scholarly journals Distribution and density of conjunctival goblet cells

2002 ◽  
Vol 55 (5-6) ◽  
pp. 195-200 ◽  
Author(s):  
Vesna Vujkovic ◽  
Gostimir Mikac ◽  
Risto Kozomara
Keyword(s):  
Cell Density ◽  
Dry Eye ◽  
Goblet Cell ◽  
Tear Film ◽  
Goblet Cells ◽  
Dry Eye Syndrome ◽  
Bulbar Conjunctiva ◽  
Cell Distribution ◽  
Significant Difference ◽  
Goblet Cell Density

There is a regional variation in conjunctival goblet cell distribution and density per unit of measurement. By secreting mucin goblet cells are involved in formation of tear film. Tear film instability is the basic sign of dry eye syndrome. Although dry eye syndrome can be a side effect of a disease, the most common cause is normal aging. The objective of the research was to determine goblet cell density in bulbar and lower forniceal conjunctiva and possible changes in goblet cell density related to age and sex. The research was conducted on 30 conjunctival samples of patients with no clinical changes of the conjunctiva. Tissue sections were stained with hematoxylin eosin (HE), periodic acid-schiff (PAS), alcian blue (AB)/PAS Giemsa methods. Goblet cell density was 1.24?1.62 in bulbar conjunctiva and 30.21?14.32 in lower forniceal conjunctiva. Goblet cell distribution in the conjunctiva was unequal. A correlation between goblet cell density and age was not determined. Goblet cell density doesn't decrease with aging. No significant difference in goblet cell density between men and women was established.

scholarly journals The conjunctival impression cytology between the diagnosed cases of dry eye and normal individuals

1970 ◽  
Vol 3 (1) ◽  
pp. 39-44 ◽  
Author(s):  
E Shresta ◽  
JK Shrestha ◽  
G Shayami ◽  
M Chaudhary
Keyword(s):  
Cell Density ◽  
Dry Eye ◽  
Goblet Cell ◽  
Periodic Acid ◽  
Dry Eye Syndrome ◽  
Bulbar Conjunctiva ◽  
Impression Cytology ◽  
Conjunctival Impression Cytology ◽  
Normal Individuals ◽  
Goblet Cell Density

Background: The dry eye or tear film dysfunction is a common ophthalmic syndrome. Objective: To compare the results of conjunctival impression cytology between dry eye patients and normal individuals.Subjects and methods: A case control study including consecutive cases of dry eye syndrome was carried out. Individuals without dry eye were taken as control. Impression of conjunctiva with cellulose acetate filter paper was taken from inferonasal bulbar conjunctiva and was stained with Periodic Acid- Schiff (PAS) and counter-stained with haematoxylin and eosin. Main outcome measure: goblet cell density. Results: There was a female preponderance in dry eye disease. Of 114 dry eye cases, 49.2% eyes showed decreased or absent goblet cell density. In 72 normal individuals 73.7% eyes showed normal goblet cell density and 26.3% of eyes showed decreased or absent goblet cells (p < 0.001). The tear break-up time (TBUT) test was significantly more likely to be less than 10 seconds in cases as compared to the controls ( OR = 19.36, 95% CI = 7.56 – 52.52). Similarly, the goblet cell density was likely to be significantly reduced in cases with dry eye syndrome (OR= 2.25, 95% CI = 1.26 – 4.02, p = 0.003). Conclusion: Goblet cell density significantly reduces in dry eye syndrome. The impression cytology is a useful test for the diagnosis of dry eye syndrome. Key words: impression cytology; conjunctiva; dry eyes; tear filmDOI: 10.3126/nepjoph.v3i1.4277Nepal J Ophthalmol 2011;3(5):39-44

scholarly journals Effect of persimmon leaves (Diospyros kaki) on goblet cell density and inflammation in experimental dry eye model

2020 ◽  
Vol 63 (1) ◽  
Author(s):  
Kyung-A Kim ◽  
Chung Hyun Lee ◽  
Tae Kyeom Kang ◽  
Sung Jae Yang ◽  
Chang Yong Lee ◽  
...  
Keyword(s):  
Cell Density ◽  
Dry Eye ◽  
Goblet Cell ◽  
Diospyros Kaki ◽  
Eye Model ◽  
Goblet Cell Density

scholarly journals Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis Patients

2018 ◽  
Vol 2018 ◽  
pp. 1-11
Author(s):  
Marcella Nebbioso ◽  
Marta Sacchetti ◽  
Guia Bianchi ◽  
Anna Maria Zicari ◽  
Marzia Duse ◽  
...  
Keyword(s):  
Cell Density ◽  
Goblet Cell ◽  
Ocular Surface ◽  
Goblet Cells ◽  
Significant Alteration ◽  
Vernal Keratoconjunctivitis ◽  
Eye Drops ◽  
Before And After ◽  
Tear Ferning Test ◽  
Goblet Cell Density

Background. Vernal keratoconjunctivitis (VKC) is a rare ocular surface inflammatory disease that affects mainly boys in the first decade of life. Clinical observations show that it generally regresses spontaneously with the onset of puberty, but therapeutic measures must be taken before then to control the course of the disease. Purpose.To evaluate the role of the lacrimal mucous component in VKC patients and compare tear ferning test (TFT) modifications, MUC5AC levels in tears, and density of conjunctival goblet cells to clinical characteristics before and after treatment with cyclosporine A (CY) in eye drops. Methods. Forty-seven patients affected by VKC and 30 healthy subjects aged between 3 and 16 years of life were enrolled. All individuals were submitted to complete eye examination and skin prick test (SPT) for the most common allergens. Then, they were subjected to collection of the tears and to impression cytology to evaluate TFT, MUC5AC levels, and conjunctival goblet cell density, before and after treatment with CY in eye drops. Results. Comparing the VKC group vs. the control group at baseline, a significant alteration in the degree of the ferns was found, indicating a pathological condition of the lacrimal mucous layer. In addition, an increased number of goblet cells were observed in the patients. The concentration of lacrimal secretory mucins (MUC5AC) did not show significant differences between the 2 groups. Patients treated with CY have reported improvements of some signs and symptoms of disease activity, including TFT, and a tendency of conjunctival goblet cell density to normalise. Conclusions. The results obtained demonstrated for the first time a significant alteration of the lacrimal mucin component evaluated in the VKC group, and an improvement of the latter after CY therapy.

Distribution and Density of Goblet Cells in the Normal Adult Human Middle Ear

1973 ◽  
Vol 82 (2) ◽  
pp. 240-247 ◽  
Author(s):  
K. Bak-Pedersen ◽  
M. Tos
Keyword(s):  
Cell Density ◽  
Middle Ear ◽  
Goblet Cell ◽  
Anterior Part ◽  
Goblet Cells ◽  
Mastoid Process ◽  
Clinically Normal ◽  
Normal Series ◽  
Temporal Bones ◽  
Goblet Cell Density

The purpose of this study was to map the distribution and density of goblet cells in the clinically normal middle ear. From 12 temporal bones, derived from 12 adult patients with clinically normal middle ears, the mucosa was prepared and stained by the PAS-alcian blue whole-mount method. In six of the patients the middle ears with meticulous gross and microscopic investigation showed mild sequelae of previous otitis media, called the slightly abnormal series, whereas the other six patients who had entirely normal middle ears were designated the normal series. Each middle ear was investigated in 12 different areas: tympanic orifice, hypotympanum anteriorly and posteriorly, round window niche, oval window niche, promontory anteriorly, in the middle, and posteriorly, epitympanum, antrum, and mastoid process. In each middle ear area the goblet-cell density and distribution were determined on the basis of 30 to 50 counts of 0.01768 mm2 epithelial surface, a total of at least 4800 counts. In six patients goblet cells were found in all 12 areas, in three patients in 11, and in three patients in 10. In both series there was a distinct decrease in goblet-cell density from the tympanic orifice, towards the posterior areas of the middle ear and the mastoid process. However, two patients of the slightly abnormal series exhibited a considerably greater density in the posterior than in the anterior part of the hypotympanum. The mean goblet-cell density in the slightly abnormal series was distinctly greater in all middle ear areas than in the normal series. Correspondingly, goblet cells were demonstrated in the antrum and mastoid process in all patients of the slightly abnormal series, but in only half of the normal series. Therefore, the greater goblet-cell density in the slightly abnormal series presumaby results from a previous past disease condition of the middle ear.

scholarly journals Stimulation of Basal Secretion in the Treatment of Dry Eye Syndrome

2019 ◽  
Vol 16 (1) ◽  
pp. 70-75
Author(s):  
L. I. Uchakova ◽  
S. V. Balalin ◽  
V. L. Zagrebin
Keyword(s):  
Dry Eye ◽  
Tear Film ◽  
Goblet Cells ◽  
Dry Eye Syndrome ◽  
Ophthalmological Examination ◽  
Basal Secretion ◽  
Tear Production ◽  
Before And After ◽  
The Stability ◽  
Conjunctival Goblet Cells

The aim is to evaluate the effectiveness of Stillavit in the treatment of dry eye syndrome (DES) and its effect on the density of conjunctival goblet cells and the functional parameters of tear production. Methods. There were analyzed treatment results of 18 patients (36 eyes) with DES at the age of 48 to 80 years (average 60.6 ± 1.3 years) including 17 women and 1 male before and after 1 and 3 months after Stillavit prescription. In all patients in addition to a standard ophthalmological examination the results of Schirmer I, Norn, as well as the LIPCOF test were evaluated and the density of conjunctival goblet cells was examined. Results. After 1 and 3 months there was a significant improvement in the results of the Norn test: from 5.17 ± 0.22 seconds to 7.18 ± 0.17 seconds which indicated an increase in the stability of the tear film. After 3 months there was a significant increase in the density of conjunctival goblet cells, improvement in the Schirmer sample: from 9.26 ± 0.89 to 11.7 ± 0.6 mm (t = 2.28, p < 0.05) and LIPCOF test (t = 2.18, p < 0.05) which indicated an increase in tear production and a significant decrease in the inflammatory process and edema of the conjunctiva. Conclusion. Treatment of DES with the use of Stillavit leads after 3 months to a significant increase in the density of conjunctival goblet cells, increases tear production and stability of the tear film.

scholarly journals Effect of IRT5 probiotics on dry eye in the experimental dry eye mouse model

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243176
Author(s):  
Jayoon Moon ◽  
Jin Suk Ryu ◽  
Jun Yeop Kim ◽  
Sin-Hyeog Im ◽  
Mee Kum Kim
Keyword(s):  
Cell Density ◽  
Dry Eye ◽  
Goblet Cell ◽  
Intestinal Microbiome ◽  
Rt Pcr ◽  
Oral Gavage ◽  
Once Daily ◽  
Tear Secretion ◽  
Eye Model ◽  
Goblet Cell Density

Objective To investigate the clinical effects of IRT5 probiotics in the environmental dry eye model. Methods Eight week old male C57BL/6 mice were randomly divided into two groups; control group (n = 16) received oral gavage of 300 μL phosphate-buffered saline (PBS) alone once daily, IRT5 group (n = 9) received oral gavage of 1 x 109 CFU IRT5 probiotics powder in 300 μL PBS once daily, both groups for 11 to 12 days. Simultaneously, all mice underwent dry eye induction. Tear secretion, corneal staining and conjunctival goblet cell density were evaluated. Quantative real-time polymerase chain reaction (RT-PCR) for inflammation-related markers was performed. 16S ribosomal RNA of fecal microbiome was analyzed and compositional difference, alpha and beta diversities were assessed. Results There was no difference in NEI score but significant increase in tear secretion was observed in IRT5 group (p < 0.001). There was no significant difference in goblet cell density between groups. Quantative RT-PCR of cornea and conjunctiva revealed increased TNF-α expression in IRT5 group (p < 0.001) whereas other markers did not significantly differ from control. IRT5 group had significantly increased species diversity by Shannon index (p = 0.041). Beta diversity of genus by UniFrac principle coordinates analysis showed significant distance between groups (p = 0.001). Compositional differences between groups were observed and some were significantly associated with tear secretion. Multivariate linear regression analysis revealed Christensenellaceae (p = 0.009), Lactobacillus Helveticus group (p = 0.002) and PAC001797_s (p = 0.011) to strongly influence tear secretion. Conclusion In experimental dry eye model, IRT5 probiotics treatment partially improves experimental dry eye by increasing tear secretion which was associated with and influenced by the change in intestinal microbiome. Also, intestinal microbiome may affect the lacrimal gland through a different mechanism other than regulating inflammation.

Comparative Study between Dryness Post Phacoemulsification and Post Extra Capsular Cataract Extraction

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Mohammed Elgeshy Alm Eldin ◽  
Saad Mohammed Rashad ◽  
Ayman Mohamed Elsaka ◽  
Samah Mahmoud Fawzy ◽  
Mahmoud Ahmed ElSamkary
Keyword(s):  
Comparative Study ◽  
Cell Count ◽  
Dry Eye ◽  
Goblet Cell ◽  
Tear Film ◽  
Cataract Extraction ◽  
Significant Deterioration ◽  
Meniscus Height ◽  
Significant Difference ◽  
Break Up

Abstract Purpose This study aims to detect and compare the severity and pattern of dry eye occurrence after phacoemulsification and extra capsular cataract extraction surgeries. Methods Twenty eyes of twenty patients in two groups, ten eyes planned for Phacoemulsification(phaco) and ten eyes planned for Extra Capsular Cataract Extraction (ECCE) enrolled in a prospective comparative study. All patients underwent full history taking and full ophthalmological clinical evaluation including (tear film meniscus height, Schirmer 1 test, tear break up time). Evaluations will be done one week before cataract surgery then three and six weeks after the surgery.Also, goblet cell count is measured twice, before and 6 weeks after the operation. Results After both Phaco, andECCE, the changes between means of pre-operative, 3 weeks and 6 weeks visits were significant in tear film meniscus height (p = 0.01) and were insignificant in both Schirmer 1 and tear break up time (p &gt; 0.05). There was a significant deterioration after 3 weeks of surgery in tear film meniscus height followed by significant increase between 3rd and 6th weeks post-operative measurements (p = 0.01). There was a significant difference between the 2 groups in the total change of Schirmer 1 and tear break up time between the6th weeks and the pre-operative measure with more deterioration in group 2. There was no significant difference between the 2 groups in tear film meniscus height 6 weeks post operation.The deterioration in goblet cell count was significantly worse in the ECCE group (mean difference of -22.1±8.5 and was -7.5±5.1 in the phaco group), (p = 0.0005). Conclusions Phacoemulsification surgery may aggravate the signs and symptoms of dry eye and affect dry eye test values in patients in short-term but less than ECCE.

Goblet Cell Population in the Pathological Middle Ear and Eustachian Tube of Children and Adults

1977 ◽  
Vol 86 (2) ◽  
pp. 209-218 ◽  
Author(s):  
Mirko Tos ◽  
K. Bak-Pedersen
Keyword(s):  
Cell Density ◽  
Eustachian Tube ◽  
Middle Ear ◽  
Goblet Cell ◽  
Newborn Infants ◽  
Goblet Cells ◽  
Secretory Otitis Media ◽  
Temporal Bones ◽  
Different Parts ◽  
Goblet Cell Density

On 64 temporal bones from slightly abnormal and pathological prematures and newborn infants, children, and adults, 35,000–40,000 counts of goblet cells were done in different parts of the Eustachian tube and middle ear. The goblet cells were present in all Eustachian tubes and middle ear areas. In ears previously exposed to infection the density was slightly increased in the tubal orifice and in most parts of the middle ear. In subacute and acute pathological actions, especially tubal occlusion, and in chronic diseases, such as active chronic otitis and in secretory otitis media, the goblet-cell density is appreciably increased.

Scanning Electron Microscopy of Experimental Keratoconjunctivitis Sicca in Dogs: Cornea and Bulbar Conjunctiva

1988 ◽  
Vol 25 (6) ◽  
pp. 468-474 ◽  
Author(s):  
T. J. Kern ◽  
H. N. Erb ◽  
J. M. Schaedler ◽  
E. P. Dougherty
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Replacement Therapy ◽  
Cell Density ◽  
Goblet Cell ◽  
Keratoconjunctivitis Sicca ◽  
Goblet Cells ◽  
Dark Cell ◽  
Scanning Electron ◽  
Goblet Cell Density

Keratoconjunctivitis sicca was produced experimentally in 16 beagles by bilateral surgical removal of the lacrimal and nictitans glands; four dogs were not treated, and 12 received tear-replacement therapy on post-operative days 7 through 28. Keratoconjunctivitis sicca was verified by reduction in Schirmer tear test values by post-operative day 6, and there was no response on day 28 to tear-replacement therapy. Corneas of both normal and tear-deficient dogs had polygonal squamous epithelial cells of light and dark electron density by scanning electron microscopy. Light cells had more microvilli and microplicae than dark cells. Conjunctivae were similar to corneas, except for numerous goblet cells on the surface. Corneal dark-cell density and goblet cell density were not different between groups. Goblet cells most often occurred singly in normal dogs, while they were in clusters in tear-deficient dogs. A hypothesis that petrolatum/mineral oil ointment should provide more effective artificial tear replacement than hydroxymethylcellulose drops for tear-deficient dogs could not be confirmed by objective analysis of corneal dark-cell density or conjunctival goblet cell density.

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