scholarly journals An Enzyme-Based Biosensor for the Detection of Organophosphate Compounds Using Mutant Phosphotriesterase Immobilized onto Reduced Graphene Oxide

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Muhammad Syafiq Mohd Razib ◽  
Wahhida Latip ◽  
Jahwarhar Izuan Abdul Rashid ◽  
Victor Feizal Knight ◽  
Wan Md Zin Wan Yunus ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
High Selectivity ◽  
Immobilized Enzyme ◽  
Limit Of Detection ◽  
Electrochemical Analysis ◽  
Screen Printed Carbon Electrode ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Enzymatic Detection

Enzymatic detection of organophosphate (OP) compounds can be tailored using highly sensitive and selective enzymes in the development of biosensors. Previously, mutant (YT) phosphotriesterase (PTE) was reported to efficiently hydrolyze Sp and Rp enantiomers of phosphotriester. This study reports the use of phosphotriesterase mutant YT (YT-PTE) immobilized onto reduced graphene oxide (rGO) and fabricated onto a screen-printed carbon electrode (SPCE) for electrochemical detection of OP compounds. Immobilization of YT-PTE onto rGO was secured using N-hydroxysuccinimide (NHS) and N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) cross-linker, and the resulting immobilized enzyme was able to retain up to 90% of its activity. Electrochemical analysis of the SPCE/rGO/YT-PTE showed detection of paraoxon in a linear range of 1 mM–0.005 μM with its limit of detection as low as 0.11 μM. SPCE/rGO/YT-PTE exhibited high selectivity towards paraoxon and parathion and have good reproducibility. Furthermore, detection of paraoxon was also possible in a real water sample with only minor interferences.

Synthesis of Ag nanoparticle–carbon nanotube–reduced graphene oxide hybrids for highly sensitive non-enzymatic hydrogen peroxide detection

RSC Advances ◽  
2015 ◽  
Vol 5 (49) ◽  
pp. 39037-39041 ◽  
Author(s):  
Yong Zhang ◽  
Ziying Wang ◽  
Ye Ji ◽  
Sen Liu ◽  
Tong Zhang
Keyword(s):  
Hydrogen Peroxide ◽  
Graphene Oxide ◽  
Carbon Nanotube ◽  
Reduced Graphene Oxide ◽  
Ag Nanoparticle ◽  
Hydrogen Peroxide Detection ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Enzymatic Detection ◽  
Sensing Performance

AgNP–CNT–rGO hybrids were successfully prepared and such hybrids exhibited good sensing performance for electrochemical non-enzymatic detection of hydrogen peroxide.

Highly sensitive and wide-range nonenzymatic disposable glucose sensor based on a screen printed carbon electrode modified with reduced graphene oxide and Pd-CuO nanoparticles

2015 ◽  
Vol 182 (13-14) ◽  
pp. 2183-2192 ◽  
Author(s):  
Keerthy Dhara ◽  
Ramachandran Thiagarajan ◽  
Bipin G. Nair ◽  
Gopalakrishnan Satheesh Babu Thekkedath
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Glucose Sensor ◽  
Cuo Nanoparticles ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Wide Range ◽  
Screen Printed

scholarly journals Highly sensitive determination of non-steroidal anti-inflammatory drug nimesulide using electrochemically reduced graphene oxide nanoribbons

RSC Advances ◽  
2017 ◽  
Vol 7 (52) ◽  
pp. 33043-33051 ◽  
Author(s):  
Mani Govindasamy ◽  
Veerappan Mani ◽  
Shen-Ming Chen ◽  
Thandavarayan Maiyalagan ◽  
S. Selvaraj ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Carbon Electrode ◽  
Graphene Oxide Nanoribbons ◽  
Screen Printed Carbon Electrode ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Electrochemically Reduced Graphene Oxide ◽  
Sensitive Determination

Preparation of an electrochemically reduced graphene nanoribbon (ER-GONR) film modified screen-printed carbon electrode for the highly sensitive determination of nimesulide.

scholarly journals Highly Sensitive Electrochemical Biosensor Using Folic Acid-Modified Reduced Graphene Oxide for the Detection of Cancer Biomarker

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1272
Author(s):  
Renu Geetha Bai ◽  
Kasturi Muthoosamy ◽  
Rando Tuvikene ◽  
Huang Nay Ming ◽  
Sivakumar Manickam
Keyword(s):  
Graphene Oxide ◽  
Folic Acid ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Great Promise ◽  
Sensing Applications ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

The detection of cancer biomarkers in the early stages could prevent cancer-related deaths significantly. Nanomaterials combined with biomolecules are extensively used in drug delivery, imaging, and sensing applications by targeting the overexpressed cancer proteins such as folate receptors (FRs) to control the disease by providing earlier treatments. In this investigation, biocompatible reduced graphene oxide (rGO) nanosheets combined with folic acid (FA)-a vitamin with high bioaffinity to FRs-is utilized to develop an electrochemical sensor for cancer detection. To mimic the cancer cell environment, FR-β protein is used to evaluate the response of the rGO-FA sensor. The formation of the rGO-FA nanocomposite was confirmed through various characterization techniques. A glassy carbon (GC) electrode was then modified with the obtained rGO-FA and analyzed via differential pulse voltammetry (DPV) for its specific detection towards FRs. Using the DPV technique, the rGO-FA-modified electrode exhibited a limit of detection (LOD) of 1.69 pM, determined in a linear concentration range from 6 to 100 pM. This excellent electrochemical performance towards FRs detection could provide a significant contribution towards future cancer diagnosis. Moreover, the rGO-FA sensing platform also showed excellent specificity and reliability when tested against similar interfering biomolecules. This rGO-FA sensor offers a great promise to the future medical industry through its highly sensitive detection towards FRs in a fast, reliable, and economical way.

scholarly journals Reduced Graphene Oxide Fibre Electrodes for Drug Sensing

Proceedings ◽  
2021 ◽  
Vol 68 (1) ◽  
pp. 18
Author(s):  
Sutthima Sriprasertsuk ◽  
John R. Varcoe ◽  
Carol Crean
Keyword(s):  
Cyclic Voltammetry ◽  
Graphene Oxide ◽  
Carbon Fibre ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Linear Range ◽  
Oxidation Peak ◽  
Reduced Graphene ◽  
Fibre Sensor ◽  
Model Drug

Reduced graphene oxide (rGO) fibre electrodes and their ability to sense paracetamol (as model drug) were studied. rGO was electrodeposited onto carbon fibre by two different approaches: potentiostatic deposition and cyclic voltammetry (CV) in the presence of graphene oxide solution. Carbon fibre electrodes coated with rGO (after five CV cycles) could sense paracetamol with an oxidation peak at 0.62 V (vs. Ag/AgCl). The limit of detection of this fibre sensor was found to be 36.3 µM with a linear range of 50–500 µM of paracetamol (R2 = 0.9901).

Highly Sensitive Electrochemical Bioassay for Hg(II) Detection Based on Plasma-Polymerized Propargylamine and Three-Dimensional Reduced Graphene Oxide Nanocomposite

2016 ◽  
Vol 36 (4) ◽  
pp. 1051-1065 ◽  
Author(s):  
D. L. Peng ◽  
H. F. Ji ◽  
X. D. Dong ◽  
J. F. Tian ◽  
M. H. Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Three Dimensional ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Electrochemical Bioassay
Sign in / Sign up

Export Citation Format

Share Document