Activity of Proteolytic Enzymes and Level of Cystatin C in the Peripartum Period

Objectives. The aim of the study was to evaluate the activity of cathepsin B, collagenases, trypsin, and plasmin and concentration of cystatin C in serum of healthy pregnant women in peripartum period.Study Design. The study group included 45 women in uncomplicated pregnancies. Blood samples were collected in four time points. Enzyme activity was measured by spectrofluorometric method. The level of cystatin C was measured using immunonephelometric method.Results. Mean activity of cathepsin B and the level of serum cystatin C were significantly higher in the study group. Collagenase activity was significantly lower in the study group than the control group. No differences in collagenase, plasmin, and trypsin activity on each day of the peripartum period were found.Conclusion. High activity of cathepsin B and increased level of cystatin C are typical for women in late pregnancy. Those levels significantly decrease after delivery which can be associated with potential role of those markers in placental separation. The insignificant changes of cystatin C level in the peripartum period seem to exclude the possibility of using cystatin C as a marker for renal insufficiency in the peripartum period but additional research is necessary to investigate the matter further.

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Assessment of Subclinical Renal Glomerular and Tubular Dysfunction in Children with Beta Thalassemia Major

Children ◽

10.3390/children8020100 ◽

2021 ◽

Vol 8 (2) ◽

pp. 100

Author(s):

Asmaa A. Mahmoud ◽

Doaa M. Elian ◽

Nahla MS. Abd El Hady ◽

Heba M. Abdallah ◽

Shimaa Abdelsattar ◽

...

Keyword(s):

Cystatin C ◽

Kidney Injury ◽

Thalassemia Major ◽

Beta Thalassemia ◽

Good Survival ◽

Control Group ◽

Healthy Children ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Beta Thalassemia Major

Background: A good survival rate among patients with beta thalassemia major (beta-TM) has led to the appearance of an unrecognized renal disease. Therefore, we aimed to assess the role of serum cystatin-C as a promising marker for the detection of renal glomerular dysfunction and N-acetyl beta-D-glucosaminidase (NAG) and kidney injury molecule 1 (KIM-1) as potential markers for the detection of renal tubular injury in beta-TM children. Methods: This case-control study was implemented on 100 beta-TM children receiving regular blood transfusions and undergoing iron chelation therapy and 100 healthy children as a control group. Detailed histories of complete physical and clinical examinations were recorded. All subjected children underwent blood and urinary investigations. Results: There was a significant increase in serum cystatin-C (p < 0.001) and a significant decrease in eGFR in patients with beta-TM compared with controls (p = 0.01). There was a significant increase in urinary NAG, KIM-1, UNAG/Cr, and UKIM-1/Cr (p < 0.001) among thalassemic children, with a significant positive correlation between serum cystatin-C, NAG and KIM-1 as regards serum ferritin, creatinine, and urea among thalassemic patients. A negative correlation between serum cystatin-C and urinary markers with eGFR was noted. Conclusion: Serum cystatin-C is a good marker for detection of glomerular dysfunction. NAG and KIM-1 may have a predictive role in the detection of kidney injury in beta-TM children.

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The role of serum cystatin C in estimation of renal function in survivors of critical illness

Journal of Critical Care ◽

10.1016/j.jcrc.2020.07.005 ◽

2020 ◽

Vol 59 ◽

pp. 201-206

Author(s):

Jirarat Eiamcharoenying ◽

Win Kulvichit ◽

Nuttha Lumlertgul ◽

Tawatchai Chaiwatanarat ◽

Sadudee Peerapornratana ◽

...

Keyword(s):

Renal Function ◽

Critical Illness ◽

Cystatin C ◽

Serum Cystatin C ◽

Serum Cystatin

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Cystatin C-Based Evaluation of Kidney Function of HIV-Infected Children in Benin City, Southern Nigeria

International Journal of Nephrology ◽

10.1155/2012/861296 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Moses Temidayo Abiodun ◽

Nosakhare J. Iduoriyekemwen ◽

Phillip O. Abiodun

Keyword(s):

Kidney Disease ◽

Hiv Infection ◽

Kidney Function ◽

Cystatin C ◽

Mean Value ◽

Control Group ◽

Healthy Children ◽

Serum Cystatin C ◽

Serum Cystatin ◽

The Mean

Background. Human immunodeficiency virus (HIV) is now a confirmed risk factor for kidney disease with an increased burden in persons of African descent.Method. We measured the serum cystatin C levels of 205 ART-naive, HIV-infected children by an ELISA technique and compared them with the levels of apparently healthy children.Result. The mean ± SD serum cystatin C level of children with HIV infection was 1.01 ± 0.44 mg/L, significantly higher than the mean value in the control group, that is, 0.72 ± 0.20 mg/L (P=0.000). The mean ± SD cystatin C-based estimated GFR of children with HIV infection was 102.7 ± 31.0 mL/min/1.73 m2, significantly lower than 126.9 ± 28.5 mL/min/1.73 m2in the control group, (P=0.014). A significantly higher proportion of HIV-infected children compared to controls had eGFR < 90 mL/min/1.73 m2(21.5% versus 5.4%;P=0.00). The prevalence of chronic kidney disease (CKD) among the HIV-infected children was 10.7%. The cystatin C-based eGFR of the HIV-infected children ≥5 years old correlated positively with their CD4 count (r=0.23; P=0.022).Conclusion. There is a high prevalence of CKD among HIV-infected children, requiring regular monitoring of their kidney function using a cystatin C-based method.

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PENGARUH PEMBERIAN KONTRAS MEDIA NON IONIK LOW OSMOLAR INTRA VENA TERHADAP KADAR CYSTATIN–C SERUM PADA ORYCTALAGUS CUNICULUS

Indonesian Journal of Urology ◽

10.32421/juri.v15i2.354 ◽

1970 ◽

Vol 15 (2) ◽

Author(s):

Ariyo Sakso Bintoro ◽

Soetojo Soetojo ◽

Doddy M Soebadi

Keyword(s):

Cystatin C ◽

Sandwich Elisa ◽

Intervention Group ◽

Serum Levels ◽

Control Group ◽

Test Results ◽

Elisa Method ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Ionic Contrast

Objective: To measure effects of low osmolar non ionic contrast media on cystatin–C serum levels on different days after administration. Material and Method: Twenty-two oryctalagus cuniculus were divided into two groups of 11 subjects, each subject receiving intravenous injection of low osmolar non ionic contrast or a placebo solution (NaCl 0,9%). Cystatin-C serum levels of each subject were measured before injection, on days 1, 3, 7, and 10. Cystatin–C serum levels were determined with a sandwich ELISA method. Statistical analysis was performed with t–test. Results: Mean cystatin–C serum levels before injection was 0,00337 ± 0,00101 mg/L. Means from days 1, 3, 7, and 10 after injections were 0,00498 ± 0,00153 mg/L; 0,00565 ± 0,00247 mg/L; 0,00468 ± 0,00157, and 0,00339 ± 0,00188 mg/L respectively. Conclusion: Increase in serum cystatin–C levels on days 1, 3, and 7 was significant. On the 10th day no significant escalation was observed. Compared to the control group, there were significant differences in serum cystatin–C increase on days 1 and 3 in the intervention group, but no significant differences 7 and 10 days after injection.

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The role of serum cystatin C as an early predictor for the diagnosis of chronic kidney disease in Mosul City

Iraqi Journal of Pharmacy ◽

10.33899/iphr.2019.161197 ◽

2019 ◽

Vol 14 (1) ◽

pp. 76-78

Author(s):

Mohammed Khalid J. Al-Nori ◽

Akram J. Ahmed

Keyword(s):

Chronic Kidney Disease ◽

Kidney Disease ◽

Cystatin C ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Early Predictor

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Role of serum cystatin-C and beta-2 microglobulin as early markers of renal dysfunction in children with beta thalassemia major

International Journal of Nephrology and Renovascular Disease ◽

10.2147/ijnrd.s142824 ◽

2017 ◽

Vol Volume 10 ◽

pp. 261-268 ◽

Cited By ~ 2

Author(s):

Ola Galal Behairy ◽

Eman Abd Almonaem ◽

Neveen Abed ◽

Omima Abdel Haiea ◽

Rasha Zakaria ◽

...

Keyword(s):

Renal Dysfunction ◽

Cystatin C ◽

Thalassemia Major ◽

Beta Thalassemia ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Early Markers ◽

Beta 2 ◽

Beta 2 Microglobulin

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The Predictive Role of Serum Cystatin C Levels in Polycystic Ovary Syndrome in Adolescents

Journal of Pediatric and Adolescent Gynecology ◽

10.1016/j.jpag.2015.12.005 ◽

2016 ◽

Vol 29 (4) ◽

pp. 353-356 ◽

Cited By ~ 2

Author(s):

Mehmet Çınar ◽

Rıfat Taner Aksoy ◽

Ali İrfan Güzel ◽

Aytekin Tokmak ◽

Tuğba Çandar ◽

...

Keyword(s):

Polycystic Ovary Syndrome ◽

Cystatin C ◽

Polycystic Ovary ◽

Serum Cystatin C ◽

Ovary Syndrome ◽

Serum Cystatin ◽

Predictive Role

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Serum Cystatin C in Estimating Glomerular Filtration Rate

Journal of Medical Biochemistry ◽

10.2478/v10011-007-0042-4 ◽

2008 ◽

Vol 27 (1) ◽

pp. 46-51 ◽

Cited By ~ 1

Author(s):

Velibor Čabarkapa ◽

Zoran Stošić ◽

Mirjana Đerić ◽

Ljiljana Vučurević-Ristić ◽

Radmila Žeravica ◽

...

Keyword(s):

Glomerular Filtration Rate ◽

Renal Insufficiency ◽

Glomerular Filtration ◽

Cystatin C ◽

Chronic Renal Insufficiency ◽

Filtration Rate ◽

Control Group ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Subgroup Ii

Serum Cystatin C in Estimating Glomerular Filtration RateUsing serum cystatin C in estimating glomerular filtration rate (GFR) has in recent times been recommended. A number of simple formulas for calculating GFR have been derived specifically from serum cystatin C concentrations. The purpose of this study was to assess the significance of cystatin C and of the two most frequently applied of these formulas in estimating glomerular filtration rate compared to serum creatinine and its derived formulas for estimating glomerular filtration rate from creatinine concentrations. The study included 74 patients: 59 were in various stages of chronic renal insufficiency (divided into two subgroups: I with GFR ≥ 60 mL/min/1.73m2and II with GFR<60 mL/min/1.73m2) and 15 on hemodialysis. A control group of 30 healthy participants was also included in the study. Serum values of cystatin C ranged from: 0.86 ± 0.16 mg/L in subgroup I, and 1.77 ± 0.79 mg/L in subgroup II, to 6.9 ± 1.83 mg/L in patients on hemodialysis. The correlation between the two formulas derived from cystatin C and the clearance of creatinine, as well as the Cockcroft and Gault's formula, was significant, while one of the formulas derived from cystatin C did not show a significant correlation with MDRD. It was concluded that serum cystatin C is a significant marker in estimating glomerular filtration rate, especially in the advanced stages of chronic renal insufficiency.

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