scholarly journals Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Aleksandra Pieczykolan ◽  
Wioleta Pietrzak ◽  
Renata Nowak ◽  
Józefina Pielczyk ◽  
Karolina Łamacz
Keyword(s):  
Solvent Extraction ◽  
High Efficiency ◽  
Wide Spectrum ◽  
Extraction Methods ◽  
Accelerated Solvent Extraction ◽  
Extraction Techniques ◽  
Ultrasound Assisted Extraction ◽  
Considerable Saving ◽  
Esi Ms ◽  
Assisted Extraction

Tiliroside exhibits a wide spectrum of effects on the human body; considering expensive synthesis of tiliroside, linden trees seem to be a good source of this compound. For the first time, 46 various extraction methods were developed to receive tiliroside from Tilia L., including ultrasound-assisted extraction, maceration, maceration with stirring, accelerated solvent extraction, and extraction under reflux. The effects of extraction techniques, solvents, additives, and temperature on the content of tiliroside were studied using analytical and statistical methods. A new, rapid, simple, sensitive, and selective liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed to determine the content of tiliroside in Tilia L. flowers. The LC-ESI-MS/MS analysis demonstrated the highest content of tiliroside in Tilia L. flowers obtained using accelerated solvent extraction (ASE) where 70% ethanol with addition of 1% acetic acid was used as a solvent (7.400 ± 0.019 mg of tiliroside per g dry extract).The results showed that the extracts of Tiliae inflorescentia contained large amounts of tiliroside; therefore, they are good sources of this compound. Moreover, ASE was found to be superior to other extraction techniques due to its high efficiency as well as considerable saving of time and solvent.

scholarly journals Accelerated Solvent Extraction as a Green Tool for the Recovery of Polyphenols and Pigments from Wild Nettle Leaves

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 803
Author(s):  
Maja Repajić ◽  
Ena Cegledi ◽  
Valentina Kruk ◽  
Sandra Pedisić ◽  
Fırat Çınar ◽  
...  
Keyword(s):  
Solvent Extraction ◽  
Antioxidant Capacity ◽  
Accelerated Solvent Extraction ◽  
Ultrasound Assisted Extraction ◽  
Extraction Solvent ◽  
Green Approach ◽  
Assisted Extraction ◽  
Chlorophylls A And B ◽  
Different Temperatures ◽  
Ultrasound Assisted

This study aimed to investigate the performance of accelerated solvent extraction (ASE) as a green approach for the recovery of polyphenols and pigments from wild nettle leaves (NL). ASE was operated at different temperatures (20, 50, 80 and 110 °C), static times (5 and 10 min) and cycle numbers (1–4) using ethanol (96%) as an extraction solvent. In order to compare the efficiency of ASE, ultrasound assisted extraction (UAE) at 80 °C for 30 min was performed as a referent. Polyphenol and pigment analyses were carried out by HPLC and antioxidant capacity was assessed by ORAC. Seven polyphenols from subclasses of hydroxycinnamic acids and flavonoids, along with chlorophylls a and b and their derivatives and six carotenoids and their derivatives were identified and quantified. Chlorogenic acid was the most abundant polyphenol and chlorophyll a represented the dominant pigment. ASE conditions at 110 °C/10 min/3 or 4 cycles proved to be the optimal for achieving the highest yields of analyzed compounds. In comparison with UAE, ASE showed better performance in terms of yields and antioxidants recovery, hence delivering extract with 60% higher antioxidant capacity. Finally, the potential of NL as a functional ingredient from natural sources can be successfully accessed by ASE.

Comparison of Conventional and Ultrasound-Assisted Extraction Techniques for Extraction of Phenolic Compounds from Coconut Husk

2019 ◽  
Vol 891 ◽  
pp. 83-89
Author(s):  
Attapon Nitiwattananon ◽  
Saipin Thanachasai
Keyword(s):  
Solvent Extraction ◽  
Phenolic Compounds ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Agitation Speed ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Conventional Solvent Extraction ◽  
Ultrasound Assisted ◽  
Conventional Extraction

In this study, ultrasound-assisted extraction (UAE) was compared with conventional extraction methods, including conventional solvent extraction without agitation (CSE), conventional solvent extraction with agitation at 50 rpm (CSE50) and 150 rpm (CSE150), for the extraction of phenolic compounds from coconut (Cocos nucifera L.) husk. The extraction yield, total phenolic content (TPC) and total flavonoid content (TFC) were examined. The antioxidant capacity of C. nucifera extracts was determined by using 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Experimental results showed that UAE gave the highest extraction yield, TPC, TFC and antioxidant capacities (ABTS and DPPH), followed by CSE150, CSE50 and CSE, respectively. UAE was found to be more effective than conventional extraction methods. Conventional solvent extraction with higher agitation speed exhibited higher extraction efficiency than those with lower agitation speed and without agitation.

scholarly journals Extraction Methods for the Isolation of Isoflavonoids from Plant Material

2017 ◽  
Vol 15 (1) ◽  
pp. 34-45 ◽  
Author(s):  
Tomasz Blicharski ◽  
Anna Oniszczuk
Keyword(s):  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Phytochemical Analysis ◽  
Extraction Techniques ◽  
Ultrasound Assisted Extraction ◽  
Pharmacological Activities ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Biological And Pharmacological Activities

AbstractThe purpose of this review is to describe and compare selected traditional and modern extraction methods employed in the isolation of isoflavonoids from plants. Conventional methods such as maceration, percolation, or Soxhlet extraction are still frequently used in phytochemical analysis. Despite their flexibility, traditional extraction techniques have significant drawbacks, including the need for a significant investment of time, energy, and starting material, and a requirement for large amounts of potentially toxic solvents. Moreover, these techniques are difficult to automate, produce considerable amount of waste and pose a risk of degradation of thermolabile compounds. Modern extraction methods, such as: ultrasound-assisted extraction, microwave-assisted extraction, accelerated solvent extraction, supercritical fluid extraction, and negative pressure cavitation extraction, can be regarded as remedies for the aforementioned problems. This manuscript discusses the use of the most relevant extraction techniques in the process of isolation of isoflavonoids, secondary metabolites that have been found to have a plethora of biological and pharmacological activities.

scholarly journals Accelerated Solvent Extraction of Antioxidant Compounds from Gardeniae Fructus and Its Acetylcholinesterase Inhibitory and PC12 Cell Protective Activities

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2805
Author(s):  
Yiling Fan ◽  
Xueying Li ◽  
Lan Ding ◽  
Weiying Zhou ◽  
Guangzi Xu ◽  
...  
Keyword(s):  
Solvent Extraction ◽  
Pc12 Cells ◽  
Antioxidant Activities ◽  
Compositional Analysis ◽  
Accelerated Solvent Extraction ◽  
Neuroprotective Activity ◽  
Antioxidant Compounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Medicinal Food

Gardeniae fructus is a common neuroprotective medicinal food in China, however the extraction efficiency and mixture activities are rarely mentioned. In this study, accelerated solvent extraction (ASE) parameters were optimized by a response surface methodology to extract antioxidants from Gardeniae fructus. Neuroprotective activity was evaluated using H2O2 and amyloid-β25–35 peptide-treated PC12 cells. By comparing with three other extract methods (i.e., heated refluxing extraction (HRE), ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE)), it was found that the yield (35.10%), total iridoids (27.69%), total flavonoid (6.12%) content, antioxidant activities (IC50 on DPPH, 164.46 µg/mL; FRAP value 4703.54 μmol/L), and acetylcholinesterase inhibitory ability (IC50 92.58 µg/mL) of ASE extract under the optimal condition (150 °C temperature, 10 min static time, 60% ethanol, 2 extract cycles) were significantly higher than other extract methods. The strongest ability to protect PC12 cells from damage was also present in ASE extract, as evidenced by decreasing lactate dehydrogenase and malondialdehyde levels, elevating superoxide dismutase and glutathioneperoxidase activities. Compositional analysis indicated that the extremely high crocetin level in ASE extract (1.30 μg/mg) may offer great potential. Our results indicated that ASE is a proper extraction method that could offer great potential for finding the neuroprotective ability of Gardeniae fructus for the treatment of AD.

scholarly journals Extraction of Phenolic Compounds and Terpenes from Cannabis sativa L. By-Products: From Conventional to Intensified Processes

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 942
Author(s):  
Emilie Isidore ◽  
Hamza Karim ◽  
Irina Ioannou
Keyword(s):  
Phenolic Compounds ◽  
Cannabis Sativa ◽  
Biological Activities ◽  
Operating Conditions ◽  
Extraction Techniques ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Whole Plant ◽  
Conventional Solvent Extraction ◽  
By Products

Cannabis sativa L. is a controversial crop due to its high tetrahydrocannabinol content varieties; however, the hemp varieties get an increased interest. This paper describes (i) the main categories of phenolic compounds (flavonoids, stilbenoids and lignans) and terpenes (monoterpenes and sesquiterpenes) from C. sativa by-products and their biological activities and (ii) the main extraction techniques for their recovery. It includes not only common techniques such as conventional solvent extraction, and hydrodistillation, but also intensification and emerging techniques such as ultrasound-assisted extraction or supercritical CO2 extraction. The effect of the operating conditions on the yield and composition of these categories of phenolic compounds and terpenes was discussed. A thorough investigation of innovative extraction techniques is indeed crucial for the extraction of phenolic compounds and terpenes from cannabis toward a sustainable industrial valorization of the whole plant.

scholarly journals Extraction of Peppermint Essential Oils and Lipophilic Compounds: Assessment of Process Kinetics and Environmental Impacts with Multiple Techniques

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2879
Author(s):  
Aleksandar Radivojac ◽  
Oskar Bera ◽  
Zoran Zeković ◽  
Nemanja Teslić ◽  
Živan Mrkonjić ◽  
...  
Keyword(s):  
Co2 Emissions ◽  
Electricity Consumption ◽  
Well Being ◽  
Extraction Methods ◽  
Ultrasound Assisted Extraction ◽  
Statistical Parameters ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
The Many ◽  
Product Sales

Consumers are becoming more mindful of their well-being. Increasing awareness of the many beneficial properties of peppermint essential oil (EO) has significantly increased product sales in recent years. Hydrodistillation (HD), a proven conventional method, and a possible alternative in the form of microwave-assisted hydrodistillation (MWHD) have been used to isolate peppermint EO. Standard Soxhlet and alternatively supercritical fluid (SFE), microwave-assisted, and ultrasound-assisted extraction separated the lipid extracts. The distillations employed various power settings, and the EO yield varied from 0.15 to 0.80%. The estimated environmental impact in terms of electricity consumption and CO2 emissions suggested that MWHD is an energy efficient way to reduce CO2 emissions. Different extraction methods and solvent properties affected the lipid extract yield, which ranged from 2.55 to 5.36%. According to the corresponding values of statistical parameters, empiric mathematical models were successfully applied to model the kinetics of MWHD and SFE processes.

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