The influences of different substituents on spectral properties of rhodamine B based chemosensors for mercury ion and application in EC109 cells

2017 ◽  
Vol 95 (7) ◽  
pp. 751-757 ◽  
Author(s):  
Wenqi Du ◽  
Yu Cheng ◽  
Weixin Shu ◽  
Baoying Wu ◽  
Zhineng Kong ◽  
...  
Keyword(s):  
Detection Limit ◽  
Spectral Properties ◽  
Laser Scanning ◽  
High Selectivity ◽  
Living Cells ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ratiometric Fluorescence ◽  
Turn On ◽  
Fluorescence Change

Six rhodamine-based “turn-on” fluorescence chemosensors (L1–L6) with different substituents for mercury (Hg2+) were readily synthesized and investigated; they displayed high selectivity and chelation enhanced ratiometric fluorescence change and colorimetric change with Hg2+ among the metal ions examined. Based on UV and fluorescence spectral data, the effects of different substituents on spectral properties of the probes were presented and discussed. The detection limit of Hg2+ to probe L1 was as low as 50 nmol/L because of its electron-donating group. Theoretical calculation also supported the process of reaction. Confocal laser scanning microscopy experiments showed that probe could be used to detect Hg2+ in living cells.

Colorimetric and Fluorescent Probe for Hypochlorite with High Selectivity

2013 ◽  
Vol 295-298 ◽  
pp. 475-478 ◽  
Author(s):  
Zhi Xiang Han ◽  
Ming Hui Du ◽  
Guo Xi Liang ◽  
Xiang Yang Wu
Keyword(s):  
Reactive Oxygen Species ◽  
Aqueous Solution ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
Rhodamine B ◽  
High Selectivity ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Turn On

Rhodamine B thiohydrazide (RBS) was firstly employed as turn-on fluorescent probe for hypochlorite in aqueous solution and living cells. It exhibits a stable response to hypochlorite from 1.0×10-6to 1.0×10-5M with a detection limit of 3.3×10-7M. The response of this probe to hypochlorite is fast and highly selective compared with other reactive oxygen species (such as.OH,1O2, H2O2) and other common anions (such as X-, ClO2-, ClO4-, NO3-, NO2-, OH-, Ac-, CO32-, SO42-).

scholarly journals A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaohua Ma ◽  
Guoguang Wu ◽  
Yuehua Zhao ◽  
Zibo Yuan ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Fluorescent Probe ◽  
High Selectivity ◽  
Coumarin Derivative ◽  
Living Cells ◽  
Sensitive Detection ◽  
Aqueous Environment ◽  
Test Probe ◽  
Aqueous Buffer ◽  
Turn On

We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable fluorescence off-on response (∼52-fold) by the reaction with Cys in 100% aqueous buffer. The sensing mechanism was verified by the HPLC test. Probe 1 also displays high selectivity towards Cys. The detection limit was calculated to be 23 nM. Moreover, cellular experiments demonstrated that the probe is highly biocompatible and can be used for monitoring intracellular Cys.

scholarly journals Current opportunities and prospects of confocal laser scanning microscopy in morphological research (review)

2021 ◽  
pp. 135-143
Author(s):  
N. N. Dorofienko ◽  
I. А. Аndrievskaya ◽  
О. A. Udovichenko
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Scientific Literature ◽  
Three Dimensional ◽  
Living Cells ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Morphological Studies

Introduction. The review discloses the results of scientific works related to a modern high-tech method for conducting morphological studies in biology and medicine – confocal laser scanning microscopy. This method, in combination with immunofluorescence histochemistry, can be used in a variety of studies: from rapid visualization of dynamic processes in living cells to thorough morphological analysis of tissues, spatial distribution of macromolecules in fixed or living cells, automatic collection of three-dimensional data, visualization of several labeled samples and measurement of physiological processes in living cells and tissues of organs.Aim. To determine the current possibilities and prospects of confocal laser scanning microscopy in morphological studies.Results. When analyzing scientific literature data, the opportunities and prospects of using confocal laser scanning microscopy in biomedical and morphological studies are presented. The use of the confocal diagnostic method in gynecology, molecular biology, endocrinology, endoscopy is shown. Particular attention is paid to the application of this research method in embryology. In addition, information about the role of confocal microscopy in the study of microbial pathogenesis in a three-dimensional context is provided. Data on the history, basic principles, technical innovations and advantages of confocal laser scanning microscopy are presented.Conclusion. The study of modern scientific literature has shown the importance of using confocal laser scanning microscopy in modern scientific research and diagnosis of diseases in a clinical setting, which will allow to take a new look at some aspects in modern morphology and medicine.

A comparison of the response of twoBurkholderia fungorumstrains grown as planktonic cells versus biofilm to dibenzothiophene and select polycyclic aromatic hydrocarbons

2016 ◽  
Vol 62 (10) ◽  
pp. 851-860 ◽  
Author(s):  
Nazanin Seyed Khoei ◽  
Marco Andreolli ◽  
Silvia Lampis ◽  
Giovanni Vallini ◽  
Raymond J. Turner
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Laser Scanning ◽  
Living Cells ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Planktonic Cells ◽  
Free Living ◽  
Polycyclic Aromatic ◽  
High Concentrations

In natural environments, bacteria often exist in close association with surfaces and interfaces by establishing biofilms. Here, we report on the ability of Burkholderia fungorum strains DBT1 and 95 to survive in high concentrations of hydrocarbons, and we compare their growth as a biofilm vs. planktonic cells. The 2 compounds tested were dibenzothiophene (DBT) and a mixture of naphthalene, phenanthrene, and pyrene (5:2:1) as representative compounds of thiophenes and polycyclic aromatic hydrocarbons (PAHs), respectively. The results showed that both strains were able to degrade DBT and to survive in the presence of up to a 2000 mg·L−1concentration of this compound both as a biofilm and as free-living cells. Moreover, B. fungorum DBT1 showed reduced tolerance towards the mixed PAHs (2000 mg·L−1naphthalene, 800 mg·L−1phenanthrene, and 400 mg·L−1pyrene) both as a biofilm and as free-living cells. Conversely, biofilms of B. fungorum 95 enhanced resistance against these toxic compounds compared with planktonic cells (P < 0.05). Visual observation through confocal laser scanning microscopy showed that exposure of biofilms to DBT and PAHs altered their structure: high concentrations of DBT triggered an aggregation of biofilm cells. These findings provide new perspectives on the effectiveness of using DBT-degrading bacterial strains in bioremediation of hydrocarbon-contaminated sites.

Intracellular Traffic of Glucocorticoid Receptors: Studies With Green Fluorescent Protein Chimeras in Living Cells

1997 ◽  
Vol 3 (S2) ◽  
pp. 131-132
Author(s):  
J. Barsony ◽  
J. Carroll ◽  
W. McKoy ◽  
I. Renyi ◽  
D.L. Gould ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Laser Scanning ◽  
Glucocorticoid Receptors ◽  
Target Genes ◽  
Fluorescent Protein ◽  
Living Cells ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Intracellular Traffic ◽  
Green Fluorescent

As ligand-regulated transcription factors, glucocorticoid receptors (GR) must traffic through the cytoplasm, traverse the nuclear pores, and subsequently traffic within the the nucleus to reach their target genes. Due to technical difficulties with immunocytology, little is known about the translocation process or the intranuclear localization. The recent characterization of a chromophore, green fluorescent protein (GFP), provided a general tool to fluorescently label proteins in living cells. With the development of a transcriptionally active GFP-GR chimera, it became possible to visualize GR translocation and intranuclear distribution in living cells.This chimeric receptor was transiently transfected into mouse adenocarcinoma cells, allowing the direct visualization of GR using real-time video and confocal laser scanning microscopy. Mobility of GFP-GR was analyzed with fluorescent recovery after photobleaching (FRAP).The hormone-free GFP-GR was localized in the cytoplasm figure 1). Dexamethasone (lOnM) initiated GFP-GR translocation into the nucleus (Figure 2 and 3). The translocation rate was dose- and temperature-dependent, and occurred in a pulsatile manner along cytoplasmic fibrillar structures (Figure 2). FRAP experiments showed that GFP-GR remained in motion within the nucleus after translocation.

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