scholarly journals Influence of Type 2 Diabetes on Prevalence of Key Periodontal Pathogens, Salivary Matrix Metalloproteinases, and Bone Remodeling Markers in Sudanese Adults with and without Chronic Periodontitis

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Hasaan Gassim Mohamed ◽  
Shaza Bushra Idris ◽  
Manal Mustafa ◽  
Mutaz Faisal Ahmed ◽  
Anne Nordrehaug Åstrøm ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Polymerase Chain Reaction ◽  
Detection Limit ◽  
Chronic Periodontitis ◽  
Periodontal Pathogens ◽  
Chain Reaction ◽  
Pocket Depth ◽  
Bone Remodeling Markers ◽  
Polymerase Chain

This study compared the influence of type 2 diabetes on the occurrence of six periodontal pathogens in plaque samples of patients with and without chronic periodontitis. Levels of salivary MMP-8, MMP-9, RANKL, and OPG were also investigated. The study enrolled 31 patients with type 2 diabetes and chronic periodontitis (DM + CP), 29 with chronic periodontitis (CP), and 20 with type 2 diabetes (DM). Questionnaire-guided interviews were conducted and plaque index, bleeding on probing, and pocket depth were recorded. Polymerase chain reaction (PCR) was utilized to determine the prevalence of the bacteria. The levels of salivary molecules were determined by enzyme immunosorbent assay (ELISA). The CP group had the highest prevalence ofP. gingivalis(81.5%), followed by the DM + CP (59.3%) and DM (55.0%) groups (P>0.05). Similar trends were observed forP. intermediaandT. denticola. The prevalence ofT. forsythiawas 100% in both periodontitis groups compared to 90% in the DM group. There were no significant differences between the groups regarding the concentrations of MMP-8, MMP-9, or OPG. RANKL concentrations were below the detection limit. Our data show that type 2 diabetes has no significant influence on the prevalence of the investigated periodontal pathogens, or the levels of salivary MMP-8, MMP-9, and OPG.

Quantification of five putative periodontal pathogens in female patients with and without chronic periodontitis by real-time polymerase chain reaction

Anaerobe ◽  
2010 ◽  
Vol 16 (3) ◽  
pp. 234-239 ◽  
Author(s):  
Renato R.R. Braga ◽  
Maria Auxiliadora R. Carvalho ◽  
Oscar Bruña-Romero ◽  
Rodrigo E. Teixeira ◽  
José Eustáquio Costa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chronic Periodontitis ◽  
Periodontal Pathogens ◽  
Chain Reaction ◽  
Female Patients ◽  
Polymerase Chain

Detection and Quantification of Periodontal Pathogens in Smokers and Never-Smokers With Chronic Periodontitis by Real-Time Polymerase Chain Reaction

2014 ◽  
Vol 85 (10) ◽  
pp. 1450-1457 ◽  
Author(s):  
Mariana R. Guglielmetti ◽  
Ecinele F. Rosa ◽  
Daniele S. Lourenção ◽  
Gislene Inoue ◽  
Elaine F. Gomes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chronic Periodontitis ◽  
Periodontal Pathogens ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Never Smokers ◽  
Detection And Quantification

scholarly journals Association of polymorphisms of genes TCF7L2, FABP2, KCNQ1, ADIPOQ with the prognosis of the development of type 2 diabetes mellitus

2020 ◽  
Vol 92 (10) ◽  
pp. 40-47
Author(s):  
E. S. Mel’nikova ◽  
O. D. Rymar ◽  
A. A. Ivanova ◽  
S. V. Mustafina ◽  
M. Ju. Shapkina ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Polymerase Chain Reaction ◽  
Type 2 Diabetes Mellitus ◽  
Control Group ◽  
Chain Reaction ◽  
Men And Women ◽  
Polymerase Chain ◽  
Diabetes Mellitus 2

Aim.To study the possibility of using polymorphisms of genesTCF7L2,FABP2,KCNQ1,ADIPOQas markers for predicting the development of type 2 diabetes mellitus (T2D) in the population of Novosibirsk. Materials and methods.On the basis of prospective observation of a representative population sample of residents of Novosibirsk (HAPIEE), 2 groups were formed according to the case-control principle (case people who had diabetes mellitus 2 over 10 years of observation, and control people who did not developed disorders of carbohydrate metabolism). T2D group (n=443, mean age 56.26.7 years, men 29.6%, women 70.4%), control group (n=532, mean age 56.17.1 years, men 32.7%, women 67.3%). DNA was isolated by phenol-chloroform extraction. Genotyping was performed by the method of polymerase chain reaction with subsequent analysis of restriction fragment length polymorphism, polymerase chain reaction in real time. Statistical processing was carried out using the SPSS 16.0 software package. Results and discussion.No significant effect of rs1799883 of theFABP2gene, rs2237892 of theKCNQ1gene, and rs6773957 of theADIPOQgene on the risk of developing T2D was found. Genotypes TT and TC rs7903146 of theTCF7L2gene are genotypes for the risk of developing T2D (relative risk RR 3.90, 95% confidence interval CI 2.316.61,p0.001; RR 1.86, 95% CI 1.422.43,p0.001, respectively). The CC genotype rs7903146 of theTCF7L2gene is associated with a protective effect against T2D (RR 0.37, 95% CI 0.290.49,p0.001). When theTCF7L2gene is included in the model for assessing the risk of developing T2D rs7903146, it retains its significance in both men and women. Conclusion.The rs7903146 polymorphism of theTCF7L2gene confirmed its association with the prognosis of the development of T2D, which indicates the possibility of considering it as a candidate for inclusion in a diabetes risk meter. Variants of risk meters have been developed to assess the prognosis of the development of diabetes mellitus 2 in men and women aged 4569 years during 10 years of follow-up. The association with the prognosis of the development of T2D polymorphisms rs1799883 of theFABP2gene, rs2237892 of theKCNQ1gene and rs6773957 of theADIPOQgene was not found.

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