scholarly journals Multiplex Tests for Respiratory Tract Infections: The Direct Utility of the FilmArray Respiratory Panel in Emergency Department

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Siyuan Yang ◽  
Hui Li ◽  
Yunxia Tang ◽  
Fengting Yu ◽  
Chengjie Ma ◽  
...  
Keyword(s):  
Emergency Department ◽  
Respiratory Tract ◽  
Influenza A ◽  
Respiratory Tract Infections ◽  
Pathogen Detection ◽  
Point Of Care ◽  
Respiratory Pathogens ◽  
Point Of Care Testing ◽  
Tract Infections ◽  
First Time

Background. The FilmArray Respiratory Panel with multiplex targets for respiratory pathogens has been widely used and verified in clinical trials in special test settings. However, it is necessary to evaluate the panel’s performance at the point of care directly, in nonspecific test settings. Methods. Patients with respiratory tract infections were enrolled from among emergency department visitors, and all steps, including the collection of specimens and testing, were performed by our clinicians. Results. Among 270 patients, 196 (72.6%) patients were found to have one or more pathogens. For signal pathogen detection, influenza A virus had the highest rate of detection; 45 (16.7%) of the patients had two or more respiratory pathogens codetected, and most of the multiplex pathogens were rhinovirus/enterovirus codetected with Bordetella pertussis (17.8%). The information provided by the FilmArray had an impact on the prescription of antimicrobials, and there were differences in the rates of antibiotic prescriptions and anti-influenza prescriptions among patients. Conclusions. Use of the FilmArray by clinical staff was successfully implemented in the emergency department for the first time in China. The FilmArray has the potential for point-of-care testing in nonspecific settings.

scholarly journals Multiplex tests for respiratory tract infections, direct utility of FilmArray respiratory panel in the emergency department

2020 ◽  
Author(s):  
Siyuan Yang ◽  
Hui Li ◽  
Yunxia Tang ◽  
Fengting Yu ◽  
Chengjie Ma ◽  
...  
Keyword(s):  
Emergency Department ◽  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Point Of Care ◽  
Winter Season ◽  
Diagnostic Methods ◽  
Respiratory Pathogens ◽  
Atypical Pathogens ◽  
B Virus ◽  
Tract Infections

Abstract BackgroundThe FilmArray respiratory panel with 20 targets for viral and atypical pathogens aimed at point of care tests, as multiplex tests for respiratory tract infections it has been widely used and verified in various clinical trials. The FilmArray respiratory panel (FilmArray RP) seems to be a reliable and rapid tool in special tests settings such as clinical laboratory, and it’s necessary to evaluate the panel’s performance in point of care directly with non-specific tests settings. MethodsIn this single-centre, open, observational study, we enrolled outpatients with respiratory tract infection from emergency department during 2016-2017 winter season, specimens from participants were detected using FilmArray RP. All steps including recruitment, collection of specimens and testing were performed by our clinical staff. The results of pathogens detected, clinical and demographic data were collected and analyzed. ResultsBetween October 2016 and May 2017, we enrolled 271 eligible patients, FilmArray RP was successful used in 270 patients: 196 (72.6 %) patients had been detected with one or more pathogens, 74 (27.4) patients got negative results; in the analysis of signal pathogen detected, influenza A virus had the highest rate of detection, and the rate in group ≤ 16 years, 16-49 years and ≥ 50 years was 22.8 %, 32.4 %, 31.1 % respectively; 45 (16.7 %) patients were co-detected with two or more respiratory pathogens, most multiplex pathogens were rhinovirus/enterovirus co-detected with Bordetella. pertussis (17.8 %). The outcomes provided by FilmArray RP had impact on the prescriptions of antimicrobials, there were difference in the rates of antibiotic prescriptions and anti-influenza prescriptions among patients with Flu A/B virus detected, non-Flu A/B virus pathogen detected and non-pathogen.ConclusionsThe performance of FilmArray RP using by clinical staffs was successfully implemented in emergency department for which is the first time in China. The multiplex diagnostic system significantly increased the rate of respiratory pathogens detected in diagnostic methods limited hospital. Despite this, FilmArray RP has a potential use as a tool for point of care testing in non-specific setting and operated by clinical staffs not in a laboratory.

scholarly journals C-reactive protein point-of-care testing for lower respiratory tract infections: a qualitative evaluation of experiences by GPs

2009 ◽  
Vol 27 (2) ◽  
pp. 212-218 ◽  
Author(s):  
J. W L Cals ◽  
F. H F Chappin ◽  
R. M Hopstaken ◽  
M. E van Leeuwen ◽  
K. Hood ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Lower Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Point Of Care ◽  
Qualitative Evaluation ◽  
Point Of Care Testing ◽  
C Reactive Protein ◽  
Lower Respiratory Tract Infections ◽  
Reactive Protein ◽  
Tract Infections

scholarly journals Respiratory tract infections (RTIs) in primary care: narrative review of C reactive protein (CRP) point-of-care testing (POCT) and antibacterial use in patients who present with symptoms of RTI

2020 ◽  
Vol 7 (1) ◽  
pp. e000624
Author(s):  
Jonathan Cooke ◽  
Carl Llor ◽  
Rogier Hopstaken ◽  
Matthew Dryden ◽  
Christopher Butler
Keyword(s):  
Primary Care ◽  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Point Of Care ◽  
Narrative Review ◽  
Economic Evaluations ◽  
Point Of Care Testing ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Tract Infections

Antimicrobial resistance (AMR) continues to be a global problem and continues to be addressed through national strategies to improve diagnostics, develop new antimicrobials and promote antimicrobial stewardship. Patients who attend general (ambulatory) practice with symptoms of respiratory tract infections (RTIs) are invariably assessed by some sort of clinical decision rule (CDR). However, CDRs rely on a cluster of non-specific clinical observations. A narrative review of the literature was undertaken to ascertain the value of C reactive protein (CRP) point-of-care testing (POCT) to guide antibacterial prescribing in adult patients presenting to general practitioner (GP) practices with symptoms of RTI. Studies that were included were Cochrane reviews, systematic reviews, randomised controlled trials, cluster randomised trials, controlled before and after studies, cohort studies and economic evaluations. An overwhelming number of studies demonstrated that the use of CRP tests in patients presenting with RTI symptoms reduces index antibacterial prescribing. GPs and patients report a good acceptability for a CRP POCT and economic evaluations show cost-effectiveness of CRP POCT over existing RTI management in primary care. POCTs increase diagnostic precision for GPs in the better management of patients with RTI. With the rapid development of artificial intelligence, patients will expect greater precision in diagnosing and managing their illnesses. Adopting systems that markedly reduce antibiotic consumption is a no-brainer for governments that are struggling to address the rise in AMR.

scholarly journals Rapid and Point-of-Care Testing in Respiratory Tract Infections: An Antibiotic Guardian?

2020 ◽  
Vol 3 (3) ◽  
pp. 401-417 ◽  
Author(s):  
Zaneeta Dhesi ◽  
Virve I. Enne ◽  
Justin O‘Grady ◽  
Vanya Gant ◽  
David M. Livermore
Keyword(s):  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Point Of Care ◽  
Point Of Care Testing ◽  
Tract Infections

Molecular epidemiology of rhinoviruses in Cyprus over three consecutive seasons

2014 ◽  
Vol 143 (9) ◽  
pp. 1876-1883 ◽  
Author(s):  
J. RICHTER ◽  
E. NIKOLAOU ◽  
C. PANAYIOTOU ◽  
C. TRYFONOS ◽  
M. KOLIOU ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Molecular Epidemiology ◽  
Respiratory Tract Infections ◽  
Respiratory Pathogens ◽  
Global Circulation ◽  
History Of ◽  
Tract Infections ◽  
And Control ◽  
First Time ◽  
Viral Respiratory

SUMMARYHuman rhinoviruses (HRVs) are widespread respiratory pathogens and a major cause of acute respiratory tract infections. The aim of this study was to investigate the molecular epidemiology of rhinovirus infections in children in Cyprus over three consecutive winter seasons. From a total of 116 rhinovirus-positive samples, 68 were sequenced in the 5’-UTR and VP4/VP2 regions. Thirty-six (52·9%) samples were identified as HRV-A and 27 (39·7%) as HRV-C, with only five (7·4%) samples belonging to the HRV-B species. Of these, a total of 46 different genotypes were identified. In the VP2/VP4 phylogenetic tree all strains clustered in three different well-defined clades, whereas the 5’-UTR tree exhibited clades with a mixed clustering of HRV-A and HRV-C strains reflecting the evolutionary history of recombination between HRV-A and HRV-C that has been observed previously. In summary, a high intra- and inter-season diversity of HRV types was observed. Despite its geographical isolation the frequency of HRV species in Cyprus is comparable to that reported in other regions of the world supporting the concept of an unrestricted global circulation. This study assesses, for the first time, the epidemiology of rhinovirus infections in Cypriot children and will be helpful to clinicians and researchers interested in the treatment and control of viral respiratory tract infections.

scholarly journals Current problems associated with the microbiological point-of-care testing of respiratory tract infections in primary care

2016 ◽  
Vol 11 (5) ◽  
pp. 607-610 ◽  
Author(s):  
Wendy E Kaman ◽  
Gijs Elshout ◽  
Patrick JE Bindels ◽  
Konstantinos Mitsakakis ◽  
John P Hays
Keyword(s):  
Primary Care ◽  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Point Of Care ◽  
Point Of Care Testing ◽  
Tract Infections

scholarly journals FilmArray Respiratory Panel Integrated in a field Point Of Care dispositive for the diagnosis of respiratory tract infections in rural areas in Senegal

2018 ◽  
Author(s):  
Hubert Bassene ◽  
Sophie Edouard ◽  
Georges Diatta ◽  
Jean Christophe Lagier ◽  
Oleg Mediannikov ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Rural Areas ◽  
Respiratory Tract Infections ◽  
Point Of Care ◽  
Influenza B Virus ◽  
Influenza B ◽  
Respiratory Pathogens ◽  
Field Point ◽  
Nasal Swabs ◽  
Tract Infections

The development of molecular syndrome-based kits for the diagnosis of respiratory infections offers rapid and sensitive detection of common respiratory pathogens and will have a significant impact on the care of patients. In this study, we present the results obtained after the introduction of the FilmArray respiratory panel in a field Point of care (POC) for the diagnosis of virus and bacteria responsible of respiratory tract infections in Senegal rural area. From February to August 2017, we collected nasal swabs from febrile patients that presented symptoms of respiratory tract infections in three health posts located in Niakhar. Specimens were tested on site by multiplex Polymerase Chain Reaction (PCR), using the FilmArray respiratory panel® that targets 20 pathogens, including 17 virus and 3 bacteria (bioMérieux). Nasal swabs were collected from 113 patients. The median age was 4 years (ranging from 4 months to 60 years) and 51 (45%) were males. The prevalence of respiratory pathogens was 37.5% (12/32) during the dry season and 54.3% (44/81) in the rainy season (p=0.16). The prevalence of respiratory pathogen carriage was higher in children under 5 years of age (38/55, 69.1%). The most prevalent micro-organisms detected were influenza B virus (16/113, 14%), human rhinovirus/enterovirus (10/113, 9%), parainfluenzae virus (9/113, 8%), respiratory syncytial virus (8/113, 7%), adenovirus (5/113, 4%), human metapneumovirus (3/113, 3%), Chlamydia pneumoniae (2/113, 2%) and Coronavirus (2/113, 2%). The study has demonstrated that the integration of the FilmArray respiratory panel into a field POC could significantly improve the management of respiratory tract infections in rural areas.

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