scholarly journals Ethyl Acetate Extracts of Semen Impatientis Inhibit Proliferation and Induce Apoptosis of Human Prostate Cancer Cell Lines through AKT/ERK Pathways

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Tao Wang ◽  
Yang Cai ◽  
Wen Song ◽  
Ruibao Chen ◽  
Dunmei Hu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Western Blot ◽  
Ethyl Acetate ◽  
Caspase 3 ◽  
Inhibitory Effect ◽  
G1 Phase ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Impatiens Balsamina ◽  
Ethyl Acetate Extracts

Objective. To investigate the inhibitory effect of ethyl acetate extracts of Impatiens balsamina L. on prostate cancer cells. Methods. Impatiens balsamina L. was extracted to get water, ethanol, oil ether, ethyl acetate, and butanol extracts. CCK-8 assay was used to detect the inhibitory effect. Apoptosis rates and cell cycle distribution were detected by flow cytometry. Transwell assay was performed to test the ability of migration. The expressions of Bcl-2, Bax, cleaved-caspase-3, p-ERK, ERK, p-AKT, AKT, cyclin D1, cyclin E, and MMP2 were detected by Western blot. Results. Ethyl acetate extracts had the strongest inhibitory effect. After being treated with different concentrations of ethyl acetate extracts, the percentage of G0/G1 phase increased significantly, cyclin D1 and cyclin E expression decreased, apoptosis rate was significantly higher, and the ability of migration of PC-3 and RV1 was inhibited significantly. Western blot showed that the expressions of Bcl-2, p-ERK, and p-AKT were significantly decreased, but the expressions of Bax and caspase-3 cleavage were increased. Conclusions. Impatiens balsamina L. inhibited the proliferation of human prostate cancer cells; ethyl acetate extracts have the strongest effect. It could inhibit cell proliferation and migration, cause G1 phase arrest, and induce apoptosis probably through inhibition of the AKT and ERK pathways.

scholarly journals Inhibition of Prostate Cancer Cell Growth by Human Secreted PDZ Domain-Containing Protein 2, a Potential Autocrine Prostate Tumor Suppressor

Endocrinology ◽  
2006 ◽  
Vol 147 (11) ◽  
pp. 5023-5033 ◽  
Author(s):  
C. W. Tam ◽  
A. S. Cheng ◽  
R. Y. M. Ma ◽  
K.-M. Yao ◽  
S. Y. W. Shiu
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Caspase 3 ◽  
Pdz Domain ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Lncap Cells ◽  
Prostate Cancer Cells ◽  
Autocrine Growth ◽  
Endogenous Expression

A possible role of the PDZ domain-containing protein 2 (PDZD2) in prostate tumorigenesis has been suggested. Besides, PDZD2 is posttranslationally cleaved by a caspase-dependent mechanism to form a secreted PDZ domain-containing protein 2 (sPDZD2) with unknown functions in humans. In this study, we demonstrate the endogenous expression of PDZD2 and secretion of sPDZD2 in cancerous DU145, PC-3, 22Rv1, LNCaP, and immortalized RWPE-1 prostate epithelial cells. Inhibition of endogenous sPDZD2 production and secretion by DU145, PC-3, 22Rv1, and RWPE-1 cells via the caspase-3 inhibitor Z-DEVD-FMK resulted in increased cell proliferation, which was abrogated by treatment with exogenous recombinant sPDZD2. Whereas sPDZD2-induced antiproliferation in DU145, PC-3, and 22Rv1 cells, it induced apoptosis in LNCaP cells. The data suggest that endogenous sPDZD2, produced by caspase-3-mediated cleavage from PDZD2, may function as a novel autocrine growth suppressor for human prostate cancer cells. The antiproliferative effect of sPDZD2 was apparently mediated through slowing the entry of DU145, PC-3, and 22Rv1 cells into the S phase of the cell cycle. In DU145 cells, this can be attributed to stimulated p53 and p21CIP1/WAF1 expression by sPDZD2. On the other hand, the apoptotic effect of sPDZD2 on LNCaP cells was apparently mediated via p53-independent Bad stimulation. Together our results indicate the presence of p53-dependent and p53-independent PDZD2/sPDZD2 autocrine growth suppressive signaling pathways in human prostate cancer cells and suggest a novel therapeutic approach of harnessing the latent tumor-suppressive potential of an endogenous autocrine signaling protein like sPDZD2 to inhibit prostate cancer growth.

scholarly journals E2F site in the essential promoter region does not confer S phase-specific transcription of the ABCC10 gene in human prostate cancer cells

2017 ◽  
Vol 64 (2) ◽  
Author(s):  
Magdalena Dabrowska ◽  
Francis M. Sirotnak
Keyword(s):  
Prostate Cancer ◽  
Mrna Level ◽  
S Phase ◽  
Human Prostate ◽  
G1 Phase ◽  
Sequence Motif ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Cellular Detoxification ◽  
Binding Sequence

ABCC10 (MRP7) plays a role in cellular detoxification and resistance to anticancer drugs. Since ABCC10 gene transcription in human prostate cancer CWR22Rv1 cells was found dependent on E2F binding sequence motif, ABCC10 expression in G1 and S phases of the cell cycle of CWR22Rv1 cells, was analyzed. The cells were synchronized in G1 phase by double thymidine block and in S phase by thymidine/mimosine double block. ABCC10 mRNA level was found to be similar in S phase-synchronized and asynchronous cell populations. In G1 phase it decreased by 2.4- to 3-fold. It is thus inferred, that ABCC10 expression in CWR22Rv1 cells is not S phase-specific but is primarily associated with cell proliferation.

scholarly journals Rutin, a Flavonoid That Is a Main Component ofSaussurea involucrata, Attenuates the Senescence Effect in D-Galactose Aging Mouse Model

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Ying-Chen Yang ◽  
Hsueh-Yi Lin ◽  
Kang-Yi Su ◽  
Chien-Hsu Chen ◽  
Yung-Lung Yu ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Caspase 3 ◽  
Ethyl Acetate Extract ◽  
Avoidance Task ◽  
Behavioral Performance ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Saussurea Involucrata ◽  
Nf Kappab ◽  
Main Component

Saussurea involucrata(Kar. et Kir.), known as the snow lotus, grows in the Tian Shan and A’er Tai areas of China. It has recently been reported that the ethyl acetate extract ofS. involucrata(SI-2) can inhibit proliferation and induce apoptosis in PC-3 human prostate cancer cells. This study investigated the protective effect of ethyl acetate extract ofS. involucrata(SI-2) or rutin, a flavonoid extracted from ethyl acetate extract ofS. involucrata(SI-2), on D-galactose- (D-gal-) induced brain injury in mice. Administering SI-2 or rutin (30 mg/kg/d and 30 mg/kg/d) for 6 weeks, concomitant with D-gal injection, significantly increased superoxide dismutase and glutathione peroxidase activities and decreased the MDA level in plasma. Furthermore, the result showed that the percentages of cleaved caspase-3 and PARP in the D-gal-treated mice were much higher than those in the control. Pretreatment using SI-2 or rutin decreased the expression of cyclooxygenase-2 via downregulation of NF-kappaB, resulting in a decrease in lipid peroxidation. Furthermore, our results also showed that oral administration of rutin to these mice significantly improved behavioral performance in a step-through passive avoidance task and these results suggest that SI-2 or rutin exerts potent antiaging effects on D-gal in mice via antioxidative mechanisms.

Sign in / Sign up

Export Citation Format

Share Document