Urinary Metabolites of a Tobacco-Specific Lung Carcinogen in Nonsmoking Hospitality Workers

SummaryThe metabolism of vitamin K1- 14C and menadione-14C (vitamin K3-14C) was studied in normal and hepateetomized rats. After the administration of menadione, about 70% of the 14C was excreted in the urine in 24 hrs in both types of rats. Two urinary metabolites were identified by enzymatic hydrolysis: one a glucuronide and the other a sulfate of reduced menadione. Thus, the liver is not necessary for the metabolism of menadione. In the vitamin K1 studies, the intact rats excreted only 10% of the 14C and the hepatectomized rats excreted less than 0.5%. The retention of vitamin K1 may explain its superiority over menadione as an antidote for overdosages of oral anticoagulants.

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Kepuasan Masyarakat Terhadap Pelayanan Kelurahan Setelah Pemekaran Di Tangerang Selatan

Liquidity ◽

10.32546/lq.v2i2.117 ◽

2018 ◽

Vol 2 (2) ◽

pp. 151-159

Author(s):

Pitri Yandri

Keyword(s):

Public Perception ◽

Service Personnel ◽

Urban Services ◽

Hospitality Workers ◽

Care Workers ◽

Before And After ◽

Cartesian Diagram ◽

Better Than

The purpose of this study is (1) to analyze public perception on urban services before and after the expansion of the region, (2) analyze the level of people's satisfaction with urban services, and (3) analyze the determinants of the variables that determine what level of people's satisfaction urban services. This study concluded that first, after the expansion, the quality of urban services in South Tangerang City is better than before. Secondly, however, public satisfaction with the services only reached 48.53% (poor scale). Third, by using a Cartesian Diagram, the second priority that must be addressed are: (1) clarity of service personnel, (2) the discipline of service personnel, (3) responsibility for care workers; (4) the speed of service, (5) the ability of officers services, (6) obtain justice services, and (7) the courtesy and hospitality workers.

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PHENOLIC METABOLITES OF DL-NORGESTREL: A METHOD FOR THE REMOVAL OF 1-HYDROXYLATED METABOLITES, POTENTIAL SOURCES OF PHENOLIC ARTIFACTS

Acta Endocrinologica ◽

10.1530/acta.0.0760789 ◽

1974 ◽

Vol 76 (4) ◽

pp. 789-800 ◽

Cited By ~ 7

Author(s):

Samuel F. Sisenwine ◽

Ann L. Liu ◽

Hazel B. Kimmel ◽

Hans W. Ruelius

Keyword(s):

Urinary Metabolites ◽

Ion Exchange Resin ◽

Gas Chromatography Mass Spectrometry ◽

Phenolic Metabolites ◽

Hydroxylated Metabolites ◽

Analytical Work ◽

Potential Sources ◽

Alkaline Conditions ◽

Artifact Formation ◽

Work Up

ABSTRACT The identification of 1β-hydroxynorgestrel among the urinary metabolites of dl-norgestrel and the facile transformation of this compound under mild alkaline conditions to a potentially oestrogenic phenol provide an experimental basis for the conclusion advanced by others that the oestrogens present in the urine of subjects treated with synthetic progestens are artifacts formed during analytical work-up. A method has been devised which eliminates 1-hydroxylated metabolites as potential sources of phenolic artifacts. This method is based on the reduction by NaBH4 of the 1-hydroxy-4-en-3-one grouping in the A ring thereby excluding the possibility of aromatization during later fractionation on a basic ion exchange resin that separates neutral from phenolic metabolites. In the urines of women treated with 14C-dl-nogestrel, only 0.17–0.27% of the dose is found to have phenolic properties when this method is used. Two of the phenolic metabolites, 18-homoethynyloestradiol and 16β-hydroxy-18-homoethynyloestradiol, are present in amounts smaller than 0.01 % of the dose. Without the reduction steps the percentages are noticeably higher, indicating artifact formation under alkaline conditions. Similar results were obtained with urines from African Green Monkeys (Cercopithecus Aethiops) that had been dosed with 14C-dl-norgestrel. Radiolabelled 18-homoethynyloestradiol and 16β-hydroxy-18-homoethynyloestradiol were isolated from monkey urine and their identity confirmed by gas chromatography-mass spectrometry.

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Urinary Metabolites of Hydrazine in Male Fischer 344 Rats Following Inhalation or Intravenous Exposure

10.21236/ada170743 ◽

1986 ◽

Cited By ~ 1

Author(s):

Beth M. Llewellyn ◽

William C. Keller ◽

Carl T. Olson

Keyword(s):

Urinary Metabolites ◽

Fischer 344 Rats ◽

Fischer 344

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Urinary metabolites of thromboxane and prostacyclin in diabetes mellitus

Acta Endocrinologica ◽

10.1530/acta.0.1180301 ◽

1988 ◽

Vol 118 (2) ◽

pp. 301-305 ◽

Cited By ~ 6

Author(s):

K. Gréen ◽

O. Vesterqvist ◽

V. Grill

Keyword(s):

Diabetes Mellitus ◽

Mass Spectrometry ◽

Gas Chromatography ◽

Insulin Treatment ◽

Artificial Pancreas ◽

Urinary Metabolites ◽

Gas Chromatography Mass Spectrometry ◽

Diabetic State ◽

In Vivo Synthesis

Abstract. The in vivo synthesis of thromboxane A2 and prostacyclin was estimated in 23 diabetics through measurements of the major urinary metabolites 2,3-dinor-thromboxane B2 and 2,3-dinor-6-keto-PGF1α utilizing gas chromatography-mass spectrometry. Mean excretion was similar to that in non-diabetic subjects. The possible influence of hyperglycemia on the excretion of 2,3-dinor-thromboxane B2 and 2,3-dinor-6-keto-PGF1α was evaluated in three ways: by measuring excretion before and during an acute 9-h normalization of hyperglycemia through an artificial pancreas (Biostator) as well as by comparing excretion before and 7–12 days or 40–180 days after the initiation of insulin treatment. Despite significant reducing effects on hyperglycemia or on levels of hemoglobin A1c, no effects on the excretion of the thromboxane and prostacyclin metabolites could be found. Abnormal formation of thromboxane or prostacyclin is not a generalized feature of the diabetic state.

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Elevated levels of urine isocitrate, hydroxymethylglutarate, and formiminoglutamate are associated with arterial stiffness in Korean adults

Scientific Reports ◽

10.1038/s41598-021-89639-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ji-Hee Haam ◽

Young-Sang Kim ◽

Doo-Yeoun Cho ◽

Hyejin Chun ◽

Sang-Woon Choi ◽

...

Keyword(s):

Risk Factors ◽

Cardiovascular Risk ◽

Arterial Stiffness ◽

Pulse Wave Velocity ◽

Cardiovascular Risk Factors ◽

Wave Velocity ◽

Pulse Wave ◽

Urinary Metabolites ◽

Metabolic Disturbances ◽

Liquid Chromatograph

AbstractRecent evidence suggests that cellular perturbations play an important role in the pathogenesis of cardiovascular diseases. Therefore, we analyzed the association between the levels of urinary metabolites and arterial stiffness. Our cross-sectional study included 330 Korean men and women. The brachial-ankle pulse wave velocity was measured as a marker of arterial stiffness. Urinary metabolites were evaluated using a high-performance liquid chromatograph-mass spectrometer. The brachial-ankle pulse wave velocity was found to be positively correlated with l-lactate, citrate, isocitrate, succinate, malate, hydroxymethylglutarate, α-ketoisovalerate, α-keto-β-methylvalerate, methylmalonate, and formiminoglutamate among men. Whereas, among women, the brachial-ankle pulse wave velocity was positively correlated with cis-aconitate, isocitrate, hydroxymethylglutarate, and formiminoglutamate. In the multivariable regression models adjusted for conventional cardiovascular risk factors, three metabolite concentrations (urine isocitrate, hydroxymethylglutarate, and formiminoglutamate) were independently and positively associated with brachial-ankle pulse wave velocity. Increased urine isocitrate, hydroxymethylglutarate, and formiminoglutamate concentrations were associated with brachial-ankle pulse wave velocity and independent of conventional cardiovascular risk factors. Our findings suggest that metabolic disturbances in cells may be related to arterial stiffness.

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