scholarly journals A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaohua Ma ◽  
Guoguang Wu ◽  
Yuehua Zhao ◽  
Zibo Yuan ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Fluorescent Probe ◽  
High Selectivity ◽  
Coumarin Derivative ◽  
Living Cells ◽  
Sensitive Detection ◽  
Aqueous Environment ◽  
Test Probe ◽  
Aqueous Buffer ◽  
Turn On

We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable fluorescence off-on response (∼52-fold) by the reaction with Cys in 100% aqueous buffer. The sensing mechanism was verified by the HPLC test. Probe 1 also displays high selectivity towards Cys. The detection limit was calculated to be 23 nM. Moreover, cellular experiments demonstrated that the probe is highly biocompatible and can be used for monitoring intracellular Cys.

Colorimetric and Fluorescent Probe for Hypochlorite with High Selectivity

2013 ◽  
Vol 295-298 ◽  
pp. 475-478 ◽  
Author(s):  
Zhi Xiang Han ◽  
Ming Hui Du ◽  
Guo Xi Liang ◽  
Xiang Yang Wu
Keyword(s):  
Reactive Oxygen Species ◽  
Aqueous Solution ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
Rhodamine B ◽  
High Selectivity ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Turn On

Rhodamine B thiohydrazide (RBS) was firstly employed as turn-on fluorescent probe for hypochlorite in aqueous solution and living cells. It exhibits a stable response to hypochlorite from 1.0×10-6to 1.0×10-5M with a detection limit of 3.3×10-7M. The response of this probe to hypochlorite is fast and highly selective compared with other reactive oxygen species (such as.OH,1O2, H2O2) and other common anions (such as X-, ClO2-, ClO4-, NO3-, NO2-, OH-, Ac-, CO32-, SO42-).

Rhodamine-based “turn-on” fluorescent probe with high selectivity for Fe2+ imaging in living cells

2013 ◽  
Vol 439 (4) ◽  
pp. 459-463 ◽  
Author(s):  
Gui-Ge Hou ◽  
Chun-Hua Wang ◽  
Ju-Feng Sun ◽  
Mei-Zi Yang ◽  
Dong Lin ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
High Selectivity ◽  
Living Cells ◽  
Turn On

A ratiometric fluorescent probe for rapid and sensitive visualization of hypochlorite in living cells

RSC Advances ◽  
2014 ◽  
Vol 4 (81) ◽  
pp. 43110-43113 ◽  
Author(s):  
Jiayu Zha ◽  
Boqiao Fu ◽  
Caiqin Qin ◽  
Lintao Zeng ◽  
Xichao Hu
Keyword(s):  
Detection Limit ◽  
Fluorescent Probe ◽  
High Selectivity ◽  
Fast Response ◽  
Living Cells ◽  
Low Detection Limit ◽  
Colorimetric Response ◽  
Ratiometric Fluorescent Probe

A novel fluorescent probe displayed a colorimetric response to ClO− with high selectivity, fast response and a low detection limit (0.08 μM).

A novel colorimetric/fluorescence dual-channel sensor based on NBD for the rapid and highly sensitive detection of cysteine and homocysteine in living cells

2016 ◽  
Vol 8 (11) ◽  
pp. 2420-2426 ◽  
Author(s):  
Youming Shen ◽  
Xiangyang Zhang ◽  
Youyu Zhang ◽  
Chunxiang Zhang ◽  
Junling Jin ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Optical Sensing ◽  
Living Cells ◽  
Sensitive Detection ◽  
Turn On ◽  
Dual Channel ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A novel and simple turn-on fluorescent probe bearing NBD and benzaldehyde fragments for the rapid optical sensing of biothiols has been designed and synthesized.

Novel Nopinone-Based Turn-on Fluorescent Probe for Hydrazine in Living Cells with High Selectivity

2019 ◽  
Vol 58 (51) ◽  
pp. 22754-22762 ◽  
Author(s):  
Mingxin Li ◽  
Jinfa He ◽  
Zhonglong Wang ◽  
Qian Jiang ◽  
Haiyan Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
High Selectivity ◽  
Living Cells ◽  
Turn On

scholarly journals A new 3-hydroxyphthalimide-based turn-on fluorescent probe for Hg2+ detection in aqueous solution

2020 ◽  
Vol 44 (5-6) ◽  
pp. 349-353
Author(s):  
Xue-Tao Pan ◽  
Qiao Li ◽  
Yuan-Yuan Xu ◽  
Sheng-Li Hu
Keyword(s):  
Aqueous Solution ◽  
Excited State ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
High Selectivity ◽  
Intramolecular Proton Transfer ◽  
Recognition Mechanism ◽  
Turn On ◽  
Transfer Mechanisms ◽  
Analytical Detection

A new 3-hydroxyphthalimide-based turn-on fluorescent probe is designed and synthesized. This probe can be used to determine the presence of Hg2+ ions by fluorescence spectroscope with high selectivity over other metal ions in aqueous solution. The analytical detection limit for Hg2+ is as low as 6.5 × 10−7 M. The recognition mechanism is attributed to Hg2+-promoted carbonothioate group cleavage and a subsequent excited-state intramolecular proton transfer mechanisms.

A PET-based turn-on fluorescent probe for sensitive detection of thiols and H2S and its bioimaging application in living cells, tissues and zebrafish

2019 ◽  
Vol 43 (7) ◽  
pp. 2865-2869 ◽  
Author(s):  
Kaixin Xu ◽  
Longwei He ◽  
Yunzhen Yang ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Sensitive Detection ◽  
Two Photon ◽  
Turn On

A two-photon turn-on fluorescent probe regulated by a PET mechanism has been engineered for detection of thiols and H2S in living cells, tissues and zebrafish.

A colorimetric and ratiometric fluorescent probe for the rapid and sensitive detection of sulfite in sugar

2016 ◽  
Vol 8 (7) ◽  
pp. 1572-1576 ◽  
Author(s):  
Xiaoming Jiang ◽  
Junchao Xu ◽  
Youlai Zhang ◽  
Hongying Wang ◽  
Lintao Zeng ◽  
...  
Keyword(s):  
Detection Limit ◽  
Fluorescent Probe ◽  
High Selectivity ◽  
Fast Response ◽  
Sensitive Detection ◽  
Low Detection Limit ◽  
Ratiometric Fluorescent Probe

A colorimetric and ratiometric fluorescent probe shows a fast response and high selectivity towards sulfite with a low detection limit (12 nM).

A diketopyrrolopyrrole-based fluorescence turn-on probe for the detection of Pb2+in aqueous solution and living cells

RSC Advances ◽  
2016 ◽  
Vol 6 (57) ◽  
pp. 52004-52008 ◽  
Author(s):  
Xiaofeng Yang ◽  
Yan Zhang ◽  
Yexin Li ◽  
Xiaolei Liu ◽  
Jiaxin Mao ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Turn On ◽  
Low Detection Limit ◽  
Fluorescence Turn On

A diketopyrrolopyrrole-based fluorescent probeDPP-HBTbearing a benzothiazole hydrazone motif exhibited an obvious fluorescent turn-on response toward Pb2+ions with a low detection limit of 2.3 × 10−10M in aqueous solution.

The influences of different substituents on spectral properties of rhodamine B based chemosensors for mercury ion and application in EC109 cells

2017 ◽  
Vol 95 (7) ◽  
pp. 751-757 ◽  
Author(s):  
Wenqi Du ◽  
Yu Cheng ◽  
Weixin Shu ◽  
Baoying Wu ◽  
Zhineng Kong ◽  
...  
Keyword(s):  
Detection Limit ◽  
Spectral Properties ◽  
Laser Scanning ◽  
High Selectivity ◽  
Living Cells ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ratiometric Fluorescence ◽  
Turn On ◽  
Fluorescence Change

Six rhodamine-based “turn-on” fluorescence chemosensors (L1–L6) with different substituents for mercury (Hg2+) were readily synthesized and investigated; they displayed high selectivity and chelation enhanced ratiometric fluorescence change and colorimetric change with Hg2+ among the metal ions examined. Based on UV and fluorescence spectral data, the effects of different substituents on spectral properties of the probes were presented and discussed. The detection limit of Hg2+ to probe L1 was as low as 50 nmol/L because of its electron-donating group. Theoretical calculation also supported the process of reaction. Confocal laser scanning microscopy experiments showed that probe could be used to detect Hg2+ in living cells.

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