scholarly journals Anti-Inflammatory Activity of the Essential Oil Citral in Experimental Infection withStaphylococcus aureusin a Model Air Pouch

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Hellen Braga Martins ◽  
Nathan das Neves Selis ◽  
Clarissa Leal Silva e Souza ◽  
Flávia S. Nascimento ◽  
Suzi Pacheco de Carvalho ◽  
...  
Keyword(s):  
Essential Oil ◽  
Blood Cells ◽  
Mutual Influence ◽  
White Blood Cells ◽  
Local Treatment ◽  
Inflammatory Activity ◽  
Air Pouch ◽  
Qpcr Array ◽  
Anti Inflammatory ◽  
Air Pouch Model

This study proposes to implement an alternative and effective strategy for local treatment of disease provoked byS. aureus. For the analysis of possible anti-inflammatory activity of essential oil, after establishing an air pouch model, 48 male mice of Balb/c were treated, infected, and euthanized at 4 and 8 h. Thus, the total and differential white blood cells were counted in the animal’s blood, and cytokines IL-1β, IL-6, and TNF-αwere titrated using ELISA in the air pouch lavage. Moreover, TNF-α, IL-1β, and IL-6 gene expression was analyzed through an RT-qPCR array, andS. aureuswas quantified using qPCR. Our results,p<0.05, showed that EOC reduced the quantity of microorganisms. The group of mice treated with essential oil citral showed a significant decrease in TNF-αlevels in tests demonstrating anti-inflammatory activity. There is no data about the mutual influence of the air pouch model, essential oil citral, andS. aureus. Thus, considering the interaction of these variables and the anti-inflammatory activity of the essential oil citral, we demonstrated, by alternative local treatment, a new antimicrobial agent that is not an antibiotic.

Investigations of Anti-Inflammatory Activity of a Peptide-Based Hydrogel Using Rat Air Pouch Model

2018 ◽  
Vol 11 (3) ◽  
pp. 2849-2859 ◽  
Author(s):  
Pramod K. Gavel ◽  
Hamendra S. Parmar ◽  
Versha Tripathi ◽  
Narendra Kumar ◽  
Ankan Biswas ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Air Pouch ◽  
Anti Inflammatory ◽  
Air Pouch Model

scholarly journals Chemical Composition of Pinus brutia Ten Essential Oil and Its in vitro Anti-Inflammatory Activity

2020 ◽  
pp. 112-121
Author(s):  
Lina Karrat ◽  
Ream Nayal ◽  
Mohammad Yaser Abajy
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Pinus Brutia ◽  
Human Red Blood Cells ◽  
Anti Inflammatory ◽  
Stabilization Assay

This investigation aims to determine the chemical composition of Pinus brutia leaves essential oil and evaluate its anti-inflammatory property using Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay. The chemical composition of essential oil (EO) obtained by hydro-distillation of leaves of Pinus brutia was investigated by GC-MS. The anti-inflammatory effect of EO was evaluated using Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay. The main constituents of EO were α-Terpineol (66.16%), 3-Carene (4.90%), Carveol (4.55%) and cis-Verbenol (3.22%). The inhibion of hemolysis was observed at concentrations (2.5-12.5) µg/ml. Moreover, albumin denaturation test showed protection effect at concentrations (8-40) µg/ml. We concluded that, Pinusbrutia EO shows strong anti-inflammatory activity at different concentration when compared to standard drug of Diclofenac sodium. In addition, GC-MS analysis of Pinus brutia EO showed the presence of α-Terpineol as major compound in the oil. It reveals that this constituent is responsible to maximum protection of albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory by in vivo models.

scholarly journals Suppressions of Serotonin-Induced Increased Vascular Permeability and Leukocyte Infiltration byBixa orellanaLeaf Extract

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Yoke Keong Yong ◽  
NurShahira Sulaiman ◽  
Muhammad Nazrul Hakim ◽  
Gwendoline Ee Cheng Lian ◽  
Zainul Amirudin Zakaria ◽  
...  
Keyword(s):  
Vascular Permeability ◽  
Inflammatory Activity ◽  
Blue Dye ◽  
Leukocyte Infiltration ◽  
Permeability Model ◽  
Air Pouch ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Pharmacological Screening ◽  
Air Pouch Model

The aim of the present study was to evaluate the anti-inflammatory activities of aqueous extract ofBixa orellana(AEBO) leaves and its possible mechanisms in animal models. The anti-inflammatory activity of the extract was evaluated using serotonin-induced rat paw edema, increased peritoneal vascular permeability, and leukocyte infiltrations in an air-pouch model. Nitric oxide (NO), indicated by the sum of nitrites and nitrates, and vascular growth endothelial growth factor (VEGF) were measured in paw tissues of rats to determine their involvement in the regulation of increased permeability. Pretreatments with AEBO (50 and 150 mg kg−1) prior to serotonin inductions resulted in maximum inhibitions of 56.2% of paw volume, 45.7% of Evans blue dye leakage in the peritoneal vascular permeability model, and 83.9% of leukocyte infiltration in the air-pouch model. 57.2% maximum inhibition of NO and 27% of VEGF formations in rats’ paws were observed with AEBO at the dose of 150 mg kg−1. Pharmacological screening of the extract showed significant (P<0.05) anti-inflammatory activity, indicated by the suppressions of increased vascular permeability and leukocyte infiltration. The inhibitions of these inflammatory events are probably mediated via inhibition of NO and VEGF formation and release.

Anti-inflammatory activity of essential oil of an endemicThymus fontanesiiBoiss. & Reut. with chemotype carvacrol, and its healing capacity on gastric lesions

2017 ◽  
Vol 41 (3) ◽  
pp. e12359 ◽  
Author(s):  
Lilia Mouhi ◽  
Houria Moghrani ◽  
Noureddine Nasrallah ◽  
Abdeltif Amrane ◽  
Rachida Maachi
Keyword(s):  
Essential Oil ◽  
Inflammatory Activity ◽  
Gastric Lesions ◽  
Anti Inflammatory

ALLIUM SATIVUM ESSENTIAL OIL (ASEO);

2017 ◽  
Vol 24 (04) ◽  
pp. 612-616
Author(s):  
Faisal Irshad ◽  
Hina Mawani ◽  
Sana Naz
Keyword(s):  
Essential Oil ◽  
Blood Cell ◽  
Blood Cells ◽  
Allium Sativum ◽  
White Blood Cells ◽  
Albino Rats ◽  
Serum Triglycerides ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Animal House

Objectives: To determine the effects of Allium sativum essential oil (ASEO)phytotherapy on serum triglycerides, total cholesterol, HDLc, LDLc and blood cell counts inalbino rat model. Study design: Experimental study. Setting and Duration: Animal House,Sindh Agriculture University and Isra University Hyderabad from May 2014 to January 2015.Materials and Methods: 60 albino rats were divided into four groups. Controls were givenPlacebo. Experimental rat groups were given ASEO 100 mg/kg, 200 mg/kg and 300 mg/kgorally for 30 days. Cardiac puncture was performed for blood sampling. Research variableswere analyzed on Statistix 10.0 (USA). Results: Blood lipids showed significant reduction invarious blood lipid fractions. Serum LDLc exhibited with a concomitant rise in serum HDLc (p=0.0001) in high ASEO treated rats. Red blood cells, white blood cells and platelet showedsignificant improvement ASEO fed rats (p=0.001). Conclusion: Allium sativum essential oil(ASEO) phytotherapy showed a rise in HDLc and a reduction in LDLc, triglycerides and totalcholesterol with improvement in red blood cell counts.

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