Frequency Distribution of Mannose Binding Lectin-2 and Vitamin D Receptor Gene Variants: Putative Markers for Tuberculosis

Genetic polymorphism in Mannose Binding Lectin-2 (MBL-2) and Vitamin D Receptor (VDR) is known to influence the susceptibility to tuberculosis. The objective of the present study was to evaluate the frequency distribution of the MBL-2 promoter and structural polymorphism (−550 H/L, −221 Y/X, and +4 P/Q; R52C, G54D, and G57F) and VDR polymorphism (FokI, BsmI, TaqI, and ApaI) in healthy individuals of Indian population and comparative analysis with the global population. In Indian population, the frequency of VDR mutant alleles “f” for FokI, “b” for BsmI, “t” for TaqI, and “a” for ApaI was 25%, 54%, 30%, and 61%, respectively. The allelic frequency of MBL-2 promoter polymorphism −550 H/L was H versus L: 32% versus 68%, −221 Y/X was Y versus X: 68% versus 32%, and +4 P/Q was P versus Q: 78% versus 22%. Mutant allelic frequencies of the MBL-2 exon 1 D, B, and C allele were 6%, 11%, and 3%, respectively. Comparative analysis with global populations showed a noteworthy difference for MBL-2 and VDR polymorphism frequency distribution, indicating the ethnic variability of Indians. The study signifies the differential distribution of susceptibility genes in Indian population, which can influence the understanding of the pathophysiology of tuberculosis in Indian population.

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Elevated levels of vitamin D and deficiency of mannose binding lectin in dengue hemorrhagic fever

Virology Journal ◽

10.1186/1743-422x-9-86 ◽

2012 ◽

Vol 9 (1) ◽

pp. 86 ◽

Cited By ~ 19

Author(s):

Kalichamy Alagarasu ◽

Rupali V Bachal ◽

Asha B Bhagat ◽

Paresh S Shah ◽

Cecilia Dayaraj

Keyword(s):

Vitamin D ◽

Dengue Hemorrhagic Fever ◽

Hemorrhagic Fever ◽

Mannose Binding Lectin ◽

Mannose Binding ◽

Binding Lectin

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The frequency distribution of vitamin D Receptor fok I gene polymorphism among Ugandan pulmonary TB patients

F1000Research ◽

10.12688/f1000research.9109.1 ◽

2016 ◽

Vol 5 ◽

pp. 1890 ◽

Cited By ~ 2

Author(s):

Ester L. Acen ◽

William Worodria ◽

Peter Mulamba ◽

Andrew Kambugu ◽

Joseph Erume

Keyword(s):

Vitamin D ◽

Vitamin D Deficiency ◽

Frequency Distribution ◽

Vitamin D Receptor ◽

Receptor Gene ◽

P Value ◽

Genotype Distribution ◽

Pulmonary Tb ◽

Significant Difference ◽

I Gene

Background: Mycobacterium tuberculosis (TB) is still a major problem globally and especially in Africa. Vitamin D deficiency has been linked to TB in the past and studies have found vitamin D deficiency to be common among Ugandan TB patients. The functional activity of vitamin D is dependent on the genotype of the vitamin D receptor (VDR) polymorphic genes. Recent findings have indicated that VDR polymorphisms may cause increased resistance or susceptibility to TB. The vitamin D ligand and its receptor play a pivotal role in innate immunity by eliciting antimicrobial activity, which is important in prevention of TB. The fok I vitamin D receptor gene has extensively been examined in TB patients but findings so far have been inconclusive. Objectives: This study sought to investigate the frequency distribution of the VDR fok I gene polymorphisms in pulmonary TB patients and controls. Methods: A pilot case control study of 41 newly diagnosed TB patients and 41 healthy workers was set up. Vitamin D receptor fok I gene was genotyped. Results: The frequency distribution of fok I genotype in Ugandan TB patients was 87.8% homozygous-dominant (FF), 7.3% (Ff) heterozygous and 4.8% (ff) homozygous recessive. For normal healthy subjects the frequencies were (FF) 92.6%, (Ff) 2.4% and (ff) 4.8%. No significant difference was observed in the FF and ff genotypes among TB patients and controls. The Ff heterozygous genotype distribution appeared more in TB patients than in controls. A significant difference was observed in the fok I genotype among gender p value 0.02. No significant difference was observed in ethnicity, p value 0.30. Conclusions: The heterozygous Ff fok I genotype may be associated with TB in the Ugandan population.

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