scholarly journals Antinociceptive Effect ofTephrosia sinapouExtract in the Acetic Acid, Phenyl-p-benzoquinone, Formalin, and Complete Freund’s Adjuvant Models of Overt Pain-Like Behavior in Mice

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Renata M. Martinez ◽  
Ana C. Zarpelon ◽  
Talita P. Domiciano ◽  
Sandra R. Georgetti ◽  
Marcela M. Baracat ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antinociceptive Effect ◽  
Complete Freund’S Adjuvant ◽  
Dependent Manner ◽  
Routes Of Administration ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Tephrosia toxicaria, which is currently known asTephrosia sinapou(Buc’hoz) A. Chev. (Fabaceae), is a source of compounds such as flavonoids.T. sinapouhas been used in Amazonian countries traditional medicine to alleviate pain and inflammation. The purpose of this study was to evaluate the analgesic effects ofT. sinapouethyl acetate extract in overt pain-like behavior models in mice by using writhing response and flinching/licking tests. We demonstrated in this study thatT. sinapouextract inhibited, in a dose (1–100 mg/kg) dependent manner, acetic acid- and phenyl-p-benzoquinone- (PBQ-) induced writhing response. Furthermore, it was active via intraperitoneal, subcutaneous, and peroral routes of administration.T. sinapouextract also inhibited formalin- and complete Freund’s adjuvant- (CFA-) induced flinching/licking at 100 mg/kg dose. In conclusion, these findings demonstrate thatT. sinapouethyl acetate extract reduces inflammatory pain in the acetic acid, PBQ, formalin, and CFA models of overt pain-like behavior. Therefore, the potential of analgesic activity ofT. sinapouindicates that it deserves further investigation.

scholarly journals Anti-Arthritic Potential of Ethyl Acetate Extract of Stereospermum colais in Animal Model

2021 ◽  
Vol 10 (14) ◽  
pp. 991-998
Author(s):  
Prema Suseela ◽  
Chitra Krishnan
Keyword(s):  
Nitric Oxide ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Complete Freund’S Adjuvant ◽  
Tnf Alpha ◽  
Acid Activation ◽  
Garcinia Indica ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

BACKGROUND Stereospermum colais also known as yellow snake tree is widely utilised to alleviate rheumatic pain and inflammation in the conventional medicinal system. Lapachol has been reported to be anti-inflammatory by inhibiting the production of nitric oxide (NO) and tumour necrosis factor (TNF)-alpha by means of modulating the metabolism of arachidonic acid, activation of NF-aB, suppression of nitric oxide synthase (iNOS) expression and expression of cyclooxygenase-2 (COX-2). It has also been analysed for anticancer and antioxidant activity, renal disorders, endometriosis and cardiac dysfunction. The present study investigates the anti– arthritic activity of the ethyl acetate extracts of Stereospermum colais. METHODS The fruit rind of Garcinia indica was used to prepare extract and was quantified using liquid chromatography–mass spectrometry (LC-MS) / MS. Ethyl acetate extract showed increased content of the phytochemical constituent necessary for the treatment of arthritic pain. So, the ethyl acetate extract of bark of S. colais was evaluated for anti-arthritic activity by complete Freund's adjuvant (CFA). Arthritis index, body weight changes, and the biochemical analysis parameters were measured. Histopathological evaluation along with TNF-alpha and interleukin (IL)-6 assays were also studied. RESULTS The ethyl acetate extract showed significant reduction in arthritis index (P < 0.01), paw swelling (P < 0.01) and arthritic score (P < 0.01), thereby demonstrating antiinflammatory potential. A good improvement in the biochemical parameters in extract treated animals indicates good protection against the inflammation. CONCLUSIONS The results show that Stereospermum colais can be used as a potential anti arthritic drug. KEY WORDS Rheumatoid Arthritis, Complete Freund’s Adjuvant, Stereospermum colais, Ethyl Acetate, Anti-Arthritis, Meloxicam

Moxibustion at ST36 Alleviates Pain in Complete Freund's Adjuvant-Induced Arthritic Rats

2006 ◽  
Vol 34 (01) ◽  
pp. 57-67 ◽  
Author(s):  
Jae-Hyo Kim ◽  
Hee-Kee Kim ◽  
Yong-Il Park ◽  
In-Churl Sohn ◽  
Dong-Ok Choi ◽  
...  
Keyword(s):  
Protein Expression ◽  
Rat Model ◽  
Antinociceptive Effect ◽  
Complete Freund’S Adjuvant ◽  
No Production ◽  
Western Blots ◽  
Antinociceptive Effects ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

This study was to investigate the antinociceptive effects of moxibustion in a complete Freund's adjuvant (CFA)-induced arthritic rat model, and the effects of moxibustion on immunohistochemical changes at the spinal cord level. Moxibustion was applied to the ipsilateral (right) Zusanli (ST36) acupoint to the lesion side for 9 days to CFA-induced arthritic rats. The stepping force was measured as a behavioral test, c-Fos immunohistochemistry, NO production and nNOS Western blots were examined to evaluate antinociceptive effects. Moxibustion at ST36 significantly improved the stepping force in the affected hind limb in CFA-induced arthritis. Moreover, moxibustion at ST36 suppressed the production of NO and the protein expression of c-Fos and nNOS induced by arthritis. These results suggest that moxibustion at ST36 has a potent antinociceptive effect in an arthritic rat model, and modulates neuronal excitability and endogenous NO production by suppressing c-Fos and nNOS protein expression.

scholarly journals Inhibitory Effect of Paquinimod on a Murine Model of Neutrophilic Asthma Induced by Ovalbumin with Complete Freund’s Adjuvant

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Jong-Uk Lee ◽  
Jong Sook Park ◽  
Ji Ae Jun ◽  
Min Kyung Kim ◽  
Hun Soo Chang ◽  
...  
Keyword(s):  
Murine Model ◽  
Complete Freund’S Adjuvant ◽  
Dependent Manner ◽  
Neutrophilic Inflammation ◽  
Caspase 1 ◽  
Tnf Α ◽  
Ifn Γ ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Background. Quinoline-3-carboxamides have been used to treat autoimmune/inflammatory diseases in humans because they inhibit the functions of S100 calcium-binding protein A9 (S100A9), which participates in the development of neutrophilic inflammation in asthmatics and in an animal model of neutrophilic asthma. However, the therapeutic effects of these chemicals have not been evaluated in asthma. The purpose of this study was to evaluate the effect of paquinimod, one of the quinoline-3-carboxamides, on a murine model of neutrophilic asthma. Methods. Paquinimod was orally administered to 6-week-old C57BL/6 mice sensitized and challenged with ovalbumin (OVA)/complete Freund’s adjuvant (CFA) and OVA. Lung inflammation and remodeling were evaluated using bronchoalveolar lavage (BAL) and histologic findings including goblet cell count. S100A9, caspase-1, IL-1β, MPO, IL-17, IFN-γ, and TNF-α were measured in lung lysates using western blotting. Results. Paquinimod restored the enhancement of airway resistance and the increases in numbers of neutrophils and macrophages of BAL fluids and those of goblet cells in OVA/CFA mice toward the levels of sham-treated mice in a dose-dependent manner (0.1, 1, 10, and 25 mg/kg/day, p.o.). Concomitantly, p20 activated caspase-1, IL-1β, IL-17, TNF-α, and IFN-γ levels were markedly attenuated. Conclusion. These data indicate that paquinimod effectively inhibits neutrophilic inflammation and remodeling in the murine model of neutrophilic asthma, possibly via downregulation of IL-17, IFN-γ, and IL-1β.

scholarly journals Electroacupuncture Confers Antinociceptive Effects via Inhibition of Glutamate Transporter Downregulation in Complete Freund's Adjuvant-Injected Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Ha-Neui Kim ◽  
Yu-Ri Kim ◽  
Ji-Yeon Jang ◽  
Hwa-Kyoung Shin ◽  
Byung-Tae Choi
Keyword(s):  
Thermal Sensitivity ◽  
Antinociceptive Effect ◽  
Glutamate Transporter ◽  
Complete Freund’S Adjuvant ◽  
Double Labeling ◽  
Mrna Content ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Glutamate Transporter 1

When we evaluated changes of glial fibrillary acidic protein (GFAP) and two glutamate transporter (GTs) by immunohistochemistry, expression of GFAP showed a significant increase in complete Freund's adjuvant (CFA)-injected rats; however, this expression was strongly inhibited by electroacupuncture (EA) stimulation. Robust downregulation of glutamate-aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1) was observed in CFA-injected rats; however, EA stimulation resulted in recovery of this expression. Double-labeling staining showed co-localization of a large proportion of GLAST or GLT-1 with GFAP. Using Western blot, we confirmed protein expression of two GTs, but no differences in the mRNA content of these GTs were observed. Because EA treatment resulted in strong inhibition of CFA-induced proteasome activities, we examined the question of whether thermal sensitivities and GTs expression could be regulated by proteasome inhibitor MG132. CFA-injected rats co-treated with EA and MG132 showed a significantly longer thermal sensitivity, compared with CFA-injected rats with or without MG132. Both EA and MG132 blocked CFA-induced GLAST and GLT-1 downregulation within the spinal cord. These results provide evidence for involvement of GLAST and GLT-1 in response to activation of spinal astrocytes in an EA antinociceptive effect. Antinociceptive effect of EA may be induced via proteasome-mediated regulation of spinal GTs.

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