Study of Biofilm Formation and Antibiotic Resistant Profile in Multidrug Resistant and Extensive Drug Resistance Pseudomonas Aeruginosa Isolated from Burn Wound Infections in Southwest Iran
Abstract Background: Pseudomonas aeruginosa is an opportunistic pathogen that has remained on the ‘top 10’ common hospital ‘superbugs’ worldwide for more than a decade. Study of biofilm formation and antibiotic resistant profile in multidrug resistant and extensive drug resistance P. aeruginosa isolated from burn wound infections in southwest IranMethods and Results: This study, which was performed in 110 P. aeruginosa isolates culture-positive reports. Assessment of biofilm formation via microtiteplate and congo red agar. Overall, 110 clinical P. aeruginosa isolates were confirmed from wound burn infections. The maximum resistance rate among P. aeruginosa isolates to antibiotics tested was as follow Piperacillin, ceftazidime, and minimum resistance rate among P. aeruginosa isolates to antibiotics tested such as ticarcillin-clavulanic acid. The isolates were then evaluating the MICs by using the E-test. only 7 isolates were confirmed as colistin-resistant. Colistin reference MICs for the The prevalence of MDR P. aeruginosa was 38% and XDR- P. aeruginosa was 22% respectively. One of P. aeruginosa isolates were PDR. In microtiteplate assay,76% of the isolates have ability for biofilm, formation, 40% were categorized as strong biofilm-formers; 32% were moderate; 21% were weak biofilm formers and 43% could not form any detectable biofilm. Conclusion: in our study development of resistance by P. aeruginosa to many antimicrobial agents is a great challenge in controlling its infections. Therefore, the transmission of these isolates to patients leads to higher resistance. Therefore, the necessary hygiene measurements should be taken for the prevention of transferring the P. aeruginosa isolates to hospitalized patients.