scholarly journals Microcomputed Tomography and Histological Study of Bone Regeneration Using Tooth Biomaterial with BMP-2 in Rabbit Calvarial Defects

Scanning ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Puneet Wadhwa ◽  
Jeong Hun Lee ◽  
Bing Cheng Zhao ◽  
HongXin Cai ◽  
Jae-Suk Rim ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Histological Study ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Control Group ◽  
Block Type ◽  
Graft Material ◽  
Synthetic Bone ◽  
Ct Analysis

Our study was aimed to analyze the osteoinductive effect of powdered and block type autogenous bone graft along with bone morphogenetic protein (BMP-2) as compared to synthetic bone graft. Three circular bicortical defects were made in the calvaria of each rabbit and randomly divided into three groups as follows: powdered tooth biomaterial+BMP-2, block tooth biomaterial+BMP-2, and control group: synthetic bone+BMP-2. The samples taken from these defects after 4 and 8 weeks were analyzed histologically along with micro CT analysis. In our study, both powered and block type tooth autogenous bone graft successfully stimulated mesenchymal cells leading to endochondral ossification and bone regeneration. We observed that the powered bone graft material which is acid insoluble especially is preferable as a carrier for BMP-2.

scholarly journals Effectiveness of Silicon Platelet-Rich Fibrin and Autologous Bone on Bone Regeneration in Rabbit Calvarian Defects: A Radiological and Histological Study

2021 ◽  
Vol 11 (9) ◽  
pp. 4074
Author(s):  
Argimiro Hernández-Suarez ◽  
María Rizo-Gorrita ◽  
Dubraska Suárez-Vega ◽  
Gladys Velazco ◽  
Ivan Rodriguez Gelfenstein ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Oral Surgery ◽  
Histological Study ◽  
Study Groups ◽  
Control Group ◽  
Graft Material ◽  
Platelet Rich Fibrin ◽  
Computed Microtomography ◽  
Autologous Bone ◽  
Group 2

Repairing bone defects in oral surgery often requires the use of bone regeneration techniques. Silicon is an element that has been employed as regeneration material in several studies. In our study, silicon was combined with autologous bone and platelet-rich fibrin (PRF) membranes to analyse the behaviour of this element in bone regeneration. Four circumferential defects were created in the cranial vault of five New Zealand rabbits. The following elements were applied to the regeneration of the defects: (P): PRF; (S): silicon and (B): autologous bone, with the following distribution of study groups: Group 1 (PSB); Group 2 (PS); Group 3 (SB) and Group 4 (CONTROL): unregenerate group. The animals were sacrificed after 3 weeks. Computed microtomography studies (μ-CT) were carried out, as well as histomorphometric ones. The ANOVA statistical test was used with a Bonferroni post-hoc test to compare the results (p ≤ 0.05). Radiologically, groups PSB and SB were better as far as quantity and percentage of healthy bone observed, but not significantly compared to the control group. The PS group was significantly worse. The histological test revealed that the PSB group was the one to present the largest area, percentage and perimeter of mineralised bone. On evaluating the forming bone (osteoid), no difference was observed across the groups with the exception of the bone perimeter, where the SB group was significantly better. The bone height variable showed no significant differences. In conclusion we can state that the combination of PRF, autologous bone and silicon provides good results at 3 weeks whilst the PS group shows the worst results. This highlights the importance of autologous bone forming part of the graft material in order for the bone to mineralise.

scholarly journals Morphology of autogenous bone graft and castor oil polyurethane in the infraorbital rim of rabbits: a comparative study

2006 ◽  
Vol 21 (5) ◽  
pp. 341-347 ◽  
Author(s):  
José Carlos Garcia de Mendonça ◽  
Rafael De Rossi ◽  
Celso Massaschi Inouye ◽  
Diego Rodrigo Paulillo Bazan ◽  
João Carlos Castro Monteiro ◽  
...  
Keyword(s):  
Bone Graft ◽  
Castor Oil ◽  
Bone Repair ◽  
Bone Defects ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Control Group ◽  
Cell Nuclei ◽  
Zygomatic Bone ◽  
Chi Squared

PURPOSE: Morphological study comparing castor oil polyurethane and autogenous bone graft to repair bone defect in zygomatic bone of rabbits. METHODS: Twenty-four adult, male New Zealand rabbits were randomly distributed between two groups of twelve. Bone defects of 5mm in diameter were cut through the zygomatic bone and filled with polyurethane discs in the experimental group or autogenous bone harvested from the tibia in the control group. Animals were sacrificed after 30, 60 or 90 days, and the zygomatic bones were macro- and microscopically analyzed. Student's, Fisher's, chi-squared and McNemar's tests were used for statistical analysis. RESULTS: Both the castor oil polyurethane and the autograft adapted well to the defect, with no need for fixation. Fibrous connective tissue encapsulated the polyurethane, but no inflammation or giant cell reaction was observed. Acidophilic and basophilic areas were observed inside the micropores of the polyurethane, suggesting cell nuclei. After 90 days, bone repair with a lamellar pattern of organization was observed in the control group. CONCLUSION: The castor oil polyurethane was biocompatible and did not cause inflammation. It may be considered an alternative to fill bone defects.

The Effect of Autogenous Bone Graft Mixed With Recombinant Human Vascular Endothelial Growth Factor on Bone Regeneration

2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Ufuk Tasdemir ◽  
Berrin İyilikçi ◽  
Melisa Cansu Aktürk ◽  
Özmen Ozmen ◽  
Alper Kizildağ ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

Utilization of Ethyl Cyanoacrylate and 2-Octyl Cyanoacrylate Adhesives for Autogenous Bone Graft Fixation: Histomorphometric Study in Rats

2014 ◽  
Vol 40 (4) ◽  
pp. 411-417 ◽  
Author(s):  
Jônatas Caldeira Esteves ◽  
Jônatas Mattos Monteiro ◽  
Alessandra Marcondes Aranega ◽  
Walter Betoni Junior ◽  
Celso Koogi Sonoda
Keyword(s):  
Bone Graft ◽  
Inflammatory Infiltrate ◽  
Parietal Bone ◽  
Graft Fixation ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Control Group ◽  
Bone Fragment ◽  
Recipient Site ◽  
Ethyl Cyanoacrylate

The present study analyzes the repair process of autogenous bone graft in a block fixed with ethyl cyanoacrylate and 2-octyl cyanoacrylate adhesives in rat calvaria. Forty-eight rats, divided into 3 groups, received round osteotomies at the right parietal bone for the attainment of autogenous bone graft fragment, which was fixed at the opposite side to the donor site with ethyl cyanoacrylate (ethyl group) and 2-octyl cyanoacrylate (octyl group) adhesives. In the control group, bone fragment was only juxtaposed at the parietal bone surface without any fixation material. The animals were euthanized after 10 and 60 postoperative days. The calvariae were processed in a laboratory for the attainment of slides stained through the hematoxylin and eosin technique for histological and histometric analysis. The qualitative analysis showed a discrete inflammatory infiltrate in the control group and moderate inflammatory infiltrate in the ethyl and octyl groups at the 10-day period, which remained at the 60-day period, mainly in the octyl group. The bone fragment remained bonded to the recipient site through the adhesive, but graft incorporation was not observed in any of the specimens. Resorption was higher in the octyl group followed by the ethyl and control groups, both at the 10- and 60-day periods, but with no statistical significance (P < .05). Although promoting graft fixation and its maintenance at the recipient site, both studied adhesives did not allow the graft incorporation, producing a localized and discrete inflammatory reaction, which persisted at 60 days, being more intense in the octyl cyanoacrylate group.

Effect of platelet-rich plasma on bone regeneration in autogenous bone graft

2004 ◽  
Vol 33 (1) ◽  
pp. 56-59 ◽  
Author(s):  
B.-H. Choi ◽  
C.-J. Im ◽  
J.-Y. Huh ◽  
J.-J. Suh ◽  
S.-H. Lee
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Platelet Rich Plasma ◽  
Autogenous Bone Graft ◽  
Autogenous Bone

scholarly journals The effect of autogenous tooth bone graft material without organic matter and type I collagen treatment on bone regeneration

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Min-Gu Kim ◽  
Jung-Han Lee ◽  
Gyoo-Cheon Kim ◽  
Dae-Seok Hwang ◽  
Chul-Hun Kim ◽  
...  
Keyword(s):  
Organic Matter ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Grafting ◽  
Type I Collagen ◽  
Treated Group ◽  
Control Group ◽  
Type I ◽  
Graft Material ◽  
Collagen Treatment

Abstract Objectives The aim of this study is to examine the effect of particulate autogenous tooth graft removed with organic matter and type I collagen addition on bone regeneration and to validate the possibility of useful allograft material for jaw defects. Material and methods Autogenous tooth bone maker (Korean Dental Solution® KOREA) made particulate autogenous tooth not including organic matter. We used to the developed tooth grafts for experiment. Cell adhesion test with hemacytometer and energy dispersive X-ray spectroscopy (Supra40 VP®, Carl Zeiss, Germany) analysis about the particulate autogenous tooth and type I collagen were performed. Rabbits were divided into three groups: bone graft with organic matter (OM) removing particulate autogenous tooth group, bone graft with OM removing particulate autogenous tooth and type I collagen group, and a control group. Bone grafting was performed in rabbit’s calvaria. The rabbits were sacrificed at different interval at 1, 2, 4, and 6 weeks after bone grafting for the histopathologic observation and observed the effect of bone regeneration by SEM, H-E & Masson stains, osteocalcin IHC staining. Result In vitro cytopathological study showed affinity for cells, cell attachment pattern, and cell proliferation in the order of control group, OM-removed and collagen-treated group, OM-removed particulate autogenous tooth group. The results of the degree of mineralization were opposite to those of the previous cell experimental results, and the OM-removed group, OM-removed group and collagen-treated group were relatively higher than the control group. Histopathologic analysis showed that vascularization and neonatal bone formation were higher in particulate autogenous tooth group with removing OM and with addition of collagen than control group and group of OM removed only. Immunohistochemical analysis showed that osteocalcin (OSC) expression was not observed in the control group, but at 4 weeks groups, OSC expression was observed the OM removed and OM-removed-collagen-treated particulate autogenous tooth, and the degree of expression was somewhat stronger in group of the OM removed and collagen additionally treated particulate autogenous tooth. Conclusion Particles that do not contain organic matter, the saint tooth, was responsible for sufficient bone graft material through the role of space maintenance and bone conduction, and further improved bone formation ability through additional collagen treatment. Therefore, research on various extracellular substrates and autologous bone grafting materials is necessary, and through this, it is possible to lay the foundation for a new type of autologous bone grafting material with excellent academic and technical utility.

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