The Aqueous Extract of Dacryodes edulis (Burseraceae) Leaves Inhibits Cell Proliferation Induced by Estradiol on the Uterus and Vagina of Ovariectomized Female Wistar Rats

Proliferation is a cellular process strongly linked to the genesis of cancer. Natural substances with antiproliferative activities are currently potential alternatives in the treatment of cancers. Dacryodes edulis, for instance, is a medicinal plant traditionally used in the treatment of cancer. Scientific studies have reported the antioxidant activity of this plant. In addition, the presence of prostate cancer chemopreventive polyphenols was reported in D. edulis extracts. Therefore, this study was aimed to evaluate the effects of the aqueous extract of D. edulis leaves on cell proliferation induced by estradiol in ovariectomized female Wistar rats. In this regard, ovariectomized (OVX) rats were cotreated with estradiol valerate (E2V) (0.75 mg/kg) and the aqueous extract of D. edulis leaves. Control groups received either the vehicle (sham-operated animals and the OVX control), E2V (0.75 mg/kg) only, or E2V (0.75 mg/kg) and tamoxifen (10 mg/kg). Treatments were administered orally for 3 consecutive days, and animals were sacrificed thereafter. Epithelial heights of the uterus and vagina were assessed. Uterine levels of total cholesterol and estradiol were determined as well. Results showed that the aqueous extract of D. edulis leaves reversed the effects of estradiol as it reduced uterine weight ( p < 0.05 ), uterine ( p < 0.05 ), and vaginal ( p < 0.001 ) epithelium heights. This antiproliferative effect of D. edulis was associated with reduced tissue (uterine) levels of estradiol ( p < 0.001 ). These results suggest that the aqueous extract of D. edulis leaves could be a potential alternative treatment for proliferation-related diseases.

Download Full-text

Ameliorative effect of Psidium guajava (l.) leaf aqueous extract on aluminium nitrate-induced liver damage in female Wistar rats

Clinical Phytoscience ◽

10.1186/s40816-020-00198-5 ◽

2020 ◽

Vol 6 (1) ◽

Author(s):

Aanuoluwapo Adeyimika Afolabi ◽

Ebenezer Adeola Ashamu ◽

Olufemi Idowu Oluranti

Keyword(s):

Aqueous Extract ◽

Liver Damage ◽

Wistar Rats ◽

Psidium Guajava ◽

Aluminium Nitrate ◽

Leaf Aqueous Extract ◽

Female Wistar Rats ◽

Ameliorative Effect

Download Full-text

Time-Dependent Effect of Oral Morphine Consumption on the Development of Cytotrophoblast and Syncytiotrophoblast Cells of the Placental Layers during the Three Different Periods of Pregnancy in Wistar Rats

Clinical and Developmental Immunology ◽

10.1155/2013/974205 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Leila Dehghani ◽

Hedayat Sahraei ◽

Rokhsareh Meamar ◽

Masoomeh Kazemi

Keyword(s):

Wistar Rats ◽

Oral Morphine ◽

Time Dependent ◽

Morphine Consumption ◽

Male Rats ◽

Dependent Manner ◽

Control Groups ◽

Female Wistar Rats ◽

Time Dependent Effect ◽

Surface Thickness

Previous studies have shown that morphine abuse during pregnancy cancause a delay in the development of the placenta and embryo and also bring about birth defects. The present study investigates the effect of the duration of maternal morphine consumption during pregnancy, as well as the impacts of morphine abuse on the development of placental layers during the three different periods of pregnancy in Wistar rats.Materials and Methodology. Female Wistar rats have been used in the present study. Experimental groups received morphine (0.05 mg/mL of drinking water) after one night of coupling with male rats for mating. On 9th, 10th, and 14th days of pregnancy, pregnant animals were killed, and placentas were removed and fixed. The cells of the placentas layers were calculated by light microscope and MOTIC and SPSS software.Results. The maternal surface thickness of the placenta was significantly increased, whereasthe fetal surface thickness of placenta was significantly decreased with morphine consumption with a time-dependent manner in experimental groups, compared to control groups. Moreover, the number of trophoblast cells increased in both maternal and fetal surfaces of placenta with respect to the duration of morphine consumption which was overt in the experimental groups compared to the control groups.Conclusion. In general, the time-dependent effects of oral morphine consumption can inhibit the development and natural functioning of cytotrophoblast and syncytiotrophoblast cells of the placental layers.

Download Full-text

Aqueous Extract of Phyllanthus amarus Schum & Thonn Leaves Attenuated the Alterations in Fluoxetine-Induced Anti-Oestrogenic Activity in Female Wistar Rats

10.26538/tjnpr/v4i7.10 ◽

2020 ◽

Vol 4 (7) ◽

pp. 310-314

Author(s):

Quadri Nurudeen ◽

◽

Musa Yakubu ◽

Keyword(s):

Aqueous Extract ◽

Wistar Rats ◽

Phyllanthus Amarus ◽

Female Wistar Rats

Download Full-text

Effects of Ficus umbellata (Moraceae) Aqueous Extract and 7-Methoxycoumarin on Scopolamine-Induced Spatial Memory Impairment in Ovariectomized Wistar Rats

Behavioural Neurology ◽

10.1155/2018/5751864 ◽

2018 ◽

Vol 2018 ◽

pp. 1-14 ◽

Cited By ~ 2

Author(s):

Stéphane Zingue ◽

Harquin Simplice Foyet ◽

Sefirin Djiogue ◽

Yannick Ezo’o Ezo’o ◽

Hervé Hervé Ngatanko Abaïssou ◽

...

Keyword(s):

Spatial Memory ◽

Aqueous Extract ◽

Memory Impairment ◽

Wistar Rats ◽

Reference Memory ◽

Ovariectomized Rats ◽

Estradiol Valerate ◽

Memory Errors ◽

Neuroprotective Effects ◽

Before And After

The present work was undertaken to evaluate the ability of F. umbellata aqueous extract and its major component 7-methoxycoumarin (MC) to improve scopolamine-induced spatial memory impairment in ovariectomized Wistar rats. For this to be done, 10 sham-operated and 30 postmenopausal-like rats were randomly distributed in eight groups (n=5) and treated with distilled water (2 mL/250 g), estradiol valerate (1 mg/kg BW), piracetam (1.5 mg/kg BW), F. umbellata aqueous extract (50 and 200 mg/kg BW), or MC (1 mg/kg BW) for 21 consecutive days. Before and after the memory impairment with scopolamine (2 mg/kg BW), animals underwent behavioral evaluations on Y- and radial mazes. As results, age and ovariectomy did not induce significant changes in the reference memory errors. While age decreased working memory errors, ovariectomy increased it. The MC as well as F. umbellata extract significantly increased (p<0.01) the percentage of spontaneous alternation and decreased (p<0.001) working and spatial reference memory errors and anxiety parameters (rearing and grooming) in ovariectomized rats. MC significantly reduced (p<0.05) the MDA level, but resulted in an increase in GSH level in brain homogenates. These results suggest that MC is endowed with neuroprotective effects and could account for the neuroprotective effects of F. umbellata in rats.

Download Full-text

Diuretic, Kaliuretic and Anti-Natriuretic Properties of Aqueous Extract of Celosia trigyna L. (Amaranthaceae) on Wistar Rats

European Journal of Medicinal Plants ◽

10.9734/ejmp/2020/v31i1930348 ◽

2020 ◽

pp. 37-47

Author(s):

Touwindséda Aimée Sawadogo ◽

Youssoufou Ouedrogo ◽

Lazare Belemnaba ◽

Filkpièrè Léonard Da ◽

Balé Bayala

Keyword(s):

Carbonic Anhydrase ◽

Aqueous Extract ◽

Wistar Rats ◽

Negative Control ◽

Urinary Flow ◽

Diuretic Effect ◽

Electrolyte Excretion ◽

Carbonic Anhydrase Inhibition ◽

Female Wistar Rats ◽

Natriuretic Effect

Aims: The purpose of this study was to determine the diuretic and electrolyte excretion properties of the aqueous extract of Celosia trigyna L. (Amaranthaceae) on female Wistar rats. Methodology: The extraction of active principles was done by macerating aerial parts of the plant. The administration of the extract and other products was done by single-dose gavage. Measurements of urinary flow rate (UFR), natriuria, kaliuria and chloruria were taken on urine collected for twenty-four hours after each product was administered. Diuretic activity (DA) and diuretic index (DI), natriuretic and saluretic effects, and carbonic anhydrase inhibition were calculated. A NaCl solution (0.9%) was used as a negative control; furosemide and aldactone were respectively used as hypokalemic and hyperkalemic positive controls. Results: We observed a significant increase in UFR, confirmed by the values of DA and DI, obtained after the administration of extract. For electrolyte excretion, we observed an increase of the kaliuria (p ˂ 0.001) and a decrease of natriuria (p ˂ 0.001) after the extract was administered; chloruria did not significantly changed. We also found a drastic anti-natriuretic dose-dependent effect while saluretic activity and carbonic anhydrase inhibition were not clearly observable. Conclusion: These results confirm the ethnobotanical data about diuretic effect of Celosia trigyna L. extract. This diuretic effect would be supported by a specific increase in K+ excretion suggesting that the extract is possibly hypokalemic. The anti-natriuretic effect suggests that extract possess an aldosterone-like properties.

Download Full-text