scholarly journals Chemical Composition of Pinus brutia Ten Essential Oil and Its in vitro Anti-Inflammatory Activity

2020 ◽  
pp. 112-121
Author(s):  
Lina Karrat ◽  
Ream Nayal ◽  
Mohammad Yaser Abajy
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Pinus Brutia ◽  
Human Red Blood Cells ◽  
Anti Inflammatory ◽  
Stabilization Assay

This investigation aims to determine the chemical composition of Pinus brutia leaves essential oil and evaluate its anti-inflammatory property using Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay. The chemical composition of essential oil (EO) obtained by hydro-distillation of leaves of Pinus brutia was investigated by GC-MS. The anti-inflammatory effect of EO was evaluated using Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay. The main constituents of EO were α-Terpineol (66.16%), 3-Carene (4.90%), Carveol (4.55%) and cis-Verbenol (3.22%). The inhibion of hemolysis was observed at concentrations (2.5-12.5) µg/ml. Moreover, albumin denaturation test showed protection effect at concentrations (8-40) µg/ml. We concluded that, Pinusbrutia EO shows strong anti-inflammatory activity at different concentration when compared to standard drug of Diclofenac sodium. In addition, GC-MS analysis of Pinus brutia EO showed the presence of α-Terpineol as major compound in the oil. It reveals that this constituent is responsible to maximum protection of albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory by in vivo models.

Synthesis, Anti-Arthritic, and Anti-Inflammatory Activity of N-Tosyl aza Cyclophanes

2012 ◽  
Vol 65 (2) ◽  
pp. 186 ◽  
Author(s):  
Perumal Rajakumar ◽  
Ramar Padmanabhan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Blood Cells ◽  
Bovine Serum ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Human Red Blood Cells ◽  
Anti Inflammatory

The synthesis of novel N-tosyl tetraaza cyclophanes and N-tosyl diaza cyclophane incorporating m-terphenyl as spacer units is described. Anti-arthritic activity was studied by inhibition of the protein denaturation method (bovine serum albumin). All the N-tosyl aza cyclophanes exhibit excellent anti-arthritic activity. Anti-inflammatory activity of the synthesized cyclophanes was investigated using the human red blood cells (HRBC) membrane stabilization method and some of the N-tosyl aza cyclophanes exhibited good anti-inflammatory activity.

scholarly journals In VitroEffect ofCinnamomum zeylanicumBlume Essential Oil onCandidaspp. Involved in Oral Infections

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Marianne de Lucena Rangel ◽  
Sabrina Garcia de Aquino ◽  
Jefferson Muniz de Lima ◽  
Lúcio Roberto Castellano ◽  
Ricardo Dias de Castro
Keyword(s):  
Cell Wall ◽  
Essential Oil ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Fungal Growth ◽  
Human Cells ◽  
Phytochemical Analysis ◽  
Oral Infections ◽  
Human Red Blood Cells ◽  
Main Component

The present study demonstrates the antifungal potential of chemically characterized essential oil (EO) ofCinnamomum zeylanicumBlume onCandidaspp. biofilm and establishes its mode of action, effect on fungal growth kinetics, and cytotoxicity to human cells. The minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) values varied from 62.5 to 1,000μg/mL, and the effect seems to be due to interference with cell wall biosynthesis. The kinetics assay showed that EO at MICx2 (500μg/mL) induced a significant (p < 0.05) reduction of the fungal growth after exposure for 8 h. At this concentration, the EO was also able to hinder biofilm formation and reduceCandidaspp. monospecies and multispecies in mature biofilm at 24 h and 48 h (p < 0.05). A protective effect on human red blood cells was detected with the EO at concentrations up to 750μg/mL, as well as an absence of a significant reduction (p > 0.05) in the viability of human red blood cells at concentrations up to 1,000μg/mL. Phytochemical analysis identified eugenol as the main component (68.96%) of the EO.C. zeylanicumBlume EO shows antifungal activity, action on the yeast cell wall, and a deleterious effect onCandidaspp. biofilms. This natural product did not show evidence of cytotoxicity toward human cells.

scholarly journals Chemical composition of PM10 and its effect on in vitro hemolysis of human red blood cells (RBCs): a comparison study during dust storm and inversion

2019 ◽  
Vol 17 (1) ◽  
pp. 493-502 ◽  
Author(s):  
Maryam Faraji ◽  
Zahra Pourpak ◽  
Kazem Naddafi ◽  
Ramin Nabizadeh Nodehi ◽  
Mohammad Hossein Nicknam ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Red Blood Cells ◽  
Dust Storm ◽  
Blood Cells ◽  
Comparison Study ◽  
Human Red Blood Cells ◽  
And Inversion

scholarly journals POTENTIAL ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTIBACTERIAL EVALUATION OF EXTRACTS OF LEUCAS ASPERA USING IN VITRO MODELS

2016 ◽  
Vol 8 (12) ◽  
pp. 292 ◽  
Author(s):  
Tahareen S. ◽  
Shwetha R. ◽  
Myrene R. D.
Keyword(s):  
Inflammatory Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Leucas Aspera ◽  
Ic50 Value ◽  
Anti Inflammatory ◽  
Stabilization Assay

<p><strong>Objective: </strong>To evaluate the potential antioxidant, anti-inflammatory and antibacterial activities of aqueous and methanolic extracts of leaves of <em>Leucas aspera</em> (Thumbae).</p><p><strong>Methods: </strong>Phytochemical screening of the leaves of <em>L. aspera</em> was followed by analysis of antioxidant activity by means of DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activity. <em>In vitro</em> anti‐inflammatory activity was evaluated using lipoxygenase inhibition, albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity at different concentrations. Aspirin was used as a standard drug for the study of anti‐inflammatory activity. Linear regression analysis was used to calculate half maximal inhibitory concentration, IC50 value. The zone of inhibition was performed against common pathogens to determine the antimicrobial activity at different concentrations of plant extracts (60%, 70%, 80%).</p><p><strong>Results: </strong>The phytochemical analysis revealed the presence of carbohydrates, amino acid, alkaloids, tannins, flavonoids, glycosides, xanthoproteins, and phenols. The total phenolic and flavonoid content was found to be 2.25±0.04 mg GAE/g (gallic acid equivalents) and 1.2±0.05 mg QE/g (Quercetin equivalents) of fresh weight tissue respectively. The IC50 values for hydrogen peroxide scavenging activity were found to be 244.6 µg/ml. The extract inhibited the lipoxygenase enzyme activity with an IC50 value of 356.3 µg/ml. Maximum inhibition of heat-induced protein denaturation of 69% was observed at 400 μg/ml, IC50 249.6 μg/ml. Proteinase activity was also significantly inhibited (IC50 = 421.6 μg/ml). Membrane stabilization assay attributed minor protection by the leaf extract with an IC50 of 206.7. It was observed that <em>E. coli</em> were inhibited at all concentrations, followed by <em>Klebsiella</em> and <em>Pseudomonas</em>.</p><p><strong>Conclusion: </strong>Results indicate that L. aspera possess anti-inflammatory properties due to the strong occurrence of polyphenolic compounds such as alkaloids, flavonoids, tannins and steroids that serve as free radical inhibitors or scavenger. Compounds of the plant L. aspera may hence be used as lead compounds for designing potent anti-inflammatory drug which can be used for treatment of various diseases.</p><p> </p>

Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats

2021 ◽  
Vol 169 ◽  
pp. 105105
Author(s):  
Marielba de los Ángeles Rodríguez Salazar ◽  
Glides Rafael Olivo Urbina ◽  
Vânia Maria Borges Cunha ◽  
Fernanda Wariss Figueiredo Bezerra ◽  
Michelle Nerissa Coelho Dias ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Cytotoxic Effect ◽  
Adult Rats ◽  
Human Red Blood Cells ◽  
Cord Injury ◽  
Anti Inflammatory

scholarly journals STUDY OF PARMELIA PERLATA FOR ITS POTENTIAL AS ANTI-INFLAMMATORY AND ANTIARTHRITIC AGENT USING IN VITRO MODEL

2019 ◽  
Vol 12 (1) ◽  
pp. 95 ◽  
Author(s):  
Yogesh Diwakar ◽  
Chitra V ◽  
Evelyn Sharon S
Keyword(s):  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Maximum Effect ◽  
Dependent Manner ◽  
Membrane Stabilization ◽  
Human Red Blood Cells ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Vitro Model

Objective: The objective of this study was to evaluate the anti-inflammatory and antiarthritic potential of Parmelia perlata. Methods: The relative study is based on in vitro anti-inflammatory and antiarthritic activity using hydroalcoholic extract of P. perlata (HAEPP). The preliminary phytochemical tests showed the presence of various phytochemical compounds such as alkaloids, flavonoids, and glycosides since the lichen species of P. perlata has the folklore claim of anti-inflammatory activity, thus it was studied by human red blood cells membrane stabilization method, and arthritic activity was carried using protein denaturation method using diclofenac as a standard.Results: The results showed eminent anti-inflammatory and antiarthritic activity in a dose-dependent manner. The membrane stabilization showed the maximum effect at 78.54% at the concentration of 1000 μg/, and the protein denaturation was also found maximum at 1000 μg/ml concentration at 79.43%. Thus, our research states the potent anti-inflammatory activity and antiarthritic effect in P. perlata. Conclusion: The HEAPP has a potent anti-inflammatory activity and antiarthritic activity. A further study has to be conducted to establish the pharmacological evidence behind the compound and the mechanism of action of the HAEPP on the inhibition of the inflammation process.

Anti-inflammatory Activity and Chemical Composition of Essential Oil Extracted with Supercritical CO2from the Brown SeaweedUndaria pinnatifida

2016 ◽  
Vol 19 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Ji-Young Kang ◽  
Byung-Soo Chun ◽  
Min-Chul Lee ◽  
Jae-Suk Choi ◽  
In Soon Choi ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Inflammatory Activity ◽  
Anti Inflammatory

Anti-inflammatory activity and chemical composition of aqueous extract and essential oil from leaves of Ocimum selloi Benth.

2021 ◽  
Vol 275 ◽  
pp. 114136
Author(s):  
Raul Cremonezi Piva ◽  
Maria Helena Verdan ◽  
Lidiane Schultz Branquinho ◽  
Cândida Aparecida Leite Kassuya ◽  
Claudia Andrea Lima Cardoso
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Aqueous Extract ◽  
Inflammatory Activity ◽  
Anti Inflammatory
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