Abstract 3024: FGFR1 amplification is linked to the squamous cell carcinoma subtype in esophageal carcinoma.

The high incidence of esophageal squamous cell carcinoma has been reported in selected ethnic populations including North of Iran. Low survival rate of esophageal carcinoma is partially due to the presence of stem-like cancer cells with chemotherapy resistance. In the current study, we aimed to determine the effects of auraptene, an interesting dietary coumarin with various biological activities, on malignant properties of stem-like esophageal squamous cell carcinoma, in terms of sensitivity to anticancer drugs and expression of specific markers. To do so, the half maximal inhibitory concentration values of auraptene, cisplatin, paclitaxel, and 5-fluorouracil were determined on esophageal carcinoma cells (KYSE30 cell line). After administrating combinatorial treatments, including nontoxic concentrations of auraptene + cisplatin, paclitaxel, or 5-fluorouracil, sensitivity of cells to chemical drugs and also induced apoptosis were assessed. In addition, quantitative real-time polymerase chain reaction was used to study changes in the expression of tumor suppressor proteins 53 and 21 ( P53 and P21), cluster of differentiation 44 ( CD44), and B cell-specific Moloney murine leukemia virus integration site 1 ( BMI-1) upon treatments. Results of thiazolyl blue assay revealed that auraptene significantly ( P < .05) increased toxicity of cisplatin, paclitaxel, and 5-fluorouracil in KYSE30 cells, specifically 72 hours after treatment. Conducting an apoptosis assay using flow cytometry also confirmed the synergic effects of auraptene. Results of quantitative real-time polymerase chain reaction revealed significant ( P < .05) upregulation of P53 and P21 upon combinatorial treatments and also downregulation of CD44 and BMI-1 after auraptene administration. Current study provided evidence, for the first time, that auraptene attenuates the properties of esophageal stem-like cancer cells through enhancing sensitivity to chemical agents and reducing the expression of CD44 and BMI-1 markers.

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Expressions of HPV 16-E6 in esophageal carcinoma and it's clinical significance

Asian Journal of Medical Sciences ◽

10.3126/ajms.v6i6.12537 ◽

2015 ◽

Vol 6 (6) ◽

pp. 39-42

Author(s):

Xing Zhao ◽

Rajina Sahi ◽

Yu-Yang Zhao ◽

Jun Wang ◽

Chun- Hui Li

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Immunohistochemical Method ◽

Esophageal Mucosa ◽

Medical Sciences ◽

Hpv 16 ◽

Hpv16 E6

Aim: To explore the association between HPV16-E6 protein and esophageal squamous cell carcinoma.Materials and Methods: SP immunohistochemical method was used to examine the expression of HPV 16-E6 in 50 cases of esophageal squamous cell carcinoma, 10 cases of normal esophageal squamous cell and 10 cases of adjacent tissue.Results: The expressions of HPV 16-E6 was significantly higher in esophageal carcinoma than in normal esophageal mucosa and in adjacent tissue. The expressions of HPV 16-E6 had correlation with invasive depth (P<0.05), but not with patient age, lymph node metastasis, tumor size (P>0.05).Conclusion: HPV 16-E6 can promote the growth and metastasis of esophageal squamous cell carcinoma and can be a prognostic factor of esophageal squamous cell carcinoma. DOI: http://dx.doi.org/10.3126/ajms.v6i6.12537 Asian Journal of Medical Sciences Vol.6(6) 2015 39-42

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Clinical, epidemiological, and diagnostic characteristics of esophageal carcinoma in a Pakistani population

Annals of Saudi Medicine ◽

10.5144/0256-4947.2021.91 ◽

2021 ◽

Vol 41 (2) ◽

pp. 91-100

Author(s):

Muhammad Sohaib Asghar ◽

Noman Ahmed Khan ◽

Syed Jawad Haider Kazmi ◽

Maira Hassan ◽

Uzma Rasheed ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Cancer ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Squamous Cell Carcinomas ◽

Care Hospital ◽

Female Ratio ◽

Luminal Narrowing ◽

Male To Female

BACKGROUND: Esophageal cancer ranks eighth among the most prevalent cancers globally and is the sixth leading cause of mortality from malignancy worldwide; it is the 7th most prevalent malignancy in males and the 6th most prevalent malignancy in females. In Pakistan, the incidence is 4.1 per 100 000 with the province of Baluchistan having the greatest incidence. OBJECTIVE: Report trends and characteristics of esophageal cancer in Pakistan over the past 10 years. DESIGN: Cross-sectional, retrospective review of medical records. SETTING: Tertiary care hospital. PATIENTS AND METHODS: The study included all patients admitted with a diagnosis of esophageal carcinoma with a mass lesion or luminal narrowing. The records were for the period from January 2011 to September 2020. MAIN OUTCOME MEASURES: Gender, histopathological types/differentiation along with clinical/laboratory findings. SAMPLE SIZE: 1009 with a mean (standard deviation) age of 49.3 (14.2) and a median (interquartile range of 50 (22) years (443 males and 566 females with age of 51.0 [20] years and 47.9 [23.8] years, respectively). The male-to-female ratio was 1:1.2. RESULTS: Most patients (82.7%) had squamous cell carcinomas with a male-to-female ratio of 1:2; the remainder had adenocarcinomas with a male-to-female ratio of 4:1 ( P <.001). Dysphagia, weight loss, and vomiting were the most prevalent symptoms. More adenocarcinoma masses were located distally compared with squamous cell carcinomas ( P =.030), lesions were most likely to be ulcerated ( P =.910). Luminal narrowing was slightly more frequent in squamous cell carcinoma ( P =.215), thickening was more prominently circumferential in the adenocarcinomas. In squamous cell carcinoma, the most common variant was moderately differentiated while moderate to poorly differentiated variants were more common in adenocarcinoma. In the survival analysis, squamous cell carcinoma ( P =.014 vs adenocarcinoma), particularly the well-differentiated type ( P =.018 vs other variants), projected a better prognosis. CONCLUSION: Our study reports the most recent trends of esophageal carcinoma in this region. LIMITATIONS: Lack of metastatic workup, TNM staging, and mode of treatment, along with the overlapping pattern of histological variants. CONFLICT OF INTEREST: None.

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A Case of Double Esophageal Carcinoma Along with So-called Carcinosarcoma and Squamous Cell Carcinoma

The Japanese Journal of Gastroenterological Surgery ◽

10.5833/jjgs.38.1296 ◽

2005 ◽

Vol 38 (8) ◽

pp. 1296-1300 ◽

Cited By ~ 2

Author(s):

Katsuhiro Okuda ◽

Masaaki Sano ◽

Hiroshi Narita ◽

Tadashi Shibata ◽

Katsumi Kato ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell

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Histological pattern of esophageal cancer at BP Koirala memorial cancer hospital in Nepal: a three year retrospective study

Journal of Pathology of Nepal ◽

10.3126/jpn.v2i4.6877 ◽

2012 ◽

Vol 2 (4) ◽

pp. 277-281

Author(s):

CB Pun ◽

G Aryal ◽

R Basyal ◽

S Shrestha ◽

T Pathak ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Cancer ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Signet Ring Cell ◽

Cancer Center ◽

Common Site ◽

Histological Pattern ◽

Cancer Hospital

Background: The incidence of esophageal adenocarcinoma is increasing in trends. Squamous cell carcinoma is associated with tobacco and alcohol consumption. Adenocarcinoma is often associated with a history of gastroesophageal reflux disease and Barrett's esophagus. The aim of this study was to find out the histological pattern of esophageal cancer in one of the largest Cancer center in Nepal.Materials and Methods: Between January 2008 and December 2011, a total of 106 cases of esophageal cancer were received in the department of pathology, BP Koirala Memorial Cancer Hospital. Relevant clinical data were retrieved from computer database of the hospital.Results: A total of 106 cases of esophageal carcinomas were diagnosed during a three years period. There were 68 (64.15%) cases of squamous cell carcinoma, 33 (31.13%) cases of adenocarcinoma including signet ring cell carcinoma, 4 (3.76%) cases of undifferentiated carcinoma and 1 (0.94%) case of small cell carcinoma. The esophageal cancer was most common in the age group of 61-70 years of age. Distal third of esophagus was the most common site for esophageal carcinoma, followed by middle esophagus and proximal esophagus.Conclusion: The most frequent type of esophageal carcinoma is squamous cell carcinoma followed by adenocarcinoma. Distal esophagus is the most common site with male preponderance.Journal of Pathology of Nepal (2012) Vol. 2, 277-281DOI: http://dx.doi.org/10.3126/jpn.v2i4.6877

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Protein-coding genes combined with long non-coding RNAs predict prognosis in esophageal squamous cell carcinoma patients as a novel clinical multi-dimensional signature

Molecular BioSystems ◽

10.1039/c6mb00585c ◽

2016 ◽

Vol 12 (11) ◽

pp. 3467-3477 ◽

Cited By ~ 15

Author(s):

Jin-Cheng Guo ◽

Chun-Quan Li ◽

Qiu-Yu Wang ◽

Jian-Mei Zhao ◽

Ji-Yu Ding ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Mortality Rate ◽

Esophageal Squamous Cell Carcinoma ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Gastrointestinal Cancers ◽

Protein Coding ◽

Protein Coding Genes ◽

Non Coding Rnas

Esophageal carcinoma is one of the most malignant gastrointestinal cancers worldwide, and has a high mortality rate.

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FGFR1 Amplification Is Often Homogeneous and Strongly Linked to the Squamous Cell Carcinoma Subtype in Esophageal Carcinoma

PLoS ONE ◽

10.1371/journal.pone.0141867 ◽

2015 ◽

Vol 10 (11) ◽

pp. e0141867 ◽

Cited By ~ 10

Author(s):

Katharina von Loga ◽

Jule Kohlhaussen ◽

Lia Burkhardt ◽

Ronald Simon ◽

Stefan Steurer ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell

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Frequency of Esophageal Carcinoma and Delay in Diagnostic Workup; A Multi-centre Experience from Southern Pakistan

10.5742/mewfm.2021.93986 ◽

2021 ◽

Vol 19 (2) ◽

Author(s):

Mohsin Javed ◽

Irtiza Ahmed Bhatti ◽

Nazish Jaffar ◽

Ghulam Haider ◽

Zareen Irshad ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Late Stage ◽

Delayed Diagnosis ◽

Diagnostic Workup ◽

Contributing Factors ◽

General Physician ◽

Cross Sectional

Objective: Esophageal carcinoma is one of the most lethal and yet least studied malignancies in South Asian region. The aim of our study was to determine the frequency of Esophageal Carcinoma and to identify factors responsible for late stage diagnosis of this malignancy. Methods: A multi-institutional prospective cross-sectional study was conducted at Oncology department, JPMC and Atomic Energy Medical Centre, Karachi. Association between risk factors of delayed diagnosis with ethnic groups, grade and stage of tumour was statistically assessed. Results: A frequency of 3.83% of esophageal cancer was recorded. Squamous cell carcinoma as the most common (80.6%) subtype, moderately differentiated grade in 67.7% and lower 1/3rd involvement was noted in 53.8% cases. Representation of Sindhi speaking ethnicity was 33.3%. First visit to a general physician was within a month of onset of symptoms in 54.8% patients. In 21.5%, first visit was delayed by more than 2 months. Biopsy was delayed in 19.4% cases. Significant association was found between delayed diagnosis and late referral to oncologist (P 0.003), non-availability of laboratory (P 0.018), location of tumour (P 0.000), age and size of tumour (P 0.001). Conclusion: Esophageal carcinoma is a common malignancy with squamous cell carcinoma as the most common subtype. Initial diagnosis of most of the patients was made in the late stage. Important contributing factors of late diagnosis were unawareness of patients, delayed initial diagnostic workup, poor socioeconomic status and late referrals to oncologists by General Physicians. No significant association was observed between ethnicity and grading or staging of tumour. Key words: Esophageal carcinoma, Esophageal Squamous Cell Carcinoma (SCC), Adenocarcinoma of Esophagus (AC), Southern Pakistan

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