AURKA rs8173 G>C Polymorphism Decreases Wilms Tumor Risk in Chinese Children

Wilms tumor is the most common type of renal malignancy in children. Previous studies have demonstrated that single nucleotide polymorphisms (SNPs) in the AURKA gene could predispose to several human malignancies. We recruited 145 cases and 531 cancer-free controls to investigate whether AURKA gene variants modify Wilms tumor susceptibility. Three AURKA SNPs (rs1047972 C>T, rs2273535 T>A, and rs8173 G>C) were genotyped by the Taqman methodology. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of association between AURKA SNPs and Wilms tumor risk. We found that only the rs8173 G>C polymorphism was significantly associated with Wilms tumor risk (GC vs. GG: adjusted OR (AOR) = 0.50, 95% CI = 0.35–0.73, P=0.0002; GC/CC vs. GG: AOR = 0.60, 95% CI = 0.42–0.88, P=0.008). Stratification analysis revealed that rs8173 GC/CC genotypes were associated with Wilms tumor risk among children aged >18 months (AOR = 0.56, 95% CI = 0.34–0.93, P=0.024), male children (AOR = 0.54, 95% CI = 0.33–0.90, P=0.017), and children with clinical stage III + IV diseases (AOR = 0.56, 95% CI = 0.35–0.90, P=0.017). Haplotype analysis indicated that the CAG haplotype was significantly associated with increased Wilms tumor risk. In conclusion, our findings indicated that the AURKA rs8173 G>C polymorphism was associated with decreased Wilms tumor risk in Chinese children.

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Association between PHOX2B gene rs28647582 T>C polymorphism and Wilms tumor susceptibility

Bioscience Reports ◽

10.1042/bsr20192529 ◽

2019 ◽

Vol 39 (10) ◽

Cited By ~ 1

Author(s):

Ao Lin ◽

Wen Fu ◽

Wenwen Wang ◽

Jinhong Zhu ◽

Jiabin Liu ◽

...

Keyword(s):

Cellular Proliferation ◽

Wilms Tumor ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Phox2b Gene ◽

Tumor Susceptibility ◽

Pediatric Solid Tumors ◽

Tumor Risk ◽

Proliferation And Differentiation ◽

Stratified Analysis

Abstract Wilms tumor is one of the most common pediatric solid tumors. The pair-like homeobox 2b (PHOX2B) gene is an important transcription factor that regulates cellular proliferation and differentiation in early life. The association between PHOX2B single nucleotide polymorphisms (SNPs) and Wilms tumor risk has not been investigated. Therefore, we conducted a case-control study involving 145 Wilms tumor patients and 531 controls to explore the association between the PHOX2B rs28647582 T>C polymorphism and Wilms tumor susceptibility. The association between the PHOX2B rs28647582 T>C polymorphism and Wilms tumor susceptibility was assessed by odds ratios (ORs) and 95% confidence intervals (CIs). Our results indicated that PHOX2B rs28647582 T>C polymorphism did not significantly alter Wilms tumor susceptibility. However, in the stratified analysis, we found that TC/CC genotypes significantly increased Wilms tumor risk among children older than 18 months (adjusted OR = 1.77, 95% CI = 1.07–2.95, P=0.027) and those with clinical stages III+IV (adjusted OR = 1.75, 95% CI = 1.09–2.82, P=0.022), when compared with those with TT genotype. Our study suggested that PHOX2B rs28647582 T>C was weakly associated with Wilms tumor susceptibility. Our conclusions need further validation with a larger sample size.

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H19 gene polymorphisms and Wilms tumor risk in Chinese children: a four-center case-control study

10.21203/rs.2.23509/v1 ◽

2020 ◽

Author(s):

Wenya Li ◽

Rui-Xi Hua ◽

Mi Wang ◽

Da Zhang ◽

Jinhong Zhu ◽

...

Keyword(s):

Wilms Tumor ◽

Clinical Stage ◽

Genetic Variations ◽

Nucleotide Polymorphisms ◽

Predisposing Factor ◽

Single Nucleotide ◽

Tumor Risk ◽

H19 Gene ◽

Stratified Analysis ◽

Control Study

Abstract Background: Wilms tumor is the most common pediatric renal cancer. However, genetic bases behind Wilms tumor remain largely unknown. H19 is a critical maternally imprinted gene. Previous studies indicated that Single nucleotide polymorphisms (SNPs) in the H19 can modify the risk of several human malignancies. Epigenetic errors at the H19 locus lead to biallele silencing in Wilms tumors. Genetic variations in the H19 may be related to Wilms tumor susceptibility.Methods: We conducted a four-center study to investigate whether H19 SNPs was a predisposing factor to Wilms tumor. Three polymorphisms in the H19 (rs2839698 G>A, rs3024270 C>G, rs217727 G>A) were genotyped in 355 cases and 1070 cancer-free controls,using Taqman method. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to evaluate the strength of the associations.Results: We found that all of these three polymorphisms were significantly associated with Wilms tumor risk alterations. Carriers of 1, 2 and 1-2 risk genotypes were inclined to develop Wilms tumor compared with those without risk genotype [adjusted odds ratio (OR)=1.36, 95% confidence interval (CI)=1.02-1.80, P =0.037; adjusted OR=1.84, 95% CI=1.27-2.67, P =0.001; adjusted OR=1.50, 95% CI=1.17-1.92, P =0.002, respectively]. The stratified analysis further revealed that rs2839698 AA, rs217727 AA, and 1-2 risk genotypes could strongly increase Wilms tumor risk among children above 18 months of age, males, and with clinical stage I+II disease.Conclusion: Our findings indicate that genetic variations in the H19 may confer Wilms tumor risk.

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TP53 rs1042522 C>G polymorphism and Wilms tumor susceptibility in Chinese children: a four-center case–control study

Bioscience Reports ◽

10.1042/bsr20181891 ◽

2019 ◽

Vol 39 (1) ◽

Cited By ~ 5

Author(s):

Peng Liu ◽

Zhenjian Zhuo ◽

Wenya Li ◽

Jiwen Cheng ◽

Haixia Zhou ◽

...

Keyword(s):

Significant Relationship ◽

Wilms Tumor ◽

Tp53 Gene ◽

Chinese Children ◽

Logistic Regression Models ◽

Tumor Susceptibility ◽

Tumor Risk ◽

Larger Sample ◽

Control Study

Abstract Wilms tumor is the most common renal malignancy that occurs in children. TP53 gene is considered as a tumor-suppressing gene through controlling cell growth. TP53 gene rs1042522 C>G (Arg72Pro) polymorphism is widely investigated in various types of cancers. However, it is not established if TP53 rs1042522 C>G polymorphism is a candidate variant for Wilms tumor risk. The aim of the study was to determine whether TP53 rs1042522 C>G polymorphism is responsible for the risk of Wilms tumor in Chinese children. All subjects (355 cases and 1070 controls) from four centers of China were genotyped for rs1042522 C>G polymorphism. The effect of rs1042522 C>G polymorphism on Wilms tumor prevalence was analyzed using logistic regression models. We failed to detect a significant relationship between rs1042522 C>G polymorphism and Wilms tumor risk. Further stratification analysis also could not detect a significant relationship. We conclude that TP53 rs1042522 C>G polymorphism might not have enough impact on the risk of Wilms tumor. More validation study with larger sample size will be required to better define the role of TP53 rs1042522 C>G polymorphism in Wilms tumor risk.

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Association Between LIN28A Gene Polymorphisms and Glioma Susceptibility in Chinese Children

Cancer Control ◽

10.1177/10732748211040009 ◽

2021 ◽

Vol 28 ◽

pp. 107327482110400

Author(s):

Huiqin Guo ◽

Yuxiang Liao ◽

Ao Lin ◽

Huiran Lin ◽

Xiaokai Huang ◽

...

Keyword(s):

Gene Polymorphisms ◽

Case Control Study ◽

Malignant Tumors ◽

Clinical Stage ◽

Case Control ◽

Chinese Children ◽

Stage Iii ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Control Study

Gliomas are the most prevalent brain tumors among children and adolescents. The occurrence and development of various malignant tumors is closely related with LIN28A gene, but its relationship with glioma susceptibility has not been widely discovered. In this case-control study, we conducted four single nucleotide polymorphisms (SNPs) (rs3811464 G>A, rs3811463 T>C, rs34787247 G>A, and rs11247957 G>A) of LIN28A gene to investigate whether they increase the risk of glioma. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to evaluate their relationship. There was no significant correlation between four SNPs and glioma risk in single polymorphism and conjoint analysis. However, in stratification analysis, we found that rs3811463 TC/CC may add to the risk of glioma with clinical stage III (adjusted OR = 3.16, 95% CI = 1.15-8.70, P = .026) or stage III+IV patients (adjusted OR = 2.05, 95% CI = 1.02-4.13, P = .044). Our research suggested that four SNPs of LIN28A gene have a weak relationship with the risk of glioma in Chinese children. LIN28A rs3811463 TC/CC may increase the possibility of glioma in clinical stage III or stage III+IV patients which need larger samples and further confirmation.

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miR-618 rs2682818 C>A polymorphism decreases Hirschsprung disease risk in Chinese children

Bioscience Reports ◽

10.1042/bsr20193989 ◽

2020 ◽

Vol 40 (5) ◽

Author(s):

Yi Zheng ◽

Tongyi Lu ◽

Xiaoli Xie ◽

Qiuming He ◽

Lifeng Lu ◽

...

Keyword(s):

Disease Risk ◽

Malignant Tumors ◽

Hirschsprung Disease ◽

Chinese Children ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Functional Region ◽

Tumor Susceptibility ◽

Southern Chinese ◽

Stratified Analysis

Abstract MicroRNAs (miRNAs) are endogenous non-coding small RNAs that play an important role in the development of many malignant tumors. In addition, recent studies have reported that single nucleotide polymorphisms (SNPs) located in the miRNA functional region was inextricably linked to tumor susceptibility. In the present study, we investigated the susceptibility between miR-618 rs2682818 C>A and Hirschsprung disease (HSCR) in the Southern Chinese population (1470 patients and 1473 controls). Odds ratios (ORs) and 95% confidence intervals (CIs) were used for estimating the strength of interrelation between them. We found that the CA/AA genotypes of miR-618 rs2682818 were associated with a decreased risk of HSCR when compared with the CC genotype (OR = 0.84, 95% CI = 0.72–0.99, P=0.032). Based on the stratified analysis of HSCR subtypes, the rs2682818 CA/AA genotypes were able to significantly lessen the risk of HSCR compared with CC genotype in patients with long-segment HSCR (adjusted OR = 0.70, 95% CI = 0.52–0.93, P=0.013). In conclusion, our results indicated that the miR-618 rs2682818 C>A polymorphism was associated with a reduced risk of HSCR in Chinese children, especially in patients with long-segment HSCR (L-HSCR) subtype.

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H19 gene polymorphisms and Wilms tumor risk in Chinese children: a four-center case-control study

10.21203/rs.2.23509/v2 ◽

2020 ◽

Author(s):

Wenya Li ◽

Rui-Xi Hua ◽

Mi Wang ◽

Da Zhang ◽

Jinhong Zhu ◽

...

Keyword(s):

Case Control Study ◽

Wilms Tumor ◽

Genetic Variations ◽

Nucleotide Polymorphisms ◽

Predisposing Factor ◽

Tumor Susceptibility ◽

Tumor Risk ◽

Male Patients ◽

Stratified Analysis ◽

Control Study

Abstract Background Wilms tumor is the most common pediatric renal cancer. However, genetic bases behind Wilms tumor remain largely unknown. H19 is a critical maternally imprinted gene. Previous studies indicated that nucleotide polymorphisms (SNPs) in the H19 can modify the risk of several human malignancies. Epigenetic errors at the H19 locus lead to biallele silencing in Wilms tumors. Genetic variations in the H19 may be related to Wilms tumor susceptibility. Methods We conducted a four-center study to investigate whether H19 SNPs was a predisposing factor to Wilms tumor. Three polymorphisms in the H19 (rs2839698 G>A, rs3024270 C>G, rs217727 G>A) were genotyped in 355 cases and 1070 cancer-free controls, using Taqman method. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to evaluate the strength of the associations. Results We found that all of these three polymorphisms were significantly associated with Wilms tumor risk alterations. Carriers of 1, 2 and 1-2 risk genotypes were inclined to develop Wilms tumor compared with those without risk genotype (adjusted OR=1.36, 95% CI=1.02-1.80, P=0.037; adjusted OR=1.84, 95% CI=1.27-2.67, P=0.001; adjusted OR=1.50, 95% CI=1.17-1.92, P=0.002, respectively). The stratified analysis further revealed that rs2839698 AA, rs217727 AA, and 1-2 risk genotypes could strongly increase Wilms tumor risk among male patients above 18 months of age.Conclusion Our findings indicate that genetic variations in the H19 may confer Wilms tumor risk.

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The association of miR34b/c and TP53 gene polymorphisms with Wilms tumor risk in Chinese children

Bioscience Reports ◽

10.1042/bsr20194202 ◽

2020 ◽

Vol 40 (2) ◽

Author(s):

Juxiang Wang ◽

Susu Lou ◽

Xiaokai Huang ◽

Yixiao Mo ◽

Zhen Wang ◽

...

Keyword(s):

Wilms Tumor ◽

Clinical Stage ◽

Tp53 Gene ◽

Effect Analysis ◽

Tumor Susceptibility ◽

Downstream Target ◽

Functional Polymorphisms ◽

Tumor Risk ◽

Downstream Target Gene ◽

Transcription Factor P53

Abstract Wilms tumor is the most common pediatric malignancy in the kidney. The miR34b/c is a downstream target gene of the transcription factor p53. The important role of TP53 mutations, the methylation of miR34b/c, and the interaction between these two molecules in tumorigenesis have been well documented. Due to the biological connection between p53 and miR34b/c, in the present study, we investigated the association between polymorphisms in these two molecules and Wilms tumor susceptibility through genotyping two important functional polymorphisms (miR34b/c rs4938723 T>C and TP53 rs1042522 C>G) in 183 cases and 603 controls. Adjusted odds ratios (ORs) and 95% confidence intervals (CIs) derived from the logistic regression analysis were used to assess the correlation of miR34b/c rs4938723 and TP53 rs1042522 polymorphisms with Wilms tumor risk. Our results indicated that the association of miR34b/c rs4938723 and TP53 rs1042522 polymorphisms with Wilms tumor susceptibility was not statistically significant. Stratified analysis by age, gender, and clinical stage, as well as combined effect analysis were also performed, yet, no significant association was found. In conclusion, our study indicated a lack of association between the two selected polymorphisms and Wilms tumor susceptibility. Our findings need to be verified in studies with larger sample size in the future.

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H19 gene polymorphisms and Wilms tumor risk in Chinese children: a four‐center case‐control study

Molecular Genetics & Genomic Medicine ◽

10.1002/mgg3.1584 ◽

2021 ◽

Author(s):

Wenya Li ◽

Rui‐Xi Hua ◽

Mi Wang ◽

Da Zhang ◽

Jinhong Zhu ◽

...

Keyword(s):

Gene Polymorphisms ◽

Case Control Study ◽

Wilms Tumor ◽

Case Control ◽

Chinese Children ◽

Tumor Risk ◽

H19 Gene ◽

Control Study

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Associations of Two Obesity-Related Single-Nucleotide Polymorphisms with Adiponectin in Chinese Children

International Journal of Endocrinology ◽

10.1155/2017/6437542 ◽

2017 ◽

Vol 2017 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Lijun Wu ◽

Liwang Gao ◽

Xiaoyuan Zhao ◽

Meixian Zhang ◽

Jianxin Wu ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Multiple Testing ◽

Association Studies ◽

Statistical Significance ◽

Additive Model ◽

Recessive Model ◽

Chinese Children ◽

Genome Wide Association Studies ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

Purpose. Genome-wide association studies have found two obesity-related single-nucleotide polymorphisms (SNPs), rs17782313 near the melanocortin-4 receptor (MC4R) gene and rs6265 near the brain-derived neurotrophic factor (BDNF) gene, but the associations of both SNPs with other obesity-related traits are not fully described, especially in children. The aim of the present study is to investigate the associations between the SNPs and adiponectin that has a regulatory role in glucose and lipid metabolism. Methods. We examined the associations of the SNPs with adiponectin in Beijing Child and Adolescent Metabolic Syndrome (BCAMS) study. A total of 3503 children participated in the study. Results. The SNP rs6265 was significantly associated with adiponectin under an additive model (P=0.02 and 0.024, resp.) after adjustment for age, gender, and BMI or obesity statuses. The SNP rs17782313 was significantly associated with low adiponectin under a recessive model. No statistical significance was found between the two SNPs and low adiponectin after correction for multiple testing. Conclusion. We demonstrate for the first time that the SNP rs17782313 near MC4R and the SNP rs6265 near BDNF are associated with adiponectin in Chinese children. These novel findings provide important evidence that adiponectin possibly mediates MC4R and BDNF involved in obesity.

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