scholarly journals Preliminary Phytochemical Screening and Biological Activities ofBulbine abyssinicaUsed in the Folk Medicine in the Eastern Cape Province, South Africa

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Cromwell Mwiti Kibiti ◽  
Anthony Jide Afolayan
Keyword(s):  
Free Radical ◽  
Antimicrobial Agents ◽  
Protein Denaturation ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Free Radical Scavenging ◽  
Phytochemical Screening ◽  
Opportunistic Bacteria ◽  
Anti Inflammatory

Bulbine abyssinicaA. Rich. is used in traditional medicine to treat rheumatism, dysentery, bilharzia, cracked lips, back pain, infertility, diabetes mellitus, and gastrointestinal, vaginal, and bladder infections. Therefore, preliminary phytochemical screening, antioxidant, anti-inflammatory, and antibacterial properties of the whole plant (acetone and aqueous extracts) were determined using standard procedures. Thein vitroantioxidant model assays revealed that the plant possesses free radical scavenging potential varying with free radical species. The species showed significant protein denaturation inhibitory activity with good protection against erythrocyte membrane lysis indicating anti-inflammatory potential. The results also showed that the species was active against the growth of all the selected eight diabetic status opportunistic bacteria except one. Moreover, the species is characterized by appreciable amounts of total phenols, flavonoids, flavanols, proanthocyanidins, and alkaloids. Traces amounts of saponins and tannins were also observed. Amongst the identified phytochemicals present, empirical searches identified them being antioxidant, anti-inflammatory, and antimicrobial agents. The identification of these phytochemical constituents with their known pharmacological properties indicates that this plant is a good source of the free radical scavenging, anti-inflammatory, and antimicrobial agents. These findings also account for the multipharmacological use ofB. abyssinicain fork medicine.

scholarly journals Biological activities and phytochemical screening of Elaeodendron croceum (Thunb.) DC. Leaves and Stem barks Extracts

2017 ◽  
Vol 9 (4) ◽  
pp. 566 ◽  
Author(s):  
Samuel Wale Odeyemi ◽  
Anthony Jide Afolayan
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Protein Denaturation ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Diffusion Method ◽  
Aqueous Leaf Extract ◽  
Anti Inflammatory

<p><strong>Background:</strong> From reports <em>Elaeodendron croceum</em> has been used traditionally for spiritual cleansing, anti-obesity, anti-inflammatory and for the management of diabetes. The leaves and bark extracts were therefore analyzed for its biological activities.</p><p><strong>Materials and methods:</strong> The antibacterial activities of <em>E. croceum</em> leaves and stem barks were evaluated against both Gram positive and gram negative bacteria using agar well diffusion method. The analysis for the phytochemical contents, antioxidant and anti-inflammatory activities of the leaves and barks were determined using standard procedures.</p><p><strong>Results:</strong> The <em>S. aureus</em>, <em>S. flexneri</em>, <em>E. faecalis</em> and <em>S. typhimurium</em> were susceptible to the aqueous leaves and stem barks extracts of <em>Elaeodendron croceum</em> while <em>S. aureus </em>was susceptible to only the aqueous leaf extract. The antibacterial activity showed MIC ranging from 0.625 – 2.5 mg/ml. The composition of flavonoids (52.72 mg/g), proanthocyanidins (32.66 mg/g), alkaloids (65.44 mg/g) and saponins (68.21 mg/g) were significantly higher in the aqueous leaf extract while phenols (94.13 mg/g) content was higher in the aqueous bark extract. The free radical scavenging activity using ABTS, FRAP and DPPH showed that the extracts possess good free radical scavenging activities with different free radical species that is comparable with ascorbic acid and also showed significant inhibitory activity on protein denaturation as an indication of anti-inflammatory potential.</p><strong>Conclusion:</strong> These findings support the folkloric usage of <em>Elaeodendron croceum</em> and indicates that it can be used to manage diseases caused by the tested organisms. The presence of alkaloids, flavonoids, total phenols and proanthocyanidins, previously suggested to have antioxidant, anti-inflammatory and antibacterial activity indicate that <em>E. croceum</em> is a potential source of anti-inflammatory, free radical scavenging and antimicrobial agents.

scholarly journals In vitro screening of antioxidant, free radical scavenging and antimicrobial potential of Micrococca mercurialis whole plant extracts

2019 ◽  
Vol 10 (4) ◽  
pp. 3251-3262
Author(s):  
Thendral Hepsibha B ◽  
Gayathri Devi R ◽  
Lijitha L
Keyword(s):  
Free Radical ◽  
Plant Extracts ◽  
Skin Diseases ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Whole Plant ◽  
Anti Inflammatory ◽  
Scientific Validation

Micrococca mercurialis is widely used for the treatment of sores, skin diseases, cold, fever, rheumatic pain, and infections. The present study aimed to study the antioxidant, free radical scavenging and antibacterial potential different whole plant extracts (aqueous, ethanol, petroleum ether) of Micrococca mercurialis by in vitro methods. The amount of phenols, tannins, flavonoids and Vitamin C were estimated by conventional methods. Free radical scavenging potential was assessed by DPPH and FRAP assays. HRBC membrane-stabilizing study was done to determine its anti-inflammatory property by in vitro method. Activity against Staphylococcus aureus and Bacillus subtilis were determined in Micrococca mercurialis extracts. The phytoconstituents in the extracts of Micrococca mercurialis were explored by GC-MS analysis. The extracts of Micrococca mercurialis exhibited varied in vitro antioxidant, free radical scavenging, antibacterial and anti-inflammatory activities. The GC–MS results confirmed the presence of twenty-nine phytoconstituents totally responsible for the biological activities of Micrococca mercurialis. This pilot study has provided a scientific validation for the folkloric use of Micrococca mercurialis against many infections and diseases.

scholarly journals LCMS/MS analysis and evaluation of anti-inflammatory and antioxidant activities of the polyphenol fraction of Litsea quinqueflora (Dennst.) Suresh

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Sumin Mary Jose ◽  
M Anilkumar
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Protein Denaturation ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Aluminium Chloride ◽  
Alcoholic Extract ◽  
Ms Analysis ◽  
Anti Inflammatory

The main aim of the work was to scientifically prove the anti-inflammatory property of the polyphenol-rich fraction of hydro-alcoholic leaf extract of Litsea quinqueflora (Dennst.) Suresh by protein denaturation and free radical scavenging activity. The polyphenol-rich fraction of hydro-alcoholic leaf extract was obtained via acid-alkali hydrolysis, followed by fractionation with chloroform and ethyl acetate. HPTLC profiling of the finally obtained ethyl acetate fraction and consequent derivatisation with aluminium chloride revealed the presence of flavonoids in a more purified form. LCMS/MS analysis tentatively identified the presence of bioactive polyphenolic compounds such as gallocatechin, sinapic acid, pinocembrin, paeonol and umbelliferone in the separated fraction. The polyphenol-rich fraction of hydro-alcoholic extract of leaves showed anti-denaturing activity in heat-induced bovine serum albumin denaturation with an IC50 value of 23.59 µg/ml and was statistically significant at 0.1% level. The antioxidant property of the polyphenol-rich fraction determined by its free radical scavenging ability against DPPH and ABTS showed IC50 values 122.98 and 135.44 µg/ml respectively and was also statistically significant at 0.1% level. Hence, the traditional use of Litsea quinqueflora as an anti-inflammatory agent can be attributed to the presence of polyphenols.

scholarly journals In Vitro and in Vivo Evaluation Of Pharmacological Activites of Pouzolzia Sanguinea

2021 ◽  
Vol 29 (2) ◽  
pp. 31-42
Author(s):  
MQ Ahsan ◽  
MT Alam ◽  
MMU Chowdhury ◽  
MT Nasim ◽  
SMS Islam
Keyword(s):  
Body Weight ◽  
Free Radical ◽  
Protein Denaturation ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Standard Drug ◽  
Phytochemical Constituents ◽  
Anti Inflammatory

Pouzolzia sanguinea grows in tropical and sub-tropical regions of Bangladesh, used for a variety of purposes including pain, rheumatoid kidney diseases in traditional medicine. The crude ethanolic leaf extract of P. sanguinea with its different fractions (ethanol, n-hexane, and chloroform) was investigated for phytochemical constituents, in vitro antioxidant, anti-inflammatory effects, in-vivo analgesic and antipyretic activities. Preliminary phytochemical constituents were identified by chemical group test. P. sanguinea fractionated extracts contain alkaloids, glycosides, tannins, flavonoids, saponins, gums, and amides. Antioxidant activity test was performed by both qualitative (TLC and Rf value) and quantitative tests (inhibition of DPPH free radical scavenging). Extracts exhibited significant (p <0.001, p <0.0001) inhibition of DPPH free radical scavenging activity as compared to the standard drug ascorbic acid at similar doses. In vitro anti-inflammatory activity was determined by protein denaturation of egg albumin method. The percent inhibition of protein denaturation in the experiment of ethanol extract was found significantly higher (p <0.0001) compared with chloroform and n-hexane extracts. In addition, in vivo analgesic and antipyretic effects were determined in mice by acetic acid-induced writhing and yeast-induced pyrexia methods. The ethanol extracts of P. sanguinea exhibited inhibition of writhing reflex on mice by 71.58% at the dose of 500 mg/kg body weight which had greater analgesic activity than other n-hexane and chloroform extracts. In the anti-pyretic test, fractional extracts ethanol, chloroform, and n-hexane at a dose of 500 mg/kg body weight significantly (p <0.05) decreased pyrexia in mice up to 3 h as compared with the positive control paracetamol drug at a dose of 150 mg/kg body weight. In our in vitro and in vivo study models, it is evident that Pouzolzia sanguinea fractionated extracts showed significant antioxidant, anti-inflammatory, analgesic, and antipyretic activities. J. Bio-Sci. 29(2): 31-42, 2021 (December)

scholarly journals Comparative screening of in-vitro free radical scavenging, anti-inflammatory and anti-haemolytic activities from non-polar solvent extracts of Pterocarpus marsupium

2017 ◽  
Vol 9 (3) ◽  
pp. 399
Author(s):  
Aruna LH ◽  
Sanjeevkumar CB ◽  
Amarvani PK ◽  
Ramesh L Londonkar
Keyword(s):  
Free Radical ◽  
Petroleum Ether ◽  
Protein Denaturation ◽  
Petroleum Ether Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Pterocarpus Marsupium ◽  
Anti Inflammatory

<p><em>Pterocarpus marsupium</em> is widely used as ‘Rasayana’ in ayurvedic system for curing several medical ailments. In view of this, petroleum ether and chloroform extracts of <em>Pterocarpus marsupium</em> bark was scrutinized to unfold free radical scavenging by measuring their capability for scavenging DPPH radical, ABTS, phosphomolybdenum assay as well as reducing power capacity assessment and anti- inflammatory activity by inhibition of protein denaturation. Additionally, RBC’S were also used to explore the potentiality of <em>Pterocarpus marsupium </em>different extracts to shield RBC in oxidative stress condition. Chloroform extract showed an effective pharmacological activity in all assays when compared with petroleum ether extract and their respective standards. This reveals the use of <em>Pterocarpus marsupium </em>as a potent source of antioxidant, anti-inflammatory, anti-haemolytic agent and would provide an ample opportunity for further investigation. Isolation of active compounds from this plant responsible for producing such bioactivities is under process.</p>

scholarly journals In Vivo and In Vitro Evaluation of Preventive Activity of Inflammation and Free Radical Scavenging Potential of Plant Extracts from Oldenlandia corymbosa L.

2021 ◽  
Vol 11 (19) ◽  
pp. 9073
Author(s):  
Mahci Al Bashera ◽  
Ashik Mosaddik ◽  
Gaber El-Saber Batiha ◽  
Mohammed Alqarni ◽  
Md. Ashraful Islam ◽  
...  
Keyword(s):  
Free Radical ◽  
Protein Denaturation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Anti Inflammatory

Aims: The present study evaluates the anti-inflammatory and antioxidant activity of the crude dichloromethane (CDCME), ethyl acetate (CEAE), and methanol (CMeE) extracts from the plant Oldenlandia corymbosa L. Background:Oldenlandia species have been popular among the people of the Indian subcontinent to treat several types of internal and external inflammation for a long time. Plant decoctions have been used to battle inflammation in cases of tonsilitis, pneumonia and cholecystitis, among others. Objective: The present work designed to demonstrate the properties of the previously mentioned plant extracts to prevent inflammation both in vivo and in vitro. This work is the first investigation of such extracts from this species and their relationship with anti-inflammatory activity. Method: The anti-inflammatory properties of the Oldenlandia corymbosa L. extracts were evaluated in vitro with the Red Blood Cell (RBC) membrane stabilization method and the protein denaturation method and in vivo with the carrageenan-induced paw oedema method. Furthermore, the free radical scavenging activity of the extracts was carried out with the 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical oxidation, total antioxidant capacity and iron reduction assay. Result: Both in vivo and in vitro studies showed that CDCME had the most predominant effects with the value of 80.5% for RBC membrane stabilization, 60% for inhibition of protein denaturation at the concentration of 1000 µg/mL and 63.28% (after 3 h, * p < 0.05) for inhibition of paw oedema (300 mg/kg bwt) compared to carrageenan-induced mice. The free radical scavenging activity was studied by DPPH, total antioxidant and reducing activity assay. CDCME showed scavenging activity in all the methods and an IC50 value of 473.86 µg/mL for DPPH method. Conclusions: The findings of the study remarked that CDCME of the plant has strong anti-inflammatory and antioxidant effects that validate the traditional use of the plant to get remedy from pain. Other: The plants Oldenlandiacorymbosa Linn were provided by the Bangladesh Council of Scientific and Industrial Research Laboratory campus, Rajshahi, Bangladesh. Experiments on animals were conducted by ethical permission of Institute of Biological Sciences, University of Rajshahi, Bangladesh (license no: 225/320-IAMEBBC/IBSc).

scholarly journals Antioxidant, anti-inflammatory and anti-hyperglycemic activity of aqueous and methanolic extract of Houttuynia cordata: an in vitro and in vivo study

2021 ◽  
Vol 16 (3) ◽  
pp. 145-154
Author(s):  
Careen Liza Pakyntein ◽  
Donkupar Syiem ◽  
Daiahun Thabah ◽  
Shelareen Ediemi Sunn
Keyword(s):  
Free Radical ◽  
Side Effects ◽  
Methanolic Extract ◽  
Protein Denaturation ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Inflammatory Activity ◽  
Houttuynia Cordata ◽  
Anti Inflammatory

Available synthetic antioxidants such as butylated hydroxyl anisole, butylated hydroxyl toluene, propyl gallate, and ascorbic acid exhibit several side effects. To curb these side effects, more effective, less toxic, and cost-effective drugs are required. Therefore, this study aims to screen and evaluate the antioxidant as well as the anti-inflammatory and antidiabetic potential of Houttuynia cordata collected from Mairang village, West Khasi Hills, Meghalaya, India using several standard methods. The aqueous and methanolic extracts of H. cordata were evaluated by screening their ability to scavenge 1,1-diphenyl-2-dipicrylhydrozyl, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid), hydrogen peroxide, and nitric oxide. Total phenol and flavonoid content was measured by Folin-Ciocalteau and by AlCl3 colorimetric method respectively. The anti-inflammatory activity of the plant was determined using the protein denaturation method. Methanolic and aqueous extracts of Houttuynia cordata exhibit varying free radical scavenging and anti-inflammatory activity. Among the extracts used in the study, the methanolic extract of Houttuynia cordata elicited a higher activity than the aqueous extract. Houttuynia cordata also elicited a marked reduction in blood glucose level of normal and alloxan-induced diabetic mice. Flavonoids, which have been reported to possess anti-inflammatory, enzyme inhibition, antimicrobial, anticancer, antiallergy, and antioxidant properties may contribute towards the free radical scavenging and anti-inflammatory effect of Houttuynia cordata.

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