Abstract 1722: c-Met overexpression contributes to the acquired apoptotic resistance of non-adherent ovarian cancer cells through a cross-talk mediated by phosphatidylinositol 3-kinase and extracellular signal-regulated kinase 1/2

2010 ◽  
Author(s):  
Maggie K. S. Tang ◽  
Hong Y. Zhou ◽  
Judy W. P. Yam ◽  
Alice S. T. Wong
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Cross Talk ◽  
Ovarian Cancer Cells ◽  
Phosphatidylinositol 3 Kinase ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal ◽  
Phosphatidylinositol 3

Baicalein Induces Beclin 1- and Extracellular Signal-Regulated Kinase-Dependent Autophagy in Ovarian Cancer Cells

2017 ◽  
Vol 45 (01) ◽  
pp. 123-136 ◽  
Author(s):  
Ya-Fang Wang ◽  
Yu-Lian Xu ◽  
Zheng-Hai Tang ◽  
Ting Li ◽  
Le-Le Zhang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Viability ◽  
Cancer Cells ◽  
Combined Treatment ◽  
Beclin 1 ◽  
Ovarian Cancer Cells ◽  
Extracellular Signal Regulated Kinase ◽  
Ovarian Cancer Cell Lines ◽  
Extracellular Signal ◽  
Anti Oxidant

Baicalein (BA), one of the major compounds isolated from the root of Scutellaria baicalensis Gerogi, exhibits various pharmacological effects, such as anti-oxidant, anti-inflammatory, and anticancer effects. In this study, we found that BA reduced cell viability and increased apoptosis in ovarian cancer cells. Treatment of cells with BA enhanced microtubule-associated protein light chain 3-II (LC3-II) expression, acidic vesicular organelle and GFP-LC3 fluorescence dot accumulation. Combined treatment with chloroquine and BA apparently reduced cell viability and increased the cleavage of poly (ADPribose) polymerase (PARP) in both HEY and A2780 ovarian cancer cell lines, indicating that BA induces a protective autophagy in these cells. Knockdown of Beclin 1 by siRNA remarkably decreased BA-induced LC3-II lipidation. In addition, we found an increase in the phosphorylation of extracellular signal-regulated kinase (ERK, Thr202/Thr204) and AKT (Ser473) after BA treatment, and inhibition of ERK activation by the pharmacological inhibitor U0126 or ERK siRNA blocked BA-induced autophagy. Taken together, these results suggest that BA induces Beclin 1- and ERK-dependent autophagy in ovarian cancer cells.

Sign in / Sign up

Export Citation Format

Share Document