Saksenaea dorisiae sp. nov., a New Opportunistic Pathogenic Fungus from Europe

A new species, Saksenaea dorisiae (Mucoromycotina, Mucorales), isolated from a water sample originating from a private well in Manastirica, Petrovac, in the Republic of Serbia (Europe), is described and illustrated. The new taxon is well supported by multilocus phylogenetic analysis that included the internal transcribed spacer (ITS) region, domains D1 and D2 of the 28S rRNA gene (LSU), and translation elongation factor-1α gene (tef-1α), and it is resolved in a clade with S. oblongispora and S. trapezispora. This fungus is characterized by its moderately slow growth at 15 and 37°C, sparse rhizoids, conical-shaped sporangia, and short-cylindrical sporangiospores. Saksenaea dorisiae is a member of the opportunistic pathogenic genus often involved in severe human and animal mucormycoses encountered in tropical and subtropical regions. Despite its sensitivity to several conventional antifungals (terbinafine and ciclopirox), the fungus can potentially evoke clinically challenging infections. This is the first novel taxon of the genus Saksenaea described from the moderately continental climate area of Europe.

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A New European Species of The Opportunistic Pathogenic GenusSaksenaea–S. dorisiaeSp. Nov.

10.1101/597955 ◽

2019 ◽

Author(s):

Roman Labuda ◽

Andreas Bernreiter ◽

Doris Hochenauer ◽

Christoph Schüller ◽

Alena Kubátová ◽

...

Keyword(s):

Elongation Factor ◽

Rrna Gene ◽

28S Rrna ◽

New Taxon ◽

Internal Transcribed Spacer Region ◽

Translation Elongation ◽

Opportunistic Pathogenic ◽

A New Species ◽

Continental Climate ◽

Translation Elongation Factor 1Α

AbstractA new speciesSaksenaea dorisiae(Mucoromycotina, Mucorales), recently isolated from a water sample originating from a private well in a rural area of Serbia (Europe), is described and illustrated. The new taxon is well supported by phylogenetic analysis of the internal transcribed spacer region (ITS), domains D1 and D2 of the 28S rRNA gene (LSU), and translation elongation factor-1α gene (TEF-1α), and it is resolved in a clade withS. oblongisporaandS. trapezispora. This fungus is characteristic by its moderately slow growth at 15 and 37°C, sparse rhizoids, conical-shaped sporangia and short-cylindrical sporangiospores.S. dorisiaeis a member of the opportunistic pathogenic genus often involved in severe human and animal mucormycoses encountered in tropical and subtropical regions. Despite its sensitivity to several conventional antifungals (terbinafine and ciclopirox), the fungus is potentially causing clinically challenging infections. This is the first novel taxon of the genusSaksenaeadescribed from the moderately continental climate area of Europe.

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Neptunomyces aureus gen. et sp. nov. (Didymosphaeriaceae, Pleosporales) isolated from algae in Ria de Aveiro, Portugal

MycoKeys ◽

10.3897/mycokeys.60.37931 ◽

2019 ◽

Vol 60 ◽

pp. 31-44 ◽

Cited By ~ 1

Author(s):

Micael F. M. Gonçalves ◽

Tânia F. L. Vicente ◽

Ana C. Esteves ◽

Artur Alves

Keyword(s):

Phylogenetic Analyses ◽

Elongation Factor ◽

Its Region ◽

Physiological Data ◽

New Taxon ◽

Translation Elongation ◽

Ria De Aveiro ◽

The Family ◽

Elongation Factor 1 Alpha ◽

Elongation Factor 1

A collection of fungi was isolated from macroalgae of the genera Gracilaria, Enteromorpha and Ulva in the estuary Ria de Aveiro in Portugal. These isolates were characterized through a multilocus phylogeny based on ITS region of the ribosomal DNA, beta-tubulin (tub2) and translation elongation factor 1 alpha (tef1-α) sequences, in conjunction with morphological and physiological data. These analyses showed that the isolates represented an unknown fungus for which a new genus, Neptunomycesgen. nov. and a new species, Neptunomyces aureussp. nov. are proposed. Phylogenetic analyses supported the affiliation of this new taxon to the family Didymosphaeriaceae.

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Identification and Characterization of Diaporthe ambigua, D. australafricana, D. novem, and D. rudis Causing a Postharvest Fruit Rot in Kiwifruit

Plant Disease ◽

10.1094/pdis-10-16-1535-re ◽

2017 ◽

Vol 101 (8) ◽

pp. 1402-1410 ◽

Cited By ~ 6

Author(s):

Gonzalo A. Díaz ◽

Bernardo A. Latorre ◽

Mauricio Lolas ◽

Enrique Ferrada ◽

Paulina Naranjo ◽

...

Keyword(s):

San Francisco ◽

Plant Pathogens ◽

Elongation Factor ◽

Its Region ◽

Fruit Rot ◽

Lesion Length ◽

Translation Elongation ◽

Wide Range ◽

Postharvest Rot ◽

Diaporthe Ambigua

Diaporthe spp. are important plant pathogens causing wood cankers, blight, dieback, and fruit rot in a wide range of hosts. During surveys conducted during the 2013 and 2014 seasons, a postharvest rot in Hayward kiwifruit (Actinidia deliciosa) was observed in Chile. In order to identify the species of Diaporthe associated with this fruit rot, symptomatic fruit were collected from seven kiwifruit packinghouses located between San Francisco de Mostazal and Curicó (central Chile). Twenty-four isolates of Diaporthe spp. were identified from infected fruit based on morphological and cultural characters and analyses of nucleotides sequences of three loci, including the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2), a partial sequences of the β-tubulin, and translation elongation factor 1-α genes. The Diaporthe spp. identified were Diaporthe ambigua, D. australafricana, D. novem, and D. rudis. Multilocus phylogenetic analysis revealed that Chilean isolates were grouped in separate clades with their correspondent ex-types species. All species of Diaporthe were pathogenic on wounded kiwifruit after 30 days at 0°C under normal and controlled-atmosphere (2% O2 and 5% CO2) storage and they were sensitive to benomyl, pyraclostrobin, and tebuconazole fungicides. D. ambigua isolates were the most virulent based on the lesion length measured in inoculated Hayward and Jintao kiwifruit. These findings confirm D. ambigua, D. australafricana, D. novem, and D. rudis as the causal agents of kiwifruit rot during cold storage in Chile. The specie D. actinidiae, a common of Diaporthe sp. found associated with kiwifruit rot, was not identified in the present study.

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Survey, Identification, and Characterization of Cylindrocarpon-Like Asexual Morphs in Spanish Forest Nurseries

Plant Disease ◽

10.1094/pdis-01-18-0171-re ◽

2018 ◽

Vol 102 (11) ◽

pp. 2083-2100 ◽

Cited By ~ 4

Author(s):

Beatriz Mora-Sala ◽

Ana Cabral ◽

Maela León ◽

Carlos Agustí-Brisach ◽

Josep Armengol ◽

...

Keyword(s):

Dna Sequences ◽

Elongation Factor ◽

Its Region ◽

Phylogenetic Position ◽

Translation Elongation ◽

Forest Nurseries ◽

Asexual Morphs ◽

Identification And Characterization ◽

Elongation Factor 1

Cylindrocarpon-like asexual morphs infect herbaceous and woody plants, mainly in agricultural scenarios, but also in forestry systems. The aim of the present study was to characterize a collection of Cylindrocarpon-like isolates recovered from the roots of a broad range of forest hosts from nurseries showing decline by morphological and molecular studies. Between 2009 and 2012, 17 forest nurseries in Spain were surveyed and a total of 103 Cylindrocarpon-like isolates were obtained. Isolates were identified based on DNA sequences of the partial gene regions histone H3 (his3). For the new species, the internal transcribed spacer and intervening 5.8S nrRNA gene (ITS) region, β-tubulin (tub2), and translation elongation factor 1-α (tef1) were also used to determine their phylogenetic position. Twelve species belonging to the genera Cylindrodendrum, Dactylonectria, and Ilyonectria were identified from damaged roots of 15 different host genera. The species C. alicantinum, D. macrodidyma, D. novozelandica, D. pauciseptata, D. pinicola, D. torresensis, I. capensis, I. cyclaminicola, I. liriodendri, I. pseudodestructans, I. robusta, and I. rufa were identified. In addition, two Dactylonectria species (D. hispanica sp. nov. and D. valentina sp. nov.), one Ilyonectria species (I. ilicicola sp. nov.), and one Neonectria species (N. quercicola sp. nov.) are newly described. The present study demonstrates the prevalence of this fungal group associated with seedlings of diverse hosts showing decline symptoms in forest nurseries in Spain.

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Identification of Pathogenic Fusarium spp. Responsible for Root Rot of Angelica sinensis and Characterization of their Biological Enemies in Dingxi, China

Plant Disease ◽

10.1094/pdis-06-21-1249-re ◽

2022 ◽

Author(s):

Liu Yang ◽

Tian Yuan ◽

Xia Zhao ◽

Yue Liang ◽

UWAREMWE CONSTANTINE ◽

...

Keyword(s):

Root Rot ◽

Elongation Factor ◽

Its Region ◽

Fungal Species ◽

Cortical Cells ◽

Translation Elongation ◽

Field Samples ◽

Fusarium Pathogens ◽

Rot Disease ◽

Serious Disease

Root rot is a serious disease in plantations of A. sinensis, severely affecting yield and quality and threatening sustainable production. Fusarium isolates (n=32) were obtained from field samples of root rot tissue, leaves and infected soil. Isolates were identified by comparing the sequences of their internal transcribed spacer (ITS) region and translation elongation factor 1-ɑ (TEF-1ɑ) to sequences of known species in the NCBI-database. These Fusarium isolates include F. tricinctum (43.75%), F. equiseti (31.25%), F. solani (9.37%), F. oxysporum (6.25%), F. acuminatum (6.25%), and F. incarnatum (3.12%). For pathogenicity testing under greenhouse conditions, seven isolates were selected based on a phylogenetic analysis, including four strains of F. tricinctum and one strain each of F. solani, F. oxysporum, and F. acuminatum. The seven isolates were all pathogenic but differed in their ability to infect: the four F. tricinctum strains were capable pathogens causing root rot in A. sinensis at 100% incidence and the highly aggressive. Furthermore, the symptoms of root rot induced by those seven isolates were consistent with typical root rot cases in the field, but their disease severity varied. Observed histopathological preparations of F. tricinctum-infected seedlings and tissue-slides results showed this fungal species can penetrate epidermal cells and colonize the cortical cells where it induces necrosis and severe plasmolysis. Plate confrontation experiments showed that isolated rhizosphere bacteria inhibited the Fusarium pathogens that cause root rot in A. sinensis. Our results provide timely information for informing the use of biocontrol agents for suppression of root rot disease.

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Baorangia duplicatopora (Boletaceae, Boletales), a new bolete from tropical China

Phytotaxa ◽

10.11646/phytotaxa.508.1.4 ◽

2021 ◽

Vol 508 (1) ◽

Author(s):

XU ZHANG ◽

ZHI-QUN LIANG ◽

SHUAI JIANG ◽

CHANG XU ◽

XIN-HUA FU ◽

...

Keyword(s):

New Species ◽

Dna Sequences ◽

Phylogenetic Analyses ◽

Elongation Factor ◽

Its Region ◽

Tropical Region ◽

Translation Elongation ◽

Line Drawings ◽

Microscopic Features ◽

Tropical China

Baorangia duplicatopora is described as a new species from Hainan Province, a tropical region of China. It is morphologically characterized by large to very large basidiomata with a dull rose red, rose pink to purplish red pileus, compound pores, pileus context near hymenophore and stipe context staining blue when injured, a red stipe, and cheilocystidia wider than those of other Baorangia species. Phylogenetic analyses of DNA sequences from part of the 28S gene, the nuc rDNA internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α gene (TEF1) also confirmed that B. duplicatopora forms an independent lineage within Baorangia. Detailed descriptions, color photographs of fresh basidiomata, and line drawings of microscopic features of the new species are presented. A key to species of Baorangia in the world is also provided.

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Characterization and pathogenicity of Diplodia, Lasiodiplodia and Neofusicoccum species causing Botryosphaeria canker and dieback of apple trees in central Chile

Plant Disease ◽

10.1094/pdis-06-21-1291-re ◽

2021 ◽

Author(s):

Gonzalo A. Díaz ◽

Adrián Vinicio Valdez ◽

Francois Halleen ◽

Enrique Ferrada ◽

Mauricio A. Lolas ◽

...

Keyword(s):

Disease Incidence ◽

Elongation Factor ◽

Its Region ◽

Morphological Identification ◽

Apple Trees ◽

Translation Elongation ◽

Central Chile ◽

Growing Seasons ◽

First Time ◽

Elongation Factor 1

In recent years, the number of apple trees affected by Botryosphaeria cankers and dieback has considerably increased in central Chile. This study aimed to identify the species of Botryosphaeriaceae associated with canker and dieback symptoms, estimate disease incidence and distributions, and study their pathogenicity and virulence on apple and other fruit crops. A field survey of 34 commercial orchards of apple (7-to 30-year-old) was conducted in 16 localities obtaining 270 symptomatic branches and trunks samples in 2017 and 2018 growing seasons. The incidence of Botryosphaeria canker and dieback ranged between 5 and 40%, and a total of 255 isolates of Botryosphaeriaceae spp. were obtained from 238 cankers. Morphological identification along with phylogenetics studies of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rDNA, part of the translation elongation factor 1-α (tef1-α), and part of the beta tubulin (tub2) genes allowed to identify Diplodia mutila (n = 49 isolates), D. seriata (n = 136 isolates), Lasiodiplodia theobromae (n = 16 isolates) and Neofusicoccum arbuti (n= 54 isolates). L. theobromae was isolated mainly of apple dieback from northern localities. All pathogens tested were pathogenic, causing canker and dieback symptoms on lignified twigs of apple, pear, walnut, and green grapevine shoots in the field. Isolates of N. arbuti were the most virulent by reproducing more severe cankers on lignified tissues inoculated. This study reports D. mutila and L. theobromae for the first time associated with Botryosphaeria canker and dieback in Chile, and it is the first description of N. arbuti causing apple dieback worldwide.

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Two new Lycoperdon species collected from Korea: L. albiperidium and L. subperlatum spp. nov.

Phytotaxa ◽

10.11646/phytotaxa.260.2.1 ◽

2016 ◽

Vol 260 (2) ◽

pp. 101 ◽

Cited By ~ 1

Author(s):

CHANG SUN KIM ◽

JONG WON JO ◽

YOUNG-NAM KWAG ◽

GI-HO SUNG ◽

JAE-GU HAN ◽

...

Keyword(s):

Sequence Data ◽

Elongation Factor ◽

Morphological Characteristics ◽

Its Region ◽

Its Sequences ◽

Translation Elongation ◽

Protein Coding ◽

Dna Sequence Data ◽

Coding Regions ◽

Elongation Factor 1 Alpha

Thirty-four Lycoperdon specimens from Korea were examined with the internal transcribed spacer (ITS) region of ribosomal DNA sequence data. The result of the ITS sequences phylogenetic analysis indicated that the Korean specimens represented nine different species. To confirm the taxonomic position of these species, we conducted an intensive morphological investigation, and additional phylogenetic investigation of the protein coding regions RNA polymerase subunit II (RPB2) and translation elongation factor 1-alpha (TEF1). We discovered two new species (L. albiperidium and L. subperlatum) and one (L. ericaeum) newly discovered in Korea. Lycoperdon albiperidium is closely related to L. ericaeum based on ITS, RPB2 and TEF1 sequence data, but these species were distinguishable by morphological characteristics, especially the shape of the basidiocarps, the diameter of the eucapillitial threads and the size of the basidospores. Lycoperdon subperlatum is quite similar to the European and American L. perlatum based on morphological characteristics. However, L. subperlatum is clearly distinct from European and American L. perlatum based on ITS, RPB2 and TEF1 sequence data, and somewhat differs from them in macro- and microscopic characteristics. Based on morphological characteristics, L. ericaeum is related to L. subumbrinum and L. lividum but it is distinguishable by the presence of fragile, eucapillitial threads, the diameters of the threads and ITS sequences. Here, we describe four Lycoperdon species collected in Korea.

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New cyanobacterium Nodosilinea svalbardensis sp. nov. (Prochlorotrichaceae, Synechococcales) isolated from alluvium in Mimer river valley of the Svalbard archipelago

Phytotaxa ◽

10.11646/phytotaxa.442.2.2 ◽

2020 ◽

Vol 442 (2) ◽

pp. 61-79 ◽

Cited By ~ 1

Author(s):

DENIS DAVYDOV ◽

SERGEI SHALYGIN ◽

ANNA VILNET

Keyword(s):

Phylogenetic Analyses ◽

Natural Populations ◽

Its Region ◽

Total Evidence ◽

Rrna Gene ◽

New Taxon ◽

Cyanobacterial Strain ◽

Svalbard Archipelago ◽

Molecular Approaches ◽

The 16S Rrna Gene

A cyanobacterial strain isolated from the Svalbard archipelago was studied using morphological, ecological, and molecular approaches. The morphology of natural populations fit well the description of the Leptolyngbya s.l. however, in culture, they formed specific nodules that prevented affiliation to this genus. Further phylogenetic analyses including the 16S rRNA gene and 16S-23S ITS region revealed that the strain corresponds to the genus Nodosilinea. Based on this total evidence approach, we provide here a description of the new taxon Nodosilinea svalbardensis sp. nov.

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Identification of Neofusicoccum parvum causing canker and twig blight on Ficus carica in Italy

Phytopathologia Mediterranea ◽

10.36253/phyto-10798 ◽

2020 ◽

Vol 59 (1) ◽

pp. 213-218

Author(s):

Dalia AIELLO ◽

Giorgio GUSELLA ◽

Alberto FIORENZA ◽

Vladimiro GUARNACCIA ◽

Giancarlo POLIZZI

Keyword(s):

Sequence Data ◽

Elongation Factor ◽

Its Region ◽

Ficus Carica ◽

Translation Elongation ◽

Neofusicoccum Parvum ◽

Elongation Factor 1 Alpha ◽

Twig Blight ◽

Pathogenicity Tests ◽

Fungal Colony

During June 2018, several symptomatic fig (Ficus carica) cuttings, showing twig blight, subcortical discolouration and apical dieback were collected from a nursery in Catania province, Sicily (Italy). Isolations from diseased tissue consistently showed the presence of the same fungal colony. Morphology of the fungal isolates together with sequence data of the nuclear rDNA internal transcriber spacer (ITS) region, translation elongation factor 1-alpha (tef1) gene and partial beta-tubulin (tub2) gene of representatives isolates revealed the presence of the fungus Neofusicoccum parvum. Pathogenicity tests were conducted by inoculating fig cuttings with mycelial plugs. After 10 days, the inoculated plants developed cankers similar to those observed in the greenhouse and after 26 days all inoculated plants were dead. To the best of our knowledge, this is the first report worldwide of N. parvum causing disease on this host.

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