Shell ◽  
Oxide Nanoparticles ◽  
Inductively Coupled ◽  
Digestion Process ◽  
Plasma Methods ◽  
Iron Analysis

The Ferrozinen assay is applied as an accurate and rapid method to quantify the iron content of iron oxide nanoparticles (IONPs) and can be used in biological matrices. The addition of ascorbic aqcid accelerates the digestion process and can penetrate an IONP core within a mesoporous and solid silica shell. This new digestion protocol avoids the need for hydrofluoric acid to digest the surrounding silica shell and provides and accessible alternative to inductively coupled plasma methods. With the updated digestion protocol, the quantitative range of the Ferrozine assay is 1 - 14 ppm. <br>

Ferrozine Assay for Simple and Cheap Iron Analysis of Silica-Coated Iron Oxide Nanoparticles

2018 ◽  
Author(s):  
Hattie Ring ◽  
Zhe Gao ◽  
Nathan D. Klein ◽  
Michael Garwood ◽  
John C. Bischof ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Hydrofluoric Acid ◽  
Inductively Coupled Plasma ◽  
Silica Shell ◽  
Oxide Nanoparticles ◽  
Inductively Coupled ◽  
Digestion Process ◽  
Plasma Methods ◽  
Iron Analysis

The Ferrozinen assay is applied as an accurate and rapid method to quantify the iron content of iron oxide nanoparticles (IONPs) and can be used in biological matrices. The addition of ascorbic aqcid accelerates the digestion process and can penetrate an IONP core within a mesoporous and solid silica shell. This new digestion protocol avoids the need for hydrofluoric acid to digest the surrounding silica shell and provides and accessible alternative to inductively coupled plasma methods. With the updated digestion protocol, the quantitative range of the Ferrozine assay is 1 - 14 ppm. <br>

Iron Oxide Nanoparticles: An Insight into their Biomedical Applications

2015 ◽  
Vol 22 (15) ◽  
pp. 1808-1828 ◽  
Author(s):  
Diana Couto ◽  
Marisa Freitas ◽  
Felix Carvalho ◽  
Eduarda Fernandes
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Biomedical Applications ◽  
Oxide Nanoparticles ◽  
Insight Into
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