scholarly journals Bioconversion of p-Tyrosol into Hydroxytyrosol under Bench-Scale Fermentation

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Zouhaier Bouallagui ◽  
Sami Sayadi
Keyword(s):  
Experimental Design ◽  
Dry Matter ◽  
Specific Productivity ◽  
Bench Scale ◽  
Olive Mill Wastewaters ◽  
Rich Solution ◽  
Olive Mill

Tyrosol hydroxylating Pseudomonas strain was previously isolated from olive mill wastewaters-irrigated soil. In the present work, experimental design was used to study the bioconversion of tyrosol in laboratory fermenters aiming at the recovery of the highest yields of hydroxytyrosol. The effects of biocatalyst loading and tyrosol concentration were studied. The bioconversion yield reached 86.9% (37.3 mM hydroxytyrosol) starting from a tyrosol concentration of 43 mM. Under these conditions, the specific productivity relative to the biocatalyst was 4.78 μM/min/g. The established model to predict bioconversion yield was validated in two bench-scale fermenters. At the downstream stage, the reaction product was recovered as a hydroxytyrosol rich solution after microfiltration and concentration under vacuum. Subsequent to this operation, hydroxytyrosol composition yielded 73.8% of the total dry matter.

Wetting and the physiological responses of grain-fed cattle in a heated environment

2004 ◽  
Vol 55 (3) ◽  
pp. 253 ◽  
Author(s):  
John B. Gaughan ◽  
M. Shane Davis ◽  
Terry L. Mader
Keyword(s):  
Heat Stress ◽  
Experimental Design ◽  
Rectal Temperature ◽  
Environmental Conditions ◽  
Dry Matter ◽  
Physiological Responses ◽  
Dry Matter Intake ◽  
Metabolic Responses ◽  
Hot Days ◽  
Carry Over

A controlled crossover experimental design was used to determine the effect of altered water sprinkling duration on heifers subjected to heat stress conditions. Heifers were subjected to 3 days of thermoneutral conditions followed by 3 days of hot conditions accompanied by water sprinkling between 1300 and 1500 h (HOT1–3). Then on the following 2 days (HOT4–5), environmental conditions remained similar, but 3 heifers were sprinkled between 1200 and 1600 h (WET) and 3 were not sprinkled (NONWET). This was followed by a 1-day period (HOT6) in which environmental conditions and sprinkling regimen were similar to HOT1–3. Rectal temperature (RT) was collected hourly, and respiration rate (RR) was monitored every 2 h on HOT Days 2, 4, 5, and 6. Dry matter intake and rate of eating were also determined. Sprinkling reduced RR and RT (P < 0.01) of all heifers during HOT1–3. During HOT4–5, WET heifers had lower (P < 0.05) RT than NONWET from 1300 to 700 h and lower RR from 1400 to 2000 h. Dry matter intake of NONWET heifers was reduced by 30.6% (P < 0.05) during HOT4–5 and by 51.2% on HOT6. On HOT4–5 the dry matter intakes of WET heifers were similar to intakes under thermoneutral conditions. During HOT6, RT was again reduced following sprinkling in all heifers. Comparison of RT and RR of NONWET and WET heifers on HOT1–3 v. HOT6 revealed that under similar environmental conditions, NONWET heifers had increased RT, partially due to carry-over from HOT4–5. However, NONWET heifers had 40% lower feed intake but tended to have lower RR on HOT6 v. HOT1–3. Only RR of WET heifers was greater on HOT6, possibly a result of switching from a 4-h back to a 2-h sprinkling period, while maintaining a 62% greater intake (5.80 v. 3.58 kg/day) than NONWET heifers during this time. Results suggest that inconsistent cooling regimens may increase the susceptibility of cattle to heat stress and elicit different physiological and metabolic responses.

Bioremediation of Olive-Mill Wastewaters By Composting

1995 ◽  
Vol 13 (6) ◽  
pp. 509-518 ◽  
Author(s):  
U. Tomati ◽  
E. Galli ◽  
L. Pasetti ◽  
E. Volterra
Keyword(s):  
Olive Mill Wastewaters ◽  
Olive Mill

scholarly journals In situ degradability of dry matter and fibrous fraction of sorghum silage

2016 ◽  
Vol 38 (2) ◽  
pp. 171 ◽  
Author(s):  
Renê Ferreira Costa ◽  
Daniel Ananias de Assis Pires ◽  
Marielly Maria Almeida Moura ◽  
José Avelino Santos Rodrigues ◽  
Vicente Ribeiro Rocha Júnior ◽  
...  
Keyword(s):  
Experimental Design ◽  
Dry Matter ◽  
Degradation Rate ◽  
Degradation Kinetics ◽  
Grain Sorghum ◽  
Dual Purpose ◽  
Ruminal Degradability ◽  
Sorghum Silage ◽  
Kinetics Of

This study aimed to evaluate in situ degradability and degradation kinetics of DM, NDF and ADF of silage, with or without tannin in the grains. Two isogenic lines of grain sorghum (CMS-XS 114 with tannin and CMS-XS 165 without tannin) and two sorghum hybrids (BR-700 dual purpose with tannin and BR-601 forage without tannin) were ensiled; dried and ground silage samples were placed in nylon bags and introduced through the fistulas. After incubation for 6, 12, 24, 48, 72 and 96 hours, bags were taken for subsequent analysis of fibrous fractions. The experimental design was completely randomized with 4 replicates and 4 treatments and means compared by Tukey’s test at 5% probability. As for the DM degradation rate, silage of CMSXS165without tannin was superior. Silages of genotypes BR700 and CMSXS 114 with tannin showed the highest values of indigestible ADF (59.54 and 43.09%). Regarding the NDF, the potential degradation of silage of CMSXS165 line without tannin was superior. Tannin can reduce ruminal degradability of the dry matter and fibrous fractions. 

scholarly journals Development of experimental structure and influence of high CO2 concentration in maize cro

2012 ◽  
Vol 32 (2) ◽  
pp. 306-314 ◽  
Author(s):  
João B. Lopes da Silva ◽  
Paulo A. Ferreira ◽  
Eduardo G. Pereira ◽  
Luís C. Costa ◽  
Glauco V. Miranda
Keyword(s):  
Experimental Design ◽  
Dry Matter ◽  
Co2 Concentration ◽  
Dry Mass ◽  
C4 Plant ◽  
Active Radiation ◽  
High Co2 ◽  
Photosynthetic Rates ◽  
High Co2 Concentration ◽  
Experimental Structure

Maize is a C4 plant that shows few or no response to high [CO2]. Thus, this study aimed to analyze the photosynthetic rate and yield of maize under high [CO2] and develop open-top chambers (OTC) to create an atmosphere enriched with CO2. The experiment was conducted between October 2008 and March 2009. The OTCs were developed in modular scheme. Measurement of photosynthetic rates, transpiration, stomata conductance, grain yield and dry matter were performed. The experimental design was randomized blocks with four replications and three treatments: P1 - plants grown in OTC with 700 ppm [CO2], P2 - plants grown in OTC with environmental [CO2], and P3 - control, cultivated in open field. The results were analyzed by ANOVA and Tukey's test (Pr< 0.05). The chambers can reduce by 25% the photosynthetically active radiation and increase the air and leaf temperatures. Plants under high [CO2] (P1) showed the highest photosynthetic rates and the lowest stomata conductance and transpiration. The total weight of grains (g) and dry mass of shoots (g) showed no increases for P1, despite their higher photosynthetic rates.

scholarly journals Growth and mineral nutrition of Solanum americanum

2010 ◽  
Vol 28 (2) ◽  
pp. 293-299 ◽  
Author(s):  
S. Bianco ◽  
L.B. Carvalho ◽  
M.S. Bianco
Keyword(s):  
Experimental Design ◽  
Nutrient Solution ◽  
Mineral Nutrition ◽  
Dry Matter ◽  
Dry Matter Production ◽  
Perennial Crops ◽  
Greenhouse Trial ◽  
Matter Production ◽  
Solanum Americanum ◽  
Experimental Stage

A greenhouse trial was carried out from November 1995 to April 1996 at FCAV/UNESP, Brazil, aiming to study the dry matter production and the accumulation and distribution of macronutrients in Solanum americanum, an important weed for annual and perennial crops in Brazil. The plants were grown in seven liter pots with sand substrate, irrigated daily with Hoagland & Arnon nutrient solution. The experimental design was completely randomized with four replicates. The treatments corresponded to evaluation times at 14 day intervals, beginning 21 days after emergence (DAE). In each evaluation, the plants of four pots were analyzed for dry matter production and macronutrient content. S. americanum had a small dry matter and macronutrient accumulation at the beginning of the experimental stage, increasing after 77 DAE and reaching the maximum theoretical value at 142, 142, 164, 149, 140, 149 and 152 DAE, for dry matter, N, P, K, Ca, Mg, and S, respectively. K and N were the most accumulated macronutrients for S. americanum plants.

scholarly journals EFFECT OF DIFFERENT DOSES OF VERMICOMPOST ON THE GROWTH OF APULEIA LEIOCARPA (VOG) MACBR. SEEDLINGS

2005 ◽  
Vol 3 (2) ◽  
pp. 11 ◽  
Author(s):  
Marcos Vinicius Winckler Caldeira ◽  
Peter Spathelf ◽  
Leonir Rodrigues Barichello ◽  
Hamilton Luiz Munari Vogel ◽  
Mauro Valdir Schumacher
Keyword(s):  
Experimental Design ◽  
Biomass Production ◽  
Dry Matter ◽  
Growth Response ◽  
Diameter Growth ◽  
Seedling Production ◽  
Seedling Germination ◽  
Different Doses

The objective of this study was to determine the best vermicompost dose for seedling production of Apuleia leiocarpa. The experiment consisted of five treatments, which were conducted in a greenhouse. The following doses of vermicompost were tested: control (without vermicompost); 10% vermicompost; 20% vermicompost; 30% vermicompost and 40% vermicompost of the total container volume (185 cm3). The experimental design was entirely randomised. The substratum used was bark of Pinus sp., which was triturated and composted. A hundred days after seedling germination, the following variables were analysed: height and diameter growth, above ground dry matter, root dry matter and total dry matter. The 30% vermicompost doses showed to be optimum in growth response and biomass production of Apuleia leiocarpa seedlings.

scholarly journals Dormancy breaking in seeds of different accessions of Luffa cylindrica Roemer

2019 ◽  
Vol 9 (4) ◽  
pp. 596-602
Author(s):  
Francisca Sonally Oliveira ◽  
Lindomar Maria da Silveira ◽  
Salvador Barros Torres ◽  
Ferdinando Monteiro Carvalho Dias da Costa ◽  
Maclayver Christiano Caetano Nunes ◽  
...  
Keyword(s):  
Experimental Design ◽  
Central America ◽  
Dry Matter ◽  
Commercial Production ◽  
Expanded Polystyrene ◽  
Index Number ◽  
Experimental Plot ◽  
Dormancy Breaking ◽  
Luffa Cylindrica ◽  
Emergence Percentage

Luffa cylindrica Roemer is abundant in countries of Asia, Central America and South America. Dormancy in luffa seeds is caused by the impermeability of the coat, which may be a major obstacle in the commercial production of seedlings for the cultivation of this species. Thus, this study aimed to evaluate different methods to break dormancy in different accessions of luffa. The experimental design was completely randomized, in a 6 (accessions) x 5 (dormancy breaking treatments) factorial arrangement, with four replicates. Seeds were planted on expanded polystyrene trays, containing commercial substrate Plantmax®. The analyzed variables were: emergence percentage; emergence speed index; number of true leaves; plant height; stem diameter; root length; shoot fresh and dry matter, and root dry matter. The first two variables were analyzed in the entire experimental plot and the others only in the evaluated area of the plot. Scarification with sandpaper nº 80 and cut with pruning pliers on the opposite end to the hilum were the methods of dormancy breaking which led to the best results. Due to the variability of the results among the accessions, it was not possible to recommend one method of dormancy breaking for luffa seeds that is generalized for all of them.

Olive-mill wastewaters: a promising substrate for microbial lipase production

2006 ◽  
Vol 97 (15) ◽  
pp. 1828-1833 ◽  
Author(s):  
Alessandro D’Annibale ◽  
Giovanni Giovannozzi Sermanni ◽  
Federico Federici ◽  
Maurizio Petruccioli
Keyword(s):  
Lipase Production ◽  
Microbial Lipase ◽  
Olive Mill Wastewaters ◽  
Olive Mill
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