IGF-1R Expression in Normal Breast Tissue, Benign, Proliferative, In-Situ Breast Lesions, and Invasive Breast Carcinoma.

2009 ◽  
Author(s):  
R. Bhargava ◽  
S. Beriwal ◽  
D. Dabbs
Keyword(s):  
Breast Carcinoma ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Invasive Breast Carcinoma ◽  
Breast Lesions

scholarly journals Novel Use for DOG1 in Discriminating Breast Invasive Carcinoma from Noninvasive Breast Lesions

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Henghui Cheng ◽  
Shouhua Yang ◽  
Zhiling Qu ◽  
Sheng Zhou ◽  
Qiurong Ruan
Keyword(s):  
Breast Carcinoma ◽  
Breast Tissue ◽  
Invasive Carcinoma ◽  
Malignant Tumors ◽  
Myoepithelial Cells ◽  
Invasive Breast Carcinoma ◽  
Breast Lesions ◽  
Human Breast ◽  
Stromal Tumors ◽  
Muscle Myosin

Aims. DOG1 has proven to be a useful marker of gastrointestinal stromal tumors (GISTs). Recently, DOG1 expression has also been reported in some non-GIST malignant tumors, but the details related to DOG1 expression in breast tissue remain unclear. The aim of this study was to detect the expression of DOG1 in the human breast and to evaluate the feasibility of using DOG1 to discriminate between invasive breast carcinoma and noninvasive breast lesions.Methods and Results. A total of 210 cases, including both invasive and noninvasive breast lesions, were collected to assess DOG1 expression immunohistochemically. DOG1 expression was consistently positive in breast myoepithelial cells (MECs), which was similar to the results obtained for three other MEC markers: calponin, smooth muscle myosin heavy chain (SMMHC), and P63 (P>0.05in all). Importantly, DOG1 was useful in discriminating invasive breast carcinoma from noninvasive breast lesions (P<0.05).Conclusions. DOG1 is a useful marker of breast MECs, and adding DOG1 to the MEC identification panel will provide more sophisticated information when diagnosing uncertain cases in the breast.

Localization of hyaluronan in normal breast tissue, radial scar, and tubular breast carcinoma

1993 ◽  
Vol 24 (12) ◽  
pp. 1294-1297 ◽  
Author(s):  
Manuel de la Torre ◽  
Alvin F. Wells ◽  
Jonas Bergh ◽  
Anders Lindgren
Keyword(s):  
Breast Carcinoma ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Radial Scar ◽  
Tubular Breast

scholarly journals Changes in immune Cell Types in Breast Suggest a Decline in Immune Surveillance and Increased Immunosuppression with Age

2021 ◽  
Author(s):  
Arrianna Zirbes ◽  
Jesuchristopher Joseph ◽  
Jennifer C Lopez ◽  
Rosalyn W Sayaman ◽  
Mudaser Basam ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Epithelial Cell ◽  
Peripheral Blood ◽  
Breast Tissue ◽  
Immune Cell ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Cell Populations

Abstract Background : A majority of breast cancers (BC) are age-related and we seek to determine what cellular and molecular changes occur in breast tissue with age that make women more susceptible to cancer initiation. Immune-epithelial cell interactions are important during mammary gland development and the immune system plays an important role in BC progression. The composition of human immune cell populations is known to change in peripheral blood with age and in breast tissue during BC progression. Less is known about changes in immune populations in normal breast tissue and how their interactions with mammary epithelia change with age. Methods : We quantified densities of T cells, B cells, and macrophage subsets in pathologically normal breast tissue from 122 different women who ranged in age from 24 to 74 years old. Donor-matched peripheral blood from a subset of 20 donors was analyzed by flow cytometry. Tissue immune cell densities and localizations relative to the epithelium were quantified in situ with machine learning-based analyses of multiplex immunohistochemistry-stained tissue sections. In situ results were corroborated with flow cytometry analyses of peri-epithelial immune cells from primary organoid preparations and transcriptome analyses of public data from bulk tissue reduction mammoplasties. Results : Proportions of immune cell subsets in breast tissue and donor-matched peripheral blood were not correlated. Density (cells/mm 2 ) of T and B lymphocytes in situ decreased with age. T cells and macrophages preferentially localized near or within epithelial bilayers, rather than the intralobular stroma. M2:M1 macrophage ratio increased with age and was accompanied by an increased density of M2 in the intralobular stroma. Transcriptional signature analyses suggested age-dependent decline in adaptive immune cell populations and functions and increased innate immune cell activity. Conclusions : T cells and macrophages are so intimately associated with the epithelia that they are embedded within the bilayer, suggesting an important role for immune-epithelial cell interactions. Age-associated decreased T cell density in peri-epithelial regions, and increased M2 macrophage density in intralobular stroma suggests the emergence of a tissue microenvironment that is simultaneously immune-senescent and immunosuppressive with age .

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