scholarly journals Schistosoma japonicum Infection in Treg-Specific USP21 Knockout Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Youxiang Zhang ◽  
De-Hui Xiong ◽  
Yangyang Li ◽  
Guina Xu ◽  
Baoxin Zhang ◽  
...  
Keyword(s):  
T Cells ◽  
Liver Fibrosis ◽  
Schistosoma Japonicum ◽  
Treg Cell ◽  
Proteolytic Enzyme ◽  
Deubiquitinating Enzyme ◽  
Ifn Gamma ◽  
Antigen Preparation ◽  
Fluorescent Microsphere ◽  
Egg Antigen

The E3 deubiquitinating enzyme ubiquitin-specific proteolytic enzyme 21 (USP21) plays vital roles in physiological activities and is required for Treg-cell-mediated immune tolerance. Using a murine model infected with Schistosoma japonicum, we observed that there were more cercariae developed into adults and more eggs deposited in the livers of the USP21fl/flFOXP3Cre (KO) mice. However, immunohistochemistry showed that the degree of egg granuloma formation and liver fibrosis was reduced. In USP21fl/flFOXP3Cre mice, levels of IFN-gamma, IL-4, anti-soluble egg antigen (SEA) IgG and anti-soluble worm antigen preparation (SWAP) IgG increased in blood, as determined using ELISAs and multiplex fluorescent microsphere immunoassays, while the levels of IL-10, lL-17A, IL-23, IL-9, and anti-SEA IgM decreased. In addition, the levels of the USP21 protein and mRNA in the liver and spleen of KO mice decreased. We further observed increased Th1 responses amplified by Tregs (regulatory T cells) and compromised Th17 responses, which alleviated the liver immunopathology. We speculated that these changes were related to polarization of Th1-like Tregs. Our results revealed the roles of USP21 in Treg-cell-mediated regulation of immune interactions between Schistosoma and its host. USP21 may have potential for regulating hepatic fibrosis in patients with schistosomiasis.

scholarly journals Recruitment of Neutrophils Mediated by Vγ2 γδ T Cells Deteriorates Liver Fibrosis Induced by Schistosoma japonicum Infection in C57BL/6 Mice

2017 ◽  
Vol 85 (8) ◽  
Author(s):  
Li Zheng ◽  
Yuan Hu ◽  
Yanjuan Wang ◽  
Xibao Huang ◽  
Yuxin Xu ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Liver Fibrosis ◽  
Schistosoma Japonicum ◽  
Γδ T Cells ◽  
T Cell Subsets ◽  
Interleukin 17 ◽  
Content Type ◽  
Ifn Γ

ABSTRACT Conventional adaptive T cell responses contribute to the pathogenesis of Schistosoma japonicum infection, leading to liver fibrosis. However, the role of gamma-delta (γδ) T cells in this disease is less clear. γδ T cells are known to secrete interleukin-17 (IL-17) in response to infection, exerting either protective or pathogenic functions. In the present study, mice infected with S. japonicum are used to characterize the role of γδ T cells. Combined with the infection of S. japonicum, an extremely significant increase in the percentage of neutrophils in the CD45+ cells was detected (from approximately 2.45% to 46.10% in blood and from 0.18% to 7.34% in spleen). Further analysis identified two different γδ T cell subsets that have different functions in the formation of granulomas in S. japonicum-infected mice. The Vγ1 T cells secrete gamma interferon (IFN-γ) only, while the Vγ2 T cells secrete both IL-17A and IFN-γ. Both subtypes lose the ability to secrete cytokine during the late stage of infection (12 weeks postinfection). When we depleted the Vγ2 T cells in infected mice, the percentage of neutrophils in blood and spleen decreased significantly, the liver fibrosis in the granulomas was reduced, and the level of IL-17A in the serum decreased (P < 0.05). These results suggest that during S. japonicum infection, Vγ2 T cells can recruit neutrophils and aggravate liver fibrosis by secreting IL-17A. This is the first report that a subset of γδ T cells plays a partial role in the pathological process of schistosome infection.

scholarly journals Comparison of Recombinant Proteins from Schistosoma japonicum for Schistosomiasis Diagnosis

2010 ◽  
Vol 17 (3) ◽  
pp. 476-480 ◽  
Author(s):  
Ya-mei Jin ◽  
Ke Lu ◽  
Wei-Fang Zhou ◽  
Zhi-Qiang Fu ◽  
Jin-Ming Liu ◽  
...  
Keyword(s):  
Schistosoma Japonicum ◽  
Recombinant Proteins ◽  
Therapeutic Efficacy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Preparation ◽  
Elisa Technique ◽  
Bovine Schistosomiasis ◽  
And Control ◽  
Egg Antigen ◽  
Animal Reservoirs

ABSTRACT The most important animal reservoirs of Schistosoma japonicum in China are bovines. Diagnosis and control of bovine schistosomiasis is critical for reducing the prevalence of the disease. We screened defined diagnostic antigens that have the potential to increase the sensitivity and specificity of serological assays and to distinguish between active and prior infections. Five recombinant proteins with the potential to be diagnostic antigens were compared to the native soluble egg antigen preparation by enzyme-linked immunosorbent assay (ELISA). We evaluated the potentials of the recombinant proteins for discriminating active from prior infections, as well as the therapeutic efficacy of the established ELISA technique.

Immune Responses in Mice after Immunization with Antigens from Different Stages of the Parasite Schistosoma mansoni

2010 ◽  
Vol 65 (3-4) ◽  
pp. 289-302 ◽  
Author(s):  
Hanaa M. Gaber ◽  
Amany S. Maghraby ◽  
Mohamed Bastawy Ahmed ◽  
Andreas Ruppel ◽  
Mahmoud M. Bahgat
Keyword(s):  
T Cells ◽  
Immune Responses ◽  
Lymph Nodes ◽  
Schistosoma Mansoni ◽  
B Lymphocytes ◽  
T And B Lymphocytes ◽  
Mesenteric Lymph Nodes ◽  
Antigen Preparation ◽  
Mesenteric Lymph ◽  
Egg Antigen

Mice responses to immunization with Schistosoma mansoni antigens were investigated. Priming with cercarial antigen preparation (CAP) induced significant (P < 0.05) IgM, IgG, IgG2a, IgG2b, and IgA increases, while booster caused a significant IgG1 increase. A soluble worm antigen preparation (SWAP) caused significant IgG elevation. Priming with soluble egg antigen (SEA) caused significant IgM and IgG2a increases, while booster induced significant IgM, IgG and IgA increases. CAP-immunized mice sera (IMS) recognized CAP peptides ranging from 23 - 78 kDa. SWAP-IMS recognized SWAP peptides ranging from 40 - 75 kDa. SEA-IMS recognized SEA peptides ranging from 33 - 101 kDa. The cross-reactive peptides among the 3 antigens were identified. CAP caused significant increases in mesenteric lymph nodes (MLNs) CD4,8+, B lymphocytes, CD8+ thymocytes, CD4+ T and B splenocytes. SWAP priming caused significant increases in MLNs CD4,8+ thymocytes and B splenocytes. SWAP booster caused significant increases in MLNs CD8+ T and B lymphocytes, CD4,8+ thymocytes and CD4+ T and B splenocytes. SEA caused significant increase in CD4+ T cells.

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