Simultaneous Determination of Six Chromones in Saposhnikoviae Radix via Quantitative Analysis of Multicomponents by Single Marker

A method, quantitative analysis of multicomponents by single marker (QAMS), was established and fully verified based on high-performance liquid chromatography (HPLC) for simultaneous determination of six chromone indicators of Saposhnikoviae Radix (SR). In the present study, cimifugin (C), 5-O-methylvisamminol (V), hamaudol (H), and their corresponding glycosides, prim-O-glucosylcimifugin (GC), 4′-O-β-D-glucosyl-5-O-methylvisamminol (GV), and sec-O-glucosylhamaudol (GH), were selected as bioactive constituents and indicators for the quality evaluation of SR. GV was chosen as the unique reference standard, and relative correction factors (RCF) between GV and the other five chromones were calculated. The feasibility of QAMS for the analysis of chromones was investigated by comparing with the traditional external standard method (ESM). Furthermore, the method was proven to have accuracy (96.98%–102.50%), repeatability (RSD <3%), stability (RSD <3%), precision (RSD <3%), and desirable linearity (R2 ≧0.9999). Subsequently, 55 batches of commercial SR from different regions were determined by QAMS, and their contents were analyzed by principal component analysis (PCA), correlation analysis, and hierarchical cluster analysis (HCA), respectively. Based on the results, a more refined quality standard of commercial SR was proposed: SR was qualified when the total contents of six chromones were greater than 3 mg·g−1. Furthermore, SR could initially be regarded as a superior medicine when it satisfied both conditions at the same time: the total content of GC, C, GV, V, GH, and H was greater than 8 mg·g−1, and the proportion of the total content of C, V, and H was greater than 10%. This study demonstrated that the quality of SR could be successfully evaluated by the developed QAMS method; meanwhile, valuable information was provided for improving the quality standard of SR.

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Simultaneous determination of five diuretic drugs using quantitative analysis of multiple components by a single marker

BMC Chemistry ◽

10.1186/s13065-021-00764-z ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Fuchao Chen ◽

Baoxia Fang ◽

Peng Li ◽

Sicen Wang

Keyword(s):

Quantitative Analysis ◽

Simultaneous Determination ◽

Dihydrogen Phosphate ◽

Column Temperature ◽

Multiple Components ◽

External Standard Method ◽

Significant Difference ◽

Diuretic Drugs ◽

Single Marker

Abstract Background Loop diuretics are commonly used in clinical practice to manage high fluid loads and to control fluid balance. In this paper, a novel quantitative analysis method for multiple components with a single marker (QAMS) was developed for the simultaneous determination of 5 diuretic drugs furosemide, torasemide, azosemide, etacrynic acid, and bumetanide, by HPLC. Qualitative analysis was performed using relative retention time and ultraviolet (UV) spectral similarity as the double indicator. The QAMS method was conducted with etacrynic acid as an internal reference substance. The quantities of the other four diuretics were calculated by using the relative correction factors for etacrynic acid. The quantities of the 5 diuretic drugs were also determined by the external standard method (ESM). Chromatographic separation was achieved on a Shimadzu HC-C18 column (150 mm × 4.6 mm, 5 µm) using 50 mM potassium dihydrogen phosphate (pH adjusted to 4.0 with phosphoric acid) with acetonitrile (64:36, v/v) as the mobile phase at a flow rate of 1.0 mL/min and a column temperature of 30 ℃. Results Under these conditions, the 5 diuretic drugs were well separated, showing linear relationships within certain ranges. The quantitative results showed that there was no significant difference between the QAMS and ESM methods. Conclusions Overall, the HPLC-QAMS analytical scheme established in this study is a simple, efficient, economical, and accurate method for the quantitative evaluation of 5 diuretic drugs.

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Simultaneous determination of 5 diuretic drugs using an HPLC method by quantitative analysis of multiple components by a single marker

10.21203/rs.3.rs-397940/v1 ◽

2021 ◽

Author(s):

Fuchao Chen ◽

Baoxia Fang ◽

Peng Li ◽

Sicen Wang

Keyword(s):

Quantitative Analysis ◽

Simultaneous Determination ◽

Dihydrogen Phosphate ◽

Column Temperature ◽

Multiple Components ◽

External Standard Method ◽

Significant Difference ◽

Diuretic Drugs ◽

Single Marker

Abstract Background: Loop diuretics are commonly used in clinical practice to manage high fluid loads and to control fluid balance. In this paper, a novel quantitative analysis method for multiple components with a single marker (QAMS) was developed for the simultaneous determination of 5 diuretic drugs furosemide, torasemide, azosemide, etacrynic acid, and bumetanide, by HPLC. Qualitative analysis was performed using relative retention time and ultraviolet (UV) spectral similarity as the double indicator. The QAMS method was conducted with etacrynic acid as an internal reference substance. The quantities of the other four diuretics were calculated by using the relative correction factors for etacrynic acid. The quantities of the 5 diuretic drugs were also determined by the external standard method (ESM). Chromatographic separation was achieved on a Shimadzu HC-C18 column (150 mm× 4.6 mm, 5µm) using 50 mM potassium dihydrogen phosphate (pH adjusted to 4.0 with phosphoric acid) with acetonitrile (64:36, v/v) as the mobile phase at a flow rate of 1.0 mL/min and a column temperature of 30 ℃. Results: Under these conditions, the 5 diuretic drugs were well separated, showing linear relationships within certain ranges. The quantitative results showed that there was no significant difference between the QAMS and ESM methods. Conclusions: Overall, the HPLC-QAMS analytical scheme established in this study is a simple, efficient, economical, and accurate method for the quantitative evaluation of 5 diuretic drugs.

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Simultaneous determination of 5 diuretic drugs using a HPLC method by quantitative analysis of multi-components by single-marker

10.21203/rs.3.rs-230869/v1 ◽

2021 ◽

Author(s):

Fuchao Chen ◽

Baoxia Fang ◽

peng Li ◽

Sicen Wang

Keyword(s):

Quantitative Analysis ◽

Simultaneous Determination ◽

Dihydrogen Phosphate ◽

Clinical Practices ◽

Column Temperature ◽

External Standard Method ◽

Significant Difference ◽

Diuretic Drugs ◽

Single Marker

Abstract Background: Loop diuretics is commonly used in clinical practices to manage the high fluid load and to control fluid balance. In this paper, a quantitative analysis of multiple components with a single marker (QAMS) method was firstly developed for simultaneous determination of 5 diuretic drugs including furosemide, torasemide, azosemide, etacrynic acid, and bumetanide by HPLC. The qualitative analysis was performed using relative capacity factor and ultraviolet (UV) spectral similarity as the double indicator. The QAMS method was conducted with etacrynic acid as an internal reference substance. The other four diuretics were calculated by using the relative correction factors for etacrynic acid. The contents of the 5 diuretic drugs were also determined by the external standard method (ESM). The chromatographic separation was achieved on an Shimadzu HC-C18 column (150mm× 4.6mm, 5µm) using 50 mM of potassium dihydrogen phosphate (pH adjusted to 4.0 with phosphoric acid) with acetonitrile (64:36, v/v) as mobile phase at the flow rate of 1.0 ml/min and a column temperature of 30℃. Results: Under these conditions, the 5 diuretic drugs are well separated, showing linear relationships within certain ranges. The quantitative results showed that there was no significant difference between the QAMS and ESM method. Conclusions: Overall, the HPLC-QAMS analytical scheme established in this study should be a simple, efficient, economical, and accurate method for quantitative evaluation of 5 diuretic drugs.

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Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

Molecules ◽

10.3390/molecules26020479 ◽

2021 ◽

Vol 26 (2) ◽

pp. 479

Author(s):

Shihan Wang ◽

Yuanshuai Gan ◽

Hong Kan ◽

Xinxin Mao ◽

Yongsheng Wang

Keyword(s):

Fatty Acids ◽

Quantitative Analysis ◽

Unsaturated Fatty Acids ◽

Internal Standard ◽

Active Components ◽

Reliable Determination ◽

External Standard Method ◽

Single Marker ◽

Oviductus Ranae

As one of the featured products in northeast China, Oviductus Ranae has been widely used as a nutritious food, which contains a variety of bioactive unsaturated fatty acids (UFAs). It is necessary to establish a scientific and reliable determination method of UFA contents in Oviductus Ranae. In this work, six principal UFAs in Oviductus Ranae, namely eicosapentaenoic acid (EPA), linolenic acid (ALA), docosahexaenoic acid (DHA), arachidonic acid (ARA), linoleic acid (LA) and oleic acid (OA), were identified using UPLC-MS/MS. The UFAs identified in Oviductus Ranae were further separated based on the optimized RP-HPLC conditions. Quantitative analysis of multi-components by single-marker (QAMS) method was implemented in content determination of EPA, ALA, DHA, ARA and OA, where LA was used as the internal standard. The experiments based on Taguchi design verified the robustness of the QAMS method on different HPLC instruments and chromatographic columns. The QAMS and external standard method (ESM) were used to calculate the UFA content of 15 batches of Oviductus Ranae samples from different regions. The relative error (r < 0.73%) and cosine coefficient showed that the two methods obtained similar contents, and the method validations met the requirements. The results showed that QAMS can comprehensively and effectively control the quality of UFAs in Oviductus Ranae which provides new ideas and solutions for studying the active components in Oviductus Ranae.

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Simultaneous determination of osthol, columbianadin, and isoimperatorin in Angelicae pubescentis Radix by high-performance liquid chromatography (HPLC) using a quantitative analysis of multi-components by single marker (QAMS) calibration method

Instrumentation Science & Technology ◽

10.1080/10739149.2020.1742156 ◽

2020 ◽

Vol 48 (5) ◽

pp. 550-560

Author(s):

Yanmei Feng ◽

Qian Li ◽

Lan Yang ◽

Yu Zhang ◽

Daiyu Qiu ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Quantitative Analysis ◽

Simultaneous Determination ◽

High Performance ◽

Calibration Method ◽

Single Marker

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Quantitative Analysis in Combination with Fingerprint Technology and Chemometric Analysis Applied for Evaluating Six Species of Wild Paris Using UHPLC-UV-MS

Journal of Analytical Methods in Chemistry ◽

10.1155/2016/3182796 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

Yuangui Yang ◽

Ji Zhang ◽

Hang Jin ◽

Jinyu Zhang ◽

Yuanzhong Wang

Keyword(s):

Principal Component Analysis ◽

Quantitative Analysis ◽

High Performance ◽

Yunnan Province ◽

Principal Component ◽

Total Content ◽

Similarity Analysis ◽

Fast Method ◽

Paris Polyphylla

A fast method was developed by ultra high performance liquid chromatography (UHPLC) for simultaneous determination of polyphyllin I and polyphyllin II. Chemometric analyses including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) based on UHPLC chromatography were used to evaluate 38 batches from six species of Paris. Variable importance of projection was applied to select important peaks. Meanwhile, similarity analysis of UHPLC fingerprint was used to evaluate the sample of Paris polyphylla yunnanensis (PPY) and P. axialis (PA). The results indicated that the total content of saponins in PPY and PA collected from Baoshan City of Yunnan Province above 8.07 mg/g was stronger than that from other areas of the rest of species. PLS-DA showed better performance than PCA with regard to classifying the samples. Retention time during 20–27 minutes of UHPLC was screened as significant peak for distinguishing Paris of different species and original geography. All of PPY and PA with similarity value were more than 0.80. It indicated that quantitative analysis combined with chemometric and similarity analyses could evaluate the different species of Paris effectively and comprehensively.

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Quality Evaluation of Polar and Active Components in Crude and ProcessedFructus Corniby Quantitative Analysis of Multicomponents with Single Marker

Journal of Analytical Methods in Chemistry ◽

10.1155/2016/6496840 ◽

2016 ◽

Vol 2016 ◽

pp. 1-13 ◽

Cited By ~ 10

Author(s):

Yuhong Jiang ◽

Hui Chen ◽

Liling Wang ◽

Jing Zou ◽

Xiao Zheng ◽

...

Keyword(s):

Quantitative Analysis ◽

Active Components ◽

Internal Reference ◽

External Standard Method ◽

Significant Difference ◽

External Standard ◽

Single Marker ◽

Quantitative Results ◽

Processed Products

Objective. To develop a quantitative analysis of multicomponents by single-marker (QAMS) method for the simultaneous determination of polar active components inFructus Corni.Methods. Loganin was selected as the internal reference, and the relative correction factors (RCFs) of gallic acid, 5-hydroxymethyl-2-furfural, morroniside, sweroside, cornin, 7α-O-methylmorroniside, 7β-O-methylmorroniside, 7α-O-ethylmorroniside, 7β-O-ethylmorroniside, and cornuside were established. The contents of multicomponents were then calculated based on their RCFs, respectively. Contents of the 11 components were also calculated by external standard method and compared with those of the QAMS method.Results.The contents of the 11 components in 21 crude and 10 processedFructus Corniproducts were measured. No significant difference was found in the quantitative results of the QAMS and external standard methods.Conclusion. QAMS could serve as an accurate and convenient method in determining the polar and active components inFructus Corniand its processed products.

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Simultaneous Determination of Four Ingredients in Plantago Depressa by Single Marker

Journal of Chemistry ◽

10.1155/2021/4040239 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Yang Xu ◽

Yuejie Wang ◽

Shengnan Bi ◽

Yanxue Jia ◽

Huiwei Bao

Keyword(s):

Simultaneous Determination ◽

Standard Method ◽

Protocatechuic Acid ◽

Correction Factors ◽

Active Components ◽

Relative Correction ◽

External Standard Method ◽

External Standard ◽

Single Marker

Objective. To establish a quantitative analysis of multicomponents by single marker (QAMS) method for the simultaneous determination of 4 active components such as protocatechuic acid, catechin, quercetin, and luteolin in Plantago depressa. Method. 4 active components in Plantago depressa were studied. Quercetin was used as an internal reference to establish the relative correction factors among protocatechuic acid, catechin, and luteolin and calculate the contents of each component; the results were compared with those measured by the external standard method. Results. 4 components showed a good linear relationship in their respective concentration ranges (r > 0.9995). The relative correction factors (fs/k) of protocatechuic acid, catechin, and luteolin were 1.1992, 0.8613, and 1.6069, respectively. The method had good durability. The contents of protocatechuic acid, catechin, and luteolin calculated by QAMS were not significantly different from those measured by the external standard method. Conclusion. QAMS can be used to determine the content of 4 components in Plantago depressa at the same time, and the method is simple, accurate, and can be used for quality control.

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