scholarly journals Skin Microbiota in Contact Sports Athletes and Selection of Antiseptics for Professional Hygiene

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Dilyara S. Martykanova ◽  
Nailja Ch. Davletova ◽  
Ilya A. Zemlenuhin ◽  
Venera I. Volchkova ◽  
Salavat M. Mugallimov ◽  
...  
Keyword(s):  
Bacterial Colonization ◽  
16S Rrna Gene Sequencing ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Skin Microbiota ◽  
Contact Sports ◽  
Growth Inhibition Zone ◽  
Opportunistic Bacteria

Background. The aim of this study was to assess changes in skin microbiota of wrestlers during training sessions and to determine the sensitivity of hemolytic bacterial isolates to antiseptics. Methods. The main skin bacterial isolates obtained from the skin of 15 wrestlers were identified by cultivation method, with the following MALDI Biotyper and 16S rRNA gene sequencing methods. The sensitivity of hemolytic isolates to antiseptics (Veltosept-2, Cutasept F, Chlorhexidine, Miramistin, and Hydrogen Peroxide) was evaluated by measuring the size of bacterial growth inhibition zone on agar plates. Results. Opportunistic bacteria of the species Bacillus cereus, Staphylococcus aureus, S. epidermidis, and S. saprophyticus were the most commonly found species in skin microbiota of wrestlers before and after training sessions. Representatives of all these species mostly had a hemolytic activity. An alcohol-containing antiseptic Veltosept-2 showed the strongest inhibitory effect on the bacterial isolates of athletes’ skin microbiota most frequently detected in this study. Conclusions. The general increase in the bacterial colonization of wrestlers’ skin, as well as the presence of hemolytic forms of opportunistic bacteria in cutaneous microbiota, indicates dysbiotic changes and a decrease in the protective features of the host organism. Veltosept-2 application can reduce the incidence of skin infections in contact sports athletes with the highest efficiency.

scholarly journals Rhodomyrtus tomentosa Fruit Extract and Skin Microbiota: A Focus on C. acnes Phylotypes in Acne Subjects

Cosmetics ◽  
2020 ◽  
Vol 7 (3) ◽  
pp. 53 ◽  
Author(s):  
Sandie Gervason ◽  
Isabelle Metton ◽  
Elodie Gemrot ◽  
Edwige Ranouille ◽  
Gilbert Skorski ◽  
...  
Keyword(s):  
Active Ingredient ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Skin Microbiome ◽  
Skin Microbiota ◽  
Cutibacterium Acnes ◽  
Culture Independent ◽  
Rrna Gene Sequencing ◽  
Abundant Genus ◽  
Rhodomyrtus Tomentosa

Knowing that Rhodomyrtus tomentosa is known to have antibacterial effects, this study investigated the skin microbiota with a focus on Cutibacterium acnes (C. acnes) phylotypes in subjects with acne, and determined microbiota changes after 28 days of treatment with berries Rhodomyrtus tomentosa as an active ingredient (RT). Skin swabs from seventeen acne subjects were collected and the skin microbiome was analyzed using 16S rRNA gene sequencing. A culture-independent next-generation sequencing (NGS)-based SLST (single-locus sequence typing) approach was aimed at evaluating RT extract effects on C. acnes phylotype repartition. Clinical evaluations (lesion counts) were performed at baseline (D0) and after 28 days (D28) of twice-daily application of the RT active ingredient. We determined: (1) the skin microbiota at D0 was dominated by Actinobacteria followed by Firmicutes and Proteobacteria; (2) at the genus level, Cutibacterium was the most abundant genus followed by Staphylococcus and Corynebacterium; (3) C. acnes was the major species in terms of mean abundance, followed by Staphylococcus epidermidis (S. epidermidis) and Staphylococcus hominis (S. hominis); and (4) phylotype IA1 was most represented, with a predominance of SLST type A1, followed by phylotypes II, IB, IA2, IC, and III. After 28 days of RT extract treatment, phylotype repartition were modified with a decrease in abundance (approximately 4%) of phylotype IA1 and an increase in phylotype II and III. Cutibacterium granulosum (C. granulosum) abundance also decreased. Reduction of retentional and inflammatory lesions was also noted only after RT treatment; thus, RT extract acts as a microbiota-regulating agent.

scholarly journals Cultivable mushroom growth-promoting bacteria and their impact on Agaricus blazei productivity

2013 ◽  
Vol 48 (6) ◽  
pp. 636-644 ◽  
Author(s):  
Li-Sen Young ◽  
Jiunn-Nan Chu ◽  
Asif Hameed ◽  
Chiu-Chung Young
Keyword(s):  
Nitrogen Fixation ◽  
Protein Complex ◽  
Phosphate Solubilization ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Agaricus Blazei ◽  
Bacterial Isolates ◽  
Mycelium Growth ◽  
Growth Promoting ◽  
Solubilization Ability

The objective of this work was to identify growth-promoting bacteria isolated from Agaricus blazei and to evaluate their effect on mushroom mycelial growth and productivity. A total of 56 A. blazei-associated bacterial isolates were obtained from casing soil and identified by 16S rRNA gene sequencing. Bacteria were evaluated as to phosphate-solubilization ability, nitrogen-fixation capability, and secretion of cellulase. Superior isolates were tested for their to effect on A. blazei productivity, micelial growth, and on the contents of the polysaccharide-protein complex and of N, P, K, Ca, and Mg. Bacterial isolates were identified as actinobacteria (60%), firmicutes (20%), and proteobacteria (20%). Among them, ten isolates had phosphate-solubilization ability, eight showed nitrogen-fixation capability, and 12 isolates promoted A. blazei mycelium growth. Bacterial inoculation reduces time till harvest in up to 26 days, increases fresh mushroom yield up to 215%, and increases total polysaccharide-protein complex content twofold when compared to the non-inoculated control. The actinobacteria group is the predominant A. blazei-associated phylum.

scholarly journals Salix purpurea and Eleocharis obtusa Rhizospheres Harbor a Diverse Rhizospheric Bacterial Community Characterized by Hydrocarbons Degradation Potentials and Plant Growth-Promoting Properties

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1987
Author(s):  
Fahad Alotaibi ◽  
Soon-Jae Lee ◽  
Marc St-Arnaud ◽  
Mohamed Hijri
Keyword(s):  
Carbon Source ◽  
Plant Growth ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Petrochemical Plant ◽  
Bacterial Isolates ◽  
Pgp Traits ◽  
Degrading Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

Phytoremediation, a method of phytomanagement using the plant holobiont to clean up polluted soils, is particularly effective for degrading organic pollutants. However, the respective contributions of host plants and their associated microbiota within the holobiont to the efficiency of phytoremediation is poorly understood. The identification of plant-associated bacteria capable of efficiently utilizing these compounds as a carbon source while stimulating plant-growth is a keystone for phytomanagement engineering. In this study, we sampled the rhizosphere and the surrounding bulk soil of Salixpurpurea and Eleocharis obusta from the site of a former petrochemical plant in Varennes, QC, Canada. Our objectives were to: (i) isolate and identify indigenous bacteria inhabiting these biotopes; (ii) assess the ability of isolated bacteria to utilize alkanes and polycyclic aromatic hydrocarbons (PAHS) as the sole carbon source, and (iii) determine the plant growth-promoting (PGP) potential of the isolates using five key traits. A total of 438 morphologically different bacterial isolates were obtained, purified, preserved and identified through PCR and 16S rRNA gene sequencing. Identified isolates represent 62 genera. Approximately, 32% of bacterial isolates were able to utilize all five different hydrocarbons compounds. Additionally, 5% of tested isolates belonging to genera Pseudomonas, Acinetobacter, Serratia, Klebsiella, Microbacterium, Bacillus and Stenotrophomonas possessed all five of the tested PGP functional traits. This culture collection of diverse, petroleum-hydrocarbon degrading bacteria, with multiple PGP traits, represents a valuable resource for future use in environmental bio- and phyto-technology applications.

scholarly journals Assessment and Characterization of Xylanolytic Bacteria Isolated from the Gut of Microtermes Obesi for Biomass Pretreatment

2021 ◽  
Author(s):  
Iram Batool ◽  
Falak Sher Khan ◽  
Muhammad Awais ◽  
Dawood Ahmed ◽  
Sami Ullah Khan ◽  
...  
Keyword(s):  
Corn Stover ◽  
Rice Straw ◽  
16S Rrna Gene Sequencing ◽  
Major Constituent ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Cotton Stalk ◽  
Termite Gut ◽  
Chemical Pretreatments ◽  
Rrna Gene Sequencing

Abstract The aim of current research was to examine the potential for the production of hemicelluloses degrading enzymes from bacteria harbor in termite gut. The research was also focused on the conversion of lignocellulosic biomass (Corn stover, rice straw and cotton stalk) into fermentable sugars by using enzymes from the bacterial isolates. The bacterial isolates from termite gut were screened for their ability to degrade xylan that is the major constituent of hemicelluloses. Two bacterial isolates were chosen and identified by 16S rRNA gene sequencing. Both isolates TGB9 and TGB10 belong to Bacillus geneus. The isolates have shown higher xylan degrading activity at 50 oC and optimum pH was 6.0. Xylanases from isolate TGB9 and TGB10 were utilized for sccharification of agricultural substrates (stover, rice straw and cotton stalk). As a result higher contents of reducing sugars were observed from corn stover. Xylanases from isolate TGB9 produced higher yields of reducing sugar than isolate TGB10. A comparative study was also performed among chemical pretreatment and xylanases from bacterial isolates. For this purpose agricultural substrates were also treated with H2SO4 and NaOH. Xylanases produced by TGB9 and TGB10 released higher content of sugar from agricultural substrates than chemical pretreatments. So it is concluded that termite gut have bacteria that can hydrolyze hemicelluloses more efficiently than chemicals.

scholarly journals Comparative molecular studies of halophilic bacteria from saline water and soil in the Saudi environment

2020 ◽  
Vol 36 (3) ◽  
Author(s):  
Mohamed Ahmed ◽  
Md. Mohibul Alam Khan ◽  
Saleh M. S. Al-Garni ◽  
Roop Singh Bora ◽  
Saleh A. Kabli
Keyword(s):  
Red Sea ◽  
Saline Water ◽  
Halophilic Bacteria ◽  
16S Rrna Gene Sequencing ◽  
Industrial Applications ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
High Concentration ◽  
Biochemical Tests ◽  
Water And Soil Samples

Halophilic bacteria are a microorganism that grows optimally in the presence of the very high concentration of sodium chloride. Halophiles are vital sources of various enzymes including hydrolases, which are very stable and catalytically highly efficient at high salt concentration and other extreme conditions such as high temperature, pH and presence of organic solvents.  Several hydrolases such as amylases, proteases, and lipases have been obtained from halophilic bacteria and are commonly used for various industrial applications. We initiated a screening project to isolate and characterize the halophilic bacteria from the Red Sea, which is one of the saltiest bodies of water in the world. Water and soil samples, collected from the Red Sea coast, Jeddah, Saudi Arabia, were screened for isolation of halophilic bacteria. Ten bacterial isolates were obtained, which were characterized by biochemical tests and 16S rRNA gene sequencing. Hydrolase producing bacteria among the isolates were screened by plate assay on starch and gelatin agar plates for amylase and protease, respectively.  Two bacterial isolates i.e Bacillus haynesii and Enterobacter cloacae subsp. were found to possess significant amylase and protease activity. Further characterization of both the strains is in progress.

scholarly journals Breeding Strategy Shapes the Composition of Bacterial Communities in Female Nile Tilapia Reared in a Recirculating Aquaculture System

2021 ◽  
Vol 12 ◽  
Author(s):  
Yousri Abdelhafiz ◽  
Jorge M. O. Fernandes ◽  
Simone Larger ◽  
Davide Albanese ◽  
Claudio Donati ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Nile Tilapia ◽  
16S Rrna Gene Sequencing ◽  
Study Groups ◽  
Breeding Strategy ◽  
Rrna Gene ◽  
Beneficial Bacteria ◽  
Core Microbiome ◽  
Recirculating Aquaculture ◽  
Opportunistic Bacteria

In industrial animal production, breeding strategies are essential to produce offspring of better quality and vitality. It is also known that host microbiome has a bearing on its health. Here, we report for the first time the influence of crossbreeding strategy, inbreeding or outbreeding, on the buccal and intestinal bacterial communities in female Nile tilapia (Oreochromis niloticus). Crossbreeding was performed within a family and between different fish families to obtain the inbred and outbred study groups, respectively. The genetic relationship and structure analysis revealed significant genetic differentiation between the inbred and outbred groups. We also employed a 16S rRNA gene sequencing technique to understand the significant differences between the diversities of the bacterial communities of the inbred and outbred groups. The core microbiota composition in the mouth and the intestine was not affected by the crossbreeding strategy but their abundance varied between the two groups. Furthermore, opportunistic bacteria were abundant in the buccal cavity and intestine of the outbred group, whereas beneficial bacteria were abundant in the intestine of the inbred group. The present study indicates that crossbreeding can influence the abundance of beneficial bacteria, core microbiome and the inter-individual variation in the microbiome.

scholarly journals Profiling the microbial community of a Triassic halite deposit in Northern Ireland: an environment with significant potential for biodiscovery

2019 ◽  
Vol 366 (22) ◽  
Author(s):  
Julianne Megaw ◽  
Stephen A Kelly ◽  
Thomas P Thompson ◽  
Timofey Skvortsov ◽  
Brendan F Gilmore
Keyword(s):  
Northern Ireland ◽  
16S Rrna Gene Sequencing ◽  
Antimicrobial Activities ◽  
Chemical Diversity ◽  
Hypersaline Environment ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Culture Independent ◽  
Rrna Gene Sequencing ◽  
County Antrim

ABSTRACT Kilroot salt mine, a Triassic halite deposit located in County Antrim, Northern Ireland, is the only permanent hypersaline environment on the island of Ireland. In this study, the microbiome of this unstudied environment was profiled for the first time using conventional and enhanced culturing techniques, and culture independent metagenomic approaches. Using both conventional isolation plates and iChip devices, 89 halophilic archaeal isolates from six known genera, and 55 halophilic or halotolerant bacterial isolates from 18 genera were obtained, based on 16S rRNA gene sequencing. The archaeal isolates were similar to those previously isolated from other ancient halite deposits, and as expected, numerous genera were identified in the metagenome which were not represented among the culturable isolates. Preliminary screening of a selection of isolates from this environment identified antimicrobial activities against a panel of clinically important bacterial pathogens from 15 of the bacterial isolates and one of the archaea. This, alongside previous studies reporting the discovery of novel biocatalysts from the Kilroot mine microbiome, suggests that this environment may be a new, untapped source of of chemical diversity with high biodiscovery potential.

Selection of Bacillus thuringiensis against Pathogenic Nematodes Attacking Pepper Tree

2020 ◽  
Vol 36 (3) ◽  
pp. 57-62
Author(s):  
Minh Vo Van ◽  
Tuan Vo Chau ◽  
My Pham Тhi ◽  
Ha Do Thu ◽  
Trang Le Vu Khanh
Keyword(s):  
Bacillus Thuringiensis ◽  
16S Rrna Gene Sequencing ◽  
Inhibitory Effect ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Nematode Eggs ◽  
Science And Education ◽  
Pepper Tree ◽  
Rrna Gene Sequencing ◽  
Meloidogyne Sp

Nine strains belonging to the Bacillus genus have been isolated from soil samples in Tien Phuoc district, Quang Nam province. They were capable of surviving at 42°C, and the Bl, B4, B7 and B9 strains could produce toxic crystals at this temperature. B7 had the most promising characteristics in terms of spore-forming ability. The result of the 16S rRNA gene sequencing showed that B7 belongs to the Bacillus thuringiensis species, which is known to effectively control root-knot Meloidogyne sp. nematodes attacking pepper tree. This study was aimed at evaluating the inhibitory effect of the bacterial isolate under study on root-knot eggs and juveniles. It was shown that the highest inhibitory activity of the cultures of the bacterial strains under study was observed at their concentration of 109cells/mL; in this case, up to 89.67% of nematode eggs and 100% of juveniles J2 were killed after 10 h of treatment. Bacillus thuringiensis, Meloidogyne sp., pepper tree, root-knot, nematodes We are grateful to the Danang University of Science and Education and Duy Tan University for supporting this study.

Chitinolytic bacteria in the intestinal tract of Japanese coastal fishes

2006 ◽  
Vol 52 (12) ◽  
pp. 1158-1163 ◽  
Author(s):  
Shiro Itoi ◽  
Toshihiro Okamura ◽  
Yuki Koyama ◽  
Haruo Sugita
Keyword(s):  
16S Rrna ◽  
Intestinal Tract ◽  
Intestinal Bacteria ◽  
16S Rrna Gene Sequencing ◽  
Colloidal Chitin ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Coastal Fish ◽  
Chitinolytic Bacteria ◽  
Rrna Gene Sequencing

Intestinal bacteria from several coastal fish species were screened on 1/20 PYBG medium containing 0.2% colloidal chitin, and 361 bacteria capable of decomposing colloidal chitin were isolated. These isolates were subsequently screened on media containing either 0.5% α-chitin or 0.5% β-chitin resulting in the identification of 31 α-chitinolytic and 275 β-chitinolytic bacterial isolates. Partial 16S rRNA gene sequencing was carried out and homology searches of the resultant sequences against the DDBJ, EMBL, and GenBank databases revealed that the majority (99%) of the chitinolytic bacteria isolated belonged to the Vibrionaceae. Phylogenetic analysis using a Bayesian approach showed that the α-chitinolytic bacteria belonging to the Vibrionaceae formed a separate cluster from the non-α-chitinolytic bacteria in the Vibrionaceae.Key words: chitinolytic bacteria, 16S rRNA, α-chitin, coastal fish, intestinal bacteria.

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