scholarly journals Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Qingdong Ma ◽  
Xiaoxiang Chen ◽  
Ke Zhang ◽  
Dahong Yao ◽  
Lu Yang ◽  
...  
Keyword(s):  
Quality Control ◽  
Morphological Characteristics ◽  
Chromatographic Fingerprint ◽  
Saussurea Involucrata ◽  
The Public ◽  
Chemical Profiling ◽  
Integrated Method ◽  
Chemical Profiles ◽  
Related Plant ◽  
Dehydrocostus Lactone

The quality control of Saussurea involucrata has been greatly improved by macroscopic and microscopic identification and chemical profiling described in Chinese Pharmacopoeia since 2005. However, these methods have their own limitations, e.g., their dependence on personal experience and expertise, and it is a huge challenge to identify closely related species that share similar or identical morphological characteristics and chemical profiles. A novel and generally accepted identification strategy is urgently needed as a complement to regulations for protecting the public health interests. In this work, a comprehensive chromatographic fingerprint method was developed and tested on 43 samples from four haplotypes of S. involucrata according to DNA barcoding. Three common patterns consisting of 20, 14, and 7 common peaks were generated by frequency filters of median, upper quartile, and 100%, respectively. Based on two formerly screened patterns, S. involucrata can be effectively identified from its five easily confused snow lotus species, including the most closely related plant (S. orgaadayi) in the orthogonal partial least-squares discriminant analysis (OPLS-DA) models. The model is supported by good R and Q coefficients. In addition, different haplotypes of S. involucrata can be discriminated in the OPLS-DA model using the 20 common peaks. Among them, peaks 9, 11, 16 (zaluzanin C), and 18 (dehydrocostus lactone) have been identified as fingerprint markers of S. involucrata via S-plots and VIP values (>1). Additionally, peaks 19 and 20 were identified as linolenic acid and linoleic acid with anti-inflammatory activity, and they were isolated from the herb for the first time. Collectively, the chromatographic fingerprint of S. involucrata can be an effective and integrated method for the identification of authentic herbs from adulterant species or related plants, and discrimination of its different haplotypes provides an objective and reliable tool for quality control.

Recommended Policies for Uses of Human Tissue in Research, Education, and Quality Control

1999 ◽  
Vol 123 (4) ◽  
pp. 296-300 ◽  
Author(s):  
William Grizzle ◽  
Wayne W. Grody ◽  
Walter W. Noll ◽  
Mark E. Sobel ◽  
Sanford A. Stass ◽  
...  
Keyword(s):  
Quality Control ◽  
Human Tissue ◽  
Medical Knowledge ◽  
Best Interests ◽  
Direct Patient Care ◽  
Research Education ◽  
The Public ◽  
Medical Specialists ◽  
The Individual ◽  
Knowledge Interests

Abstract As recipients of tissue and medical specimens, pathologists and other medical specialists regard themselves as stewards of patient tissues and consider it their duty to protect the best interests of both the individual patient and the public. The stewardship of slides, blocks, and other materials includes providing, under appropriate circumstances, patient materials for research, education, and quality control. The decision to provide human tissue for such purposes should be based on the specific (ie, direct patient care) and general (ie, furthering medical knowledge) interests of the patient and of society. The same standards of responsibility should apply to all medical professionals who receive and use specimens. This document proposes specific recommendations whereby both interests can be fostered safely, ethically, and reasonably.

scholarly journals The Attorney General, Politics, and the Public Interest. Contributions to an Evolving Constitutional Convention

2022 ◽  
pp. 1-20
Author(s):  
Michael Murphy
Keyword(s):  
Quality Control ◽  
Public Interest ◽  
Prime Minister ◽  
Attorney General ◽  
Criminal Prosecution ◽  
Prosecutorial Discretion ◽  
Constitutional Convention ◽  
The Public ◽  
Normative Framework

Abstract The prosecutorial independence of the Attorney General (AG) is a firmly established constitutional convention in Canada, but it is also an evolving convention, subject to ongoing contestation and debate. This article is a contribution to that debate. It defends a normative constitutional framework wherein the AG’s authority to make final decisions in matters of criminal prosecution is balanced against a corresponding duty to consult with cabinet and the prime minister on the public interest implications of prosecutorial decisions when the circumstances warrant. Within this normative framework, respectful contestation and debate amongst ministers, the prime minister, and the AG in determining the public interest merits of prosecution is welcomed, even encouraged, and if conducted with the requisite integrity, objectivity, and transparency, it is regarded not as a threat but as a valuable check and balance on AG independence and an indispensable form of quality control on the exercise of prosecutorial discretion.

scholarly journals Selecting a Green, Agile and Industry 4.0 Supplier With the Fuzzy-Swara-Bwm Integrated Method

2021 ◽  
Author(s):  
Mehmet Ali Tas ◽  
Serap Akcan
Keyword(s):  
Supply Chains ◽  
Industry 4.0 ◽  
Supplier Selection ◽  
Three Dimensions ◽  
Market Structures ◽  
The Public ◽  
Integrated Method ◽  
Decision Making Processes ◽  
Technological Opportunities

Abstract Businesses establish supply chains in order to continue their activities. Choosing the suppliers to take part in these supply chains poses many challenges in rapidly changing conditions. Environmental concerns in the public, competitive market structures, and developing technological opportunities affect the decision-making processes. Different criteria are taken into consideration instead of traditional criteria such as cost and service. In this study, green, agile, and Industry 4.0 dimensions and the criteria under these dimensions are defined. According to these, the problem of selecting the supplier that responds to the expectations of the markets and enables them to increase their competitiveness was discussed. Fuzzy SWARA and fuzzy BMW methods were applied in an integrated way to solve the supplier selection problem under these three dimensions. A real case study was also presented. In the study, the results obtained by creating different scenarios were compared and sensitivity analysis was made. The results obtained show that the hybrid method developed in this study is effective in supplier selection problems. As a result of the study, the most important evaluation dimension is "agile" and the most important criterion is "delivery speed".

scholarly journals Study morphology of loquat plants (Eriobotrya Japonica Lindl.) from Karo, Dairi, and Simalungun Districts, North Sumatra

2021 ◽  
Vol 912 (1) ◽  
pp. 012103
Author(s):  
Elimasni ◽  
R A Nasution
Keyword(s):  
Binary Data ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Seed Morphology ◽  
Flowering Plant ◽  
Morphological Data ◽  
Eriobotrya Japonica ◽  
Morphological Observation ◽  
Limited Information ◽  
The Public

Abstract Abstrak. Loquat (Eriobotrya japonica Lindl.) is a flowering plant that belongs to the Rosacea family. The loquat has many health benefits. Cultivation and information about loquat plants in Indonesia are still limited, so they are rarely found and known by the public. Limited information and data regarding loquat plants is also an obstacle to the development of loquat plants. Research on loquat plants aims to analyze the morphological characters in three districts, namely, Karo, Dairi, and Simalungun districts. This research was conducted using a descriptive method. The analysis of the morphological characteristics of loquat plants using morphological data scoring into binary data. The similarity between individuals was analyzed using clusters with the NTSYS program version 2.0 with the UPGMA method of the SimQual function. Morphological Observation Results Loquat plants (Eriobotrya japonica Lindl.) in Karo, Dairi, and Simalungun Districts have uniform characters in the morphology of stems, leaves, and flowers. However, the observed fruit and seed morphology showed different characters. Different characters exist in the shape of the fruit and seeds. The morphological similarity level of loquat plants was grouped at a similarity coefficient value of 95%. Clusters I and II have the highest similarity with a coefficient value of 100%. Cluster III has the lowest similarity with a coefficient value of 97%.

Hiring Auditors with a Part II Quality Control Report: An Opportunity for Increased Earnings Management or Lower Audit Fees?

2020 ◽  
Vol 5 (1) ◽  
pp. 73-93
Author(s):  
Jared Eutsler ◽  
D. Kip Holderness ◽  
Megan M. Jones
Keyword(s):  
Quality Control ◽  
Audit Quality ◽  
Audit Fees ◽  
Data Availability ◽  
Fiscal Year ◽  
The Public ◽  
Audit Firms ◽  
Public Company ◽  
General Demand ◽  
Systemic Quality

ABSTRACT The Public Company Accounting Oversight Board's (PCAOB) Part II inspection reports, which disclose systemic quality control issues that auditors fail to remediate, signal poor audit quality for triennially inspected audit firms. Auditors that receive a Part II inspection report typically experience a decrease in clients, which demonstrates a general demand for audit quality. However, some companies hire auditors that receive Part II inspection reports. We examine potential reasons for hiring these audit firms. We find that relative to companies that switch to auditors without Part II reports, companies that switch to auditors with Part II reports have higher discretionary accruals in the first fiscal year after the switch, which indicates lower audit quality and a heightened risk for future fraud. We find no difference in audit fees. Our results suggest that PCAOB Part II inspection reports may signal low-quality auditors to companies that desire low-quality audits. Data Availability: Data are available from the public sources cited in the text.

scholarly journals Chemical Profiling of Constituents of Smilacis glabrae Using Ultra-high Pressure Liquid Chromatography Coupled with LTQ Orbitrap Mass Spectrometry

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700
Author(s):  
Xiong Li ◽  
Yu Feng Zhang ◽  
Liu Yang ◽  
Yi Feng ◽  
Yuan Hui Deng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Retention Time ◽  
Chemical Profiling ◽  
Ultra High Pressure ◽  
Orbitrap Mass Spectrometry ◽  
Phenylpropanoid Glycosides ◽  
Phenolic Acid Derivatives

A reliable LC-MS method has been applied for the separation and identification of major constituents of the rhizome of Smilacis glabrae. Identification of the constituents was carried out by interpretation of their retention time, and MS and MS/MS data, especially by comparing these with Sarcandra glabra under the same LC-MS conditions, as well as the data provided by the literature. Thirty-three compounds, including catechin derivatives, flavanonols, phenolic acid derivatives and phenylpropanoid glycosides were either identified or tentatively characterized. Among them, compound 12 was deduced to be a new phenylpropanoid-substituted catechin. Fragmentation behaviors of the three major categories of compounds were also investigated. This UPLC-PDA/ESI-MSnmethod was effective for the separation and identification of the constituents and could be the basis for the comprehensive quality control of Smilacis glabrae.

scholarly journals Recent Development of Quality Control Methods for Herbal Derived Drug Preparations

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Gunawan Indrayanto
Keyword(s):  
Quality Control ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Complex Nature ◽  
Chemical Marker ◽  
Chemical Profiling ◽  
Chemical Content ◽  
Pharmaceutical Industries ◽  
Microbial Toxins

Pharmaceutical industries should apply rigorous QC (quality control) to ensure the consistency, safety, and efficacy of their herbal derived drug-preparations. QC must be performed at every stage of the production line i.e. incoming raw materials, extractions, in-process control, finished products and keeping samples. Due to the complex nature of the chemical content of herbal drugs, two approaches to QC should be taken, that is quantitative determination of the selected marker(s) compound(s), and metabolite profiling. Contamination of herbal medicines by heavy metals, pesticides, toxic metabolites, microbial toxins, pathogenic microorganisms and other foreign matter should also be evaluated. A combination of chemical profiling and multivariate analysis (MVA) is recommended as the QC tool for the botanical identification method (BIM) of herbs, extracts, herb materials, and herbal drug preparations. Microscopic methods, DNA profiling or chemical marker(s) are not recommended for use as the sole BIM due to the lack of specificity. Only markers that meet certain criteria i.e. quality active (QA) markers can be utilized as a QC tool. The limit specification range of markers used as QC tools should be described in the analytical target profile (ATP). To gain reliable results of any analysis that has been performed at any QC laboratory, the analysis method must be validated according to the newest guidance. Sample detection limit of any toxic compound(s) should be lower than its cut-off value and MPL. The reliability of any results of analysis of a QC laboratory must be evaluated by using QC-samples for each series of measurements.

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